KASP1 molecular marker for identifying resistance to soybean phytophthora and application thereof
CN122898666APending Publication Date: 2026-10-13SANYA INSTITUTE OF NANJING AGRICULTURAL UNIVERSITY +1
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Patent Information
- Application Number
- CN202611381848.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-09-08
- Publication Date
- 2026-10-13
AI Technical Summary
Technical Problem
[0004]传统的表型鉴定存在一定的局限性：耗时长，易受环境的影响，并且由于该菌的土传特性不能在大田中进行直接接种鉴定等缺点
Benefits of technology
[0022]1、在抗性基因附近开发紧密连锁的KASP1分子标记。该标记应用到高通量技术可以实现大豆的疫霉抗性高效率、快速的鉴定及基因分型。在大豆苗期就能够及早鉴别大豆疫霉抗性植株，具有高通量、低成本和高准确性的优势。克服了传统表型鉴定耗时长、易受环境因素影响、以及因大豆疫霉菌土传特性而无法在大田直接进行大规模接种鉴定的技术瓶颈，非常适合在常规育种实验室进行大规模商业化推广应用，为快速、精准地筛选抗疫霉根腐病大豆种质资源提供了强有力的工具。
✦ Generated by Eureka AI based on patent content.
Abstract
The application relates to the field of agricultural biotechnology, and particularly discloses a KASP1 molecular marker for identifying the resistance of soybeans to pythium root rot and application. The KASP1 molecular marker primer sequence is shown in the upstream primer SEQ ID No. 1 (connected with a FAM fluorescent tag), the upstream primer SEQ ID No. 2 (connected with a VIC fluorescent tag) and the universal downstream primer SEQ ID No. 3. The KASP1 molecular marker can realize efficient, accurate and low-cost identification and genotyping of the resistance of soybeans to pythium root rot, and the effective identification and detection reaches 96.10%, and the accuracy reaches 73.65%.
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