Construction method of osteosarcoma-like organ model derived from patient

The osteosarcoma organoid model was constructed by combining scaffolding and matrix gel-free method with specific culture medium, which solved the problems of high cost and insufficient growth environment simulation in the existing technology, and achieved a high consistency and rapid growth osteosarcoma organoid model.

CN120442549APending Publication Date: 2025-08-08GUANGDONG HONG KONG MACAO GREATER BAY AREA PRECISION MEDICINE RESEARCH INSTITUTE (GUANGZHOU)
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Patent Information

Application Number
CN202510598304.0
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-05-09
Publication Date
2025-08-08

AI Technical Summary

Technical Problem

The existing osteosarcoma organoid model construction methods are costly and cannot effectively simulate the in vivo growth environment. In the prior art, the use of scaffolds or matrix gels is required for 3D culture, which increases the cost and lacks the mechanical stimulation environment for bone tumor growth.

Method used

The osteosarcoma organoid model was constructed by washing, digestion, filtration, centrifugation, resuspension and seed plate culture using the method of scaffolding without matrix gel, combined with shaker culture and specific complete medium, and cultured by ADMEM medium, fetal bovine serum FBS, double antibody, Glutmax, N-Ace, Nico, anti-apoptotic compounds Y27632, EGF, FGF2, FGF7, FGF10, RS-1 and Wnt.

Benefits of technology

In the absence of scaffolding and matrix gel, the cultured osteosarcoma organoid model has higher consistency with the microenvironment of bone-derived tumors, faster growth, and lower cost.

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Abstract

The invention belongs to the field of biological medicine, and particularly provides a construction method of a patient-derived osteosarcoma organ model, which comprises the steps of cleaning, digestion, filtration, centrifugation, resuspension and seed plate culture. The last step comprises the steps that a cell suspension starting plate is placed on a low-adhesion plate to be cultured, constant-temperature culture is kept in a shaking table for 5 days or above, a new complete culture medium is replaced every 1-3 days during the period, and the complete culture medium comprises an ADMEM culture medium, fetal calf serum FBS, double antibodies, Glutmax, N-Ac, Nico, anti-apoptosis compounds Y27632, EGF, FGF2, FGF7, FGF10, RS-1 and Wnt. According to the method, the osteosarcoma organoid model is cultured in a 3D manner in combination with the shaking table and the complete culture medium in a non-stent and non-matrigel state, and mechanical stimulation is generated during culture, so that the consistency of the obtained organoid and a bone-derived tumor microenvironment is higher, the quality of the organoid is higher, and the growth speed of the organoid is higher.
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