High-fat-content insect DNA living body extraction method

By injecting normal saline into the back of insects with high fat content and heat treatment, the problems of low success rate and high lethality rate of live DNA extraction in insects are solved, and the DNA extraction effect with high success rate and low lethality rate is achieved.

CN119752881APending Publication Date: 2025-04-04QINGDAO AGRI UNIV
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Patent Information

Application Number
CN202510033209.6
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-01-09
Publication Date
2025-04-04

AI Technical Summary

Technical Problem

The existing methods for live insect DNA extraction are low in success and mortality among insects with high fat content, making it difficult to ensure the success of DNA extraction and the survival of insects.

Method used

DNA was extracted by injecting normal saline into the insect's back, body fluids were collected and heat treated with a mixed solution of STE/TNE buffer and protease K. The method includes specific steps of microscope operation, disinfection of injection needles, accurate injection, and heat treatment.

Benefits of technology

The success rate of live DNA extraction in insects with high fat content and the survival rate of insects is improved, and the problems of low success rate and high lethality rate in the prior art are solved, and a DNA extraction method with good repeatability and simple operation is provided.

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Abstract

The invention discloses a high-fat-content insect DNA living body extraction method which comprises the following steps: S1, taking an insect, and injecting normal saline into the back of the insect by using a disinfected injection needle under a microscope; s2, collecting body fluid after the body fluid of the insects seeps out; and S3, adding the collected body fluid into the DNA extracting solution, uniformly mixing, and then carrying out heat treatment to extract DNA. Aiming at the problem that it is difficult to successfully extract DNA for molecular analysis from living insects with high fat content, the invention provides a DNA extraction method which is high in success rate and simple to operate, the method is good in repeatability and high in success rate, the living insects still can normally survive after DNA is extracted, and the method can be used for subsequent corresponding in-depth research and has a wide application prospect. The problems of low success rate and high insect fatality rate of an insect living DNA extraction method in the prior art are solved.
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