Application of mitochondrial GPAT1 gene and mitochondrial GPAT2 gene in regulation and control of plant cuticle thickness

By inhibiting the expression of mitochondrial GPAT1 and GPAT2 genes in Arabidopsis, the technical difficulties in regulating the thickness of the plant stratum corneum and the number of trichomes were solved, and the effective regulation of the stratum corneum and trichomes was achieved, and the drought tolerance and water metabolism ability of the plant were improved.

CN119753004AActive Publication Date: 2025-04-04JIANGSU ACAD OF AGRI SCI
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Patent Information

Application Number
CN202510128805.2
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-02-05
Publication Date
2025-04-04
Estimated Expiration
2045-02-05

AI Technical Summary

Technical Problem

The prior art has not yet disclosed whether mitochondrial GPAT is involved in plant keratin synthesis and regulating the development of trichomes, and there is a lack of effective methods to regulate plant keratin thickness and trichome numbers.

Method used

By inhibiting the expression of mitochondrial GPAT1 gene and mitochondrial GPAT2 gene in Arabidopsis, the thickness and number of stratum corneum of plant are reduced, thereby cultivating transgenic plants with thin stratum corneum and low-density stratum corneum.

Benefits of technology

The regulation of plant stratum corneum thickness, trichome number, water metabolism and drought tolerance is achieved, and new methods are provided for the cultivation of new plant varieties.

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Abstract

The invention relates to the technical field of gene engineering, in particular to application of a mitochondrial GPAT1 gene and a mitochondrial GPAT2 gene in regulating and controlling the thickness of a plant cuticle. The invention discovers that the mitochondrial GPAT1 gene and the mitochondrial GPAT2 gene have the effects of regulating and controlling the thickness of the cuticle, the number of hairy bodies, water metabolism and drought tolerance of the plant for the first time. When the mitochondrial GPAT1 gene and the mitochondrial GPAT2 gene are deleted, the synthesis of a cutin monomer is blocked, so that the cuticle of the double-mutant gpat1gpat2 of the arabidopsis thaliana is thinner than that of wild arabidopsis thaliana, the structure of the cuticle is damaged, the development of hairy bodies is blocked, and meanwhile, the water loss rate of seedlings of the double-mutant gpat1gpat2 of the arabidopsis thaliana is lower than that of the wild arabidopsis thaliana. Therefore, the mitochondrial GPAT1 gene and the mitochondrial GPAT2 gene can be used for regulating and controlling the thickness of the cuticle, the number of hairy bodies, water metabolism and drought tolerance of the plant.
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