Anoectochilus formosanus hangover alleviating and liver protecting primary pulp and preparation method thereof

Abstract

The anoectochilus formosanus hangover alleviating and liver protecting raw stock is prepared from the following raw materials in parts by weight: 150-350 parts of anoectochilus formosanus raw juice, 100-250 parts of waxberry raw juice, 100-250 parts of Chinese wolfberry fruit raw juice, 10-30 parts of micrococcus oil, 1-5 parts of oriental cherry polyphenol and 1-5 parts of proanthocyanidin. The anoectochilus formosanus raw stock capable of dispelling effects of alcohol and protecting liver is prepared by the following method: 1) weighing anoectochilus formosanus raw juice, waxberry raw juice and Chinese wolfberry fruit raw juice according to the proportion in parts by weight, adding water which is 10-15 times of the total feeding weight, mixing, pulping, sieving and removing dregs to obtain a raw stock base, and the anoectochilus formosanus raw juice, the waxberry raw juice and the Chinese wolfberry fruit raw juice are obtained by directly pulping fresh anoectochilus formosanus raw juice, fresh waxberry fruit and fresh Chinese wolfberry fruit; the invention discloses anoectochilus formosanus protoplasm which comprises the following steps: 1) preparing a protoplasm substrate, 2) transferring the protoplasm substrate into a vacuum homogeneous emulsifying pot, slowly adding micrococcus oil at 38-42 DEG C, and homogenizing to obtain an emulsion, 3) sequentially adding oriental cherry polyphenol and proanthocyanidin under the protection of nitrogen, and homogenizing to obtain protoplasm, and 4) sterilizing the protoplasm, filling with nitrogen, and refrigerating for storage.The anoectochilus formosanus protoplasm has the effects of dispelling the effects of alcohol and protecting the liver.

Description

Technical Field

[0001] This invention belongs to the field of functional food technology, specifically relating to a *Anoectochilus roxburghii* (a type of orchid) hangover-relieving and liver-protecting syrup and its preparation method. Background Technology

[0002] Alcohol is widely used in daily etiquette and social interactions, but excessive drinking is extremely harmful to the body. It can damage organs such as the liver, gastrointestinal tract, nerves, and cardiovascular system, and can lead to diseases such as fatty liver, alcoholic hepatitis, cirrhosis, mental disorders, and stomach ulcers. Therefore, products for relieving hangovers and protecting the liver have emerged.

[0003] Golden Thread Lotus ( Anoectochilus roxburghii As a precious medicinal plant, *Anoectochilus roxburghii* enjoys a high reputation in traditional medicine and is hailed as the "King of Herbs." It is rich in various bioactive substances such as *Anoectochilus roxburghii* glycosides, polysaccharides, flavonoids, and amino acids. Modern research shows that it possesses multiple physiological functions, including antioxidant, anti-inflammatory, liver-protective, and immune-enhancing effects. Therefore, the development of deep-processed products centered on *Anoectochilus roxburghii* is increasingly favored by the market.

[0004] Currently, the main forms of Anoectochilus roxburghii products on the market include dried products, tea bags, and infused alcohol. For example, patent document (CN103493942B) discloses an Anoectochilus roxburghii tea and its preparation process, using raw materials such as Anoectochilus roxburghii, Gynostemma pentaphyllum, kidney tea, and sugar, with the product processing technology limited to drying and extraction. The production processes of these products are mostly based on traditional methods, such as hot air drying, hot water extraction, alcohol extraction, or simple pulping and coarse filtration. These methods have significant limitations: firstly, high-temperature drying or extraction processes easily destroy the heat-sensitive active ingredients in Anoectochilus roxburghii (such as Anoectochilus roxburghii glycosides, polysaccharides, and flavonoids), leading to a significant reduction in their biological efficacy; secondly, the resulting products often have a poor taste, with a noticeable herbal astringency, and the system is unstable, prone to precipitation and stratification, affecting consumer acceptance. Meanwhile, public demand for functional foods is developing towards greater complexity, higher efficiency, and more experiential aspects. In addition, many hangover relief products exist on the market, but their efficacy lacks comparative analysis and is generally low. Therefore, there is a need for complex, functional, deeply processed products to fill the market gap. Summary of the Invention

[0005] To address the lack of deep-processed products of Anoectochilus roxburghii and the low efficacy of existing hangover remedies, this invention provides an Anoectochilus roxburghii hangover remedy and liver-protecting syrup and its preparation method.

[0006] The *Anoectochilus roxburghii* hangover-relieving and liver-protecting extract of this invention is made from the following raw materials in parts by weight: 150-350 parts of *Anoectochilus roxburghii* juice, 100-250 parts of bayberry juice, 100-250 parts of wolfberry juice, 10-30 parts of *Micrococcus pluvialis* oil, 1-5 parts of cherry blossom polyphenols, and 1-5 parts of proanthocyanidins. The above-mentioned Golden Thread Orchid juice, Waxberry juice, and Goji Berry juice are obtained by directly blending fresh Golden Thread Orchid, fresh Waxberry, and fresh Goji Berry into a puree. The above-mentioned *Anoectochilus roxburghii* hangover-relieving and liver-protecting extract was prepared using the following method: 1) Weigh out the original juice of Anoectochilus roxburghii, bayberry and wolfberry according to the above weight ratio, add water 10-15 times the total weight of the ingredients, mix and pulp, sieve to remove the residue, and obtain the original pulp base; 2) Transfer the above raw slurry base to a vacuum homogenizing emulsifier, and slowly add the above-mentioned microalgae oil at 38℃-42℃ to homogenize and form a preliminary emulsion; 3) Under nitrogen protection, the above-mentioned cherry blossom polyphenols and proanthocyanidins were added sequentially and homogenized to obtain the original pulp; 4) Sterilize the above raw pulp, fill it with nitrogen in a sterile environment, and store it under cold storage.

[0007] Preferably, it is made from the following raw materials in parts by weight: 200-350 parts of Anoectochilus roxburghii juice, 150-250 parts of bayberry juice, 150-250 parts of wolfberry juice, 12-25 parts of Micrococcus pseudocarpa oil, 2-5 parts of cherry blossom polyphenols, and 2-5 parts of proanthocyanidins.

[0008] More preferably, it is made from the following raw materials in parts by weight: 250-300 parts of Anoectochilus roxburghii juice, 160-220 parts of Myrica rubra juice, 160-220 parts of Lycium barbarum juice, 18-25 parts of Micrococcus pseudocarpa oil, 3-5 parts of cherry blossom polyphenols, and 3-5 parts of proanthocyanidins.

[0009] This invention also provides a method for preparing the above-mentioned *Anoectochilus roxburghii* hangover-relieving and liver-protecting syrup, comprising the following steps: 1) Weigh out the original juice of Anoectochilus roxburghii, bayberry and wolfberry according to the above weight ratio, add water 10-15 times the total weight of the ingredients, mix and pulp, sieve to remove the residue, and obtain the original pulp base; 2) Transfer the above raw slurry base to a vacuum homogenizing emulsifier, and slowly add the above-mentioned microalgae oil at 38℃-42℃ to homogenize and form a preliminary emulsion; 3) Under nitrogen protection, the above-mentioned cherry blossom polyphenols and proanthocyanidins were added sequentially and homogenized to obtain the original pulp; 4) Sterilize the above raw pulp, fill it with nitrogen in a sterile environment, and store it under cold storage.

[0010] In step 1), a 200-300 mesh sieve is used for sieving.

[0011] In step 2), the rotation speed of the vacuum homogenizing emulsifier is 8000-12000 rpm, and the homogenization time is 10-20 minutes.

[0012] In step 3), the homogenization pressure is 1200-1500 bar, the homogenization is performed 3-4 times, and each time it lasts 8-10 minutes.

[0013] In step 4), the sterilization pressure is 400-600 MPa, the sterilization time is 3-5 minutes, and the refrigerated storage temperature is 2℃-6℃.

[0014] Beneficial effects: The *Anoectochilus roxburghii* hangover-relieving and liver-protecting extract of this invention can relieve hangovers and protect the liver; compared with *Anoectochilus roxburghii* and RU21 used alone, it has a superior hangover-relieving and liver-protecting effect; at the same time, it enriches the deep-processed products of *Anoectochilus roxburghii* on the market and contributes to the healthy and sustainable development of the industry. Attached Figure Description

[0015] Figure 1 This is a typical diagram of the movement trajectory of zebrafish after treatment with the hangover relief and liver protection extract of Embodiment 1 of the present invention (black line represents slow movement, green line represents medium movement, and red line represents fast movement). Figure 2 This is a diagram showing the total movement distance of zebrafish after treatment with the hangover-relieving and liver-protecting syrup from Example 1 of the present invention. Figure 3 This is a photograph of the intensity of fat staining in zebrafish liver after treatment with the hangover relief and liver protection original liquid of Example 1 of the present invention (the yellow dashed box represents the zebrafish liver). Figure 4 This is a graph showing the intensity of fat staining in the liver of zebrafish after treatment with the hangover-relieving and liver-protecting syrup from Example 1 of the present invention. Detailed Implementation

[0016] The technical solution of the present invention will be described in detail below through embodiments, but the scope of protection of the present invention is not limited to the embodiments described.

[0017] Example 1

[0018] This invention provides a *Anoectochilus roxburghii* (Golden Thread Orchid) hangover-relieving and liver-protecting raw material, which is prepared by a method including the following steps: 1) Weigh out 300g of *Anoectochilus roxburghii* juice, 180g of bayberry juice, and 180g of goji berry juice. Add 10 times the total weight of water, mix and blend into a pulp. Pass the pulp through a 200-mesh sieve to remove impurities and obtain the base pulp. The above-mentioned *Anoectochilus roxburghii* juice, bayberry juice, and goji berry juice are obtained by directly blending fresh *Anoectochilus roxburghii*, fresh bayberry, and fresh goji berries. Since the harvesting period of *Anoectochilus roxburghii*, bayberry, and goji berries is short, they can be blended in batches after harvesting and frozen for later use.

[0019] 2) Transfer the above raw slurry base to a vacuum homogenizing emulsifier. At 40°C and 10,000 rpm, slowly add 30g of pseudomicrophyllum oil for homogenization for 15 minutes to form a preliminary emulsion. The above pseudomicrophyllum oil is a commercially available product.

[0020] 3) Under food-grade nitrogen protection, add 5g of cherry blossom polyphenols and 5g of proanthocyanidins in sequence, and homogenize at a pressure of 1300 bar for 3 times, 8 minutes each time, to obtain the original pulp; the above cherry blossom polyphenols and proanthocyanidins are commercially available products.

[0021] 4) Sterilize using ultra-high pressure low temperature sterilization technology. Pass the above raw pulp into HPP sterilization equipment (HPPower-150, Shanghai Zhirui Precision Equipment Co., Ltd.) and sterilize it at 500Mpa for 5 minutes. Then fill it with nitrogen in a sterile environment and store it at 4℃. The raw pulp of *Anoectochilus roxburghii* for hangover relief and liver protection is obtained and is referred to as raw pulp 1.

[0022] Example 2

[0023] The ingredients in Example 1, namely "300g of Anoectochilus roxburghii juice, 180g of bayberry juice, 180g of wolfberry juice", "30g of Micrococcus pluvialis oil", "5g of cherry blossom polyphenols", and "5g of proanthocyanidins", were changed to "250g of Anoectochilus roxburghii juice, 160g of bayberry juice, 160g of wolfberry juice", "20g of Micrococcus pluvialis oil", "4g of cherry blossom polyphenols", and "4g of proanthocyanidins". Otherwise, the same as in Example 1 was used to prepare Anoectochilus roxburghii alcohol-relieving and liver-protecting raw paste, which was designated as Raw Paste 2.

[0024] Example 3 The ingredients in Example 1, namely “300g of Anoectochilus roxburghii juice, 180g of bayberry juice, 180g of wolfberry juice,” “30g of Micrococcus pluvialis oil,” “5g of cherry blossom polyphenols,” and “5g of proanthocyanidins,” were changed to “300g of Anoectochilus roxburghii juice, 180g of bayberry juice, 180g of wolfberry juice,” “15g of Micrococcus pluvialis oil,” “2g of cherry blossom polyphenols,” and “2g of proanthocyanidins.” Otherwise, the preparation was the same as in Example 1, and the Anoectochilus roxburghii hangover-relieving and liver-protecting raw paste was prepared, which was designated as Raw Paste 3.

[0025] Performance testing 1. Hangover relief function

[0026] Experimental groups: normal control group, model control group, RU21 group (concentration of 100 μg / mL) (positive group), and sample group (using the original pulp 1 of Example 1, with 3 concentrations: 1.25 μL / mL, 2.5 μL / mL, and 5 μL / mL).

[0027] Methods: Zebrafish (wild-type AB strain, Baihuan Biotechnology (Guangzhou) Co., Ltd.) 5 days post-fertilization (5 dpf) were randomly selected and exposed to samples in 6-well plates (3 mL / well), with 18 fish per well. The normal control group was cultured normally. The model control group, RU21 group, and sample group were given 2% ethanol to induce a zebrafish hangover model for 1 h. Afterwards, the modeling agent was washed off, and RU21 (concentration: 100 μg / mL, manufacturer: RU-21, LLC; Lot #: 25807) was administered for 1 h. Ten zebrafish from each experimental group were randomly selected and placed in a behavior analyzer (manufacturer: Viewpoint ZebraLab; serial number: ZEB6194) for 1 h of behavioral measurement. The standard was set at 110 mm in length for the 96-well plate. Software analysis was then used to evaluate the hangover relief efficacy of the samples by counting the total movement distance of the zebrafish. Results are shown in Table 1. Figures 1-2 As shown.

[0028] Table 1. Experimental results of the hangover relief efficacy of the samples (n = 10)

[0029] Compared with the model control group, * represents p<0.05, ** represents p<0.01, and *** represents p<0.001. As shown in Table 1, when the concentration of raw material 1 was 2.5 μL / mL, the total movement distance of zebrafish was significantly increased compared with that of the model control group, revealing that the sample had the effect of relieving hangovers. Figure 1 and Figure 2 The same conclusion was reached.

[0030] 2. Liver-protecting effects Experimental groups: normal control group, model control group, RU21 group (concentration of 100 μg / mL) (positive group), and sample group (using the original pulp 1 of Example 1, with 3 concentrations: 1.25 μL / mL, 2.5 μL / mL, and 5 μL / mL).

[0031] Methods: Zebrafish (wild-type AB strain, Baihuan Biotechnology (Guangzhou) Co., Ltd.) 5 days post-fertilization (5 dpf) were randomly selected and exposed to RU21 (concentration: 100 μg / mL) in 6-well plates (3 mL / well), with 18 fish per well. The normal control group was cultured normally, while the model control group, RU21 group, and sample group were given 1% ethanol to induce a zebrafish alcoholic fatty liver model for 30 h. The zebrafish were fixed, stained with Oil Red O, and then destained. Ten zebrafish from each experimental group were randomly selected and photographed under a dissecting microscope. The intensity of hepatoprotective effects was evaluated by statistically analyzing the intensity of hepatoprotective staining in the liver. Results are shown in Table 2. Figures 3-4 As shown.

[0032] Table 2. Experimental results of liver-protective efficacy of the samples (n = 10)

[0033] Compared with the model control group, * represents p<0.05, *** represents p<0.001. As shown in Table 2, when the concentration of raw material 1 was 5 μL / mL, the intensity of fat staining in zebrafish liver was significantly reduced compared with that in the model control group, revealing that the sample had a liver-protective effect. Figure 3 and Figure 4 The same conclusion was reached.

[0034] Unless otherwise specified, all technologies mentioned above refer to existing technologies.

[0035] As described above, although the invention has been shown and described with reference to specific preferred embodiments, it should not be construed as limiting the invention itself. Various changes in form and detail may be made without departing from the spirit and scope of the invention as defined in the appended claims.

Claims

1. A kind of *Anoectochilus roxburghii* (a type of orchid) hangover-relieving and liver-protecting extract, characterized in that, It is made from the following ingredients in parts by weight: 150-350 parts of Anoectochilus roxburghii juice, 100-250 parts of bayberry juice, 100-250 parts of wolfberry juice, 10-30 parts of Micrococcus pseudocarpa oil, 1-5 parts of cherry blossom polyphenols, and 1-5 parts of proanthocyanidins. The original juice of Anoectochilus roxburghii, bayberry, and goji berries is obtained by directly blending fresh Anoectochilus roxburghii, fresh bayberry, and fresh goji berries into a puree. The *Anoectochilus roxburghii* hangover-relieving and liver-protecting extract is prepared by the following method: 1) Weigh out the original juice of Anoectochilus roxburghii, bayberry, and wolfberry according to the weight ratio, add water at 10-15 times the total weight of the ingredients, mix and pulp, sieve, remove the residue, and obtain the original pulp base; 2) Transfer the original pulp base to a vacuum homogenizing emulsifier, and slowly add the pseudomicrosphere oil at 38℃-42℃ to homogenize and form a preliminary emulsion; 3) Under nitrogen protection, the cherry blossom polyphenols and proanthocyanidins were added sequentially and homogenized to obtain the original pulp; 4) The raw pulp is sterilized, nitrogen-filled in a sterile environment, and refrigerated.

2. The *Anoectochilus roxburghii* hangover-relieving and liver-protecting extract according to claim 1, characterized in that, It is made from the following ingredients in parts by weight: 200-350 parts of Anoectochilus roxburghii juice, 150-250 parts of bayberry juice, 150-250 parts of wolfberry juice, 12-25 parts of Micrococcus pseudocarpa oil, 2-5 parts of cherry blossom polyphenols, and 2-5 parts of proanthocyanidins.

3. The *Anoectochilus roxburghii* hangover-relieving and liver-protecting extract according to claim 2, characterized in that, It is made from the following ingredients in parts by weight: 250-300 parts of Anoectochilus roxburghii juice, 160-220 parts of bayberry juice, 160-220 parts of wolfberry juice, 18-25 parts of Micrococcus pseudocarpa oil, 3-5 parts of cherry blossom polyphenols, and 3-5 parts of proanthocyanidins.

4. The preparation method of the *Anoectochilus roxburghii* hangover-relieving and liver-protecting extract according to any one of claims 1-3, characterized in that, Includes the following steps: 1) Weigh out the original juice of Anoectochilus roxburghii, bayberry, and wolfberry according to the weight ratio, add water at 10-15 times the total weight of the ingredients, mix and pulp, sieve, remove the residue, and obtain the original pulp base; 2) Transfer the original pulp base to a vacuum homogenizing emulsifier, and slowly add the pseudomicrosphere oil at 38℃-42℃ to homogenize and form a preliminary emulsion; 3) Under nitrogen protection, the cherry blossom polyphenols and proanthocyanidins were added sequentially and homogenized to obtain the original pulp; 4) The raw pulp is sterilized, nitrogen-filled in a sterile environment, and refrigerated.

5. The preparation method of the *Anoectochilus roxburghii* hangover-relieving and liver-protecting syrup according to claim 4, characterized in that, In step 1), a 200-300 mesh sieve is used for sieving.

6. The preparation method of the *Anoectochilus roxburghii* hangover-relieving and liver-protecting syrup according to claim 4, characterized in that, In step 2), the rotation speed of the vacuum homogenizing emulsifier is 8000-12000 rpm, and the homogenization time is 10-20 minutes.

7. The preparation method of the *Anoectochilus roxburghii* hangover-relieving and liver-protecting syrup according to claim 4, characterized in that, In step 3), the homogenization pressure is 1200-1500 bar, the homogenization is performed 3-4 times, and each time it lasts 8-10 minutes.

8. The preparation method of the *Anoectochilus roxburghii* hangover-relieving and liver-protecting syrup according to claim 4, characterized in that, In step 4), the sterilization pressure is 400-600 MPa, the sterilization time is 3-5 minutes, and the refrigerated storage temperature is 2℃-6℃.

Images