A method for preparing an alpha-cyclodextrin-based magnetic nanoparticle-antibody conjugate, and products and uses thereof

By utilizing a method for preparing α-cyclodextrin-based magnetic nanoparticle-antibody conjugates, the problem of low antibody-magnetic nanoparticle linkage efficiency was solved by leveraging host-guest interactions and covalent bonds. This method achieves stable linkage and high-purity cell sorting, providing a universal conjugation platform.

CN122352142APending Publication Date: 2026-07-10SOUTHEAST UNIV +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SOUTHEAST UNIV
Filing Date
2026-04-10
Publication Date
2026-07-10

AI Technical Summary

Technical Problem

Existing methods for linking antibodies to magnetic nanoparticles suffer from problems such as low coupling efficiency, poor batch-to-batch reproducibility, and non-specific adsorption. Furthermore, traditional linking methods are complex and affect the purity and efficiency of cell sorting.

Method used

A method for preparing antibody-antibody conjugates based on α-cyclodextrin was adopted. Through host-guest interactions and covalent bonding, stable connection between antibodies and magnetic nanoparticles was achieved. The unique structural design of cyclodextrin simplifies the conjugation steps and improves the reproducibility and versatility of the system.

Benefits of technology

Stable binding of antibodies to magnetic nanoparticles was achieved, improving conjugation efficiency and purity, simplifying the preparation process, and providing a universal conjugation platform suitable for replacing different antibodies and nanoparticles, applicable to fields such as cell sorting.

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Abstract

This invention discloses a method for preparing α-cyclodextrin-based magnetic nanoparticle-antibody conjugates, along with their products and applications. The invention leverages the hydrophobic cavity of α-cyclodextrin to bind with the methoxy PEG on the surface of magnetic nanoparticles via host-guest interactions, achieving a non-covalent bond between α-cyclodextrin and the magnetic nanoparticles. Furthermore, the toluenesulfonyl group modified on the α-cyclodextrin surface undergoes a nucleophilic substitution reaction with the amino group of the antibody, resulting in a stable binding between the antibody and cyclodextrin. This structural design enables a low-cost method for preparing cyclodextrin-based nanoparticle-antibody conjugates. This method offers advantages such as high antibody conjugation rate, simple preparation process, and good biocompatibility, and can be used in fields such as immunomagnetic sorting and targeted drug delivery. It also provides a novel approach and method for the binding of nanoparticles and antibodies.
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