Fe-CPS complex extraction method based on dialysis bag and application of Fe-CPS complex extraction method

By using a 100 Da dialysis bag to selectively retain Fe-CPS complexes, the problems of structural disturbance and high energy consumption in the extraction process of existing technologies are solved, achieving efficient and low-cost separation and enrichment of Fe-CPS complexes, and improving their application effect in photodegradation of pollutants.

CN121868908APending Publication Date: 2026-04-17KUNMING UNIV OF SCI & TECH
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Patent Information

Application Number
CN202610036071.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-01-12
Publication Date
2026-04-17

AI Technical Summary

Technical Problem

Existing technologies pose risks of disturbing the binding state and structural characteristics when extracting and enriching Fe-CPS complexes. Furthermore, the separation process is energy-intensive and costly, and improper selection of dialysis effects and operating conditions may lead to the loss of target components.

Method used

The Fe-CPS complex was selectively retained using a dialysis bag with a molecular weight cutoff of 100 Da. Small molecule diffusion was driven by concentration gradient to remove free iron ions and small molecule interfering substances, avoiding transmembrane pressure-driven and organic solvent extraction, thus maintaining the structural stability of the Fe-CPS complex.

Benefits of technology

It achieves efficient separation and enrichment of Fe-CPS complexes under mild conditions, reduces the risk of structural disturbance, improves the retention rate of target components, and promotes the removal of pollutants during photodegradation.

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Abstract

The invention discloses a dialysis bag-based Fe-CPS complex extraction method and application thereof, and belongs to the technical field of water treatment and environmental protection. The method comprises the following steps: inoculating microorganisms into an inorganic salt culture medium containing water-containing iron ore, and carrying out cell disruption, centrifugation and filtration to obtain a solution containing an Fe-CPS complex; then a dialysis bag with the molecular weight cut-off of 100 Da is adopted for separation and enrichment, free iron ions and small molecular impurities can be effectively removed, and selective cut-off of the complex is achieved. The method is mild in condition and simple and convenient in flow, organic solvent extraction or solid-phase adsorption materials are not adopted in the enrichment and separation process, driving is not depended on transmembrane pressure, and the structural integrity and photochemical activity of the Fe-CPS complex can be remarkably kept. The obtained Fe-CPS complex can be used for photodegradation of steroid estrogen 17 beta-estradiol (E2) in a water body. According to the invention, stable extraction and effective application of the Fe-CPS complex are realized, and technical support is provided for related environmental research and photochemical removal of pollutants.
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