A recombinant microorganism and its application in the fermentative production of ectoin

By optimizing the gene expression and metabolic path of E. coli, the problem of low production efficiency in microbial fermentation is solved, and the improvement of production and production efficiency is achieved, reducing costs.

CN120098885BActive Publication Date: 2025-08-01常州凯幸生物技术有限公司
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Patent Information

Application Number
CN202510602644.6
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-05-12
Publication Date
2025-08-01
Estimated Expiration
2045-05-12

AI Technical Summary

Technical Problem

During the existing microbial fermentation process, the rapid transfer of glucose leads to PEP consumption, production of by-product acetic acid and insufficient flux of aspartic acid pathway, which affects the yield and production efficiency of the acetic acid.

Method used

By expressing glucose from Zycomonas motility in E. coli, the gene glf promotes gloss Glf, reduces ptsG gene expression, overexpresses glucose kinase glk, and enhances the expression of phosphoenol pyruvate carboxylase PPC, while replacing the promoter of isocitrate dehydrogenase icd with a growth-dependent promoter, the production path of ergodogenics is optimized.

Benefits of technology

Without affecting the growth performance of bacteria, the yield and production efficiency of the dependents are significantly improved, and the cost of industrial production is reduced.

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Abstract

The present invention relates to the field of microbial technology, and specifically discloses a recombinant microorganism and its application in the fermentative production of ectoine. Compared with the starting strain, the recombinant microorganism of the present invention expresses the ectABC gene of Chromohalobacter salexigens, expresses the glucose facilitator gene of Zymomonas mobilis glf while reducing the ptsG gene expression, and also overexpresses glucokinase glk, preferably also overexpresses phosphoenolpyruvate carboxylase ppc, and more preferably replaces the original promoter of the isocitrate dehydrogenase gene icd with a growth-phase-dependent promoter. Without significantly affecting the growth performance of the bacterial cells, the recombinant microorganism of the present invention can significantly improve the yield and productivity of ectoine in fermentative production, thereby enhancing the efficiency of industrial production of ectoine and reducing the production cost.
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