Adaptive release exosome hydrogel, preparation method and application thereof

Abstract

This invention relates to the field of medical biomaterials technology, and particularly to an adaptive exosome-releasing hydrogel, its preparation method, and its applications. The hydrogel includes a precursor, a buffer solution, and exosomes. The precursor is dissolved in the buffer solution to form a matrix with a three-dimensional network structure, and the exosomes are loaded within this three-dimensional network structure. The precursor includes HA-ADH and OHA-TK-CP, with a mass ratio of HA-ADH to OHA-TK-CP of 1:5 to 1:1. In vitro and in vivo studies show that this hydrogel possesses excellent self-healing ability, significant antibacterial activity, efficient exosome release, and effective ROS reduction. Its synergistic anti-inflammatory and tissue regeneration-promoting effects accelerate wound healing in MRSA-infected diabetic rats, resulting in smaller scars after wound healing. The preparation method is simple to operate and uses mild reaction conditions, which is conducive to large-scale production.

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