Traditional Chinese medicine composition for treating erysipelas and preparation method thereof

Abstract

The invention belongs to the technical field of traditional Chinese medicines, and provides a traditional Chinese medicine composition for treating erysipelas and a preparation method thereof. The traditional Chinese medicine composition comprises the following components: astragalus membranaceus, artemisia anomala, sappan wood, callicarpa macrophylla, winged euonymus twig, honeysuckle, purslane, lithospermum, sanguisorba officinalis, cacumen biotae and golden cypress. Pharmacodynamic experiments show that the traditional Chinese medicine composition obviously relieves lower limb swelling of erysipelas rats, reduces the content of NO in serum, increases the content of TNF-alpha in the serum, reduces the lesion score of lesion parts, and effectively improves related symptoms of erysipelas. The traditional Chinese medicine composition is wide in raw material source, easy to collect, simple in preparation process, high in safety, free of obvious adverse effects and free of adverse effects on the environment.

Description

Technical Field

[0001] This invention belongs to the field of traditional Chinese medicine technology, and relates to a traditional Chinese medicine composition for treating erysipelas and its preparation method. Background Technology

[0002] Erysipelas is an acute lymphangitis caused by group A beta-hemolytic streptococcus. This pathogen typically invades the skin or mucous membranes when there is inflammation or damage, and can also be caused by bloodstream infection. Erysipelas is a common surgical condition, frequently occurring on the face, legs, feet, and abdomen of infants. It typically presents as well-defined, slightly raised, patchy red rashes that blanch upon light pressure and easily spread. The skin surface feels hot and burning, accompanied by high fever, chills, and headache. The disease can recur at the original site, a condition known as recurrent erysipelas. Prolonged illness can lead to chronic lymphedema of the lower extremities, known as elephantiasis, and even streptococcal toxic shock syndrome.

[0003] Traditional Chinese medicine believes that the pathogenesis of erysipelas is due to inherent heat in the blood, combined with external fire toxins, leading to the accumulation of heat toxins that obstruct the skin and cause erysipelas; or it may be caused by skin or mucous membrane damage, allowing pathogenic factors to invade. The occurrence and development of this disease are related to damp heat and fire toxins, with fire toxins being the primary factor. The *Shengji Zonglu* records: "The main cause of heat toxins is a sudden eruption between the skin, which, unable to be released, accumulates heat and becomes erysipelas." The *Zhubing Yuanhou Lun* states: "Erysipelas is a sudden reddening of the body, resembling cinnabar, hence the name 'red'. It may appear on the feet or abdomen, about the size of a palm, all caused by wind-heat and malignant toxins. In severe cases, it can also develop into carbuncles; if not treated urgently, the pain becomes unbearable, and it eventually rots." The pathogenesis of lower limb erysipelas is mostly due to damp heat descending downwards, combined with external pathogenic factors, causing damp-heat toxins to accumulate in the lower limbs, obstructing the skin and meridians. Because dampness is sticky and combines with heat toxins, lower limb erysipelas is prone to recurrence. In short, erysipelas of the lower extremities is closely related to dampness, heat, and toxicity. Therefore, the treatment focuses on clearing heat and relieving fire, supplemented by promoting blood circulation, eliminating dampness, reducing swelling, and dissipating nodules.

[0004] Currently, modern medicine treats erysipelas with systemic anti-infective therapy using penicillin-type drugs that fight Gram-positive cocci, combined with local application of 50% magnesium sulfate wet compresses. This method has definite clinical efficacy and works relatively quickly. However, long-term use of antibiotics can easily lead to antibiotic resistance. Many patients experience "stagnation" after treatment. Studies have also shown that simple antibiotic treatment has problems such as unstable efficacy, long treatment course, and easy recurrence of the disease.

[0005] Despite its advanced development, Western medicine still has limitations and shortcomings in the treatment of some diseases. Meanwhile, the advantages of traditional Chinese medicine are gradually becoming apparent. The rapid development of traditional Chinese medicine can, to some extent, make up for the limitations and shortcomings of Western medicine alone and promote the development of medicine.

[0006] Therefore, there is an urgent need to develop a safe and effective treatment drug to provide new ideas and methods for the treatment of surgical diseases such as erysipelas of the lower extremities, alleviate patients' suffering, and improve their survival rate. Summary of the Invention

[0007] In view of this, the present invention provides a traditional Chinese medicine composition for treating erysipelas and a method for preparing the same.

[0008] The present invention also provides a method for preparing the above-mentioned traditional Chinese medicine composition for treating erysipelas.

[0009] The present invention also provides the application of the above-mentioned traditional Chinese medicine composition for treating erysipelas in the preparation of a drug for treating erysipelas.

[0010] The technical solution adopted by the present invention to achieve the above objectives is as follows: In a first aspect, the present invention provides a traditional Chinese medicine composition for treating erysipelas, the composition comprising the following components: Astragalus membranaceus, Artemisia capillaris, Caesalpinia sappan, Callicarpa macrophylla, Euonymus alatus, Lonicera japonica, Portulaca oleracea, Lithospermum erythrorhizon, Sanguisorba officinalis, Platycladus orientalis, and Phellodendron chinense.

[0011] As some preferred technical solutions of the present invention, the following components are contained in parts by weight: Astragalus membranaceus 20-25 parts, Artemisia capillaris 16-20 parts, Caesalpinia sappan 18-22 parts, Callicarpa macrophylla 16-20 parts, Euonymus alatus 18-22 parts, Lonicera japonica 18-22 parts, Portulaca oleracea 18-22 parts, Lithospermum erythrorhizon 8-12 parts, Sanguisorba officinalis 8-12 parts, Platycladus orientalis leaves 12-18 parts, and Phellodendron chinense 18-22 parts.

[0012] As a preferred technical solution of the present invention, the following components are contained in parts by weight: 22 parts of Astragalus membranaceus, 18 parts of Artemisia capillaris, 20 parts of Sappanwood, 18 parts of Callicarpa macrophylla, 20 parts of Euonymus alatus, 20 parts of Lonicera japonica, 20 parts of Portulaca oleracea, 10 parts of Lithospermum erythrorhizon, 10 parts of Sanguisorba officinalis, 15 parts of Platycladus orientalis, and 20 parts of Phellodendron chinense.

[0013] As a preferred technical solution of the present invention, the following components are contained in parts by weight: 25 parts of Astragalus membranaceus, 16 parts of Artemisia capillaris, 18 parts of Sappanwood, 20 parts of Callicarpa macrophylla, 22 parts of Euonymus alatus, 22 parts of Lonicera japonica, 18 parts of Portulaca oleracea, 12 parts of Lithospermum erythrorhizon, 8 parts of Sanguisorba officinalis, 12 parts of Platycladus orientalis leaves, and 22 parts of Phellodendron chinense.

[0014] As a preferred technical solution of the present invention, the following components are contained in parts by weight: 22 parts of Astragalus membranaceus, 18 parts of Artemisia capillaris, 22 parts of Caesalpinia sappan, 16 parts of Callicarpa macrophylla, 18 parts of Euonymus alatus, 20 parts of Lonicera japonica, 22 parts of Portulaca oleracea, 8 parts of Lithospermum erythrorhizon, 10 parts of Sanguisorba officinalis, 15 parts of Platycladus orientalis, and 20 parts of Phellodendron chinense.

[0015] In a second aspect, the present invention provides a method for preparing a traditional Chinese medicine composition for treating erysipelas, comprising the following steps: weighing Astragalus membranaceus, Artemisia capillaris, Caesalpinia sappan, Callicarpa macrophylla, Euonymus alatus, Lonicera japonica, Portulaca oleracea, Lithospermum erythrorhizon, Sanguisorba officinalis, Platycladus orientalis, and Phellodendron chinense, mixing them, adding water in a volume 7-10 times the total weight for the first time, decocting for 1-1.5 hours, filtering to obtain a filtrate, adding water in a volume 5-8 times the total weight of the filter residue to the filter residue, decocting for 0.5-1 hours, filtering to obtain a filtrate, and combining it with the filtrate obtained from the first decoction.

[0016] As some preferred technical solutions of the present invention, the preparation method includes the following steps: weighing Astragalus membranaceus, Artemisia capillaris, Sappanwood, Callicarpa macrophylla, Euonymus alatus, Lonicera japonica, Portulaca oleracea, Lithospermum erythrorhizon, Sanguisorba officinalis, Platycladus orientalis leaves, and Phellodendron chinense, mixing them, adding water in a total weight of 8 times for the first time, decocting for 1 hour, filtering to obtain filtrate, adding water in a total weight of 6 times for the filter residue, decocting for 1 hour, filtering to obtain filtrate, and combining it with the filtrate obtained from the first decoction.

[0017] A third aspect of the invention provides the use of the traditional Chinese medicine composition in the preparation of a medicine for the prevention or treatment of erysipelas.

[0018] The beneficial effects of the traditional Chinese medicine composition for treating erysipelas of the present invention are as follows: External treatments in Traditional Chinese Medicine allow the active ingredients of medications to directly contact the skin, producing therapeutic effects or reaching the lesion directly during transdermal absorption. The effects are obvious and rapid. At the same time, the treatment can accelerate local blood circulation and nutrient supply, quickly clear metabolic waste, inflammatory exudates and pain-causing substances from the painful area, reduce symptoms, relieve patient pain, and reduce recurrence.

[0019] In the herbal composition of this invention, Astragalus membranaceus, Artemisia capillaris, Sappanwood, and Euonymus alatus are the principal herbs. Astragalus membranaceus tonifies Qi, promotes blood circulation, and eliminates toxins, serving as the core of "strengthening the body's resistance." Artemisia capillaris, Sappanwood, and Euonymus alatus work synergistically to address the symptoms of "blood stasis and toxins," with the four herbs working together to achieve the effects of "strengthening the body's resistance and eliminating toxins + breaking up blood stasis and dispersing nodules + promoting blood circulation and relieving pain + breaking up blood stasis and detoxifying." Honeysuckle and Phellodendron amurense clear damp-heat in the Qi level and lower Jiao, while Lithospermum erythrorhizon cools blood stasis and heat. These three herbs work together to assist the principal herbs, targeting the "residual heat toxins" that may remain in chronic erysipelas. At the same time, their cooling properties can also counteract the warming and drying properties of Artemisia capillaris and Sappanwood in the principal herbs. Sanguisorba officinalis assists Astragalus membranaceus in promoting tissue regeneration and healing sores; Platycladus orientalis leaves, Portulaca oleracea, and Callicarpa macrocarpa all have hemostatic effects, working together to counteract the strong blood-breaking properties of the principal herb, preventing it from consuming or disturbing blood, embodying the principle of "no harm if there is a cause, and no harm if there is not." Callicarpa macrocarpa, in particular, combines the effects of dispersing blood stasis and stopping bleeding, forming a delicate balance with the principal herb of "dispersing within breaking up blood stasis, and stopping bleeding within dispersing blood stasis." This formula uses appropriate herbs, addresses the pathogenesis, and works together to clear heat and detoxify, cool the blood and dry dampness.

[0020] Pharmacodynamic experiments showed that the herbal composition of this invention significantly relieved lower limb swelling in erysipelas rats, reduced serum NO content, reduced serum TNF-α content, lowered lesion scores at the lesion sites, and effectively improved related symptoms of erysipelas.

[0021] The raw materials for the traditional Chinese medicine composition of this invention are widely available, easy to collect, have a simple preparation process, are highly safe, have no obvious adverse effects, and have no adverse impact on the environment. Attached Figure Description

[0022] Figure 1 Comparison of the diameter of swollen hind limbs in each group of rats with that of the model control group. P <0.05, P <0.01; compared with Experimental Group 1, # P <0.05, ## P <0.01.

[0023] Figure 2 Comparison of serum NO levels in rats of different groups with the blank control. & P <0.05, && P <0.01; compared with the model control group, P <0.05, P <0.01; compared with Experimental Group 1, # P <0.05, ## P <0.01.

[0024] Figure 3 Comparison of TNF-α levels in the serum of rats in each group with the blank control. & P <0.05, && P <0.01; compared with the model control group, P <0.05, P <0.01; compared with Experimental Group 1, # P <0.05, ## P <0.01.

[0025] Figure 4 Comparison of lesion scores among different groups of rats with the blank control group. & P<0.05, && P <0.01; compared with the model control group, P <0.05, P <0.01; compared with Experimental Group 1, # P <0.05, ## P <0.01. Detailed Implementation

[0026] Exemplary embodiments will now be described more fully with reference to the accompanying drawings. However, these exemplary embodiments can be implemented in many forms and should not be construed as limited to the examples set forth herein; rather, they are provided so that the description of this disclosure will be more complete and fully convey the concept of the exemplary embodiments to those skilled in the art.

[0027] Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. While only preferred methods and materials have been described herein, any methods and materials similar or equivalent to those described herein may be used in the implementation or testing of this invention. All references to this specification are incorporated by way of citation to disclose and describe methods and / or materials associated with those references. In the event of any conflict with any incorporated reference, the content of this specification shall prevail.

[0028] Unless otherwise specified, the instruments, reagents, and materials used in the following embodiments are all conventional instruments, reagents, and materials already available in the prior art and can be obtained through legitimate commercial channels. Unless otherwise specified, the experimental methods and detection methods used in the following embodiments are all conventional experimental methods and detection methods already available in the prior art.

[0029] Example 1 This embodiment provides a traditional Chinese medicine composition for treating erysipelas, which is composed of the following raw materials: 22 parts Astragalus membranaceus, 18 parts Artemisia capillaris, 20 parts Caesalpinia sappan, 18 parts Callicarpa macrophylla, 20 parts Euonymus alatus, 20 parts Lonicera japonica, 20 parts Portulaca oleracea, 10 parts Lithospermum erythrorhizon, 10 parts Sanguisorba officinalis, 15 parts Platycladus orientalis, and 20 parts Phellodendron chinense.

[0030] The preparation method of this traditional Chinese medicine composition is as follows: Weigh out Astragalus membranaceus, Artemisia capillaris, Sappanwood, Callicarpa macrophylla, Euonymus alatus, Lonicera japonica, Portulaca oleracea, Lithospermum erythrorhizon, Sanguisorba officinalis, Platycladus orientalis leaves, and Phellodendron chinense. Mix them together. Add water equal to 8 times the total weight of the ingredients for the first time, decoct for 1 hour, filter, and obtain the filtrate. Add water equal to 6 times the total weight of the residue to the residue, decoct for 1 hour, filter, and obtain the filtrate. Combine the filtrate obtained from the first decoction with the filtrate obtained from the first decoction.

[0031] Example 2 This embodiment provides a traditional Chinese medicine composition for treating erysipelas, which is composed of the following raw materials: 25 parts of Astragalus membranaceus, 16 parts of Artemisia capillaris, 18 parts of Caesalpinia sappan, 20 parts of Callicarpa macrophylla, 22 parts of Euonymus alatus, 22 parts of Lonicera japonica, 18 parts of Portulaca oleracea, 12 parts of Lithospermum erythrorhizon, 8 parts of Sanguisorba officinalis, 12 parts of Platycladus orientalis, and 22 parts of Phellodendron chinense.

[0032] The preparation method of this traditional Chinese medicine composition is as follows: Weigh out Astragalus membranaceus, Artemisia capillaris, Sappanwood, Callicarpa macrophylla, Euonymus alatus, Lonicera japonica, Portulaca oleracea, Lithospermum erythrorhizon, Sanguisorba officinalis, Platycladus orientalis leaves, and Phellodendron chinense. Mix them together. Add water with a total weight of 10 times the weight of the ingredients for the first time, decoct for 1 hour, filter, and obtain the filtrate. Add water with a total weight of 5 times the weight of the residue to the residue, decoct for 0.5 hours, filter, and obtain the filtrate. Combine the filtrate obtained from the first decoction with the filtrate obtained from the first decoction.

[0033] Example 3 This embodiment provides a traditional Chinese medicine composition for treating erysipelas, which is composed of the following raw materials: 22 parts Astragalus membranaceus, 18 parts Artemisia capillaris, 22 parts Caesalpinia sappan, 16 parts Callicarpa macrophylla, 18 parts Euonymus alatus, 20 parts Lonicera japonica, 22 parts Portulaca oleracea, 8 parts Lithospermum erythrorhizon, 10 parts Sanguisorba officinalis, 15 parts Platycladus orientalis, and 20 parts Phellodendron chinense.

[0034] The preparation method of this traditional Chinese medicine composition is as follows: Weigh out Astragalus membranaceus, Artemisia capillaris, Sappanwood, Callicarpa macrophylla, Euonymus alatus, Lonicera japonica, Portulaca oleracea, Lithospermum erythrorhizon, Sanguisorba officinalis, Platycladus orientalis leaves, and Phellodendron chinense. Mix them together. Add water equal to 8 times the total weight of the ingredients for the first time, decoct for 1.5 hours, filter, and obtain the filtrate. Add water equal to 6 times the total weight of the residue to the residue, decoct for 1 hour, filter, and obtain the filtrate. Combine the filtrate obtained from the first decoction with the filtrate obtained from the first decoction.

[0035] Example 4 This embodiment provides a traditional Chinese medicine composition for treating erysipelas, which is composed of the following raw materials: 20 parts of Astragalus membranaceus, 20 parts of Artemisia capillaris, 22 parts of Sappanwood, 18 parts of Callicarpa macrophylla, 20 parts of Euonymus alatus, 18 parts of Lonicera japonica, 20 parts of Portulaca oleracea, 10 parts of Lithospermum erythrorhizon, 12 parts of Sanguisorba officinalis, 18 parts of Platycladus orientalis, and 18 parts of Phellodendron chinense.

[0036] The preparation method of this traditional Chinese medicine composition is as follows: Weigh out Astragalus membranaceus, Artemisia capillaris, Sappanwood, Callicarpa macrophylla, Euonymus alatus, Lonicera japonica, Portulaca oleracea, Lithospermum erythrorhizon, Sanguisorba officinalis, Platycladus orientalis leaves, and Phellodendron chinense, mix them, add water with 10 times the total weight for the first time, decoct for 1.5 hours, filter, and obtain filtrate. Add water with 8 times the total weight of the filter residue to the filter residue, decoct for 0.5 hours, filter, and obtain filtrate. Combine the filtrate obtained from the first decoction with the filtrate obtained from the first decoction.

[0037] Example 5 This embodiment provides a traditional Chinese medicine composition for treating erysipelas, which is composed of the following raw materials: 25 parts of Astragalus membranaceus, 18 parts of Artemisia capillaris, 20 parts of Sappanwood, 18 parts of Callicarpa macrophylla, 18 parts of Euonymus alatus, 20 parts of Lonicera japonica, 22 parts of Portulaca oleracea, 8 parts of Lithospermum erythrorhizon, 12 parts of Sanguisorba officinalis, 15 parts of Platycladus orientalis, and 18 parts of Phellodendron chinense.

[0038] The preparation method of this traditional Chinese medicine composition is as follows: Weigh out Astragalus membranaceus, Artemisia capillaris, Sappanwood, Callicarpa macrophylla, Euonymus alatus, Lonicera japonica, Portulaca oleracea, Lithospermum erythrorhizon, Sanguisorba officinalis, Platycladus orientalis leaves, and Phellodendron chinense, mix them, add water with a total weight of 7 times the amount of water for the first time, decoct for 1 hour, filter, and obtain filtrate. Add water with a total weight of 6 times the amount of water for the residue, decoct for 1 hour, filter, and obtain filtrate. Combine the filtrate obtained from the first decoction with the filtrate obtained from the first decoction.

[0039] Example 6 This embodiment provides a traditional Chinese medicine composition for treating erysipelas, which is composed of the following raw materials: 22 parts of Astragalus membranaceus, 16 parts of Artemisia capillaris, 18 parts of Caesalpinia sappan, 20 parts of Callicarpa macrophylla, 22 parts of Euonymus alatus, 18 parts of Lonicera japonica, 20 parts of Portulaca oleracea, 12 parts of Lithospermum erythrorhizon, 10 parts of Sanguisorba officinalis, 18 parts of Platycladus orientalis, and 22 parts of Phellodendron chinense.

[0040] The preparation method of this traditional Chinese medicine composition is as follows: Weigh out Astragalus membranaceus, Artemisia capillaris, Sappanwood, Callicarpa macrophylla, Euonymus alatus, Lonicera japonica, Portulaca oleracea, Lithospermum erythrorhizon, Sanguisorba officinalis, Platycladus orientalis leaves, and Phellodendron chinense. Mix them together. Add water equal to 8 times the total weight of the ingredients for the first time, decoct for 1.5 hours, filter, and obtain the filtrate. Add water equal to 8 times the total weight of the residue to the residue, decoct for 0.5 hours, filter, and obtain the filtrate. Combine the filtrate obtained from the first decoction with the filtrate obtained from the first decoction.

[0041] Example 7 This embodiment provides a traditional Chinese medicine composition for treating erysipelas, which is composed of the following raw materials: 25 parts of Astragalus membranaceus, 16 parts of Artemisia capillaris, 18 parts of Caesalpinia sappan, 20 parts of Callicarpa macrophylla, 22 parts of Euonymus alatus, 20 parts of Lonicera japonica, 18 parts of Portulaca oleracea, 10 parts of Lithospermum erythrorhizon, 8 parts of Sanguisorba officinalis, 15 parts of Platycladus orientalis, and 20 parts of Phellodendron chinense.

[0042] The preparation method of this traditional Chinese medicine composition is as follows: Weigh out Astragalus membranaceus, Artemisia capillaris, Sappanwood, Callicarpa macrophylla, Euonymus alatus, Lonicera japonica, Portulaca oleracea, Lithospermum erythrorhizon, Sanguisorba officinalis, Platycladus orientalis leaves, and Phellodendron chinense. Mix them together. Add water equal to 8 times the total weight of the ingredients for the first time, decoct for 1 hour, filter, and obtain the filtrate. Add water equal to 6 times the total weight of the residue to the residue, decoct for 1 hour, filter, and obtain the filtrate. Combine the filtrate obtained from the first decoction with the filtrate obtained from the first decoction.

[0043] Comparative Example 1 18 portions of Artemisia annua, 20 portions of Sappanwood, 20 portions of Euonymus alatus, 20 portions of Lonicera japonica, 20 portions of Portulaca oleracea, 10 portions of Lithospermum erythrorhizon, 10 portions of Sanguisorba officinalis, 15 portions of Platycladus orientalis leaves, and 20 portions of Phellodendron chinense.

[0044] This comparative example is different from Example 1 only in that the component Astragalus membranaceus is not used in the specific formulation.

[0045] Comparative Example 2 22 parts of Astragalus membranaceus, 20 parts of Sappanwood, 20 parts of Winged Euonymus, 20 parts of Honeysuckle, 20 parts of Purslane, 10 parts of Sanguisorba officinalis, 15 parts of Biota orientalis, 20 parts of Phellodendron amurense.

[0046] This comparative example is different from Example 1 only in that the component Artemisia anomala is not used in the specific formulation.

[0047] Comparative Example 3 22 parts of Astragalus membranaceus, 18 parts of Artemisia anomala, 20 parts of Sappanwood, 20 parts of Spatholobus suberectus, 20 parts of Honeysuckle, 20 parts of Purslane, 10 parts of Lithospermum erythrorhizon, 10 parts of Sanguisorba officinalis, 15 parts of Biota orientalis, 20 parts of Phellodendron amurense.

[0048] This comparative example is different from Example 1 only in that Spatholobus suberectus is used to replace Winged Euonymus in the specific formulation.

[0049] Comparative Example 4 22 parts of Astragalus membranaceus, 18 parts of Artemisia anomala, 20 parts of Sappanwood, 18 parts of Callicarpa macrophylla Vahl, 20 parts of Winged Euonymus, 20 parts of Honeysuckle, 20 parts of Purslane, 10 parts of Lithospermum erythrorhizon, 10 parts of Sanguisorba officinalis, 15 parts of Biota orientalis, 20 parts of Sophora flavescens.

[0050] This comparative example is different from Example 1 only in that Sophora flavescens is used to replace Phellodendron amurense in the specific formulation.

[0051] Comparative Example 5 The formulation disclosed in the literature (Tian Yan, Liu Jian, Wang Qinghai, etc. Observation on the curative effect of the compatibility of traditional Chinese medicines for clearing heat, resolving turbidity and promoting diuresis in the treatment of eczema [J]. Shaanxi Journal of Traditional Chinese Medicine, 2012, 33(04): 455-456.): 20 g each of Scutellaria baicalensis, Purslane, Forsythia suspensa, Coix lacryma-jobi, Lithospermum erythrorhizon, 10 g each of Sanguisorba officinalis, Schizonepeta tenuifolia, Saposhnikovia divaricata, Phellodendron amurense, Atractylodes lancea, Sophora flavescens, Rehmannia glutinosa.

[0052] Prepared by referring to the method of Example 1.

[0053] Experimental study of the traditional Chinese medicine composition of the present invention on rats with erysipelas 1. Experimental method 60 healthy male SD rats, weighing 180-220 g, were fed with feed and water freely. The rats were adaptively fed for 7 d and randomly divided into a blank control group and a model group.

[0054] The rats in the model group were injected intradermally with group A beta-hemolytic streptococcus in the right lower limb for 5 consecutive days, with a dosage of 0.08 mL of the stock solution each time, to establish an erysipelas model.

[0055] Two days after modeling, grouped experimental treatments were initiated. The rats after modeling were randomly divided into the following groups according to their body weight: model control group, experimental group 1, experimental group 2, experimental group 3, control group 1, control group 2, control group 3, control group 4, and control group 5, with 6 rats in each group.

[0056] In Experiment 1, the drug solution from Example 1 was administered at 9.5 g / kg (based on crude drug weight); in Experiment 2, the drug solution from Example 2 was administered at 9.5 g / kg (based on crude drug weight); in Experiment 3, the drug solution from Example 3 was administered at 9.5 g / kg (based on crude drug weight); in Control 1, the drug solution from Comparative Example 1 was administered at 9.5 g / kg (based on crude drug weight); in Control 2, the drug solution from Comparative Example 2 was administered at 9.5 g / kg (based on crude drug weight); in Control 3, the drug solution from Comparative Example 3 was administered at 9.5 g / kg (based on crude drug weight); in Control 4, the drug solution from Comparative Example 4 was administered at 9.5 g / kg (based on crude drug weight); and in Control 5, the drug solution from Comparative Example 5 was administered at 9.5 g / kg (based on crude drug weight).

[0057] Each treatment group was fumigated at the right side infection site of rats for 20 minutes at a temperature of 60℃.

[0058] Rats in the blank control group and the model control group were fumigated with an equal volume of distilled water. Each group of rats was administered the drug once daily.

[0059] 2. Indicator Testing 1. Observe the edema at the injection site of the rats every day and measure its average diameter (the sum of the diameters of the horizontal axis and the vertical axis / 2).

[0060] 2. After the last administration, fast for 6 hours, collect blood from the orbital cavity, centrifuge at 3000 r / min for 10 min, and collect serum for determination of NO and TNF-α levels.

[0061] 3. Rats were euthanized by cervical dislocation, and tissue from the inflamed area was collected, fixed with 10% formaldehyde solution, and subjected to histopathological examination. Pathological changes were observed under an optical microscope.

[0062] The reference standard is: a. Lesion extent: One low-power field is used as the scoring unit, i.e., 1 point, two low-power fields are worth 2 points, and so on.

[0063] b. Inflammation and necrosis: The severity of the lesions is scored from mild to severe as follows: 0.5 points (mild), 1 point (mild), 2 points (moderate), 3 points (severe), 4 points (extremely severe), and 0 points (no obvious lesions).

[0064] c. Granulation tissue: Granulation tissue represents the repair of damage. The higher the degree of repair, the lower the score. It is expressed by a 3-level system. No granulation tissue in the necrotic area is 3 points, and the necrotic area is completely replaced by granulation tissue is 0 points.

[0065] d. Fibrous tissue hyperplasia: Fibrous tissue represents the repair of damage. The higher the degree of repair, the lower the score. It is divided into 4 grades based on the presence of fibrous tissue around the lesion. 4 points for no fibrous tissue surrounding the lesion, and 0 points for being completely surrounded by fibrous tissue.

[0066] The scores are summed up, and the average score for each animal in each group is calculated. The results are then compared between groups.

[0067] 3. Statistical Methods Data processing was performed using GraphPad Prism 8.0 software, with the measurement data presented in... ±s indicates the mean. One-way ANOVA was used for comparisons between groups, and P < 0.05 was considered statistically significant.

[0068] 4. Experimental Results Comparison of hind limb swelling results among different groups of rats Table 1 Comparison of hind limb swelling results in different groups of rats Note: Compared with the model control group, P <0.05, P <0.01; compared with Experimental Group 1, # P <0.05, ## P <0.01.

[0069] Compared with the blank control group, the diameter of the swollen area in the rats of the model control group was larger.

[0070] Compared with the model control group, the diameter of the swollen area in rats in experimental groups 1, 2, 3, and the control group was smaller. Compared with control groups 1-5, the diameter of the swollen area in experimental groups 1, 2, and 3 was even smaller. Results are shown in Table 1. Figure 1 .

[0071] Comparison of serum NO levels in rats of different groups Compared with the blank control group, the serum NO content of rats in the model control group was significantly increased.

[0072] Compared with the model control group, the serum NO levels in rats in experimental groups 1, 2, 3, and the control group were significantly reduced. Compared with control groups 1-5, the serum NO levels in rats in experimental groups 1, 2, and 3 were significantly reduced. Results are shown below. Figure 2 .

[0073] Comparison of serum TNF-α levels in different groups of rats Compared with the blank control group, the serum TNF-α content of rats in the model control group was significantly increased.

[0074] Compared with the model control group, the serum TNF-α levels of rats in experimental groups 1, 2, 3, and the control group were significantly reduced. Compared with control groups 1-5, the serum TNF-α levels of rats in experimental groups 1, 2, and 3 were significantly reduced. Results are shown below. Figure 3 .

[0075] Comparison of lesion scores among different groups of rats Compared with the blank control group, the lesion scores of rats in the model control group were significantly increased.

[0076] Compared with the model control group, the lesion scores of rats in experimental groups 1, 2, 3, and the control group were significantly reduced. Compared with control groups 1-5, the lesion scores of rats in experimental groups 1, 2, and 3 were significantly reduced. Results are shown below. Figure 4 .

[0077] In summary, the traditional Chinese medicine composition of this invention significantly reduces lower limb swelling in erysipelas rats, decreases serum NO and TNF-α levels, lowers lesion scores, and effectively improves related symptoms of erysipelas.

[0078] The efficacy of the traditional Chinese medicine compositions prepared in Examples 1-3 and Comparative Examples 1-5 was determined. It was found that Examples 1-3 showed a more significant effect in treating erysipelas compared with Comparative Examples 1-5.

[0079] Further analysis revealed that Astragalus membranaceus, Artemisia capillaris, Euonymus alatus, and Phellodendron chinense play crucial roles in this traditional Chinese medicine composition. When these four ingredients are absent, the synergistic effect of the entire composition is weakened, resulting in a lower efficacy in treating erysipelas and a lesser degree of symptom improvement compared to Examples 1-3, which include Astragalus membranaceus, Artemisia capillaris, Euonymus alatus, and Phellodendron chinense. This indicates that Astragalus membranaceus, Artemisia capillaris, Euonymus alatus, and Phellodendron chinense are indispensable components of this traditional Chinese medicine composition and are key to enhancing its overall efficacy.

[0080] Comparative Example 5 consisted of 20g each of Scutellaria baicalensis, Portulaca oleracea, Forsythia suspensa, Coix lacryma-jobi, and Lithospermum erythrorhizon, and 10g each of Sanguisorba officinalis, Schizonepeta tenuifolia, Saposhnikovia divaricata, Phellodendron chinense, Atractylodes lancea, Sophora flavescens, and Rehmannia glutinosa. The efficacy of Comparative Example 5 in improving erysipelas was not as good as that of the herbal composition of this invention. Compared with Comparative Example 5, the herbal composition of this invention has significant technical advantages.

[0081] The foregoing has only described certain exemplary embodiments of the present invention by way of illustration. Undoubtedly, those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the foregoing drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. A traditional Chinese medicine composition for treating erysipelas, characterized in that, The traditional Chinese medicine composition contains the following components by weight: Astragalus membranaceus 20-25 parts, Artemisia capillaris 16-20 parts, Caesalpinia sappan 18-22 parts, Callicarpa macrophylla 16-20 parts, Euonymus alatus 18-22 parts, Lonicera japonica 18-22 parts, Portulaca oleracea 18-22 parts, Lithospermum erythrorhizon 8-12 parts, Sanguisorba officinalis 8-12 parts, Platycladus orientalis 12-18 parts, and Phellodendron chinense 18-22 parts.

2. The traditional Chinese medicine composition according to claim 1, characterized in that, The traditional Chinese medicine composition contains the following components by weight: Astragalus membranaceus 22 parts, Artemisia capillaris 18 parts, Sappanwood 20 parts, Callicarpa macrophylla 18 parts, Euonymus alatus 20 parts, Lonicera japonica 20 parts, Portulaca oleracea 20 parts, Lithospermum erythrorhizon 10 parts, Sanguisorba officinalis 10 parts, Platycladus orientalis 15 parts, and Phellodendron chinense 20 parts.

3. The traditional Chinese medicine composition according to claim 1, characterized in that, The traditional Chinese medicine composition contains the following components by weight: Astragalus membranaceus 25 parts, Artemisia capillaris 16 parts, Sappanwood 18 parts, Callicarpa macrophylla 20 parts, Euonymus alatus 22 parts, Lonicera japonica 22 parts, Portulaca oleracea 18 parts, Lithospermum erythrorhizon 12 parts, Sanguisorba officinalis 8 parts, Platycladus orientalis 12 parts, and Phellodendron chinense 22 parts.

4. The traditional Chinese medicine composition according to claim 1, characterized in that, The traditional Chinese medicine composition contains the following components by weight: Astragalus membranaceus 22 parts, Artemisia capillaris 18 parts, Sappanwood 22 parts, Callicarpa macrophylla 16 parts, Euonymus alatus 18 parts, Lonicera japonica 20 parts, Portulaca oleracea 22 parts, Lithospermum erythrorhizon 8 parts, Sanguisorba officinalis 10 parts, Platycladus orientalis 15 parts, and Phellodendron chinense 20 parts.

5. A method for preparing a traditional Chinese medicine composition according to any one of claims 1-4, characterized in that, The preparation method includes the following steps: weigh out Astragalus membranaceus, Artemisia capillaris, Sappanwood, Callicarpa macrophylla, Euonymus alatus, Lonicera japonica, Portulaca oleracea, Lithospermum erythrorhizon, Sanguisorba officinalis, Platycladus orientalis leaves, and Phellodendron chinense, mix them together, add 7-10 times the total weight of water for the first time, decoct for 1-1.5 hours, filter to obtain filtrate, add 5-8 times the total weight of water to the filter residue, decoct for 0.5-1 hours, filter to obtain filtrate, and combine it with the filtrate obtained from the first decoction.

6. The method for preparing the traditional Chinese medicine composition according to claim 5, characterized in that, The preparation method includes the following steps: weigh out Astragalus membranaceus, Artemisia capillaris, Sappanwood, Callicarpa macrophylla, Euonymus alatus, Lonicera japonica, Portulaca oleracea, Lithospermum erythrorhizon, Sanguisorba officinalis, Platycladus orientalis leaves, and Phellodendron chinense, mix them, add water equal to 8 times the total weight for the first time, decoct for 1 hour, filter to obtain filtrate, add water equal to 6 times the total weight of the filter residue to the filter residue, decoct for 1 hour, filter to obtain filtrate, and combine it with the filtrate obtained from the first decoction.

7. The method for preparing the traditional Chinese medicine composition according to claim 5, characterized in that, The preparation method includes the following steps: weigh out Astragalus membranaceus, Artemisia capillaris, Sappanwood, Callicarpa macrophylla, Euonymus alatus, Lonicera japonica, Portulaca oleracea, Lithospermum erythrorhizon, Sanguisorba officinalis, Platycladus orientalis leaves, and Phellodendron chinense, mix them, add water with 10 times the total weight for the first time, decoct for 1 hour, filter to obtain filtrate, add water with 5 times the total weight of the filter residue to the filter residue, decoct for 0.5 hours, filter to obtain filtrate, and combine it with the filtrate obtained from the first decoction.

8. The method for preparing the traditional Chinese medicine composition according to claim 5, characterized in that, The preparation method includes the following steps: weigh out Astragalus membranaceus, Artemisia capillaris, Sappanwood, Callicarpa macrophylla, Euonymus alatus, Lonicera japonica, Portulaca oleracea, Lithospermum erythrorhizon, Sanguisorba officinalis, Platycladus orientalis leaves, and Phellodendron chinense, mix them, add water in a volume 8 times the total weight for the first time, decoct for 1.5 hours, filter to obtain filtrate, add water in a volume 6 times the total weight of the filter residue to the filter residue, decoct for 1 hour, filter to obtain filtrate, and combine it with the filtrate obtained from the first decoction.

9. Use of the traditional Chinese medicine composition according to any one of claims 1-4 in the preparation of a medicine for the prevention or treatment of erysipelas.

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