Method for green extraction of waste beer yeast cell wall polysaccharide by using eutectic solvent

By selectively dissolving and separating mannan in a eutectic solvent, combined with enzymatic purification and solvent recovery, the problem of comprehensive utilization of polysaccharide components in waste brewer's yeast has been solved, achieving efficient, green, and low-cost polysaccharide extraction and recovery.

CN120865451APending Publication Date: 2025-10-31CHINA AGRI UNIV
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Patent Information

Application Number
CN202511299857.2
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-09-12
Publication Date
2025-10-31

AI Technical Summary

Technical Problem

Existing technologies for utilizing waste brewer's yeast suffer from problems such as insufficient resource utilization, environmental pollution, and high costs, especially the failure to effectively utilize β-glucan and mannan in the yeast cell wall.

Method used

Pretreatment with a eutectic solvent selectively dissolves and recovers mannan. β-glucan is extracted through solid-liquid separation and enzymatic purification. Mannan is then precipitated using an antisolvent, and the eutectic solvent is recovered, achieving synergistic extraction of polysaccharide components and recycling of the solvent.

Benefits of technology

This technology enables the efficient separation and recovery of two major polysaccharide components from the cell walls of waste brewer's yeast, reducing production costs, improving resource utilization and environmental friendliness, and significantly enhancing the economic feasibility and output value of the process.

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Abstract

The invention provides a method for green extraction of waste beer yeast cell wall polysaccharide by using a deep eutectic solvent, relates to the field of biomass resource utilization, and aims to solve the problems of environmental pollution, high cost and insufficient resource utilization in the prior art. The method comprises the following steps: pretreating a yeast raw material and a deep eutectic solvent to selectively dissolve mannan; performing solid-liquid separation for the first time to obtain insoluble beta-glucan and a first supernatant containing mannan; an anti-solvent is added into the supernate for precipitation, and mannan is obtained through secondary solid-liquid separation; and finally, treating the separated liquid to recover the eutectic solvent. The two polysaccharides are cooperatively extracted, the solvent is recycled, comprehensive utilization of resources is achieved, and the process is environmentally friendly, low in cost and high in efficiency.
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