A uorf element for regulating expression of chicken insulin receptor and application thereof

By constructing uORF elements and their recombinant vectors that regulate the expression of the chicken INSR gene, the problem of chicken insulin resistance was solved, the expression of the chicken insulin receptor was regulated, and insulin sensitivity was improved, providing new materials for the study of insulin regulation in chickens and humans.

CN120796258BActive Publication Date: 2026-07-24NORTHEAST AGRICULTURAL UNIVERSITY
View PDF 1 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NORTHEAST AGRICULTURAL UNIVERSITY
Filing Date
2025-05-26
Publication Date
2026-07-24

AI Technical Summary

Technical Problem

There are currently no reports on the regulation of chicken insulin receptor (INSR) gene expression by uORF elements. Chickens have insulin resistance, which affects their growth, development and metabolism, and there is a lack of effective regulatory means.

Method used

A uORF element and its PCR primer set for regulating chicken INSR gene expression were designed and constructed. The uORF element was expressed in eukaryotic cells using a recombinant vector. The upstream uORF element of the INSR gene was regulated using the psi-CHECK2 vector, including the construction of a reporter gene vector with start codon mutation, for regulating chicken insulin receptor expression.

Benefits of technology

It significantly increased the expression level of the chicken insulin receptor and improved insulin sensitivity, providing research support for elucidating the mechanism of insulin regulation of blood glucose, and has important application value.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure CN120796258B_ABST
    Figure CN120796258B_ABST
Patent Text Reader

Abstract

The application discloses a uORF element for regulating chicken insulin receptor expression and application thereof, belongs to the field of molecular biology and genetic engineering, and the nucleotide sequence of the uORF element is shown in any one of SEQ ID NO. 1-2; the application comprises any one of the following: (1) regulating the expression of firefly luciferase in eukaryotic cells; the eukaryotic cells are chicken embryo fibroblasts and chicken preadipocytes; (2) being applied to genetic breeding of chickens, and the genetic breeding is breeding of meat chickens with reduced insulin resistance; (3) application in preparation of a reagent for regulating the expression of chicken insulin receptors. The application can regulate the expression level of INSR, improve insulin sensitivity, and provide material support for research on the mechanism of insulin in regulating blood glucose.
Need to check novelty before this filing date? Find Prior Art