A hair loss prevention composition containing burdock root and a preparation method and application thereof

Abstract

The present application belongs to the technical field of daily chemicals, and particularly relates to a hair loss prevention composition containing burdock roots and a preparation method and application thereof. The hair loss prevention composition containing burdock roots is prepared from the following raw materials in parts by weight: 0.1-2 parts of burdock root extract, 0.1-2 parts of cypress leaf extract, 0.1-2 parts of Chinese aristolochia root extract, 0.1-2 parts of angelica root extract and 0.1-2 parts of honeysuckle flower extract. The composition is prepared from the components such as burdock root extract, cypress leaf extract, Chinese aristolochia root extract, angelica root extract and honeysuckle flower extract, can effectively prevent hair loss and inhibit inflammation, and prevent the generation of inflammation of the scalp.

Description

A hair loss prevention composition containing burdock root, its preparation method and application Technical Field

[0001] This invention falls under the category of daily chemical product technology, specifically relating to an anti-hair loss composition containing burdock root, its preparation method, and its application. Background Technology

[0002] Anti-hair loss formulas are a class of compound formulations used to prevent hair loss and improve hair growth. They are typically composed of multiple active ingredients that work through multi-target effects, including inhibiting hair loss, promoting hair follicle health, and improving the scalp environment. Their ingredients need to target common causes of hair loss (such as androgen sensitivity, hair follicle atrophy, scalp inflammation, and nutritional deficiencies) and can be widely used in shampoos, hair growth serums, serums, and other hair care or functional hair products.

[0003] Burdock root is rich in polyphenols, flavonoids, and vitamins (such as vitamins C and E), possessing strong antioxidant capabilities. It can eliminate free radicals on the scalp and reduce oxidative stress damage to hair follicles (oxidative stress is one of the factors that accelerate hair follicle aging and lead to hair loss). Simultaneously, its anti-inflammatory activity helps relieve scalp inflammation (such as redness and itching caused by seborrheic dermatitis), preventing long-term inflammation from stimulating hair follicle degeneration. Furthermore, burdock root contains various amino acids and minerals (such as calcium, iron, and zinc), providing nutritional support to hair follicles and improving the foundation for hair growth. Traditional Chinese medicine believes that burdock root can "unblock meridians and activate collaterals," and modern research also suggests that it may help activate dormant hair follicles by promoting local blood circulation in the scalp and increasing blood supply to hair follicles. Moreover, burdock root extract has certain oil-controlling and antibacterial effects, reducing excessive sebum secretion on the scalp (sebum clogging hair follicles easily leads to hair loss) and inhibiting the growth of fungi such as Malassezia, helping to maintain a clean and healthy scalp microenvironment and indirectly reducing the causes of hair loss. Burdock root is often added in the form of extracts to topical products such as anti-hair loss shampoos and hair growth serums, where it works synergistically with other ingredients.

[0004] Chinese patent CN113940904A discloses a traditional Chinese medicine shampoo for dandruff removal and hair loss prevention, belonging to the technical field of daily chemical products. This invention is prepared from the following raw materials in parts by weight: 75-95 parts shampoo base, and 5-15 parts extract of a traditional Chinese medicine composition for dandruff removal and hair loss prevention; wherein the extract consists of 6-15 parts of Paeonia lactiflora, 10-20 parts of Arctium lappa root, 3-8 parts of Astragalus membranaceus, 3-8 parts of Trigonella foenum-graecum leaf, and 3-8 parts of Hippophae rhamnoides.

[0005] Invention patent CN1332646C discloses a shampoo for treating gray hair and hair loss and detoxifying, which is composed of Sophora flavescens, Angelica dahurica, Arctium lappa, Saposhnikovia divaricata, Aconitum carmichaelii, AES, water, etc., and is made through steps such as weighing, decocting, filtering, and stirring.

[0006] Invention patent CN116251047B discloses an oil-controlling shampoo containing burdock root extract and its preparation method. The oil-controlling shampoo comprises the following raw materials: plant extract components, hair conditioning agents, surfactants, thickeners, functional additives, and purified water as a solvent; wherein: the plant extract components include the following raw materials: burdock root extract, artemisia annua extract, purslane extract, dandelion extract, rhubarb extract, arborvitae leaf extract, cedarwood essential oil, glycyrrhizic acid, and ferrous sulfate extract.

[0007] Invention patent CN113679637B discloses a burdock root ferment, products containing the same, their preparation methods, and applications. The preparation method for the burdock root ferment is either Method I or Method II. Method I includes the following steps: inoculating a fermentation substrate with *Hericium erinaceus* (accession number CGMCCNo.3.15191), performing solid-state fermentation for 7-14 days, sterilizing, and obtaining a crude burdock root ferment; wherein the fermentation substrate includes burdock root. Method II includes the following steps: subjecting the crude burdock root ferment obtained in Method I to water extraction to obtain a fermented water extract of burdock root.

[0008] While burdock root extract and other ingredients have certain anti-hair loss effects, enhancing the anti-hair loss effect of single burdock root extracts and improving the overall anti-hair loss effect of compositions containing burdock root are still technical problems that need to be solved. Summary of the Invention

[0009] To address the aforementioned technical problems, this invention proposes an anti-hair loss composition containing burdock root, its preparation method, and its application.

[0010] In one aspect, the present invention provides a hair loss prevention composition containing burdock root, wherein the raw materials are composed of: 0.1-2 parts burdock root extract, 0.1-2 parts arborvitae leaf extract, 0.1-2 parts eclipta prostrata extract, 0.1-2 parts angelica sinensis root extract and 0.1-2 parts honeysuckle flower extract by weight.

[0011] Preferably, the above composition further comprises, by weight, 0.1-2 parts of hop flower extract and 0.1-2 parts of short-stalked wild sesame flower extract.

[0012] Preferably, in the above composition, the weight ratio of hop flower extract and short-stalked wild sesame flower extract is 1:0.5-1.5.

[0013] Preferably, the extracts of Platycladus orientalis leaves, Eclipta prostrata, Angelica sinensis root, and Lonicera japonica flowers are obtained by heating, filtering, concentrating, and drying with a 40-60 wt% ethanol solution. The weight ratio of plant material to ethanol solution in the extracts is 1:10-100. The heating and extraction temperature and time are 40-60℃ for 2-10 hours. Concentration is performed under reduced pressure. Drying can be performed using vacuum drying or freeze drying.

[0014] Preferably, a more specific example of the preparation method of the above-mentioned Platycladus orientalis leaf extract, Eclipta prostrata extract, Angelica sinensis root extract and Lonicera japonica flower extract is as follows: taking Platycladus orientalis leaf extract as an example, the preparation method includes: taking dried Platycladus orientalis leaves, pulverizing them to less than 50 mesh, adding 10-100 times the weight of the powder in a 40-60 wt% ethanol solution, heating to 40-60℃ and stirring for 2-10 hours, cooling, filtering, concentrating under reduced pressure, and drying to obtain the extract.

[0015] Preferably, both hop flower extract and short-stalked wild sesame flower extract are obtained by heating, filtering, concentrating, and drying with a 25-45 wt% ethanol solution. In the above preparation, the weight ratio of plant material to ethanol solution is 1:10-100. The heating and extraction temperature and time are 40-60℃ for 2-10 hours. Concentration is performed under reduced pressure. Drying can be achieved through vacuum drying or freeze drying.

[0016] Preferably, a more specific example of the preparation method of the above-mentioned hop flower extract and short-stalked wild sesame flower extract is as follows: taking the hop flower extract as an example, the preparation method includes: taking dried hop flowers, pulverizing them to less than 50 mesh, adding 10-100 times the weight of the powder in a 25-45wt% ethanol solution, heating to 40-60℃ and stirring for 2-10 hours, cooling, filtering, concentrating, and drying to obtain the extract.

[0017] Preferably, the preparation method of burdock root extract includes: taking dried burdock root, pulverizing it, adding water, heating and stirring, cooling to obtain the material, adding sucrose and fermentation bacteria, fermenting, sterilizing, filtering, concentrating, and drying to obtain burdock root extract. The weight ratio of burdock root powder to water is 1:5-50. The amount of sucrose added is 0.5-3% of the material weight. The fermentation bacteria are Aspergillus oryzae CICC 2359 and Bacillus subtilis GDMCC NO.1.1370 in a quantity ratio of 1:1-2. The total amount of fermentation bacteria added is 10. 9 -10 12 1 cell / g of material. Fermentation temperature and time: 25-40℃, 2-5 days. Concentration is performed under reduced pressure. Drying can be performed using vacuum drying or freeze drying.

[0018] Preferably, the preparation method of burdock root extract includes: taking dried burdock root, pulverizing it to less than 50 mesh, adding water at a weight ratio of 1:5-50 and heating to 60-80℃ while stirring for 0.5-5 hours, cooling to obtain the material, adding 0.5-3% by weight of sucrose and fermentation bacteria (the fermentation bacteria are Aspergillus oryzae CICC 2359 and Bacillus subtilis GDMCC NO.1.1370 in a quantity ratio of 1:1-2, and the total amount of fermentation bacteria added is 10g) 9 -10 12 (1 bacteria / g of material), then ferment at 25-40℃ for 2-5 days, then sterilize, filter, concentrate, and dry to obtain burdock root extract.

[0019] The present invention also provides a method for preparing the hair loss prevention composition containing burdock root, comprising the processes of mixing raw materials, packaging and sterilizing.

[0020] This invention also provides the use of the composition in the preparation of cosmetics. The cosmetic has anti-hair loss and anti-inflammatory functions, and is a shampoo preparation. The content of the composition in the cosmetic is 0.01-10 wt%. Cosmetics also contain at least one of the following: water, sodium lauryl ether sulfate, glycerin, cocamidopropyl betaine, ammonium lauryl sulfate, pearlescent flakes, sodium methyl cocoyl taurate, acrylate polymers, emulsified silicone oil, polyquaternium-7, polyquaternium-6, polyquaternium-22, cocamide MEA, polydimethylsiloxane, pyrrolidone ethanolamine salt, fragrance, Coptis chinensis root extract, Scutellaria baicalensis extract, Rheum palmatum extract, Ligusticum chuanxiong extract, sodium chloride, cetearyl alcohol, allantoin, Artemisia annua extract, Portulaca oleracea extract, menthol, guar gum hydroxypropyltrimethylammonium chloride, EDTA-2Na, plant glycoproteins, dandelion extract, saccharide isomers, preservatives, laurocapram, and hexamidine di(hydroxyethyl sulfonic acid) salt.

[0021] The present invention also provides a cosmetic comprising the above-mentioned composition, specifically a shampoo formulation, wherein, by weight percentage, the cosmetic comprises: 0.01-10% composition, 6-18% sodium lauryl ether sulfate, 1-12% glycerin, 1-6% cocamidopropyl betaine, 0.5-6% ammonium lauryl sulfate, 0.5-6% pearlescent flakes, 0.4-3% sodium methyl cocoyl taurate, 0.3-1.8% acrylate polymer, 0.5-3% emulsified silicone oil, 0.2-7% polyquaternium-7, 0.01-3.5% polyquaternium-6, 0.02-3.5% polyquaternium-22, 0.4-2.5% cocamide MEA, 0.02-1.8% polydimethylsiloxane, 0.2-0.8% piroctone ketone ethanolamine salt, 0.2-1.1% fragrance, 0. 0.005-1% Coptis chinensis root extract, 0.005-1% Scutellaria baicalensis extract, 0.005-1% Rheum palmatum extract, 0.005-1% Ligusticum chuanxiong extract, 0.1-2% sodium chloride, 0.1-0.5% cetearyl alcohol, 0.1-0.5% allantoin, 0.005-1% Artemisia annua extract, 0.005-1% Portulaca oleracea extract, 0.1-0.5% menthol, 0.1-0 0.2% Guar Gum Hydroxypropyl Trimethylammonium Chloride, 0.01-0.5% EDTA-2Na, 0.05-0.5% Plant Glycoprotein, 0.005-1% Dandelion Extract, 0.05-0.5% Carbohydrate Isomers, 0.005-0.2% Preservatives, 0.01-0.1% Lauryl Azone, 0.001-0.02% Hexamididine Di(Hydroxyethyl Sulfonic Acid) Salt and Balance Water.

[0022] The advantages of this invention compared to the prior art are as follows:

[0023] The burdock root extract prepared by this invention can effectively promote hair loss prevention and hair growth.

[0024] In preparing burdock root extract, this invention selects fermented burdock root containing Aspergillus oryzae CICC 2359 and Bacillus subtilis GDMCC NO.1.1370, which can effectively enhance the effect of preventing hair loss and promoting hair growth. The fermentation process of the above-mentioned bacteria promotes the richness and variety of active ingredients, which is beneficial to improving the effect of preventing hair loss and promoting hair growth.

[0025] This invention uses *Aspergillus oryzae* CICC 2359 and *Bacillus subtilis* GDMCC NO.1.1370 as fermentation bacteria, which can further enhance the efficacy of the extract compared to other bacteria. Furthermore, when *Aspergillus oryzae* CICC 2359 and *Bacillus subtilis* GDMCC NO.1.1370 are used for co-fermentation, the effect is better than that of a single bacterium. The co-fermentation of the two bacteria produces a synergistic effect, mutually assisting and promoting fermentation, significantly enhancing the anti-hair loss and hair regrowth effect. Especially when the ratio of the two bacteria is in the range of 1:1-2, the anti-hair loss and hair regrowth effect is even better.

[0026] The hop flower extract and short-stalked wild sesame flower extract prepared by this invention can effectively inhibit the production of inflammatory factors and have good anti-inflammatory effects.

[0027] The hop flower extract and short-stalked wild sesame flower extract prepared by this invention can produce a synergistic effect in a ratio of 1:0.2-8. The active ingredients of the two complement each other, enriching the types and concentrations of anti-inflammatory active ingredients, thereby further enhancing the anti-inflammatory effect. Moreover, the combination of the two produces the best anti-inflammatory effect when the ratio is in the range of 1:0.5-1.5.

[0028] This invention uses burdock root extract, arborvitae leaf extract, eclipta prostrata extract, angelica root extract and honeysuckle flower extract to prepare a composition. The composition can effectively prevent hair loss and promote hair growth. In addition, it can effectively inhibit inflammation and prevent the occurrence of scalp inflammation. Attached Figure Description

[0029] Figure 1: Hair follicle condition of volunteer A's scalp before and after using the preparation.

[0030] Figure 2: Hair follicle condition of volunteer B's scalp before and after using the preparation.

[0031] Figure 3: Hair follicle condition of volunteer C before and after using the preparation. Detailed Implementation

[0032] Example 1

[0033] The effects of different preparation processes of burdock root extract on the anti-hair loss properties of the extract were tested.

[0034] Burdock root extract I:

[0035] Take dried burdock (ARCTIUM LAPPA) roots, pulverize them to below 50 mesh, add water at a weight ratio of 1:30, heat to 70℃, stir at 70 rpm for 1 hour, cool to room temperature to obtain the material, then add 1.85% sucrose and fermentation bacteria (the fermentation bacteria consist of Aspergillus oryzae CICC 2359 and Bacillus subtilis GDMCC NO.1.1370 in a 1:1 ratio, with a total fermentation bacteria content of 2.64 × 10⁻⁶). 11 The burdock root extract was prepared by fermenting the material at 35°C with aerobic fermentation for 3 days (sterile air flow rate 0.65 vvm, stirring at 70 rpm), then sterilizing, filtering with a 0.22 μm pore size filter membrane, concentrating the filtrate under reduced pressure to 15.3% of the filtrate weight, and then freeze-drying to a water content of 1.64 wt% to obtain burdock root extract I.

[0036] Burdock Root Extract II:

[0037] Take dried burdock (ARCTIUM LAPPA) roots, pulverize them to below 50 mesh, add water at a weight ratio of 1:28, heat to 68℃, stir at 70 rpm for 1 hour, cool to room temperature to obtain the material, then add 1.93% sucrose and fermentation bacteria (the fermentation bacteria consist of Aspergillus oryzae CICC 2359 and Bacillus subtilis GDMCC NO.1.1370 in a quantity ratio of 1:2, with a total fermentation bacteria content of 2.16 × 10⁻⁶). 11 The sample was fermented at 32°C for 3.5 days with aerobic fermentation (sterile air flow rate 0.60 vvm, stirring at 70 rpm), then sterilized, filtered through a 0.22 μm pore size filter membrane, and the filtrate was concentrated under reduced pressure to 14.6% of the filtrate weight, and then freeze-dried to a water content of 1.72 wt% to obtain burdock root extract II.

[0038] Burdock Root Extract III: The difference between this and Burdock Root Extract I is that it does not undergo fermentation. The specific preparation method is as follows:

[0039] Take dried burdock (ARCTIUM LAPPA) root, pulverize it to less than 50 mesh, add water at a weight ratio of 1:30 and heat to 70℃, stir at 70 rpm for 1 hour, cool to room temperature to obtain the material, filter it through a 0.22 μm pore size filter membrane, concentrate the obtained filtrate under reduced pressure to 15.3% of the filtrate weight, and then freeze-dry to a water content of 1.64 wt% to obtain burdock root extract III.

[0040] Burdock Root Extract IV: The difference between this and Burdock Root Extract I is that one type of bacteria is used in the fermentation process, while the amount of bacteria remains the same. The specific preparation method is as follows:

[0041] Take dried burdock root, pulverize it to less than 50 mesh, add water at a weight ratio of 1:30, heat to 70℃ and stir at 70 rpm for 1 hour, cool to room temperature to obtain the material, then add 1.85% sucrose and fermentation bacteria (Aspergillus oryzae CICC 2359, the amount of fermentation bacteria added is 2.64 × 10⁻⁶) by weight of the material. 11 The burdock root extract (BAR) was then fermented at 35°C for 3 days with aerobic fermentation (sterile air flow rate 0.65 vvm, stirring at 70 rpm), sterilized, filtered through a 0.22 μm pore size membrane, and the resulting filtrate was concentrated under reduced pressure to 15.3% by weight, and then freeze-dried to a water content of 1.64 wt% to obtain burdock root extract IV.

[0042] Burdock Root Extract V: The difference between this and Burdock Root Extract I is that one type of bacteria is used in the fermentation process, while the amount of bacteria remains the same. The specific preparation method is as follows:

[0043] Take dried burdock root, pulverize it to less than 50 mesh, add water at a weight ratio of 1:30, heat to 70℃ and stir at 70 rpm for 1 hour, cool to room temperature to obtain the material, then add 1.85% sucrose and fermentation bacteria (Bacillus subtilis, 2.64 × 10⁻⁶) by weight of the material. 11 The bacteria were divided into 100 cells / g of material, and then fermented aerobically at 35°C for 3 days (sterile air flow rate 0.65vvm, stirring at 70rpm). Then sterilized, filtered through a 0.22μm pore size filter membrane, the filtrate was concentrated under reduced pressure to 15.3% of the filtrate weight, and then freeze-dried to a water content of 1.64wt% to obtain burdock root extract V.

[0044] Burdock Root Extract VI: The difference between this and Burdock Root Extract I is that different bacteria are used in the fermentation process, while the amount of bacteria remains the same. The specific preparation method is as follows:

[0045] Take dried burdock (ARCTIUM LAPPA) roots, pulverize them to below 50 mesh, add water at a weight ratio of 1:30, heat to 70℃, stir at 70 rpm for 1 hour, cool to room temperature to obtain the material, then add 1.85% sucrose and fermentation bacteria (Bacillus licheniformis CICC 10095, total amount of fermentation bacteria added: 2.64 × 10⁻⁶) by weight of the material. 11 The bacteria count was 1 / g of material, and then fermented aerobically at 35°C for 3 days (sterile air flow rate 0.65vvm, stirring at 70rpm). After sterilization, the mixture was filtered through a 0.22μm pore size filter membrane. The filtrate was concentrated under reduced pressure to 15.3% of the filtrate weight, and then freeze-dried to a water content of 1.64wt% to obtain burdock root extract VI.

[0046] Burdock Root Extract VII: The difference between this and Burdock Root Extract I lies in the change in the proportion of fermentation bacteria, while the amount of bacteria remains the same. The specific preparation method is as follows:

[0047] Take dried burdock root, pulverize it to less than 50 mesh, add water at a weight ratio of 1:30, heat to 70℃ and stir at 70 rpm for 1 hour, cool to room temperature to obtain the material, then add 1.85% sucrose and fermentation bacteria (the fermentation bacteria consist of Aspergillus oryzae CICC 2359 and Bacillus subtilis GDMCC NO.1.1370 in a quantity ratio of 1:0.2, with a total fermentation bacteria content of 2.64 × 10⁻⁶). 11 The burdock root extract VII was obtained by fermenting the material at 35°C with aerobic fermentation for 3 days (sterile air flow rate 0.65 vvm, stirring at 70 rpm), sterilizing, filtering with a 0.22 μm pore size filter membrane, concentrating the filtrate under reduced pressure to 15.3% of the filtrate weight, and then freeze-drying to a water content of 1.64 wt%.

[0048] Burdock Root Extract VIII: The difference between this and Burdock Root Extract I lies in the change in the proportion of fermentation bacteria, while the amount of bacteria remains the same. The specific preparation method is as follows:

[0049] Take dried burdock root, pulverize it to less than 50 mesh, add water at a weight ratio of 1:30, heat to 70℃ and stir at 70 rpm for 1 hour, cool to room temperature to obtain the material, then add 1.85% sucrose and fermentation bacteria (the fermentation bacteria consist of Aspergillus oryzae CICC 2359 and Bacillus subtilis GDMCC NO.1.1370 in a quantity ratio of 1:8, with a total fermentation bacteria content of 2.64 × 10⁻⁶). 11 The bacteria count was 1 / g of material, and then fermented aerobically at 35°C for 3 days (sterile air flow rate 0.65vvm, stirring at 70rpm). After sterilization, the mixture was filtered through a 0.22μm pore size filter membrane. The filtrate was concentrated under reduced pressure to 15.3% of the filtrate weight, and then freeze-dried to a water content of 1.64wt% to obtain burdock root extract VIII.

[0050] The hair loss prevention effect of the burdock root extract prepared above was tested using the following specific testing methods:

[0051] Several male C57BL / 6 mice (20±2g each) were collected from the same batch. After one week of acclimatization, a 2cm×2cm area on the same part of the mouse's back was shaved as the test area. Mice with no skin damage after hair removal were randomly divided into two groups according to their weight: a control group and a burdock root extract I-VIII group, with 8 mice in each group. Mice in the burdock root extract I-VIII group had a 2% aqueous solution of the extract applied to the test area at a rate of 0.1g / day per mouse (administered at 8:00 AM and 6:00 PM, 0.05g each time). The control group received the same amount of water in the same manner. The administration continued for 6 weeks. At weeks 4 and 6, 10 hairs were randomly selected from the test area of ​​each group to measure the length, and the results were statistically analyzed. The results are shown in Table 1. In Table 1, the p-values ​​of each group compared to the control group were <0.05.

[0052] Table 1: Hair Length

[0053]

[0054] Subtract the average hair length of each group from the control group in Table 1 to obtain the hair length Δ value of each group compared with the control group. The results are shown in Table 2.

[0055] Table 2: Hair length growth Δ value compared to the control group

[0056]

[0057] According to the tests in Table 1-2 above, the burdock root extract prepared by this invention can effectively promote hair loss prevention and hair growth.

[0058] A comparison of extracts I and III shows that the fermentation of burdock root using *Aspergillus oryzae* CICC 2359 and *Bacillus subtilis* GDMCC NO.1.1370 effectively enhances the hair loss prevention and hair regrowth effects. The fermentation process of these bacteria promotes the richness and variety of active ingredients, which is beneficial to improving the hair loss prevention and hair regrowth effects. A comparison of extracts I and VI shows that the use of *Aspergillus oryzae* CICC 2359 and *Bacillus subtilis* GDMCC NO.1.1370 as fermentation bacteria in this invention further enhances the extract's effect compared to other bacteria. Furthermore, the combined fermentation of *Aspergillus oryzae* CICC 2359 and *Bacillus subtilis* GDMCC NO.1.1370 is more effective than using a single bacteria. This finding, combined with the comparison of extracts I, IV, and V, indicates that the combined fermentation of these two bacteria produces a synergistic effect, mutually assisting and promoting fermentation, significantly enhancing the hair loss prevention and hair regrowth effects. Especially when the ratio of the two is in the range of 1:1-2, the hair loss prevention and hair growth effect is better, which can be obtained by combining the comparison of extracts I, II, VII and VIII.

[0059] Example 2

[0060] This embodiment specifically studies the inhibitory effect of the combination of hop flower extract (HUMULUS LUPULUS) and strophanthidin flower extract (LAMIUMALBUM) on skin inflammation.

[0061] The preparation method of hop (HUMULUS LUPULUS) flower extract includes: taking dried hop flowers, pulverizing them to less than 50 mesh, adding 20 times the weight of the powder in a 35wt% ethanol solution, heating to 55℃ and keeping warm while stirring at 70rpm for 4 hours, cooling to room temperature, filtering through a 0.22μm pore size filter membrane, concentrating the filtrate under reduced pressure to 14.3% of the filtrate weight, and then freeze-drying to a water content of 1.76wt% to obtain the extract.

[0062] The preparation method of the extract of *Lamium album* flower includes: taking dried *Lamium album* flowers, pulverizing them to less than 50 mesh, adding 25 times the weight of the powder in 30wt% ethanol solution, heating to 57℃ and keeping warm while stirring at 75rpm for 4.5 hours, cooling to room temperature, filtering through a 0.22μm pore size filter membrane, concentrating the filtrate under reduced pressure to 15.7% of the filtrate weight, and freeze-drying to a water content of 1.83wt% to obtain the extract.

[0063] Test samples were prepared according to the combinations in Table 3, and the anti-inflammatory effects were tested.

[0064] Table 3: Composition of Test Samples

[0065]

[0066] The samples in Table 3 were subjected to anti-inflammatory tests. The specific test steps were as follows: RAW264.7 cells in the logarithmic growth phase were used at a concentration of 1.5 × 10⁻⁶ cells / mL. 5 Cells were seeded at a density of [number] cells / mL into 24-well plates, with 0.5 mL of DEME medium added to each well. The plates were incubated at 37°C and 5% CO2 in the dark for 24 hours. Cells were then divided into a control group, a model group, and a sample IX group, with five replicates per group. In the model group, DEME medium was replaced and LPS was added to a concentration of 1 μg / mL, with a total volume of 0.5 mL per well. In the sample IX group, DEME medium was replaced and LPS was added to a concentration of 1 μg / mL, along with the corresponding sample to a concentration of 45 μg / mL, with a total volume of 0.5 mL per well. In the control group, DEME medium was replaced, with a total volume of 0.5 mL per well. All cells were incubated at 37°C and 5% CO2 in the dark for another 24 hours. The supernatant was then collected, and the concentrations of IL-6 and TNF-α were measured according to the ELISA kit instructions. The results are shown in Table 4. In Table 4, the p-values ​​of all groups compared to the control group and the model group were all <0.05.

[0067] Table 4: IL-6 and TNF-α concentrations

[0068]

[0069] As shown in Table 4, after establishing the validation model using LPS, the concentrations of TNF-α and IL-6 in the model group were significantly increased compared to the blank group. Sample IX (hop flower extract and / or short-stalked wild sesame flower extract) could effectively inhibit the production of inflammatory factors IL-6 and TNF-α, and had a good anti-inflammatory effect.

[0070] While maintaining a consistent total dosage, the anti-inflammatory effects of samples I and II, which contained either hop flower extract or short-stalked sesame flower extract alone, were lower than those of samples IV-X, which contained both. According to the test results, hop flower extract and short-stalked sesame flower extract can produce a synergistic effect in a ratio of 1:0.2-8. The active ingredients of the two complement each other, enriching the types and concentrations of anti-inflammatory active ingredients, thus further enhancing the anti-inflammatory effect. Moreover, the combination of the two produces the best anti-inflammatory effect when the ratio is in the range of 1:0.5-1.5.

[0071] Example 3

[0072] Prepare the composition according to the raw materials and amounts (parts by weight) in Table 5.

[0073] Table 5: Composition of the composition

[0074]

[0075] In Table 5 above, the burdock root extract is the burdock root extract I prepared in Example 1, and the hop flower extract and the short-stalked wild sesame flower extract are prepared in Example 2.

[0076] In addition, other raw material preparation methods are as follows:

[0077] Oriental arborvitae (BIOTA ORIENTALIS) leaf extract: Dried oriental arborvitae leaves were pulverized to less than 50 mesh, and 30 times the weight of the powder in a 55wt% ethanol solution was added. The mixture was heated to 52℃ and kept warm while stirring at 60rpm for 4 hours. After cooling to room temperature, the mixture was filtered through a 0.22μm pore size filter membrane. The filtrate was concentrated under reduced pressure to 13.2% of its weight and freeze-dried to a water content of 1.90wt% to obtain the extract.

[0078] Eclipta prostrata extract: The dried aerial parts of Eclipta prostrata were pulverized to less than 50 mesh, and 28 times the weight of the powder in 50wt% ethanol solution was added. The mixture was heated to 50℃ and kept at that temperature while stirring at 60rpm for 4.5 hours. After cooling to room temperature, the mixture was filtered through a 0.22μm pore size filter membrane. The filtrate was concentrated under reduced pressure to 12.5% ​​by weight and then freeze-dried to a water content of 2.17wt% to obtain the extract.

[0079] Angelica POLYMORPHA SINENSIS root extract: Take dried Angelica POLYMORPHA root, pulverize to less than 50 mesh, add 27 times the weight of the powder in 37wt% ethanol solution, heat to 48℃ and keep warm while stirring at 65rpm for 5 hours, cool to room temperature, filter through a 0.22μm pore size filter membrane, concentrate the filtrate under reduced pressure to 13.3% of the filtrate weight, freeze-dry to a water content of 2.24wt%, and obtain the extract.

[0080] Honeysuckle (LONICERA JAPONICA) flower extract: Dried honeysuckle flowers were pulverized to a fineness below 50 mesh, and a 25 wt% ethanol solution (25 times the weight of the powder) was added. The mixture was heated to 50°C and kept warm while stirring at 65 rpm for 5.5 hours. After cooling to room temperature, the mixture was filtered through a 0.22 μm pore size filter membrane. The filtrate was concentrated under reduced pressure to 13.7% of its weight and freeze-dried to a water content of 2.03 wt% to obtain the extract.

[0081] The preparation method of the above compositions 1-5 is as follows: the raw materials are stirred and mixed in a container, then packaged and sterilized according to specifications to obtain the compositions.

[0082] Example 4

[0083] The effects of the above-prepared combination were tested. Specifically, 10 male volunteers suffering from hair loss were recruited and used the preparation (composed of composition 1 and solvent in a weight ratio of 1:20, wherein the solvent is composed of glycerin and deionized water in a weight ratio of 1:3) twice a day, 1.2 mL each time, on the hair loss area, for three consecutive months.

[0084] Before and after using the formulation, the scalp follicles of volunteers were observed under a 50x microscope using an octa-core quad-spectrum follicle / scalp / skin analyzer (11-inch, model: M-12). The test showed that the hair concentration of all 10 volunteers increased to some extent.

[0085] The accompanying Figures 1-3 show the scalp condition of three volunteers (volunteers A, B, and C) before and after use. In Figures 1-3, the left image shows the scalp and hair follicle condition before using the formulation, and the right image shows the scalp and hair follicle condition after three months of using the formulation. Therefore, it can be seen that the composition prepared by this invention can effectively prevent hair loss and promote hair growth.

Claims

1. A hair loss prevention composition containing burdock root, characterized in that, The raw materials, by weight, consist of: 0.1-2 parts burdock root extract, 0.1-2 parts arborvitae leaf extract, 0.1-2 parts eclipta prostrata extract, 0.1-2 parts angelica root extract, 0.1-2 parts honeysuckle flower extract, 0.1-2 parts hop flower extract, and 0.1-2 parts short-stalked wild sesame flower extract; the weight ratio of hop flower extract to short-stalked wild sesame flower extract is 1:0.5-1.5; both hop flower extract and short-stalked wild sesame flower extract are obtained by heating, filtering, concentrating, and drying with 25-45 wt% ethanol solution.

2. The anti-hair loss composition containing burdock root according to claim 1, characterized in that, The extracts of Platycladus orientalis leaves, Eclipta prostrata, Angelica sinensis root, and Lonicera japonica were obtained by heating, filtering, concentrating, and drying in a 40-60 wt% ethanol solution.

3. A hair loss prevention composition containing burdock root according to any one of claims 1-2, characterized in that, The preparation method of burdock root extract includes: taking dried burdock root, crushing it, adding water, heating and stirring, cooling to obtain the material, adding sucrose and fermentation bacteria, fermenting, sterilizing, filtering, concentrating, and drying to obtain burdock root extract; the fermentation bacteria are Aspergillus oryzae CICC 2359 and Bacillus subtilis GDMCC NO.1.1370 in a quantity ratio of 1:1-2.

4. A method for preparing a hair loss prevention composition containing burdock root according to any one of claims 1-3, characterized in that, This includes the processes of mixing, packaging, and sterilizing raw materials.

5. The use of a hair loss prevention composition containing burdock root according to any one of claims 1-3 in the preparation of cosmetics, characterized in that, The cosmetic product has anti-hair loss and anti-inflammatory functions, and it is a shampoo preparation.

6. The application according to claim 5, characterized in that, The composition is present in cosmetics at a concentration of 0.01-10 wt%.

7. The application according to claim 5, characterized in that, Cosmetics may also contain at least one of the following: water, sodium lauryl ether sulfate, glycerin, cocamidopropyl betaine, ammonium lauryl sulfate, pearlescent flakes, sodium methyl cocoyl taurate, acrylate polymers, polyquaternium-7, polyquaternium-6, polyquaternium-22, cocamide MEA, polydimethylsiloxane, piroctone ketone ethanolamine salt, fragrance, Coptis chinensis root extract, Scutellaria baicalensis extract, Rheum palmatum extract, Ligusticum chuanxiong extract, sodium chloride, cetearyl alcohol, allantoin, Artemisia annua extract, Portulaca oleracea extract, menthol, guar gum hydroxypropyltrimethylammonium chloride, EDTA-2Na, plant glycoproteins, dandelion extract, saccharide isomers, preservatives, and laurocapram.

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