O-type foot-and-mouth disease virus polyepitope virus-like particle nanogen and preparation method and application thereof

By expressing type O foot-and-mouth disease virus multi-epitope virus-like particle nanoantigens through CHO cell secretion, using HBc protein as a backbone, tandemly connecting antigenic epitopes, and simplifying the production process, the problems of uncertain immunization effects and high production costs in large animals in existing technologies have been solved, achieving efficient and low-cost industrial production and good immune protection effects.

CN120209160BActive Publication Date: 2026-07-24LANZHOU VETERINARY RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES(LANZHOU BRANCH CENTER OF CHINA ANIMAL HEALTH & EPIDEMIOLOGY CENTER)
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Patent Information

Application Number
CN202510274872.5
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-03-10
Publication Date
2026-07-24
Estimated Expiration
2045-03-10

AI Technical Summary

Technical Problem

Existing foot-and-mouth disease virus multi-epitope virus-like particle vaccines have uncertain efficacy in large animal immunization, high production costs, and complex production processes, making industrialization difficult.

Method used

A multi-epitope virus-like particle nanoantigen of type O foot-and-mouth disease virus was designed and expressed through secretion in CHO cells. Using HBc protein as a backbone, multiple antigenic epitopes were tandemly linked, and purification was achieved by sucrose gradient centrifugation, simplifying the production process.

Benefits of technology

The industrial production of O-type foot-and-mouth disease virus multi-epitope virus-like particle nanoantigens has been achieved with high efficiency and low cost. It has good immunization effect, protects immune animals such as pigs, cattle and sheep against viral attacks, and simplifies the production process.

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Abstract

The application discloses an O-type foot-and-mouth disease virus multi-epitope virus-like particle nano-antigen and a preparation method and application thereof. The nano-antigen is obtained by sequentially connecting antigen epitopes of three topological representative strains O / Tibet / CHA / 99, O / Mya98 / BY / 2010, OZK / 93, O / XJPS / CHA / 2017 and O / HKN / 2007 of the O-type foot-and-mouth disease virus in sequence, introducing a T cell epitope of a 3A gene of the foot-and-mouth disease virus at a C terminal, and finally inserting a woodchuck hepatitis B virus core antigen, and an amino acid sequence of the nano-antigen is shown as SEQ ID NO. 6. Immunological test results show that the nano-antigen has good antigenicity, a vaccine prepared from the nano-antigen can not only induce high-level protective antibodies, but also protect immunized animals against virus attack. In addition, the nano-antigen has immunoprotective effects on the O-type foot-and-mouth diseases of pigs, cattle and sheep. The application provides an effective technical means for preventing and controlling and purifying the foot-and-mouth disease.
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