An optical detection device based on a microfluidic chip

Abstract

The utility model discloses a kind of multi-channel optical detection device based on microfluidic chip, it includes sequentially arranged excitation light component, excitation light path component, dichroic mirror component, mirror component and camera lens component along excitation light path direction;Excitation light component is used for excitation light emission in detection process;Excitation light path component is used for excitation light path model control in detection process, dichroic mirror component is used for excitation light and receiving light separation control in detection process, mirror component is used for receiving light path control in detection process, camera lens component is used for receiving light signal in detection process and carries out signal acquisition.The utility model uses high-definition camera shooting technology and microfluidic chip technology, by specific light path component, circular excitation light beam is transformed into linear beam, improves detection light uniformity and stretches optical detection area, simultaneously detects multiple point positions on microfluidic chip, obtains multiple detection results in turn, can effectively improve detection efficiency.

Description

Technical Field

[0001] This utility model belongs to the field of nucleic acid detection instrument and POCT fluorescence detection technology, specifically relating to a multi-channel optical detection device based on a microfluidic chip. Background Technology

[0002] Microfluidics refers to systems that use microchannels to process or manipulate tiny fluids, and it is a detection technology used in biology and biomedical engineering. Because of their miniaturization and integration characteristics, microfluidic devices are often called microfluidic chips, and are also known as lab-on-a-chip systems and micrototal analysis systems.

[0003] PCR (polymerase chain reaction) is a molecular biology technique for detecting, amplifying, and expanding specific nucleic acid fragment sequences in vitro. Due to its high specificity, high sensitivity, and rapid detection, it is widely used in molecular biology detection and analysis.

[0004] Currently, most POCT microfluidic chip detectors that combine microfluidics and fluorescent labeling of PCR amplification products use a circular spot illumination method and perform sequential detection, which results in relatively low detection efficiency. Utility Model Content

[0005] The purpose of this invention is to provide a multi-channel optical detection device based on a microfluidic chip. This device uses high-definition camera imaging technology and microfluidic chip technology. By setting optical path components, it transforms a circular excitation beam into a linear beam, improves the uniformity of the detection light and stretches the detection area of ​​the light. At the same time, it detects multiple sets of points on the microfluidic chip and obtains multiple detection results in sequence, which can effectively improve the detection efficiency.

[0006] To achieve the above objectives, this utility model adopts the following technical solution: a multi-channel optical detection device based on a microfluidic chip, which includes an excitation light assembly, an excitation light path assembly, a dichroic mirror assembly, a reflector assembly, and a camera lens assembly arranged sequentially along the excitation light path direction;

[0007] The excitation light component is used to detect the emission of excitation light during the detection process. The excitation light component mainly consists of an LED heat sink and an LED lamp board fixed on the LED heat sink. The excitation light component is installed on one side of the excitation light path component.

[0008] The excitation optical path assembly is used for controlling the excitation optical path model during the detection process. The excitation optical path assembly mainly consists of a lens mount, a plano-convex lens I, an excitation light filter, a plano-convex lens II, a cylindrical lens mount, a plano-concave lens, a cylindrical lens, and a mounting plate. The plano-convex lens I, the excitation light filter, and the plano-convex lens II are fixed sequentially on the lens mount along the excitation optical path direction to purify the point light source. The plano-concave lens and the cylindrical lens are fixed sequentially on the cylindrical lens mount along the excitation optical path direction to convert the point light source into a linear light source. The mounting plate is fixed on both sides of the cylindrical lens mount.

[0009] The dichroic mirror assembly is used for the separation and control of excitation light and receiving light during the detection process. The dichroic mirror assembly mainly consists of a large reflecting mirror base, a dichroic mirror, and a fluorescent filter. The dichroic mirror and the fluorescent filter are installed on the large reflecting mirror base. The dichroic mirror is used to separate the light path of the excitation light reflection and the light path of the receiving light transmission. The fluorescent filter is used to purify the receiving light to reduce stray light interference. A sample holding tray is provided below the large reflecting mirror base. The dichroic mirror is set at a 45° angle on the large reflecting mirror base. It is used to convert the horizontally incident excitation light into a linear light source, which is reflected at a 90° angle and emitted vertically downwards onto multiple sets of sample objects placed side by side below the dichroic mirror. The fluorescent filter is set horizontally on the large reflecting mirror base above the dichroic mirror. The dichroic mirror assembly is installed on the excitation light path assembly.

[0010] The reflector assembly is used to control the optical path model of the received light during the detection process. The reflector assembly mainly consists of a small reflector mount and a reflector. The reflector is installed on the small reflector mount at a 45° angle. The reflector is set parallel to the dichroic mirror and is used to reflect the light source that passes vertically upward through the dichroic mirror into a horizontal direction by 90° and emit it to the camera lens assembly. The reflector assembly is fixedly connected to the excitation optical path assembly and the dichroic mirror assembly respectively.

[0011] The camera lens assembly is used to receive light signals and acquire signals during the detection process. The camera lens assembly mainly consists of a camera, a lens and a camera mounting base. The camera and lens are connected through corresponding interfaces. The lens is set horizontally and the lens incident end is opposite to the reflection direction of the light source of the reflector. It is used to receive light signals reflected by the emitting mirror. The camera is fixed on the camera mounting base and the camera lens assembly is fixed on the upper part of the excitation light path assembly.

[0012] Furthermore, the LED light board is fixed to one side of the LED heat sink with screws. The LED heat sink has a light path through hole in the middle that is opposite to the light-emitting side of the LED light board. The other side of the LED heat sink is fixed to the right side wall of the cylindrical mirror seat in the excitation light assembly with screws. The excitation light of the LED light board passes sequentially through the planar convex lens I, the excitation light filter and the planar convex lens II on the lens seat, as well as the plano-concave lens and the cylindrical mirror on the cylindrical mirror seat.

[0013] Furthermore, the excitation light incident end of the lens mount is installed in the optical path through hole in the middle of the LED heat sink, and the excitation light emitting end of the lens mount is installed on the cylindrical lens mount. The mounting plate is fixed to the front and rear side walls of the cylindrical lens mount by screws. The upper part of the mounting plate is used to install the camera lens assembly. The left side of the mounting plate has a 45° inclined structure and is used to install the upper and lower reflector assembly and dichroic mirror assembly. The bottom of the mounting plate is fitted with a tray for placing multiple sets of test samples side by side by screws.

[0014] Furthermore, the large reflector mount is a right-angled triangular prism frame structure, the dichroic mirror is inclined at 45° on the inclined side of the right-angled triangular prism frame, the fluorescent filter is horizontally arranged on the right-angled side of the right-angled triangular prism frame, and the inclined side of the right-angled triangular prism frame is arranged on the 45° inclined structure of the mounting plate.

[0015] Furthermore, the small reflector mount is a right-angled triangular prism frame structure, and the emitting mirror is inclined at 45° on the inclined side of the right-angled triangular prism frame. The small reflector mount is fixed to the large reflector mount and the mounting plate by screws.

[0016] Furthermore, the camera mounting base is located on both sides of the camera, and the bottom of the camera mounting base is fixed to the upper part of the cylindrical lens mount by screws.

[0017] Compared with the prior art, this utility model has the following advantages and beneficial effects: This utility model is small in size, occupies little space inside the instrument, and is easy to install; This utility model has a high degree of modular integration, and can be matched with different optical path lens combinations for fluorescence detection according to the actual needs of fluorescent dyes, which is convenient, fast and highly practical; This utility model improves the resolution based on a high-definition camera, and can simultaneously detect multiple points on the microfluidic chip. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the overall structure of this utility model.

[0019] Figure 2 This is a schematic diagram of the assembly structure of this utility model.

[0020] Figure 3 This is a schematic diagram of the structure of the excitation light component in this utility model.

[0021] Figure 4 This is a schematic diagram of the excitation optical path component in this utility model.

[0022] Figure 5 This is a schematic diagram of the structure of the dichroic mirror assembly in this utility model.

[0023] Figure 6 This is a schematic diagram of the reflector assembly in this utility model.

[0024] Figure 7 This is a schematic diagram of the camera lens assembly in this utility model.

[0025] In the diagram: 100 - excitation light assembly, 101 - LED heat sink, 102 - LED light board;

[0026] 200-Excitation optical path assembly, 201-Lens mount, 202-Cylindrical lens mount, 203-Mounting plate, 204-Planto-convex lens I, 205-Excitation light filter, 206-Planto-convex lens II, 207-Planto-concave lens, 208-Cylindrical lens;

[0027] 300-Dichromatic mirror assembly, 301-Large emission mirror mount, 302-Dichromatic mirror, 303-Fluorescent filter;

[0028] 400 - Reflector assembly, 401 - Small reflector mount, 402 - Reflector;

[0029] 500 - Camera lens assembly; 501 - Camera; 502 - Lens; 503 - Camera mount. Detailed Implementation

[0030] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0031] A multi-channel optical detection device based on a microfluidic chip includes an excitation light assembly 100, an excitation light path group 200, a dichroic mirror assembly 300, a reflector assembly 400, and a camera lens assembly 500 arranged sequentially along the excitation light path direction.

[0032] The excitation light assembly 100 is used to detect the emission of excitation light during the detection process. The excitation light assembly 100 mainly consists of an LED heat sink 101 and an LED lamp board 102 fixed on the LED heat sink 101. The excitation light assembly 100 is installed on one side of the excitation light path assembly 200.

[0033] The excitation optical path assembly 200 is used for controlling the excitation optical path model during the detection process. The excitation optical path assembly 200 mainly consists of a lens holder 201, a plano-convex lens I 204, an excitation light filter 205, a plano-convex lens II 206, a cylindrical lens holder 202, a plano-concave lens 207, a cylindrical lens 208, and a mounting plate 203. The plano-convex lens I 204, the excitation light filter 205, and the plano-convex lens II 206 are sequentially fixed on the lens holder 201 along the excitation optical path direction to achieve purification of the point light source. The plano-concave lens 207 and the cylindrical lens 208 are sequentially fixed on the cylindrical lens holder 202 along the excitation optical path direction to achieve conversion of the point light source into a linear light source. The mounting plate 203 is fixed on both sides of the cylindrical lens holder 202.

[0034] The dichroic mirror assembly 300 is used for the separation and control of excitation light and received light during the detection process. The dichroic mirror assembly 300 mainly consists of a large reflecting mirror mount 301, a dichroic mirror 302, and a fluorescence filter 303. The dichroic mirror 302 and the fluorescence filter 303 are mounted on the large reflecting mirror mount 301. The dichroic mirror 302 separates the light paths of the excitation light reflection and the received light transmission, while the fluorescence filter 303 purifies the received light to reduce stray light interference. The large reflecting mirror... Below the base 301 is a sample holding tray. The dichroic mirror 302 is tilted at 45° on the large reflector base 301. It is used to convert the horizontally incident excitation light into a linear light source, which is reflected at 90° and emitted vertically downwards onto multiple sets of samples placed side by side below the dichroic mirror 302. The fluorescent filter 303 is horizontally set on the large reflector base 301 above the dichroic mirror 302. The dichroic mirror assembly 300 is mounted on the excitation optical path assembly 200.

[0035] The reflector assembly 400 is used for controlling the optical path model of the received light during the detection process. The reflector assembly 400 mainly consists of a small reflector mount 401 and a reflector 402. The reflector 402 is installed on the small reflector mount 401 at a 45° angle. The reflector 402 is arranged parallel to and opposite to the dichroic mirror 302. It is used to reflect the light source that passes vertically upward through the dichroic mirror 302 into a horizontal direction by 90° and emit it to the camera lens assembly 500. The reflector assembly 400 is fixedly connected to the excitation optical path assembly 200 and the dichroic mirror assembly 300 respectively.

[0036] The camera lens assembly 500 is used to receive light signals and acquire signals during the detection process. The camera lens assembly 500 mainly consists of a camera 501, a lens 502 and a camera mounting base 503. The camera 501 and the lens 502 are connected through corresponding interfaces. The lens 502 is horizontally set and the incident end of the lens 502 is opposite to the light source reflection direction of the reflector 402. It is used to receive light signals reflected by the emitting mirror 402. The camera 501 is fixed on the camera mounting base 503 and the camera lens assembly 500 is fixed on the upper part of the excitation light path assembly 200.

[0037] The LED lamp board 102 of this utility model is fixed to one side of the LED heat sink 101 by screws. The LED heat sink 101 has a light path through hole in the middle that is opposite to the light-emitting side of the LED lamp board 102. The other side of the LED heat sink 101 is fixed to the right side wall of the cylindrical lens seat 202 in the excitation light assembly 200 by screws. The excitation light of the LED lamp board 102 passes through the planar convex lens I 204, the excitation light filter 205 and the planar convex lens II 206 on the lens seat 201, as well as the plano-concave lens 207 and the cylindrical lens 208 on the cylindrical lens seat 202 in sequence.

[0038] The excitation light incident end of the lens mount 201 described in this utility model is installed in the light path through hole in the middle of the LED heat sink 101, and the excitation light emitting end of the lens mount 201 is installed on the cylindrical lens mount 202. The mounting plate 203 is fixed to the front and rear side walls of the cylindrical lens mount 202 by screws. The upper part of the mounting plate 203 is used to install the camera lens assembly 500. The left side of the mounting plate 203 has a 45° inclined structure and is used to install the upper and lower reflector assembly 400 and the dichroic mirror assembly 300. The bottom of the mounting plate 203 is fitted with a tray for placing multiple sets of test samples side by side by screws.

[0039] The large reflector mount 301 of this utility model is a right-angled triangular prism frame structure. The dichroic mirror 302 is inclined at 45° on the inclined side of the right-angled triangular prism frame. The fluorescent filter 303 is horizontally arranged on the right-angled side of the right-angled triangular prism frame. The inclined side of the right-angled triangular prism frame is arranged on the 45° inclined structure of the mounting plate 203.

[0040] The small reflector base 401 of this utility model is a right-angled triangular prism frame structure. The reflector 402 is inclined at 45° on the inclined side of the right-angled triangular prism frame. The small reflector base 401 is fixed to the large reflector base 301 and the mounting plate 203 by screws.

[0041] The camera mounting base 503 of this utility model is disposed on both sides of the camera 501, and the bottom of the camera mounting base 503 is fixed to the upper part of the cylindrical lens base 202 by screws.

[0042] The usage process of this utility model is as follows: First, multiple sets of test samples are placed side by side on the tray below the dichroic mirror. The LED light panel is turned on, and the excitation light is purified by passing through a plano-convex lens I, an excitation light filter, and a plano-convex lens II in sequence. Then, the point light source is converted into a linear light source by passing through a plano-concave lens and a cylindrical lens in sequence. Part of the linear light source is reflected by the dichroic mirror and emitted to the multiple sets of test samples placed side by side. After being reflected by the samples, it is transmitted through the dichroic mirror and emitted to the reflector. The other part of the linear light source is directly transmitted through the dichroic mirror and emitted to the reflector. The camera lens assembly is used to receive light signals and collect signals during the detection process, and transmit the collected signals to the test system for detection analysis and data generation.

[0043] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention.

Claims

1. A multi-channel optical detection device based on a microfluidic chip, characterized in that... It includes an excitation light assembly, an excitation light path assembly, a dichroic mirror assembly, a reflector assembly, and a camera lens assembly arranged sequentially along the excitation light path direction; The excitation light component is used to detect the emission of excitation light during the detection process. The excitation light component mainly consists of an LED heat sink and an LED lamp board fixed on the LED heat sink. The excitation light component is installed on one side of the excitation light path component. The excitation optical path assembly is used for controlling the excitation optical path model during the detection process. The excitation optical path assembly mainly consists of a lens mount, a plano-convex lens I, an excitation light filter, a plano-convex lens II, a cylindrical lens mount, a plano-concave lens, a cylindrical lens, and a mounting plate. The plano-convex lens I, the excitation light filter, and the plano-convex lens II are fixed sequentially on the lens mount along the excitation optical path direction to purify the point light source. The plano-concave lens and the cylindrical lens are fixed sequentially on the cylindrical lens mount along the excitation optical path direction to convert the point light source into a linear light source. The mounting plate is fixed on both sides of the cylindrical lens mount. The dichroic mirror assembly is used for the separation and control of excitation light and receiving light during the detection process. The dichroic mirror assembly mainly consists of a large reflecting mirror base, a dichroic mirror, and a fluorescent filter. The dichroic mirror and the fluorescent filter are installed on the large reflecting mirror base. The dichroic mirror is used to separate the light path of the excitation light reflection and the light path of the receiving light transmission. The fluorescent filter is used to purify the receiving light to reduce stray light interference. A sample holding tray is provided below the large reflecting mirror base. The dichroic mirror is set at a 45° angle on the large reflecting mirror base. It is used to convert the horizontally incident excitation light into a linear light source, which is reflected at a 90° angle and emitted vertically downwards onto multiple sets of sample objects placed side by side below the dichroic mirror. The fluorescent filter is set horizontally on the large reflecting mirror base above the dichroic mirror. The dichroic mirror assembly is installed on the excitation light path assembly. The reflector assembly is used to control the optical path model of the received light during the detection process. The reflector assembly mainly consists of a small reflector mount and a reflector. The reflector is installed on the small reflector mount at a 45° angle. The reflector is set parallel to the dichroic mirror and is used to reflect the light source that passes vertically upward through the dichroic mirror into a horizontal direction by 90° and emit it to the camera lens assembly. The reflector assembly is fixedly connected to the excitation optical path assembly and the dichroic mirror assembly respectively. The camera lens assembly is used to receive light signals and acquire signals during the detection process. The camera lens assembly mainly consists of a camera, a lens and a camera mounting base. The camera and lens are connected through corresponding interfaces. The lens is set horizontally and the lens incident end is opposite to the reflection direction of the light source of the reflector. It is used to receive light signals reflected by the emitting mirror. The camera is fixed on the camera mounting base and the camera lens assembly is fixed on the upper part of the excitation light path assembly.

2. The multi-channel optical detection device based on a microfluidic chip according to claim 1, characterized in that: The LED light board is fixed to one side of the LED heat sink with screws. The LED heat sink has a light path through hole in the middle that is opposite to the light-emitting side of the LED light board. The other side of the LED heat sink is fixed to the right side wall of the cylindrical mirror seat in the excitation light assembly with screws. The excitation light of the LED light board passes through the planar convex lens I, the excitation light filter and the planar convex lens II on the lens seat, as well as the plano-concave lens and the cylindrical mirror on the cylindrical mirror seat in sequence.

3. The multi-channel optical detection device based on a microfluidic chip according to claim 1, characterized in that: The excitation light incident end of the lens mount is installed in the optical path through hole in the middle of the LED heat sink, and the excitation light emitting end of the lens mount is installed on the cylindrical lens mount. The mounting plate is fixed to the front and rear side walls of the cylindrical lens mount by screws. The upper part of the mounting plate is used to install the camera lens assembly. The left side of the mounting plate has a 45° inclined structure and is used to install the upper and lower reflector assembly and dichroic mirror assembly. The bottom of the mounting plate is fitted with a tray for placing multiple sets of test samples side by side by screws.

4. The multi-channel optical detection device based on a microfluidic chip according to claim 1, characterized in that: The large reflector mount is a right-angled triangular prism frame structure. The dichroic mirror is inclined at 45° on the inclined side of the right-angled triangular prism frame, and the fluorescent filter is horizontally set on the right-angled side of the right-angled triangular prism frame. The inclined side of the right-angled triangular prism frame is set on the 45° inclined structure of the mounting plate.

5. A multi-channel optical detection device based on a microfluidic chip according to claim 1, characterized in that: The small reflector mount is a right-angled triangular prism frame structure. The reflector is inclined at 45° on the inclined side of the right-angled triangular prism frame. The small reflector mount is fixed to the large reflector mount and the mounting plate by screws.

6. The multi-channel optical detection device based on a microfluidic chip according to claim 1, characterized in that: The camera mounting base is located on both sides of the camera, and the bottom of the camera mounting base is fixed to the upper part of the cylindrical lens mount with screws.

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