An online solid-liquid separation and collection device

Abstract

The utility model discloses an online solid -liquid separation collection device, including clamp, feed liquid entrance, liquid inlet exhaust port, liquid outlet exhaust port and feed liquid export, when working, feed liquid enters the liquid inlet runner in the clamp from feed liquid entrance and fills the liquid inlet runner under the exhaust cooperation of liquid inlet exhaust port, after, the feed liquid in the liquid inlet runner passes through filter membrane and enters the liquid outlet runner and fills the liquid outlet runner under the exhaust cooperation of liquid outlet exhaust port, and the filtered feed liquid is finally discharged by feed liquid export. The utility model provides an online solid -liquid separation collection device, not only has reduced equipment cost and personnel cost greatly, and also has improved the homogeneity and yield of target product.

Description

Technical Field

[0001] This utility model relates to the field of biopharmaceutical equipment technology, specifically to an online solid-liquid separation and collection device for protein separation. Background Technology

[0002] In the pharmaceutical industry, solid-liquid separation of feed liquids and collection of the target solid phase are core steps in the drug production process, directly affecting the purity, yield, and final drug quality of the active ingredient. Whether it is chemically synthesized drugs, biological agents (such as proteins and vaccines), or nanomedicine carriers, efficient separation technologies are required to extract the target solid components (such as crystals, cell debris, microparticles, or nanoparticles) from complex liquid matrices (such as reaction solutions, fermentation broths, extracts, or crystallization mother liquors).

[0003] Currently, the existing technology for protein separation is through centrifuges. This method has the following drawbacks: 1. It requires a large number of centrifuges, resulting in high equipment procurement costs; 2. Each centrifuge requires personnel to operate, thus requiring a large number of personnel and incurring high labor costs; 3. The separation effect of each centrifuge varies, leading to inhomogeneity of the target product, and incomplete centrifugation can reduce the yield.

[0004] Therefore, a new type of equipment is needed to solve the problems of existing centrifuges for protein separation, such as the large number of centrifuges required for the same batch of production, the large number of operators required, the uneven separation of the liquid by different centrifuges, and the slightly lower yield of the target protein. Utility Model Content

[0005] The purpose of this invention is to provide an online solid-liquid separation and collection device that reduces equipment and labor costs and improves the uniformity and yield of the target product.

[0006] To achieve the above objectives, this utility model proposes an online solid-liquid separation and collection device, including a clamp, a liquid inlet, a liquid inlet vent, a liquid outlet vent, and a liquid outlet. The clamp includes a cavity formed therein and at least one filter membrane. The filter membrane divides the cavity into at least one liquid inlet channel and at least one liquid outlet channel. Each side of the filter membrane forms one liquid inlet channel and one liquid outlet channel, respectively. All liquid inlet channels in the cavity are connected to the liquid inlet and the liquid inlet vent, and all liquid outlet channels in the cavity are connected to the liquid outlet vent and the liquid outlet. The liquid enters each liquid inlet channel from the liquid inlet and fills the liquid inlet channel with the help of the venting of the liquid inlet vent. Then, the liquid in each liquid inlet channel passes through the filter membrane and enters the liquid outlet channel, filling the liquid outlet channel with the help of the venting of the liquid outlet vent. The filtered liquid is finally discharged from the liquid outlet.

[0007] In a preferred embodiment, one end of the liquid inlet channel is connected to the liquid inlet, and the other end is connected to the liquid inlet vent; one end of the liquid outlet channel is connected to the liquid outlet vent, and the other end is connected to the liquid outlet.

[0008] In a preferred embodiment, the directions of the liquid inlet, the liquid inlet vent, the liquid outlet vent, and the liquid outlet are all first directions, while the directions of the liquid inlet channel and the liquid outlet channel are second directions perpendicular to the first direction.

[0009] In a preferred embodiment, the liquid inlet and the liquid outlet are located at two opposite corners of a square, and the liquid outlet and the liquid outlet are located at the other two opposite corners of the square.

[0010] In a preferred embodiment, the device further includes two clamping plates located on both sides of the clamp, the clamp being clamped within the two clamping plates.

[0011] In a preferred embodiment, the device further includes reinforcing ribs, with one reinforcing rib fixed to the outer side of each clamping plate.

[0012] In a preferred embodiment, the device further includes a clamp locking assembly, which includes a locking lever and a hydraulic locking element. The locking lever and the hydraulic locking element are located outside the two clamping plates and are connected to each other, and are used to apply pressure to the clamping plates to lock the clamps.

[0013] In a preferred embodiment, the clamp includes multiple sets of stacked clamping plate assemblies, each set of clamping plate assemblies includes a filter membrane clamp, and a filter membrane is disposed between two adjacent filter membrane clamps.

[0014] In a preferred embodiment, a support plate is further provided between two adjacent filter membranes, and the two sides of the support plate respectively support the filter membranes on the corresponding sides.

[0015] In a preferred embodiment, a valve for controlling opening and closing is provided on each of the liquid inlet, liquid inlet vent, liquid outlet vent, and liquid outlet.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] This invention provides an online solid-liquid separation and collection device that uses a novel method—filtration through a filter membrane—to separate proteins from a protein feed solution. Compared to existing protein separation methods using centrifuges, this online solid-liquid separation and collection device allows for protein separation with a single unit, significantly reducing equipment costs (by approximately 70%). Furthermore, the device requires only one operator, greatly reducing labor costs (by approximately 70%–90%). In addition, the use of a filter membrane after the overall separation process improves uniformity and increases the yield of the target protein.

[0018] This invention, through the layout design of the internal flow channel structure of the clamp and the four interfaces on the device, ensures thorough filtration during the overall separation process, further improving the uniformity of the liquid and the yield of the target protein (by approximately 10%).

[0019] The clamp of this utility model adopts a multi-layer stacking design, and the number of stacking layers of the clamp components can be adjusted as needed, thereby meeting the filtration requirements of different liquid volumes.

[0020] This invention adds a pair of support plates between each pair of adjacent filter membranes to support the filter membranes, which improves the service life of the filter membranes, optimizes the material flow channel design, and also improves the recovery rate of the target protein. Attached Figure Description

[0021] Figure 1 , 3 5 and 6 are side view structural schematic diagrams of the clamp of this utility model;

[0022] Figure 2 for Figure 1 A sectional view;

[0023] Figure 4 for Figure 3 A cross-sectional view along the AA direction;

[0024] Figure 6 for Figure 5 A cross-sectional view along the BB direction.

[0025] The attached figures are labeled as follows:

[0026] 1. Fixture; 11. Fixture shelf assembly; 111. Filter membrane clamp; 12. Filter membrane; 13. Liquid outlet channel; 14. Liquid inlet channel; 15. Valve; 2. Feed inlet; 3. Liquid inlet vent; 4. Liquid outlet vent; 5. Feed outlet; 6. Fixture clamp; 7. Reinforcing rib; 8. Locking rod; 9. Hydraulic locking component; 10. Pull rod. Detailed Implementation

[0027] The specific embodiments of this utility model are described in detail below, but it should be understood that the scope of protection of this utility model is not limited to the specific embodiments.

[0028] Unless otherwise expressly stated, throughout the specification and claims, the term "comprising" or its variations such as "including" or "comprising" shall be understood to include the stated elements or components without excluding other elements or other components.

[0029] Combination Figures 1-6 As shown in the figure, an online solid-liquid separation and collection device disclosed in this embodiment of the present invention includes a clamp 1, a liquid inlet 2, a liquid inlet vent 3, a liquid outlet vent 4, and a liquid outlet 5. During operation, the liquid enters the liquid inlet channel within the clamp from the liquid inlet 2 and fills the liquid inlet channel with the assistance of the venting function of the liquid inlet vent 3. Then, the liquid in the liquid inlet channel passes through a filter membrane and enters the liquid outlet channel, where it fills the liquid outlet channel with the assistance of the venting function of the liquid outlet vent 4. The filtered liquid is finally discharged from the liquid outlet 5.

[0030] Specifically, in combination Figure 2 , 4 and Figure 6 As shown, the clamp 1 includes multiple sets of clamps arranged in a first direction (e.g., ...). Figure 2 The clamping plate assembly 11 (shown laterally) is a stacked arrangement of clamping plates. Each clamping plate assembly 11 includes a filter membrane clamping plate 111, with a filter membrane 12 disposed between two adjacent filter membrane clamping plates 111. After stacking, the two outermost filter membrane clamping plates 111 enclose the clamping space into a cavity. Each filter membrane 12 forms an outlet channel 13 and an inlet channel 14 on its left and right sides, respectively. That is, the cavity is divided by the filter membranes 12 within it into at least one inlet channel 14 and at least one outlet channel 13. The outlet channel 13 and the inlet channel 14 are arranged along a second direction perpendicular to the first direction (e.g., ...). Figure 2 (As shown longitudinally). All liquid inlet channels 14 within the cavity are connected to the liquid inlet 2 and the liquid inlet vent 3, and all liquid outlet channels within the cavity are connected to the liquid outlet vent 4 and the liquid outlet 5. In this embodiment, the fixture includes nine sets of fixture layer assemblies 11, with eight filter membranes 12 sandwiched between them. These eight filter membranes 12 divide the cavity within the fixture into eight liquid inlet channels 14 and eight liquid outlet channels 13. All eight liquid inlet channels 14 are connected to the liquid inlet 2 and the liquid inlet vent 3, and all eight liquid outlet channels 13 are connected to the liquid outlet vent 4 and the liquid outlet 5. Of course, the specific number of stacked layers of the fixture layer assemblies 11 can be set according to actual needs to meet the filtration requirements of different liquid volumes. In implementation, the fixture can be equipped with filter membranes 12 with a thickness of 4mm to 8mm, which can be flexibly replaced according to the filtration process requirements.

[0031] Preferably, a support plate 6 is provided between two adjacent filter membranes 12. Each support plate 6 supports the filter membrane 12 on the corresponding side on both sides, and also optimizes the flow channel design in the fixture.

[0032] In addition, the online solid-liquid separation and collection device disclosed in this embodiment of the present invention further includes two clamping plates 6, reinforcing ribs 7, and a clamping locking assembly. The two clamping plates 6 are respectively clamped on the left and right sides of the clamp. Preferably, a reinforcing rib 7 is fixed to the outer side of each clamping plate 6, which helps to improve the rigidity of the clamping plate 6. The clamping locking assembly specifically includes a locking rod 8 and a hydraulic locking component 9. The locking rod 8 and the hydraulic locking component 9 are located outside the two clamping plates 6 and are connected to each other, used to apply pressure to the clamping plates 6, thereby locking the clamping layer assembly inside the clamp. Furthermore, if necessary, a pull rod 10 can be added between the two clamping plates 6 to further apply clamping force to the two clamping plates 6, ensuring that the clamping layer assembly inside the clamp can be clamped together.

[0033] The aforementioned liquid inlet 2, liquid inlet vent 3, liquid outlet vent 4, and liquid outlet 5 are specifically disposed on one of the clamping plates 6. The liquid inlet 2 and liquid inlet vent 3 are respectively located at two opposite corners of the square inner side of the clamping plate 6; in this embodiment, they are located at the lower left and upper right corners of the clamping plate 6, respectively. The liquid outlet vent 4 and liquid outlet 5 are respectively located at the other two opposite corners of the square; in this embodiment, they are located at the upper left and lower right corners of the clamping plate 6, respectively. Furthermore, the directions of the liquid inlet 2, liquid inlet vent 3, liquid outlet vent 4, and liquid outlet 5 are all perpendicular to the first direction, i.e., they all extend laterally. The directions of the liquid inlet channel 14 and liquid outlet channel 13 are perpendicular to the first direction, i.e., they all extend longitudinally. Furthermore, one end of the liquid inlet channel 14 is connected to the liquid inlet 2, and the other end is connected to the liquid inlet vent 3; one end of the liquid outlet channel 13 is connected to the liquid outlet vent 4, and the other end is connected to the liquid outlet 5.

[0034] In addition, a valve 15 for controlling opening and closing is also provided on each of the above-mentioned liquid inlet 2, liquid inlet vent 3, liquid outlet vent 4 and liquid outlet 5.

[0035] During implementation, the fixture can meet the cleaning requirements of the process by enabling in-line cleaning (CIP) without any blind spots. Furthermore, the fixture can meet the sterilization requirements of the process by enabling in-line high-temperature steam sterilization (SIP), simultaneously sterilizing both the filter membrane and the fixture. Before use, the filter membrane needs to be rinsed: single-layer filter membranes should be rinsed with 25-50 L / m² of water at 1.3 times the standard flow rate, and double-layer filter membranes should be rinsed with 50-100 L / m² of water at 1.3 times the standard flow rate.

[0036] The specific working principle of this utility model fixture is as follows: Liquid enters the liquid inlet channel 14 inside the fixture from the liquid inlet 2. At this time, the liquid inlet vent 3 is opened to vent the liquid inlet channel 14, allowing the liquid to fill the liquid inlet channel 14. After the liquid is full, the liquid inlet vent 3 is closed through the valve 15. Figure 4 As shown. The feed liquid passes through the filter membrane 12 and enters the outlet channel 13. At this time, the outlet vent 4 is opened to vent the outlet channel 13, allowing the feed liquid to fill the outlet channel 13. After the channel is full, the outlet vent 4 is closed, and the filtered feed liquid is finally discharged from the feed outlet 5. Figure 6 As shown.

[0037] The advantages of this utility model are as follows: 1. This utility model provides an online solid-liquid separation and collection device, which achieves the separation of protein in protein feed liquid through a novel method of filtration separation using a filter membrane. Compared to existing protein separation methods using centrifuges, this online solid-liquid separation and collection device allows for protein separation with a single unit, significantly reducing equipment costs (by approximately 70%). Furthermore, the device requires only one operator, drastically reducing labor costs (by approximately 70%–90%). Additionally, filtration using a membrane after separation improves uniformity and increases the yield of the target protein. 2. The design of the internal flow channel structure of the clamp and the layout of the four interfaces on the device ensures thorough filtration during the separation process, further improving the uniformity of the liquid and the yield of the target protein (by approximately 10%). 3. The clamp of this invention employs a multi-layer stacking design, allowing adjustment of the stacking layer to accommodate different liquid volumes. 4. This invention adds a pair of support plates between each pair of adjacent membranes, improving membrane lifespan, optimizing the liquid flow channel design, and increasing the recovery rate of the target protein.

[0038] The foregoing description of specific exemplary embodiments of the present invention is for illustrative and explanatory purposes. These descriptions are not intended to limit the present invention to the precise forms disclosed, and it will be apparent that many changes and variations can be made in accordance with the foregoing teachings. The exemplary embodiments were chosen and described in order to explain the specific principles of the present invention and its practical application, thereby enabling those skilled in the art to implement and utilize various different exemplary embodiments of the present invention, as well as various different choices and variations. The scope of the present invention is intended to be defined by the claims and their equivalents.

Claims

1. An in-line solid-liquid separation collection device, characterized by, The device includes a clamp, a liquid inlet, a liquid inlet vent, a liquid outlet vent, and a liquid outlet. The clamp includes a cavity formed therein and at least one filter membrane. The filter membrane divides the cavity into at least one liquid inlet channel and at least one liquid outlet channel. Each side of the filter membrane forms one liquid inlet channel and one liquid outlet channel, respectively. All liquid inlet channels in the cavity are connected to the liquid inlet and the liquid inlet vent, and all liquid outlet channels in the cavity are connected to the liquid outlet vent and the liquid outlet. The liquid enters each liquid inlet channel from the liquid inlet and fills the liquid inlet channel with the help of the venting of the liquid inlet vent. Then, the liquid in each liquid inlet channel passes through the filter membrane and enters the liquid outlet channel, filling the liquid outlet channel with the help of the venting of the liquid outlet vent. The filtered liquid is finally discharged from the liquid outlet.

2. An in-line solid-liquid separation and collection device as claimed in claim 1, wherein, One end of the liquid inlet channel is connected to the liquid inlet, and the other end is connected to the liquid inlet vent. One end of the liquid outlet channel is connected to the liquid outlet vent, and the other end is connected to the liquid outlet.

3. An in-line solid-liquid separation and collection device as claimed in claim 2, wherein, The directions of the liquid inlet, liquid inlet vent, liquid outlet vent, and liquid outlet are all the first direction, while the directions of the liquid inlet channel and liquid outlet channel are the second direction, which is perpendicular to the first direction.

4. An in-line solid-liquid separation and collection device as claimed in claim 3, wherein, The liquid inlet and the liquid outlet are located at two opposite corners of a square, while the liquid outlet and the liquid outlet are located at the other two opposite corners of the square.

5. An in-line solid-liquid separation and collection device as claimed in claim 1, wherein, The device also includes two clamping plates located on both sides of the clamp, and the clamp is clamped within the two clamping plates.

6. An in-line solid-liquid separation and collection device as claimed in claim 5, wherein, The device also includes reinforcing ribs, with one reinforcing rib fixed to the outer side of each clamping plate.

7. An in-line solid-liquid separation and collection device as claimed in claim 6, wherein, The device also includes a clamp locking assembly, which includes a locking rod and a hydraulic locking component. The locking rod and the hydraulic locking component are located outside the two clamping plates and are connected to each other, and are used to apply pressure to the clamping plates to lock the clamps.

8. An in-line solid-liquid separation and collection device as claimed in any one of claims 1 to 7, wherein, The fixture includes multiple sets of stacked fixture plate assemblies, each set of fixture plate assemblies includes a filter membrane clamp, and a filter membrane is disposed between two adjacent filter membrane clamps.

9. An in-line solid-liquid separation and collection device as claimed in claim 8, wherein, A support plate is also provided between two adjacent filter membranes, and the two sides of the support plate support the filter membranes on the corresponding sides respectively.

10. An in-line solid-liquid separation and collection device as claimed in claim 1, wherein, Each of the following is equipped with a valve for controlling its opening and closing: liquid inlet, liquid outlet, liquid outlet, and liquid outlet.

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