Liquid composition for determining ATP, and amp and / or ADP in a sample

By optimizing the enzyme concentration and luminescence amount in the liquid composition, the problems of complicated preparation and insufficient stability of powdered cyclic luminescent reagents are solved, providing a liquid cyclic luminescent reagent with excellent stability, suitable for the determination of ATP, AMP and/or ADP.

CN122357686APending Publication Date: 2026-07-10KIKKOMAN CORP
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Patent Information

Application Number
CN202610456642.5
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Priority Date
2020-02-14
Filing Date
2021-02-15
Publication Date
2026-07-10

AI Technical Summary

Technical Problem

Existing powdered cyclic luminescent reagents are complicated and costly to prepare, while liquid cyclic luminescent reagents are unstable and difficult to fill and preserve quickly and accurately.

Method used

A liquid composition comprising luciferase, luciferin and related enzymes and cofactors was developed, and the enzyme concentration and pH were optimized by reducing the relative luminescence at storage to below 5500 RLU, and ATP-degrading enzymes or reaction inhibitors were added to improve stability.

Benefits of technology

This study improved the stability of liquid circulating luminescent reagents, simplified the preparation process, reduced costs, and ensured the accuracy and reliability of measuring ATP, AMP, and/or ADP in samples.

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Abstract

In one embodiment, the present invention addresses the problem of providing a liquid circulating luminescent reagent having excellent stability. In one embodiment, the present invention provides a liquid composition for use in determining ATP, and AMP and / or ADP in a sample after preservation: (i) the liquid composition comprises luciferase, fluorescein, an enzyme that catalyzes a reaction of producing ATP from AMP, a substrate that catalyzes an enzyme of a reaction of producing ATP from AMP, and a cofactor; and (ii) when the liquid composition does not contain at least one of these components, the component that is not contained in the liquid composition is added to the liquid composition before or during measurement, and when the liquid composition is stored, the relative luminescence amount of the liquid composition is 5500 RLU or less, said relative luminescence amount being a value obtained by subtracting the control value from the measured value.
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