A promoter for regulating oleic acid-specific expression in millet and its application

By screening resources of high oleic acid and low linoleic acid in millet, it was found that insertion fragments in the promoter region of the FAD2 gene resulted in a decrease in FAD2 gene expression, which solved the problem of low oleic acid content in millet, improved the antioxidant ability and thermal stability of processed products, and extended the shelf life.

CN119752911APending Publication Date: 2025-04-04GRAIN RES INST HEBEI ACAD OF AGRI & FORESTRY SCI +1
0 Cites 0 Cited by

Patent Information

Application Number
CN202510036332.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-01-09
Publication Date
2025-04-04

AI Technical Summary

Technical Problem

The existing millet varieties have low oleic acid content and high linoleic acid content, resulting in poor antioxidant and thermal stability of processed products and short shelf life.

Method used

By screening the southern millet resources with high oleic acid and low linoleic acid, it was found that there was a 352bp insertion fragment in the promoter region of the FAD2 gene in one of the resources, resulting in a decrease in FAD2 gene expression, an increase in oleic acid content, and a decrease in linoleic acid content.

Benefits of technology

The oleic acid content in millet is significantly improved and the linoleic acid content is reduced, the antioxidant ability and thermal stability of processed products are improved, and the shelf life is extended.

✦ Generated by Eureka AI based on patent content.
Patent Text Reader

Abstract

The invention discloses a promoter for regulating oleic acid-specific expression in millet and application thereof. The nucleotide sequence of the promoter is shown in SEQ NO.1. The molecular marker can identify the promoter type of millet FAD2 gene. The promoter type of the FAD2 gene is a promoter FAD2-(HapI) containing an insertion sequence or a promoter FAD2-(HapII) not containing an insertion sequence. Compared with millet with a promoter type of FAD2-(HapII), millet with a promoter type of FAD2-(HapI) has a lower expression amount of FAD2, an increased oleic acid content in grains, and a decreased linoleic acid content, and the result is a decreased linoleic acid ratio. The shelf life of millet processed products can be effectively increased, and millet quality can be improved. The molecular marker assists millet breeding and promotes the breeding of high-oleic acid and low-linoleic acid millet varieties.
Need to check novelty before this filing date? Find Prior Art