Colistin monoclonal antibody, detection test strip and application thereof

By developing monoclonal antibodies and colloidal gold immunochromatographic test strips for colistin residue detection, the problems of incomplete antibody sequences and insufficient recognition balance in colistin residue detection have been solved, achieving rapid on-site detection with high sensitivity and specificity.

CN122541560BActive Publication Date: 2026-09-29XINUOTONGKE (TIANJIN) BIOTECHNOLOGY CO LTD +3
2 Cites 0 Cited by

Patent Information

Application Number
CN202610975802.7
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2026-07-02
Publication Date
2026-09-29
Estimated Expiration
2046-07-02

AI Technical Summary

Technical Problem

Existing colistin residue detection methods suffer from incomplete antibody sequence information, insufficient recognition uniformity, low sensitivity and specificity, and lack of rapid on-site detection methods, thus failing to meet the needs of large-scale sample screening.

Method used

A monoclonal antibody against colistin was developed by preparing monoclonal antibodies with well-defined amino acid and nucleotide sequences in the complementarity-determining regions (CDRs) of the light and heavy chain variable regions, and combining them with colloidal gold immunochromatographic test strips for rapid detection of colistin residues.

Benefits of technology

It achieves highly sensitive detection of colistin (IC50 of 1 ng/ml), with strong specificity (cross-reactivity rate <0.005%), suitable for rapid on-site screening and initial screening of large batches of samples, with a detection time of 5-10 minutes and visible results.

✦ Generated by Eureka AI based on patent content.
Patent Text Reader

Abstract

The application discloses a colistin monoclonal antibody, a detection test strip and application thereof, and belongs to the technical field of immune detection. The application immunizes mice with a colistin-BSA immunogen, and through cell fusion and screening, a hybridoma cell strain capable of stably secreting an anti-colistin monoclonal antibody is obtained. The heavy chain and light chain variable regions of the monoclonal antibody have specific complementarity determining region sequences, have very high sensitivity and specificity to colistin (polymyxin E) and polymyxin B, and have an IC50 value as low as 1 ng / mL, and have no cross reaction with common antibiotics such as norfloxacin. Based on the antibody, the application also develops a colloidal gold immunochromatography test strip. The test strip is simple to operate, rapid in detection, and visual in results, and can be used for on-site rapid screening of colistin residues in animal-derived food and biological samples, and has a good application prospect.
Need to check novelty before this filing date? Find Prior Art