Propionibacterium metabolite containing gamma-Glu-ASP and preparation method of propionibacterium metabolite

By adding Asp and Glu to the propionibacterium culture medium to prepare γ-Glu-ASP propionibacterium metabolite, the problem in the existing technology that propionibacterium metabolites cannot impart excellent front and middle taste to food is solved, and the antibacterial activity is enhanced and the taste is rich, which is suitable for condiments and food additives.

CN120678200APending Publication Date: 2025-09-23EAST CHINA NORMAL UNIV +1
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Patent Information

Application Number
CN202511105296.8
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-08-07
Publication Date
2025-09-23

AI Technical Summary

Technical Problem

Existing technologies are unable to prepare Propionibacterium metabolites that impart excellent front-mid flavor to food, especially Propionibacterium antimicrobial peptides that have kokumi properties, and existing preparation technologies are unable to combine Asp and Glu to form γ-Glu-ASP.

Method used

Asp and Glu are added to a propionibacterium culture medium, and a propionibacterium metabolite containing γ-Glu-ASP is prepared by fermentation. The obtained propionibacterium cells are used to prepare a propionibacterium metabolite containing γ-Glu-ASP.

Benefits of technology

The prepared γ-Glu-ASP Propionibacterium metabolite has a significant kokumi effect, especially the front and middle taste, and the antibacterial activity is increased by about 50%, and can be used in condiments and food additives.

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Abstract

The invention discloses a propionibacterium metabolite containing gamma-Glu-ASP and a preparation method of the propionibacterium metabolite, and belongs to the technical field of food seasonings, the propionibacterium metabolite contains 0.3% or more of gamma-Glu-ASP per unit dry weight, according to the propionibacterium metabolite containing the gamma-Glu-ASP, propionibacterium is cultured by adopting a culture medium added with two compounds of Asp and Glu, and the propionibacterium metabolite containing the gamma-Glu-ASP is prepared by utilizing the obtained propionibacterium thallus. The bacteriostatic activity of the propionibacterium metabolite containing gamma-Glu-ASP disclosed by the invention is about 50% higher than that of a conventional propionibacterium metabolite without Glu and Asp; the propionibacterium metabolite containing gamma-Glu-ASP endows food with excellent strong taste, especially has a remarkable effect on the first taste and the second taste, and can be used in the industries of seasonings or food additives and the like.
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