Preparation method of enzyme-cut small-molecule peptide nutrient solution and application thereof

Abstract

The application discloses a preparation method of an enzyme-cut small-molecule peptide nutrient solution and belongs to the technical field of biological enzymolysis. The method comprises the following steps: mixing, crushing and then adding an animal protein, a calcium source auxiliary material and molasses, adding a compound enzyme composed of proteolytic enzyme, bromelain and aspergillus for segmented enzyme addition, performing one-stage enzymolysis at 43-55 DEG C for 240-300 minutes, and then performing low-temperature fermentation and post-fermentation until the content of a peptide segment with a molecular weight less than 1000 Dalton reaches more than 90%, and finally performing four-stage gradient filtration and compounding to obtain the small-molecule peptide nutrient solution. The method improves the protein enzymolysis efficiency through temperature control enzymolysis. The obtained small-molecule peptide nutrient solution has good water solubility, can be compounded with nitrogen, phosphorus and potassium fertilizers to prepare compound fertilizers, and is suitable for increasing the yield of economic crops such as sugarcane.