An electrochemical biosensor for use in the determination of glyphosate and the preparation method thereof

EP4677352A4Pending Publication Date: 2026-06-24BILECIK SEYH EDEBALI UNIVERSITESI +1
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Patent Information

Application Number
EP2024767526
Authority / Receiving Office
EP · EP
Patent Type
Applications
Current Assignee / Owner
Filing Date
2024-03-11
Publication Date
2026-06-24

AI Technical Summary

Technical Problem

Current electrochemical biosensors for glyphosate detection are not sensitive, reliable, eco-friendly, user-friendly, or cost-effective, requiring complex preparation procedures, expensive metal electrodes, and are not suitable for miniaturization or disposable chip technology.

Method used

A disposable electrochemical biosensor using a carboxymethyl cellulose/hydroxyapatite nanobiocomposite modified pencil graphite electrode with a DNA aptamer in a G-quadruplex structure, which is specifically immobilized on the surface for sensitive glyphosate detection, allowing for low-cost, eco-friendly, and practical analysis.

Benefits of technology

The biosensor achieves high accuracy and reliability in glyphosate detection with a low-cost, environmentally friendly, and user-friendly approach, suitable for miniaturization and disposable chip technology, with a detection limit of 0.04 pg/mL and sensitivity of 0.57 pA.mL/pg.cm².

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Abstract

The invention relates to a disposable electrochemical biosensor for use in the sensitive determination of glyphosate (GLY), a herbicide, and the preparation method thereof. In the electrochemical biosensor of the invention, carboxymethyl cellulose (CMC) / hydroxyapatite (HaNP) nanobiocomposite was modified onto the surface of a disposable pencil graphite electrode (PGE). DNA aptamer (DNA APT), a specific bioreceptor of glyphosate, was immobilised on the CMC / HaNP-PGE surface.
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