Novel Cas12a nuclease BEST3 and application thereof

By obtaining and identifying the novel Cas12a protein BEST3, the problems of restricted PAM and low editing activity of the existing CRISPR-Cas12a system in gene editing and nucleic acid detection are solved, achieving wider target site selection and more efficient editing efficiency.

CN120173913APending Publication Date: 2025-06-20SHENZHEN HUADA GENE INST
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Patent Information

Application Number
CN202311773305.1
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2023-12-20
Publication Date
2025-06-20

AI Technical Summary

Technical Problem

The existing CRISPR-Cas12a system has problems such as restricted PAM, low editing activity and off-target effects in gene editing and nucleic acid detection, which limits its application scope and efficiency.

Method used

Through systematic mining and identification, a novel Cas12a protein is obtained, called BEST3, which has broader PAM recognition characteristics and improved nucleic acid cleavage activity, and is evaluated in combination with bioinformatics, biochemical and cytologic experiments.

Benefits of technology

BEST3 proteins show significant gene editing activity and nucleic acid detection capabilities in vivo and in vitro, providing a wider selection of target sites and more efficient editing efficiency, solving the problems of PAM restriction and low editing activity in existing systems.

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Abstract

The invention relates to the technical field of gene engineering, in particular to a novel Cas12a nuclease-BEST3 and application of the novel Cas12a nuclease-BEST3 in gene editing and nucleic acid detection. The invention provides a novel Cas12a protein, namely BEST3, which can be used as a gene editing tool enzyme and can be used for nucleic acid detection, more applicable tool enzymes are provided for in-vivo targeted gene editing and nucleic acid detection application of organisms, and a demonstrative experimental scheme or related biochemical characteristic analysis is provided for specific application.
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