All-component freeze-dried and direct-amplified improved PCR amplification enzyme, amplification enzyme antibody, buffer solution composition and application of all-component freeze-dried and direct-amplified improved PCR amplification enzyme

By genetically modifying and optimizing PCR reagents, an improved PCR amplification and detection reagent with all components lyophilized has been developed. This solves the problems of low-temperature dependence and complex operation of traditional reagents, enabling room-temperature storage and transportation as well as direct amplification. It is suitable for molecular diagnosis of a variety of pathogens, and provides an efficient and convenient solution, especially in grassroots and field testing.

CN122303186APending Publication Date: 2026-06-30GUANGZHOU VIPOTION BIOTECH
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Patent Information

Application Number
CN202610285350.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-03-10
Publication Date
2026-06-30

AI Technical Summary

Technical Problem

Traditional liquid PCR reagents are limited by their low-temperature dependence, the need for cumbersome sample pretreatment, insufficient anti-interference ability, and poor group compatibility.

Method used

By genetically modifying and optimizing key functional components of PCR, an improved PCR amplification detection reagent that is fully lyophilized and directly amplifiable is developed, including Taq enzyme and reverse transcriptase. An enzyme composition including Taq enzyme and reverse transcriptase is provided. The lyophilization tolerance, anti-interference and thermal stability of the enzymes are optimized through gene mutation. Combined with special buffer and protective agent, the reagent can be stored and transported at room temperature and directly amplified.

Benefits of technology

It enables room temperature storage and transportation of PCR reagents, eliminates the sample pretreatment step, improves the speed and sensitivity of detection, and is suitable for molecular diagnosis of a variety of pathogens, especially providing an efficient and convenient solution for grassroots and field testing.

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Abstract

The invention belongs to the technical field of molecular biology, and discloses an all-component freeze-dried and direct-amplified improved PCR amplification enzyme, an amplification enzyme antibody, a buffer solution composition and application of the all-component freeze-dried and direct-amplified improved PCR amplification enzyme. The invention provides an enzyme combination. The enzyme combination comprises Taq enzyme and reverse transcriptase. The Taq enzyme is obtained by carrying out site-specific mutagenesis on the basis of a wild type Taq enzyme, and has the characteristics of excellent freeze-drying tolerance, interference resistance, thermal stability, amplification specificity and the like; the reverse transcriptase is subjected to site-specific mutagenesis on the basis of wild reverse transcriptase, and the reverse transcriptase has better freeze-drying redissolution activity, thermal stability, reverse transcription efficiency and specificity. The enzyme combination is used for preparing an all-component freeze-dried and direct PCR amplification reagent, and the problems that a traditional reagent depends on low-temperature transportation and storage, needs to be extracted and amplified in sequence, and is tedious in operation and weak in anti-interference capability can be synchronously solved.
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