A chromatography packing device for bovine serum purification

Abstract

This utility model relates to the field of chromatographic packing, and discloses a chromatographic packing device for bovine serum purification, including a base, a column fixedly connected to the upper side of the base, a fixing block fixedly connected to the outer wall of the column, a fixing clamping block fixedly connected to the right side of the fixing block, an anti-slip strip fixedly connected to the inner side of the fixing clamping block, and a sliding column slidably connected to the inner wall of the fixing block. In this utility model, by placing the chromatographic column tube on the anti-slip strip between the fixing clamping block and the moving clamping block, the moving clamping block moves towards the fixing clamping block, and the upper side of the moving clamping block presses against the inclined surface of the locking block, causing the locking block to slide upward against the elastic force of the first spring; when the moving clamping block is adjusted to a position in close contact with the chromatographic column tube, the locking block moves downward under the elastic reset action of the first spring, and its lower side engages with the upper inner wall of the moving clamping block, facilitating quick and easy limiting and locking of the moving clamping block.

Description

Technical Field

[0001] This utility model relates to the field of chromatographic packing, and more particularly to a chromatographic packing device for bovine serum purification. Background Technology

[0002] Bovine serum purification is a key technology in fields such as biopharmaceuticals and cell culture. The core of it is to remove blood cells, fibrin, impurity proteins and contaminants (such as viruses and mycoplasma) from bovine blood to obtain high-purity, low-risk serum components (such as fetal bovine serum FBS and newborn calf serum NBS) to meet the stringent requirements of cell growth and biopharmaceutical production.

[0003] A search revealed Chinese Patent Publication No. CN220877820U, which discloses a rapid packing device for chromatography column packing. The device includes a fixed base plate, a chromatography column tube, and a connecting outlet pipe. A fixed upright is mounted on the top of the fixed base plate, and a chromatography column fixing mechanism is mounted on the outer side of the fixed upright. A loading cylinder is connected to the top of the fixed upright via a bracket. A packing tube is connected to the outer side of the loading cylinder, and a stirring motor is mounted on the top of the loading cylinder. The drive end of the stirring motor passes through the top of the loading cylinder and is connected to a stirring rod. A stirring blade is connected to the bottom of the stirring rod. An outlet pipe is connected to the bottom of the loading cylinder, and a drip pump is installed in the middle section of the outlet pipe. A water outlet nozzle is connected to the bottom of the drip pump, and an annular bracket is fitted around the outer side of the water outlet nozzle. A flow guide support is fixed to the bottom of the annular bracket. This invention, by providing a chromatography column fixing mechanism on the fixed upright for fixing the chromatography column tube, eliminates the need for removing the bracket separately, saving operation time.

[0004] In the above-mentioned technologies, although the chromatography column tube is fixed by a fixing mechanism, it is inconvenient to fix the chromatography column tube by fixing mechanisms such as the first locking bolt and the second locking bolt. Therefore, a chromatography packing device for bovine serum purification is proposed to solve the above problems. Utility Model Content

[0005] To overcome the above shortcomings, this utility model provides a chromatography packing device for bovine serum purification, which aims to improve the problem of inconvenient fixation in the prior art.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: a chromatography packing device for bovine serum purification, comprising a base, a column fixedly connected to the upper side of the base, a fixing block fixedly connected to the outer wall of the column, a fixing clamping block fixedly connected to the right side of the fixing block, an anti-slip strip fixedly connected to the inner side of the fixing clamping block, a sliding column slidably connected to the inner wall of the fixing block, a movable clamping block fixedly connected to the front side of the sliding column, a limiting component provided on the upper side of the fixing block for limiting the movable clamping block, a chromatography column tube clamped on the anti-slip strip, an outlet pipe fixedly connected to the lower side of the chromatography column tube, and a packing assembly provided on the upper side of the column for packing.

[0007] As a further description of the above technical solution:

[0008] The packing assembly includes a liquid tank, which is fixedly connected to the upper side of the column. A water outlet is fixedly connected to the lower side of the liquid tank, and a drip pump is fixedly connected to the water outlet. A fixing base is fixedly connected to the upper side of the liquid tank. A retaining post is elastically connected to the right inner wall of the fixing base via two springs. A bucket lid is snapped onto the outer wall of the fixing base. A motor is fixedly connected to the upper side of the bucket lid. A stirring rod is rotatably connected through the inner wall of the bucket lid. The upper inner wall of the stirring rod is fixedly connected to the output shaft of the motor.

[0009] As a further description of the above technical solution:

[0010] The limiting component includes a locking block, which is elastically connected to the upper inner wall of the fixed block by a spring.

[0011] As a further description of the above technical solution:

[0012] One end of the spring is fixedly connected to the upper inner wall of the fixing block, and the other end of the spring is fixedly connected to the outer wall of the card block.

[0013] As a further description of the above technical solution:

[0014] The lower side of the card block is inclined, the outer wall of the card block is slidably connected to the inner wall of the fixed block, and the lower side of the card block is engaged with the upper inner wall of the movable clamping block.

[0015] As a further description of the above technical solution:

[0016] One end of the second spring is fixedly connected to the inner wall of the right side of the fixed base, and the other end of the second spring is fixedly connected to the left side of the locking post.

[0017] As a further description of the above technical solution:

[0018] The outer wall of the locking pin is slidably connected to the inner wall of the right side of the fixing seat, and the outer wall of the locking pin is engaged with the inner wall of the bucket lid.

[0019] As a further description of the above technical solution:

[0020] A feed pipe is provided on the upper side of the barrel lid.

[0021] This utility model has the following beneficial effects:

[0022] 1. In this utility model, the chromatography column is placed on the anti-slip strip between the fixed clamp and the movable clamp. The movable clamp moves towards the fixed clamp, and the upper side of the movable clamp presses against the inclined surface of the locking block, causing the locking block to slide upward against the elastic force of the first spring. When the movable clamp is adjusted to a position in close contact with the chromatography column, the locking block moves downward under the elastic reset action of the first spring, and its lower side is locked into the upper inner wall of the movable clamp, which facilitates quick and easy limiting and locking of the movable clamp.

[0023] 2. In this utility model, the motor inside the liquid container drives the stirring rod to rotate, which can continuously stir the bovine serum chromatography packing material and prevent the packing material from settling; at the same time, the drip pump can precisely control the dripping speed of the packing material from the water outlet, so that the packing material in the chromatography column is filled more evenly, which ensures the effect of subsequent bovine serum purification chromatography operations. Attached Figure Description

[0024] Figure 1 This is a three-dimensional schematic diagram of a chromatography packing device for purifying bovine serum proposed in this utility model;

[0025] Figure 2 This is a schematic diagram of the moving clamp and the fixed clamp of a chromatography packing device for bovine serum purification proposed in this utility model;

[0026] Figure 3 This is a schematic diagram of the moving clamp and locking block of a chromatography packing device for bovine serum purification proposed in this utility model;

[0027] Figure 4 This is a cross-sectional schematic diagram of the barrel lid of a chromatography packing device for bovine serum purification proposed in this utility model;

[0028] Figure 5 This utility model proposes a chromatography packing device for purifying bovine serum. Figure 4 Enlarged diagram of point A.

[0029] Legend:

[0030] 1. Base; 2. Liquid outlet pipe; 3. Chromatography column tube; 4. Water outlet; 5. Drip pump; 6. Liquid container; 7. Container lid; 8. Motor; 9. Column; 10. Fixing block; 11. Fixing clamp; 12. Anti-slip strip; 13. Moving clamp; 14. Locking block; 15. Spring 1; 16. Sliding column; 17. Stirring rod; 18. Spring 2; 19. Locking column; 20. Fixing base. Detailed Implementation

[0031] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0032] Reference Figures 1-3 This utility model provides an embodiment of a chromatography packing device for bovine serum purification, comprising a base 1, which provides support for the packing device. A column 9 is fixedly connected to the upper side of the base 1, providing support for the fixing block 10. A fixing block 10 is fixedly connected to the outer wall of the column 9. A fixing clamp 11 is fixedly connected to the right side of the fixing block 10. An anti-slip strip 12 is fixedly connected to the inner side of the fixing clamp 11 to increase the friction between the clamp and the chromatography column 3, thereby improving the efficiency of the packing device. For clamping stability, a sliding column 16 is slidably connected to the inner wall of the fixed block 10, and a movable clamping block 13 is fixedly connected to the front side of the sliding column 16. A limiting component is provided on the upper side of the fixed block 10 to limit the movable clamping block 13. The anti-slip strip 12 clamps the chromatography column tube 3, which is the core component of the bovine serum purification chromatography operation. An outlet tube 2 is fixedly connected to the lower side of the chromatography column tube 3 to export and collect the chromatographic liquid. A packing assembly is provided on the upper side of the column 9 for packing.

[0033] Reference Figure 1 , Figure 4 and Figure 5The packing assembly includes a liquid tank 6 for storing bovine serum chromatography packing material to be packed. The liquid tank 6 is fixedly connected to the upper side of the column 9. A water outlet 4 is fixedly connected to the lower side of the liquid tank 6, forming a channel structure for the packing material to flow from the liquid tank 6 to the chromatography column tube 3. A drip pump 5 is fixedly connected to the water outlet 4, which can precisely control the dripping speed of the packing material and ensure the uniformity of the packing process. A fixing seat 20 is fixedly connected to the upper side of the liquid tank 6. A retaining post 19 is elastically connected to the inner right side of the fixing seat 20 via a spring 18. The spring 18 can drive the retaining post 19 to automatically reset, realizing the retaining function. The outer wall of the retaining post 19 is slidably connected to the inner right side of the fixing seat 20, and the outer wall of the retaining post 19 is engaged with the column tube 3. The inner wall of the lid 7 is used to quickly and easily clamp the lid 7 for easy disassembly and maintenance. One end of the second spring 18 is fixedly connected to the right inner wall of the fixing base 20, and the other end of the second spring 18 is fixedly connected to the left side of the locking post 19. The lid 7 is clamped onto the outer wall of the fixing base 20. A feed pipe is provided on the upper side of the lid 7 for adding chromatography packing material into the liquid container 6. A motor 8 is fixedly connected to the upper side of the lid 7 to provide power for the rotation of the stirring rod 17. The stirring rod 17 is rotatably connected through the inner wall of the lid 7 to stir and mix the chromatography packing material in the liquid container 6 to prevent packing material from settling. The upper inner wall of the stirring rod 17 is fixedly connected to the output shaft of the motor 8.

[0034] Reference Figure 2 and Figure 3 The limiting component includes a locking block 14, the lower side of which is inclined. The outer wall of the locking block 14 is slidably connected to the inner wall of the fixed block 10. The lower side of the locking block 14 is engaged with the upper inner wall of the movable clamping block 13 to limit and lock the position of the movable clamping block 13, ensuring that the chromatography column tube 3 is stably clamped. The locking block 14 is elastically connected to the upper inner wall of the fixed block 10 by a spring 15. The spring 15 provides downward elastic pressure to the locking block 14 to improve the stability of the engagement. One end of the spring 15 is fixedly connected to the upper inner wall of the fixed block 10, and the other end of the spring 15 is fixedly connected to the outer wall of the locking block 14.

[0035] Working principle: The chromatography column 3 is placed on the anti-slip strip 12 between the fixed clamp 11 and the movable clamp 13. The movable clamp 13 moves towards the fixed clamp 11. During the movement, the upper side of the movable clamp 13 presses against the inclined surface of the locking block 14, causing the locking block 14 to slide upward against the elastic force of the spring 15. When the movable clamp 13 is adjusted to a position in close contact with the chromatography column 3, the locking block 14 moves downward under the elastic reset action of the spring 15, and its lower side engages with the upper inner wall of the movable clamp 13, completing the limiting and locking of the movable clamp 13. At this time, the anti-slip strip 12 further ensures that the chromatography column 3 is stably clamped by increasing the friction.

[0036] Bovine serum chromatography packing material is added to the liquid container 6 through the feed pipe on the lid 7. When the lid 7 is closed, the locking pin 19 in the fixing seat 20 is engaged with the inner wall of the lid 7 by the elastic force of the spring 18, thus quickly fixing the lid 7. The motor 8 is started, and its output shaft drives the stirring rod 17 to rotate inside the liquid container 6, continuously stirring the packing material and preventing it from settling.

[0037] Finally, the drip pump 5 is started, and the chromatography packing material in the liquid filling tank 6 is precisely controlled to drip evenly into the fixed chromatography column tube 3 below through the water outlet 4, completing the packing operation; during or after the filling process, the liquid in the chromatography column tube 3 can be discharged and collected through the liquid outlet tube 2 on the lower side. The entire process achieves stable and uniform filling of the chromatography packing material through the coordinated cooperation of various components.

[0038] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A chromatography packing device for purifying bovine serum, comprising a base (1), characterized in that: A column (9) is fixedly connected to the upper side of the base (1). A fixing block (10) is fixedly connected to the outer wall of the column (9). A fixing clamp (11) is fixedly connected to the right side of the fixing block (10). An anti-slip strip (12) is fixedly connected to the inner side of the fixing clamp (11). A sliding column (16) is slidably connected to the inner wall of the fixing block (10). A movable clamp (13) is fixedly connected to the front side of the sliding column (16). A limiting component is provided on the upper side of the fixing block (10). The limiting component is used to limit the movable clamp (13). A chromatography column tube (3) is clamped on the anti-slip strip (12). An outlet pipe (2) is fixedly connected to the lower side of the chromatography column tube (3). A packing assembly is provided on the upper side of the column (9). The packing assembly is used for packing.

2. The chromatographic packing device for bovine serum purification according to claim 1, characterized in that: The packing assembly includes a liquid tank (6), which is fixedly connected to the upper side of the column (9). A water outlet (4) is fixedly connected to the lower side of the liquid tank (6). A drip pump (5) is fixedly connected to the water outlet (4). A fixed base (20) is fixedly connected to the upper side of the liquid tank (6). A locking post (19) is elastically connected to the inner right side of the fixed base (20) via a spring (18). A bucket lid (7) is snapped onto the outer wall of the fixed base (20). A motor (8) is fixedly connected to the upper side of the bucket lid (7). A stirring rod (17) is rotatably connected through the inner wall of the bucket lid (7). The upper inner wall of the stirring rod (17) is fixedly connected to the output shaft of the motor (8).

3. The chromatographic packing device for bovine serum purification according to claim 1, characterized in that: The limiting component includes a locking block (14), which is elastically connected to the upper inner wall of the fixing block (10) by a spring (15).

4. The chromatographic packing device for bovine serum purification according to claim 3, characterized in that: One end of the spring (15) is fixedly connected to the upper inner wall of the fixing block (10), and the other end of the spring (15) is fixedly connected to the outer wall of the card block (14).

5. The chromatographic packing device for bovine serum purification according to claim 3, characterized in that: The lower side of the card block (14) is inclined, the outer wall of the card block (14) is slidably connected to the inner wall of the fixed block (10), and the lower side of the card block (14) is engaged with the upper inner wall of the movable clamping block (13).

6. The chromatographic packing device for bovine serum purification according to claim 2, characterized in that: One end of the second spring (18) is fixedly connected to the inner right side of the fixed base (20), and the other end of the second spring (18) is fixedly connected to the left side of the locking post (19).

7. The chromatographic packing device for bovine serum purification according to claim 2, characterized in that: The outer wall of the locking post (19) is slidably connected to the inner right wall of the fixing seat (20), and the outer wall of the locking post (19) is engaged with the inner wall of the bucket lid (7).

8. The chromatographic packing device for bovine serum purification according to claim 2, characterized in that: A feed pipe is provided on the upper side of the barrel lid (7).

Images