Application of cotton GhFPL3 gene in regulating flowering time and cultivating early-maturing cotton

Abstract

This invention discloses cotton GhFPL3 The application of genes in regulating flowering time in plants and cultivating early-maturing cotton involves the field of plant genetic engineering technology. This invention cloned from upland cotton... GhFPL3 The gene, whose nucleotide sequence is shown in SEQ ID NO.1, was obtained by overexpression in Arabidopsis thaliana. GhFPL3 Genes showed that the bolting and flowering times of transgenic Arabidopsis thaliana were significantly earlier than those of the wild type, and salt tolerance was significantly enhanced; VIGS technology was used to silence endogenous genes in cotton. GhFPL3 The gene was silenced, and the flowering time of the silenced plant was significantly later than that of the control, and the key downstream genes of flowering were also silenced. GhLFY The expression level of was significantly downregulated. The results indicate that... GhFPL3 Genes positively regulate flowering time in cotton. This invention also relates to... GhFPL3 Application of genes in breeding early-maturing or late-maturing cotton varieties. This invention provides important genetic resources and theoretical basis for molecular breeding of early-maturing and salt-tolerant cotton traits.