Quality control primer group and quality control method for human-derived sample
By employing quality control measures using human DNA, RNA, and sex-specific quality control primer sets, the problems of sample confusion and nucleic acid extraction failure in nanopore sequencing technology were resolved. This enabled end-to-end quality control, improved the reliability and reproducibility of sequencing data, and made the technology suitable for the detection of pathogenic microorganisms in human samples.
Patent Information
- Application Number
- CN202610089327.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-01-22
- Publication Date
- 2026-05-15
AI Technical Summary
Existing nanopore sequencing technology suffers from problems such as reagent misaddition, sample contamination, nucleic acid extraction failure, and nucleic acid degradation in pathogen detection, leading to erroneous test results and a lack of comprehensive quality control measures.
A quality control primer set, including human DNA quality control primers, human RNA quality control primers, and sex quality control primers, is used to monitor the sequencing process from multiple dimensions. The quality control primer set kit and quality control methods are provided, including multiplex PCR premix and negative control products, for quality control of human samples.
It enables end-to-end quality control of the sequencing process, reduces human error, ensures experimental accuracy and stability, improves data reliability and reproducibility, meets compliance requirements in high-standard fields, quickly locates the root cause of problems, and saves investigation time and costs.