Imaging analysis system

Abstract

This invention provides an imaging analysis system, comprising: a housing; a sample stage mounted on the housing, the upper surface of the sample stage having a mounting portion for placing a culture unit, and the sample stage also having a vertically penetrating channel located within the area of ​​the mounting portion; and an imaging module located below the sample stage and connected to the lower surface of the sample stage, the imaging module being used to photograph the culture unit through the channel. By placing the mounting portion for placing the culture unit on the upper surface of the sample stage and connecting the imaging module to the lower surface of the sample stage, the imaging module can photograph the culture unit from below through the channel on the sample stage, thus obtaining a clear image of the culture unit. This simplifies the system structure, reduces the system size, and allows the culture unit and imaging module to be mounted on the same component (sample stage), reducing the machining and assembly precision requirements of the components, while also ensuring clear imaging by the imaging module.

Description

Technical Field

[0001] This utility model relates to the field of experimental instrument technology, and in particular to an imaging analysis system. Background Technology

[0002] In the life sciences, microscopic fluorescence imaging of biological samples allows for the assessment of various characteristics, such as growth, morphology, and drug response, at one or more time points. Existing imaging analysis systems consist of an imaging module and a sample stage. The imaging module is mounted on one support plate, while the sample stage is positioned on another. This arrangement results in the sample and imaging module being located on separate supports. Ensuring clear imaging of the sample across the entire sample stage places higher demands on the machining and assembly precision of the two support plates, complicating the fabrication and assembly of the imaging subsystem. However, existing imaging systems struggle to meet these high requirements, leading to poor image quality. Utility Model Content

[0003] The technical problem to be solved by this utility model is to overcome the defects of the prior art and provide an imaging analysis system.

[0004] The present invention solves the above-mentioned technical problems through the following technical solution:

[0005] An imaging analysis system comprising:

[0006] case;

[0007] A sample stage is mounted on the housing. The upper surface of the sample stage has a mounting portion for placing a culture unit. The sample stage also has a vertically penetrating channel located within the area of ​​the mounting portion.

[0008] An imaging module is located below the sample stage and connected to the lower surface of the sample stage. The imaging module is used to photograph the culture unit through the channel.

[0009] In this solution, by changing the layout and connection relationship of the imaging module relative to the sample stage in the imaging analysis system, the mounting part for placing the culture unit is located on the upper surface of the sample stage, and the imaging module is connected to the lower surface of the sample stage. This allows the culture unit and the imaging module to be located on opposite sides of the sample stage. The imaging module captures a clear image of the culture unit from below through a channel on the sample stage, avoiding the imaging module occupying space above the sample stage and facilitating the placement and removal of the culture unit relative to the mounting part. Simultaneously, by connecting the lower imaging module to the sample stage, the system structure is simplified, and the system size is reduced. Since both the culture unit and the imaging module are mounted on the same component (the sample stage), the machining and assembly precision requirements of the components are reduced, while still ensuring clear imaging of the culture unit.

[0010] Preferably, the imaging analysis system further includes a first driving unit, a first guiding unit, and a first connector. The first connector is connected to the imaging module and to the sample stage via the first guiding unit. The first guiding unit extends along a first direction. The first driving unit is connected to the imaging module or the first connector and is used to drive the imaging module to slide along the first direction.

[0011] In this design, the imaging module is connected to the first connector, and the first connector is connected to the sample stage via the first guide unit, thus fixing the imaging module on the sample stage. Since the first guide unit extends along a first direction, when the first drive unit drives the imaging module to move, the imaging module slides along the first direction under the guidance of the first guide unit, allowing the imaging module to capture images of the culture unit at different positions.

[0012] Since the imaging module is connected to the first connector, the first driving unit can also achieve the function of the imaging module sliding along the first direction by connecting and driving the first connector or the imaging module.

[0013] Preferably, the first guide unit includes a first slide groove and a first slide rail. The first slide rail is connected to the lower surface of the sample stage and extends along the first direction. The first slide groove is disposed on the upper surface of the first connector and is sleeved on the first slide rail.

[0014] Alternatively, the first drive unit includes a first motor and a first transmission lead screw, the first motor is mounted on the sample stage, the output shaft of the first motor is connected to the first transmission lead screw, and the first transmission lead screw is threadedly connected to the imaging module.

[0015] In this solution, the guide structure formed by the first slide groove and the first slide rail is simple in structure and easy to install. Of course, in other alternative solutions, the first guide unit may include a guide rod and a slider slidably mounted on the guide rod to replace the guide structure of the slide groove and the slide rail, which can also achieve the guiding function.

[0016] Since the first transmission lead screw is threadedly connected to the imaging module, the first motor drives the first transmission lead screw to rotate. When the first transmission lead screw rotates, the imaging module slides along the first direction under the drive of the transmission lead screw. The cooperation between the first motor, the first transmission lead screw, and the imaging module ensures smooth transmission, improves transmission accuracy, and facilitates connection with the first motor via a control board, enabling precise control of the imaging module's movement direction and distance.

[0017] Preferably, the imaging analysis system further includes a second driving unit and a second guiding unit. The imaging module includes an imaging module and a second connector. The second connector is connected to the imaging module and is connected to the first connector via the second guiding unit. The second guiding unit extends along a second direction. The second driving unit is connected to the imaging module and is used to drive the imaging module to slide along a second direction different from the first direction.

[0018] In this design, the imaging module is connected to a second connector, which is connected to a first connector via a second guide unit. The first connector is connected to the sample stage via a first guide unit, thus fixing the imaging module on the sample stage. Since the second guide unit extends along a second direction, when the second drive unit moves the imaging module, the module slides along the second direction under the guidance of the second guide unit, allowing the imaging module to capture images of the culture unit at different positions.

[0019] Preferably, the second guide unit includes a second slide groove and a second slide rail. The second slide rail is connected to the lower surface of the first connector and extends along the second direction. The second slide groove is disposed on the upper surface of the second connector and is sleeved on the second slide rail.

[0020] Alternatively, the second drive unit includes a second motor and a second transmission screw, the second motor is mounted on the first connector, the output shaft of the second motor is connected to the second transmission screw, and the second transmission screw is threadedly connected to the imaging module.

[0021] In this design, the guide structure formed by the second slide groove and the second slide rail is simple in structure and easy to install. Of course, in other alternative designs, the second guide unit may include a guide rod and a slider slidably mounted on the guide rod to replace the guide structure consisting of the slide groove and the slide rail, achieving the same guiding function.

[0022] Because the second transmission lead screw is threadedly connected to the imaging module, the second motor drives the second transmission lead screw to rotate. When the second transmission lead screw rotates, the imaging module slides along the second direction under the drive of the transmission lead screw. The cooperation between the second motor, the second transmission lead screw, and the imaging module ensures smooth transmission, improves transmission accuracy, and facilitates connection with the second motor via a control board, enabling precise control of the imaging module's movement direction and distance.

[0023] Preferably, the imaging analysis system further includes a gantry and a light source module. The legs of the gantry pass through the clearance space on the sample stage and are connected to the first connector. The light source module is mounted on the crossbeam of the gantry and is used to irradiate the culture unit.

[0024] In this design, when the light source module is activated, it illuminates the culture unit, facilitating clear imaging of the culture unit on the imaging module. The gantry is used to mount and fix the light source module. A clearance space is provided on the sample stage, through which the gantry's legs connect to the first connector, preventing interference between the gantry's legs and the sample stage, thus making the entire system compact. The clearance space can be any type of hole or notch on the sample stage.

[0025] Since the first connector is connected to the sample stage through the first guide unit, when the first drive unit drives the imaging module to move, the imaging module and the first connector slide along the first direction under the guidance of the first guide unit. At the same time, the gantry also moves with the first connector (the legs of the gantry are connected to the first connector), realizing the synchronous movement of the imaging module and the light source module, which facilitates rapid imaging of the culture unit at different positions.

[0026] Preferably, the imaging analysis system further includes a third driving unit and a third guiding unit. The light source module is mounted on the crossbeam of the gantry via the third guiding unit, which extends along the length of the crossbeam. The third driving unit is connected to the light source module and is used to drive the light source module to slide along the length of the crossbeam.

[0027] In this design, the light source module is mounted on the crossbeam of the gantry via a third guide unit. When the third drive unit drives the light source module to slide along the length of the crossbeam, the light source module slides along the length of the crossbeam under the guidance of the third guide unit, facilitating the adjustment of the light source module's position to provide better illumination to the culture unit and lay the foundation for clear imaging by the imaging module.

[0028] Preferably, the third guide unit includes a third slide groove and a third slide rail. The third slide groove is connected to the crossbeam of the gantry and extends along the length of the crossbeam. The third slide rail is disposed on the light source module, and the third slide groove is sleeved on the third slide rail.

[0029] Alternatively, the third drive unit includes a third motor and a third transmission screw, the third motor is mounted on the gantry, the output shaft of the third motor is connected to the third transmission screw, and the third transmission screw is threadedly connected to the light source module.

[0030] In this design, the guide structure formed by the third slide groove and the third slide rail is simple in structure and easy to install. Of course, in other alternative designs, the third guide unit may include a guide rod and a slider slidably mounted on the guide rod to replace the guide structure of the slide groove and slide rail, which can also achieve the guiding function.

[0031] Because the third transmission screw is threadedly connected to the light source module, the third motor drives the third transmission screw to rotate. When the third transmission screw rotates, the light source module slides along the length of the crossbeam under the drive of the transmission screw. The cooperation between the third motor, the third transmission screw, and the light source module ensures smooth transmission, improves transmission accuracy, and facilitates connection with the third motor via the control board, enabling precise control of the light source module's movement direction and distance.

[0032] Preferably, the gantry includes two legs, both of which can be raised or lowered synchronously via a lifting mechanism.

[0033] In this solution, the height of the light source module installed on the crossbeam of the gantry can be adjusted simultaneously by raising or lowering the two legs of the gantry through the lifting mechanism. This can accommodate consumables of different sizes and improve the problem of concave liquid surface imaging in orifice plates.

[0034] Preferably, the sample stage is provided with a marker point, which serves as a reference zero point for each movement of the driving mechanism during continuous experiments.

[0035] In this scheme, before the drive mechanism is driven, the marked point can provide a reference zero point for the drive mechanism, which facilitates the alignment of the drive mechanism in multiple consecutive drives and solves the inherent deviation of the photoelectric switch zero position.

[0036] Based on common knowledge in the field, the above-mentioned preferred conditions can be combined arbitrarily to obtain various preferred embodiments of this utility model.

[0037] The significant advantages of this invention are as follows: By altering the layout and connection of the imaging module relative to the sample stage in the imaging analysis system, the mounting portion for placing the culture unit is positioned on the upper surface of the sample stage, while the imaging module is connected to the lower surface. This allows the culture unit and the imaging module to be located on opposite sides of the sample stage. The imaging module then captures a clear image of the culture unit from below through a channel on the sample stage, avoiding the imaging module occupying space above the sample stage and facilitating the placement and removal of the culture unit relative to the mounting portion. Furthermore, by connecting the lower imaging module to the sample stage, the system structure is simplified, and the system size is reduced. Since both the culture unit and the imaging module are mounted on the same component (the sample stage), the machining and assembly precision requirements of the components are lowered, while still ensuring clear imaging of the culture unit. Attached Figure Description

[0038] Figure 1 This is a schematic diagram of the imaging analysis system of Embodiment 1 of the present invention. Figure 1 .

[0039] Figure 2 for Figure 1 Cross-sectional view along line AA.

[0040] Figure 3 This is a schematic diagram of the imaging analysis system of Embodiment 1 of the present invention. Figure 2 .

[0041] Figure 4 for Figure 3 Cross-sectional view along line BB.

[0042] Figure 5 for Figure 3 Cross-sectional view along the CC line.

[0043] Figure 6 for Figure 3 Cross-sectional view along the DD line.

[0044] Figure 7 This is a schematic diagram of the sample stage in Embodiment 1 of this utility model.

[0045] Casing 1

[0046] Receiving cavity 11

[0047] Sample stage 2

[0048] Installation Department 21

[0049] Channel 211

[0050] Limiting step 212

[0051] Imaging Module 3

[0052] Imaging module 31

[0053] Second connector 32

[0054] First driving unit 4

[0055] First motor 41

[0056] First transmission screw 42

[0057] First guiding unit 5

[0058] First chute 51

[0059] First slide rail 52

[0060] First connector 6

[0061] Second drive unit 7

[0062] Second motor 71

[0063] Second transmission screw 72

[0064] Second guiding unit 8

[0065] Second slide 81

[0066] Second slide rail 82

[0067] Gantry Frame 9

[0068] 91 crossbeam

[0069] 92 feet

[0070] Light source module 10

[0071] Third drive unit 101

[0072] Third motor 1011

[0073] Third transmission lead screw 1012

[0074] Third guiding unit 102

[0075] Third chute 1021

[0076] Third slide rail 1022

[0077] First direction 100

[0078] Second direction 200 Detailed Implementation

[0079] The present invention will be described more clearly and completely below with reference to the accompanying drawings, using a preferred embodiment.

[0080] Example 1

[0081] like Figures 1-7 As shown, this embodiment discloses an imaging analysis system, which includes a housing 1, a sample stage 2, and an imaging module 3. The sample stage 2 is mounted on the housing 1, and its upper surface has a mounting portion 21 for placing a culture unit. The sample stage 2 also has a vertically penetrating channel 211 located within the area of ​​the mounting portion 21. The imaging module 3 is located below the sample stage 2 and connected to its lower surface. The imaging module 3 is used to capture images of the culture unit through the channel 211. The culture unit is used to culture biological samples, typically a petri dish or culture flask.

[0082] like Figures 1-7 As shown, in this embodiment, by changing the layout and connection relationship of the imaging module 3 relative to the sample stage 2 in the imaging analysis system, the mounting part 21 for placing the culture unit is set on the upper surface of the sample stage 2, and the imaging module 3 is connected to the lower surface of the sample stage 2, so that the culture unit and the imaging module 3 are located on opposite sides of the sample stage 2. The imaging module 3 can capture images of the culture unit from below through the channel 211 on the sample stage 2, thus obtaining a clear image of the culture unit. This avoids the imaging module 3 occupying the space above the sample stage 2, and facilitates the placement and removal of the culture unit relative to the mounting part 21 of the sample stage 2. At the same time, by connecting the imaging module 3 located below to the sample stage 2, the structure of the system is simplified and the volume of the system is reduced. The culture unit and the imaging module 3 are both set on the same part (sample stage 2), which reduces the machining accuracy and assembly accuracy of the parts, and also enables the culture unit to be clearly imaged.

[0083] like Figures 3-6 As shown, to enable the imaging module 3 to capture images of the culture unit at different positions, the imaging module 3, in addition to being connected to the lower surface of the sample stage 2, can also move relative to the sample stage 2. In this embodiment, to achieve the purpose of driving the imaging module 3 to move and adjust its position relative to the sample stage 2, the imaging analysis system further includes a first driving unit 4, a first guiding unit 5, and a first connecting member 6. The first connecting member 6 is connected to the imaging module 3 and is connected to the sample stage 2 through the first guiding unit 5. The first guiding unit 5 extends along the first direction 100. The first driving unit 4 is connected to the imaging module 3 and is used to drive the imaging module 3 to slide along the first direction 100. Since the imaging module 3 is connected to the first connecting member 6, and the first connecting member 6 is connected to the sample stage 2 through the first guiding unit 5, the imaging module 3 is fixed on the sample stage 2. Since the first guide unit 5 extends along the first direction 100, when the first drive unit 4 drives the imaging module 3 to move, the imaging module 3 slides along the first direction 100 under the guidance of the first guide unit 5, so that the imaging module 3 can capture images of the culture unit at different positions.

[0084] In another embodiment, since the imaging module 3 is connected to the first connector 6, the first driving unit 4 can also achieve the function of sliding the imaging module 3 along the first direction 100 by connecting to and driving the first connector 6.

[0085] like Figure 4 As shown, the first guide unit 5 includes a first groove 51 and a first slide rail 52. The first slide rail 52 is connected to the lower surface of the sample stage 2 and extends along the first direction 100. The first groove 51 is disposed on the upper surface of the first connector 6 and is fitted onto the first slide rail 52. The guide structure formed by the first groove 51 and the first slide rail 52 is simple in structure and easy to install. Of course, in other alternative embodiments, the first guide unit may include a guide rod and a slider sliding on the guide rod to replace the guide structure of the groove and the slide rail, which can also achieve the guide function.

[0086] like Figure 4 As shown, in this embodiment, in order to improve the guiding effect, there are two first guiding units 5, and the two first guiding units 5 are arranged at intervals between the sample stage 2 and the imaging module 3.

[0087] like Figure 4 As shown, the first drive unit 4 includes a first motor 41 and a first transmission screw 42. The first motor 41 is mounted on the sample stage 2, and the output shaft of the first motor 41 is connected to the first transmission screw 42. The first transmission screw 42 is threadedly connected to the imaging module 3. Since the first transmission screw 42 is threadedly connected to the imaging module 3, the first motor 41 drives the first transmission screw 42 to rotate. When the first transmission screw 42 rotates, the imaging module 3 slides along the first direction 100 under the drive of the transmission screw. The first motor 41, the first transmission screw 42, and the imaging module 3 cooperate to achieve smooth transmission, improve transmission accuracy, and facilitate connection with the first motor 41 via a control board to achieve precise control of the moving direction and moving distance of the imaging module 3.

[0088] like Figure 4 and Figure 5As shown, the imaging analysis system also includes a second driving unit 7 and a second guiding unit 8. The imaging module 3 includes an imaging module 31 and a second connector 32. The second connector 32 is connected to the imaging module 31 and is connected to the first connector 6 via the second guiding unit 8. The second guiding unit 8 extends along a second direction 200. The second driving unit 7 is connected to the imaging module 3 and is used to drive the imaging module 3 to slide along a second direction 200 different from the first direction 100. The imaging module 31 is connected to the second connector 32, which is connected to the first connector 6 via the second guiding unit 8. The first connector 6 is connected to the sample stage 2 via the first guiding unit 5, thus fixing the imaging module 31 to the sample stage 2. Since the second guiding unit 8 extends along the second direction 200, when the second driving unit 7 drives the imaging module 31 to move, the imaging module 31 slides along the second direction 200 under the guidance of the second guiding unit 8, so that the imaging module 31 can capture images of the culture unit at different positions.

[0089] like Figure 4 and Figure 5 As shown, the second guide unit 8 includes a second slide groove 81 and a second slide rail 82. The second slide rail 82 is connected to the lower surface of the first connecting member 6 and extends along the second direction 200. The second slide groove 81 is disposed on the upper surface of the second connecting member 32 and is fitted onto the second slide rail 82. The guide structure formed by the cooperation of the second slide groove 81 and the second slide rail 82 has a simple structure and is easy to install. Of course, in other alternative embodiments, the second guide unit may include a guide rod and a slider sliding on the guide rod to replace the guide structure of the slide groove and the slide rail, which can also achieve the guide function.

[0090] like Figure 4 As shown, in this embodiment, in order to improve the guiding effect, there are two second guide units 8, and the two second guide units 8 are arranged at intervals between the first connector 6 and the second connector 32.

[0091] In this embodiment, the imaging module 31 is used for imaging. The imaging module 31 includes 1-4 optional fluorescence channels 211 and one or more optional imaging magnifications (typically 7.5x, 10x, 15x), which makes the product highly integrated and small in size, and convenient for biofluorescence imaging and other application imaging and analysis.

[0092] For example, a three-channel fluorescence imaging module includes at least a three-channel fluorescence module, a three-channel dichroic mirror, a three-channel emission filter, an imaging lens group, an objective lens, and a camera. The objective lens and camera are optional; one, both, or neither can be present. The three-channel fluorescence module includes at least three fluorescence channel components, a necessary beam splitter, and a reflector or reflection device. Each fluorescence channel component includes at least: a monochromatic LED light source (or laser), a condenser lens group, and an excitation filter. The emitted light from the three-channel fluorescence module passes through an exit aperture to the three-channel dichroic mirror, is reflected through the objective lens, and illuminates the sample to excite a fluorescence signal. The signal is collected by the objective lens, passes through the three-channel dichroic mirror and the three-channel reflection filter, and is imaged onto an image sensor through the imaging lens or tube lens. The beam splitter is used for 45° reflection and transmission of light. The positional order of the monochromatic LEDs is not fixed.

[0093] like Figure 3 As shown, in this embodiment, the first direction 100 is longitudinal, and the second direction 200 is transverse. Of course, in other embodiments, the first and second directions can be set to other directions according to the user.

[0094] like Figure 4 and Figure 5 As shown, the second drive unit 7 includes a second motor 71 and a second transmission screw 72. The second motor 71 is mounted on the first connector 6, and the output shaft of the second motor 71 is connected to the second transmission screw 72. The second transmission screw 72 is threadedly connected to the imaging module 31. Since the second transmission screw 72 is threadedly connected to the imaging module 31, the second motor 71 drives the second transmission screw 72 to rotate. When the second transmission screw 72 rotates, the imaging module 31 slides along the second direction 200 under the drive of the transmission screw. The second motor 71, the second transmission screw 72, and the imaging module 31 cooperate to achieve smooth transmission, improve transmission accuracy, and facilitate connection with the second motor 71 via a control board to achieve precise control of the moving direction and moving distance of the imaging module 31.

[0095] like Figures 1-6 As shown, the imaging analysis system also includes a gantry 9 and a light source module 10. The legs 92 of the gantry 9 pass through the clearance space on the sample stage 2 and connect to the first connector 6. The light source module 10 is mounted on the crossbeam 91 of the gantry 9 and is used to irradiate the culture unit. When the light source module 10 is turned on, it illuminates the culture unit, facilitating clear imaging of the culture unit on the imaging module 3. The gantry 9 is used to mount and fix the light source module 10. The sample stage 2 has a clearance space, through which the legs 92 of the gantry 9 connect to the first connector 6, preventing interference between the legs 92 and the sample stage 2, thus making the entire system structure compact. The clearance space can be any type of hole or notch on the sample stage 2.

[0096] like Figures 1-6 As shown, in this embodiment, since the first connector 6 is connected to the sample stage 2 through the first guide unit 5, when the first drive unit 4 drives the imaging module 3 to move, the imaging module 3 and the first connector 6 slide along the first direction 100 under the guidance of the first guide unit 5. At the same time, the gantry 9 also moves with the first connector 6 (the legs 92 of the gantry 9 are connected to the first connector 6), realizing the synchronous movement of the imaging module 3 and the light source module 10, which facilitates rapid and clear imaging of the culture unit at different positions.

[0097] like Figure 5 and Figure 6 As shown, the imaging analysis system also includes a third drive unit 101 and a third guide unit 102. The light source module 10 is mounted on the crossbeam 91 of the gantry 9 via the third guide unit 102, which extends along the length of the crossbeam 91. The third drive unit 101 is connected to the light source module 10 and is used to drive the light source module 10 to slide along the length of the crossbeam 91. The light source module 10 is mounted on the crossbeam 91 of the gantry 9 via the third guide unit 102. When the third drive unit 101 drives the light source module 10 to slide along the length of the crossbeam 91, the light source module 10 slides along the length of the crossbeam 91 under the guidance of the third guide unit 102, which facilitates the adjustment of the position of the light source module 10 to provide better illumination to the culture unit, laying the foundation for clear imaging by the imaging module 31.

[0098] like Figure 5 and Figure 6 As shown, the third guide unit 102 includes a third slide groove 1021 and a third slide rail 1022. The third slide groove 1021 is connected to the crossbeam 91 of the gantry 9 and extends along the length of the crossbeam 91. The third slide rail 1022 is disposed on the light source module 10, and the third slide groove 1021 is fitted onto the third slide rail 1022. The guide structure formed by the cooperation of the third slide groove 1021 and the third slide rail 1022 is simple in structure and easy to install. Of course, in other alternative embodiments, the third guide unit may include a guide rod and a slider sliding on the guide rod to replace the guide structure of the slide groove and the slide rail, which can also achieve the guide function.

[0099] like Figure 5 and Figure 6As shown, the third drive unit 101 includes a third motor 1011 and a third transmission screw 1012. The third motor 1011 is mounted on the gantry 9, and the output shaft of the third motor 1011 is connected to the third transmission screw 1012. The third transmission screw 1012 is threadedly connected to the light source module 10. Since the third transmission screw 1012 is threadedly connected to the light source module 10, the third motor 1011 drives the third transmission screw 1012 to rotate. When the third transmission screw 1012 rotates, the light source module 10 slides along the length direction of the crossbeam 91 under the drive of the transmission screw. The cooperation between the third motor 1011, the third transmission screw 1012, and the light source module 10 ensures smooth transmission, improves transmission accuracy, and facilitates connection with the third motor 1011 via a control board, enabling precise control of the movement direction and distance of the light source module 10.

[0100] The sample stage 2 is equipped with marker points (not shown in the figure). These marker points serve as the reference zero point for each movement of the drive mechanism during continuous experiments. Before the drive mechanism is activated, the marker points provide a reference zero point, facilitating alignment during multiple consecutive drives and addressing the inherent deviation of the photoelectric switch's zero position.

[0101] like Figure 7 As shown, the mounting section 21 includes a rectangular limiting step 212, which facilitates the direct fixing of the rectangular culture unit onto the sample stage 2 without the need for an adapter. Microplates and T75 culture flasks can be placed directly on the limiting step without an adapter.

[0102] like Figures 1-7 As shown, the housing 1 has a receiving cavity 11 with an opening at the top, the sample stage 2 closes the opening, and the imaging module 3 is located in the receiving cavity 11.

[0103] Example 2

[0104] This embodiment is basically the same as Embodiment 1, except that the gantry includes two legs, both of which can be raised or lowered synchronously via a lifting mechanism. By raising or lowering the two legs of the gantry through the lifting mechanism, the height of the light source module mounted on the crossbeam of the gantry can be adjusted synchronously, which can accommodate consumables of different sizes and improve the problem of concave liquid surface imaging in orifice plates.

Claims

1. An imaging analysis system, characterized in that, It includes: case; A sample stage is mounted on the housing. The upper surface of the sample stage has a mounting portion for placing a culture unit. The sample stage also has a vertically penetrating channel located within the area of ​​the mounting portion. An imaging module is located below the sample stage and connected to the lower surface of the sample stage. The imaging module is used to photograph the culture unit through the channel.

2. The imaging analysis system as described in claim 1, characterized in that, The imaging analysis system further includes a first driving unit, a first guiding unit, and a first connector. The first connector is connected to the imaging module and is connected to the sample stage via the first guiding unit. The first guiding unit extends along a first direction. The first driving unit is connected to the imaging module or the first connector and is used to drive the imaging module to slide along the first direction.

3. The imaging analysis system as described in claim 2, characterized in that, The first guide unit includes a first slide groove and a first slide rail. The first slide rail is connected to the lower surface of the sample stage and extends along the first direction. The first slide groove is disposed on the upper surface of the first connector and is sleeved on the first slide rail. Alternatively, the first drive unit includes a first motor and a first transmission lead screw, the first motor is mounted on the sample stage, the output shaft of the first motor is connected to the first transmission lead screw, and the first transmission lead screw is threadedly connected to the imaging module.

4. The imaging analysis system as described in claim 2, characterized in that, The imaging analysis system further includes a second driving unit and a second guiding unit. The imaging module includes an imaging module and a second connector. The second connector is connected to the imaging module and is connected to the first connector via the second guiding unit. The second guiding unit extends along a second direction. The second driving unit is connected to the imaging module and is used to drive the imaging module to slide along a second direction different from the first direction.

5. The imaging analysis system as described in claim 4, characterized in that, The second guide unit includes a second slide groove and a second slide rail. The second slide rail is connected to the lower surface of the first connector and extends along the second direction. The second slide groove is disposed on the upper surface of the second connector and is sleeved on the second slide rail. Alternatively, the second drive unit includes a second motor and a second transmission screw, the second motor is mounted on the first connector, the output shaft of the second motor is connected to the second transmission screw, and the second transmission screw is threadedly connected to the imaging module.

6. The imaging analysis system as described in claim 2, characterized in that, The imaging analysis system also includes a gantry and a light source module. The legs of the gantry pass through the clearance space on the sample stage and are connected to the first connector. The light source module is mounted on the crossbeam of the gantry and is used to irradiate the culture unit.

7. The imaging analysis system as described in claim 6, characterized in that, The imaging analysis system further includes a third driving unit and a third guiding unit. The light source module is mounted on the crossbeam of the gantry via the third guiding unit, which extends along the length of the crossbeam. The third driving unit is connected to the light source module and is used to drive the light source module to slide along the length of the crossbeam.

8. The imaging analysis system as described in claim 7, characterized in that, The third guide unit includes a third slide groove and a third slide rail. The third slide groove is connected to the crossbeam of the gantry and extends along the length of the crossbeam. The third slide rail is disposed on the light source module, and the third slide groove is sleeved on the third slide rail. Alternatively, the third drive unit includes a third motor and a third transmission screw, the third motor is mounted on the gantry, the output shaft of the third motor is connected to the third transmission screw, and the third transmission screw is threadedly connected to the light source module.

9. The imaging analysis system as described in claim 6, characterized in that, The gantry frame includes two legs, both of which can be raised or lowered synchronously via a lifting mechanism.

10. The imaging analysis system as described in claim 1, characterized in that, The sample stage is provided with marker points, which serve as reference zero points for each movement of the driving mechanism during continuous experiments.

Images