Method for reduction of host cell proteins in affinity chromatography

Abstract

To provide a method for purifying a protein for obtaining an antibody with reduced content of impurities (e.g., a host cell protein) in affinity chromatography.SOLUTION: Disclosed herein is a method for purifying an antibody by reducing the content of a host cell protein; in particular, a method for using a low conductivity aqueous solution in a wash step of protein A chromatography for reducing the content of a host cell protein, where the protein A chromatography is used to purify a human IgG1 or IgG4 isotype antibody, and where the low conductivity aqueous solution has a conductivity value of about 0.5 mS / cm or less.SELECTED DRAWING: None

Description

[Technical field]

[0001] The present invention relates to the field of polypeptide purification. The present invention is particularly concerned with the reduction of host cell proteins such as phospholipase B-like 2 (PLBL2) or clusterin in solutions containing antibodies. [Background technology]

[0002] 2. Background of the Invention Proteins, especially immunoglobulins, play an important role in the modern medical portfolio. To be applied in humans, every therapeutic protein must meet clear criteria. To ensure the safety of biologics for humans, it is necessary, among other things, to remove by-products that accumulate during the production process. To meet regulatory specifications, one or more purification steps must be performed after the manufacturing process. Purity, throughput, and yield, among others, play a key role in determining the appropriate purification process.

[0003] A variety of methods are established and widely used for the purification of proteins, such as affinity chromatography (e.g., protein A or protein G affinity chromatography, single-chain Fv ligand affinity chromatography), ion-exchange chromatography (e.g., cation exchange (sulfopropyl or carboxymethyl resins), anion exchange (aminoethyl resins) and mixed-mode ion exchange), thiophilic adsorption (e.g., with beta-mercaptoethanol ligands and other SH ligands), hydrophobic interaction or aromatic adsorption chromatography (e.g., with phenyl-sepharose, aza-arenophilic resins, or m-aminophenylboronic acid), metal chelate affinity chromatography (e.g., with Ni(II)-affinity and Cu(II)-affinity materials), size-exclusion chromatography, and electrophoretic methods (gel electrophoresis, capillary electrophoresis, etc.).

[0004] The purification of recombinantly produced immunoglobulins often uses a combination of different column chromatography steps. During purification, host cell proteins and host cell DNA as well as non-immunoglobulin contaminants such as endotoxins and viruses are depleted. Therefore, an affinity chromatography step such as Protein A affinity chromatography is generally followed by one or more additional separation steps. In general, the washing steps of affinity chromatography methods are described as using high conductivity buffers.

[0005] US 6,127,526 (Patent Document 1) describes a method for purifying a protein by Protein A chromatography, which includes the steps of (a) adsorbing a protein to Protein A immobilized on a solid phase comprising silica or glass, (b) removing contaminants bound to the solid phase by washing the solid phase with a hydrophobic electrolyte solvent, and (c) recovering the protein from the solid phase.

[0006] WO2011 / 038894 (Patent Document 2) reports a Protein A chromatography method in which host cell proteins and DNA are significantly depleted by special washing steps prior to recovery of immunoglobulins from the Protein A chromatography material.

[0007] WO2013 / 177118 (Patent Document 3) reports compositions and methods for isolating and purifying antibodies from a sample matrix.

[0008] WO2013 / 033517 (Patent Document 4) reports a method for isolating a polypeptide of interest (such as an antibody) from a virus.

[0009] EP2583973 (Patent Document 5) reports a method for purifying a protein, which includes one or more chromatographic processes in which an amino acid, or a dipeptide, oligopeptide, or polyamino acid thereof, is contained in the buffer solutions (equilibration buffer, wash buffer, and elution buffer) used in at least one of the chromatographic processes, thereby purifying a highly pure protein containing extremely small amounts of impurities (e.g., polymers or host cell proteins). [Prior art documents] [Patent documents]

[0010] [Patent Document 1] US 6,127,526 [Patent Document 2] WO2011 / 038894 [Patent Document 3] WO2013 / 177118 [Patent Document 4] WO2013 / 033517 [Patent Document 5] EP2583973 Summary of the Invention

[0011] Herein, a method is reported for producing antibodies with reduced content of host cell proteins by purifying the antibodies by an affinity chromatography step.

[0012] More specifically, it has been found that the method of the present invention, using a low conductivity aqueous solution in the washing step of affinity chromatography prior to recovery of the antibody from the chromatographic material, allows the content of certain host cell proteins in the antibody-containing solution to be reduced. As a result, it has been found that the content of phospholipases, in particular phospholipase B-like 2 (PLBL2), can be reduced. It has been found that the PLBL2 content can be reduced by 100-fold or more when the antibody is of the IgG4 isotype.

[0013] One aspect reported herein is the use of low conductivity aqueous solutions to reduce the content of host cell proteins in the wash steps of Protein A chromatography used to purify human IgG1 or human IgG4 isotype antibodies.

[0014] In one embodiment of this aspect, the human IgG4 isotype antibody is an antibody against P-selectin, or a bispecific antibody against Factor IXa and Factor X, or an antibody against IL-13, or an antibody against amyloid beta. In one embodiment of this aspect, the human IgG1 isotype antibody is an antibody against influenza B, or an antibody against VEGF-A, or an antibody against CD22, or a bispecific antibody against HER3 and EGFR, or an antibody against amyloid beta, or an antibody against Her2, or a bispecific antibody against Ang2 and VEGF-A, or a bispecific antibody against carcinoembryonic antigen (CEA) and CD3.

[0015] In one embodiment of this aspect, the low conductivity aqueous solution has a conductivity value of about 0.5 mS / cm or less.

[0016] In one embodiment of this aspect, the host cell protein is phospholipase B-like 2 (PLBL2) or clusterin.

[0017] In one embodiment of this aspect, the low conductivity aqueous solution contains about 0.1 mM to about 8 mM Tris.

[0018] In one embodiment of this aspect, the low-conductivity aqueous solution contains about 0.05 mM to about 2 mM calcium phosphate.

[0019] In one embodiment of this aspect, the low conductivity aqueous solution has a pH of about 7 or greater.

[0020] In one embodiment of this aspect, a low conductivity aqueous solution washing step is performed after or before a high conductivity aqueous solution washing step.

[0021] In one embodiment of this aspect, the high conductivity aqueous solution has a conductivity value of about 20 mS / cm or greater.

[0022] In one embodiment of this aspect, an intermediate washing step with a medium conductivity aqueous solution is performed between the low conductivity aqueous solution washing step and the high conductivity aqueous solution washing step.

[0023] In one embodiment of this aspect, the medium conductivity aqueous solution has a conductivity value of from greater than 0.5 mS / cm to less than 20 mS / cm.

[0024] In one embodiment of this aspect, the high (or medium) conductivity aqueous solution contains histidine.

[0025] One aspect reported herein is (a) culturing a cell comprising a nucleic acid encoding a human IgG4 isotype antibody or a human IgG1 isotype antibody; (b) recovering human IgG4 isotype antibodies or human IgG1 isotype antibodies from the cells or culture medium; (c) contacting a human IgG4 isotype antibody or a human IgG1 isotype antibody with a Protein A chromatography material; (d) washing the Protein A chromatography material with a low conductivity aqueous solution; (e) recovering human IgG4 isotype antibodies or human IgG1 isotype antibodies from the Protein A chromatography material. Including, thereby producing human IgG4 isotype antibodies or human IgG1 isotype antibodies, A method for producing human IgG4 or human IgG1 isotype antibodies.

[0026] One aspect reported herein is (a) providing a sample containing a human IgG4 isotype antibody or a human IgG1 isotype antibody; (b) purifying human IgG4 isotype antibodies or human IgG1 isotype antibodies by a Protein A chromatography method / step comprising washing the Protein A chromatography material with a low conductivity aqueous solution. The present invention relates to a method for purifying a human IgG4 isotype antibody or a human IgG1 isotype antibody from a sample, the method comprising the steps of:

[0027] In one embodiment of all aspects, the human IgG4 isotype antibody is an antibody against P-selectin, or a bispecific antibody against Factor IXa and Factor X, or an antibody against IL-13, or an antibody against amyloid beta. In one embodiment of all aspects, the human IgG1 isotype antibody is an antibody against influenza B, or an antibody against VEGF-A, or an antibody against CD22, or a bispecific antibody against HER3 and EGFR, or an antibody against amyloid beta, or an antibody against Her2, or a bispecific antibody against Ang2 and VEGF-A, or a bispecific antibody against carcinoembryonic antigen (CEA) and CD3.

[0028] In one embodiment of all aspects, the low conductivity aqueous solution has a conductivity value of about 0.5 mS / cm or less.

[0029] In one embodiment of all aspects the content of host cell proteins is reduced, the (particular) host cell proteins being phospholipase B-like 2 (PLBL2) or clusterin.

[0030] In one embodiment of all aspects, the low conductivity aqueous solution contains about 0.1 mM to about 8 mM Tris.

[0031] In one embodiment of all aspects, the low conductivity aqueous solution contains about 0.05 mM to about 2 mM potassium phosphate.

[0032] In one embodiment of all aspects, the low conductivity aqueous solution has a pH of about 7 or greater.

[0033] In one embodiment of all method aspects, the method further comprises washing the affinity chromatography material with a high conductivity aqueous solution and / or a medium conductivity aqueous solution before or after washing the Protein A chromatography material with the low conductivity aqueous solution.

[0034] In one embodiment of all aspects, the high conductivity aqueous solution has a conductivity value of about 20 mS / cm or greater.

[0035] In one embodiment of all aspects, the medium conductivity aqueous solution has a conductivity value of from greater than 0.5 mS / cm to less than 20 mS / cm.

[0036] In one embodiment of all aspects, the high conductivity or medium conductivity aqueous solution comprises histidine. [The present invention 1001] 1. Use of a low-conductivity aqueous solution having a conductivity value of about 0.5 mS / cm or less to reduce the content of host cell proteins in the wash step of Protein A chromatography used to purify human IgG4 isotype antibodies or human IgG1 isotype antibodies. [The present invention 1002] The use of the present invention 1001, wherein the host cell protein is phospholipase B-like 2 (PLBL2) or clusterin. [The present invention 1003] The use of any one of 1001 to 1002 of the present invention, wherein the low-conductivity aqueous solution contains about 0.1 mM to about 8 mM Tris. [The present invention 1004] Use of any one of 1001 to 1002 of the present invention, wherein the low conductivity aqueous solution contains about 0.05 mM to about 2 mM potassium phosphate. [The present invention 1005] Use of any of the present inventions 1001 to 1004, wherein the low conductivity aqueous solution has a pH of about 7 or more. [The present invention 1006] The use of any of the inventions 1001-1005, wherein a low conductivity aqueous solution cleaning step is performed after or before a high conductivity aqueous solution cleaning step. [The present invention 1007] The use of the present invention 1006, wherein the high conductivity aqueous solution has a conductivity value of about 20 mS / cm or greater. [The present invention 1008] Use of any one of claims 1006 to 1007, wherein the high conductivity aqueous solution contains histidine. [The present invention 1009] The use of any of the present inventions 1001 to 1008, wherein the human IgG4 isotype antibody is an antibody against P-selectin, or an antibody against factors IXa and X, or an antibody against IL-13, or an antibody against amyloid beta. [The present invention 1010] The use of any of the inventions 1001 to 1008, wherein the human IgG1 isotype antibody is an antibody against influenza B, or an antibody against VEGF-A, or an antibody against CD22, or a bispecific antibody against HER3 and EGFR, or an antibody against amyloid beta, or an antibody against Her2, or a bispecific antibody against Ang2 and VEGF-A, or a bispecific antibody against carcinoembryonic antigen (CEA) and CD3. [The present invention 1011] (a) culturing a cell comprising a nucleic acid encoding a human IgG4 isotype antibody or a human IgG1 isotype antibody; (b) recovering human IgG4 isotype antibodies or human IgG1 isotype antibodies from the cells or culture medium; (c) contacting a human IgG4 isotype antibody or a human IgG1 isotype antibody with a Protein A chromatography material; (d) washing the Protein A chromatography material with a low-conductivity aqueous solution having a conductivity value of about 0.5 mS / cm or less; (e) recovering human IgG4 isotype antibodies or human IgG1 isotype antibodies from the Protein A chromatography material. Including, thereby producing human IgG4 isotype antibodies or human IgG1 isotype antibodies, A method for producing human IgG4 or human IgG1 isotype antibodies. [The present invention 1012] (a) providing a sample containing a human IgG4 isotype antibody or a human IgG1 isotype antibody; (b) purifying human IgG4 isotype antibodies or human IgG1 isotype antibodies by a Protein A chromatography method / step comprising washing the Protein A chromatography material with a low-conductivity aqueous solution having a conductivity value of about 0.5 mS / cm or less. 1. A method for purifying a human IgG4 isotype antibody or a human IgG1 isotype antibody from a sample, comprising: [The present invention 1013] The method of any of claims 1011 to 1012, wherein the amount of a host cell protein is reduced, and the host cell protein is phospholipase B-like 2 (PLBL2) or clusterin. [The present invention 1014] The method of any one of claims 1011 to 1013, wherein the low-conductivity aqueous solution contains about 0.1 mM to about 8 mM Tris. [The present invention 1015] The method according to any one of claims 1011 to 1014, wherein the low-conductivity aqueous solution contains about 0.05 mM to about 2 mM potassium phosphate. [The present invention 1016] The method of any one of claims 1011 to 1015, wherein the low-conductivity aqueous solution has a pH of about 7 or more. [The present invention 1017] Any of the methods of claims 1011 to 1016, further comprising a step of washing the affinity chromatography material with a high conductivity aqueous solution and / or a medium conductivity aqueous solution before or after washing the Protein A chromatography material with a low conductivity aqueous solution. [The present invention 1018] The method of claim 1017, wherein the high conductivity aqueous solution has a conductivity value of about 20 mS / cm or greater. [The present invention 1019] The method of claim 1017, wherein the medium conductivity aqueous solution has a conductivity value of from greater than 0.5 mS / cm to less than 20 mS / cm. [The present invention 1020] The method according to any one of claims 1017 to 1019, wherein the high conductivity aqueous solution or the medium conductivity aqueous solution contains histidine. [The present invention 1021] The method according to any one of claims 1011 to 1020, wherein the human IgG4 isotype antibody is an antibody against P-selectin, or an antibody against factors IXa and X, or an antibody against IL-13, or an antibody against amyloid beta. [The present invention 1022] The method of any of claims 1011 to 1020, wherein the human IgG1 isotype antibody is an antibody against influenza B, or an antibody against VEGF-A, or an antibody against CD22, or a bispecific antibody against HER3 and EGFR, or an antibody against amyloid beta, or an antibody against Her2, or a bispecific antibody against Ang2 and VEGF-A, or a bispecific antibody against carcinoembryonic antigen (CEA) and CD3. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0037] Detailed Description of the Invention Reported herein are improved affinity chromatography methods and uses that involve washing the affinity chromatography material with low conductivity aqueous solutions.

[0038] It has been found that the use of a low-conductivity aqueous solution wash step in an affinity chromatography step, such as a Protein A chromatography step, allows the reduction of certain host cell proteins. The affinity chromatography step is used in a method for purifying or producing an antibody. The low-conductivity aqueous solution wash step is particularly effective in reducing the content of phospholipase B-like 2 (PLBL2).

[0039] One aspect reported herein is the use of low conductivity aqueous solutions to reduce the content of (certain) host cell proteins in the wash steps of affinity chromatography.

[0040] One aspect reported herein is (a) culturing cells containing nucleic acid encoding a human IgG isotype antibody; (b) recovering human IgG isotype antibodies from the cells or culture medium; (c) contacting (a solution containing) a human IgG isotype antibody with an affinity chromatography material; (d) washing the affinity chromatography material with a low conductivity aqueous solution while maintaining at least 90% of the bispecific antibody bound to the affinity chromatography material; (e) recovering human IgG isotype antibodies from the affinity chromatography material. Including, thereby producing human IgG isotype antibodies, A method for producing human IgG isotype antibodies.

[0041] One aspect reported herein is (a) providing a (buffered aqueous) sample containing human IgG isotype antibodies; (b) purifying human IgG isotype antibodies by an affinity chromatography method / step comprising washing the affinity chromatography material with a low conductivity aqueous solution. A method for purifying human IgG isotype antibodies from a sample, comprising:

[0042] Recombinant polypeptides produced in CHO cells can be purified according to methods described herein to remove or reduce the levels of host cell proteins.

[0043] Exemplary recombinant polypeptides include therapeutic antibodies and immunoadhesins, including, but not limited to, antibodies (including antibody fragments) against one or more of the following antigens: HER1 (EGFR), HER2 (e.g., trastuzumab, pertuzumab), HER3, HER4, VEGF (e.g., bevacizumab, ranibizumab), MET (e.g., onartuzumab), CD20 (e.g., rituximab, obinutuzumab, ocrelizumab), CD22, CD11a, CD11b, CD11c, CD18, ICAM, VLA-4, VCAM, IL-17A and / or F, IgE (e.g., omalizumab), DRS, CD40, Apo2L / TRAIL, EGFL7 (e.g., parsatuzumab), NRP1, integrin beta 7 (e.g., etrolizumab), IL-13 (e.g., leuconazole), IL-14 (e.g., leuconazole), IL-15 (e.g., leuconazole), IL-16 (e.g., leuconazole), IL-17 (e.g., leuconazole), IL-18 (e.g., leuconazole), IL-19 (e.g., leuconazole), IL-20 (e.g., leuconazole), IL-21 (e.g., leuconazole), IL-22 (e.g., leuconazole), IL-23 (e.g., leuconazole), IL-24 (e.g., leuconazole), IL-25 (e.g., leuconazole), IL-26 (e.g., leuconazole), IL-27 (e.g., leuconazole kizumab), Abeta (e.g. crenezumab, gantenerumab), P-selectin (e.g. inlacumab), IL-6R (e.g. tosiluzumab), IFNa (e.g. rontalizumab), M1prime (e.g. quilizumab), mitogen-activated protein kinase (MAPK), OX40L, TSLP, factor D (e.g. lampalizumab), and receptors such as IL-9 receptor, IL-5 receptor, IL-4 receptor alpha, IL-13 receptor alpha 1 and IL-13 receptor alpha 2, OX40, TSLP-R, IL-7R alpha (coreceptor for TSLP), IL17RB (receptor for IL-25), ST2 (receptor for IL-33), CCR3, CCR4, CRTH2, Fc epsilon RI and Fc epsilon RII / CD23 (receptor for IgE).Other exemplary antibodies include, but are not limited to, anti-estrogen receptor antibodies, anti-progesterone receptor antibodies, anti-p53 antibodies, anti-cathepsin D antibodies, anti-Bcl-2 antibodies, anti-E-cadherin antibodies, anti-CA125 antibodies, anti-CA15-3 antibodies, anti-CA19-9 antibodies, anti-c-erbB-2 antibodies, anti-P-glycoprotein antibodies, anti-CEA antibodies, Ki-67 antibodies, anti-PCNA antibodies, anti-CD3 antibodies, anti-CD4 antibodies, anti-CD5 antibodies, anti-CD7 antibodies, anti-CD8 antibodies, anti-CD9 / p24 antibodies, anti-CD10 antibodies, anti-CD11c antibodies, anti-CD13 antibodies, anti-CD14 antibodies, anti-CD15 antibodies, anti-CD19 antibodies, anti-CD23 antibodies, anti-CD30 antibodies, anti-CD3 1 antibody, anti-CD33 antibody, anti-CD34 antibody, anti-CD35 antibody, anti-CD38 antibody, anti-CD41 antibody, anti-LCA / CD45 antibody, anti-CD45RO antibody, anti-CD45RA antibody, anti-CD39 antibody, anti-CD100 antibody, anti-CD95 / Fas antibody, anti-CD99 antibody, anti-CD106 antibody, anti-ubiquitin antibody, anti-CD71 antibody, anti-c-myc antibody, anti-cytokeratin antibody, anti-vimentin antibody, anti-HPV protein antibody, anti-kappa light chain antibody, anti-lambda light chain antibody, anti-melanosome antibody, anti-prostate specific antigen antibody, anti-S-100 antibody, anti-tau antigen antibody, anti-fibrin antibody, anti-keratin antibody and anti-Tn antigen antibody.

[0044] In some embodiments, exemplary antibodies include antibodies against Abeta, antibodies against IL17 A / F, and antibodies against CMV. Exemplary anti-Abeta antibodies and methods for producing such antibodies have been previously described, for example, in WO2008011348, WO2007068429, WO2001062801, and WO2004071408. Exemplary anti-IL17 A / F antibodies and methods for producing such antibodies have been previously described, for example, in WO2009136286 and U.S. Patent No. 8,715,669. Exemplary anti-CMV antibodies, including anti-CMV-MSL, and methods for producing such antibodies have been previously described, for example, in WO2012047732.

[0045] In some embodiments, affinity chromatography is used to purify human IgG isotype antibodies. In some embodiments, affinity chromatography is used to purify IgG4 antibodies. In one embodiment, the IgG4 isotype antibody is an antibody against P-selectin, or a (bispecific) antibody against factor IXa and factor X, or an antibody against IL-13, or an antibody against amyloid beta. In some embodiments, affinity chromatography is used to purify IgG1 isotype antibodies. In one embodiment, the IgG1 isotype antibody is an antibody against influenza B, or an antibody against VEGF-A, or an antibody against CD22, or a (bispecific) antibody against HER3 and EGFR, or an antibody against amyloid beta, or an antibody against Her2, or a bispecific antibody against Ang2 and VEGF-A, or a bispecific antibody against carcinoembryonic antigen (CEA) and CD3.

[0046] One aspect reported herein is (a) culturing cells containing nucleic acids encoding human IgG4 isotype antibodies; (b) recovering human IgG4 isotype antibodies from the cells or culture medium; (c) contacting a human IgG4 isotype antibody with an affinity chromatography material; (d) washing the affinity chromatography material with a low conductivity aqueous solution; (e) recovering human IgG4 isotype antibodies from the affinity chromatography material. Including, Thereby producing human IgG4 isotype antibodies. A method for producing a human IgG4 isotype antibody (containing solution).

[0047] One aspect reported herein is (a) culturing cells containing nucleic acid encoding an IgG4 isotype antibody; (b) recovering the IgG4 isotype antibody from the cells or culture medium; (c) contacting the IgG4 isotype antibody with an affinity chromatography material; (d) washing the affinity chromatography material with a low conductivity aqueous solution; (e) recovering IgG4 isotype antibodies from the affinity chromatography material. Including, This produces IgG4 isotype antibodies. A method for producing an IgG4 isotype antibody (containing solution).

[0048] One aspect reported herein is (a) providing a sample containing a human IgG4 isotype antibody; (b) purifying the human IgG4 isotype antibody by an affinity chromatography method / step comprising washing the affinity chromatography material with a low conductivity aqueous solution. A method for purifying human IgG4 isotype antibodies from a sample, comprising:

[0049] One aspect reported herein is (a) providing a sample containing an IgG4 isotype antibody; (b) purifying the IgG4 isotype antibody by an affinity chromatography method / step comprising washing the affinity chromatography material with a low conductivity aqueous solution. A method for purifying IgG4 isotype antibodies from a sample, comprising:

[0050] It has been found that the content of host cell proteins can be reduced if the conductivity of the aqueous solution used in the washing step is low, i.e. if a low conductivity aqueous solution is used for washing. In one embodiment of all aspects, the low conductivity aqueous solution has a conductivity value of about 1 mS / cm or less. In a preferred embodiment of all aspects, the low conductivity aqueous solution has a conductivity value of about 0.5 mS / cm or less. In one embodiment, the low conductivity aqueous solution has a conductivity value of about 0.03 μS / cm to about 0.5 mS / cm. In one embodiment, the low conductivity aqueous solution has a conductivity value of about 0.05 μS / cm to about 0.35 mS / cm. In one embodiment of all aspects, the low conductivity aqueous solution is deionized water. Deionized water is not suitable for use in the washing step for some applications. In some embodiments, the low conductivity aqueous solution is not deionized water.

[0051] It has been found that Protein A affinity chromatography can be used for the purposes reported herein. In a preferred embodiment of all aspects, the affinity chromatography is Protein A affinity chromatography. In one embodiment, the Protein A affinity chromatography is selected from the group comprising MabSelectSure affinity chromatography, ProSep vA affinity chromatography, Poros Mab Capture A affinity chromatography, ProSep Ultra Plus affinity chromatography, MabSelect SuRe LX, MabSelect, Eshmuno A, Toyopearl AF-rProtein A-650F; Toyopearl AF-rProtein A HC-650HF). In one embodiment, the affinity chromatography is Protein G affinity chromatography. In one embodiment, the affinity chromatography is affinity chromatography using recombinant protein as a ligand, i.e. recombinant protein ligand affinity chromatography. In one embodiment, the affinity chromatography is affinity chromatography using single chain Fv as a ligand, i.e. single chain Fv ligand affinity chromatography. In one embodiment, the affinity chromatography comprises a mutant Protein A coupled to a chromatography matrix, or a fragment of Protein A coupled to a chromatography matrix.

[0052] It has been found that the content of (specific) host cell proteins can be reduced. In particular, it has been found that the content of phospholipase B-like 2 (PLBL2) can be reduced. In one embodiment, the (specific) host cell protein is a Chinese Hamster Ovary (CHO) host cell protein. In a preferred embodiment of all aspects, the (specific) host cell protein is phospholipase B-like 2 (PLBL2) or clusterin. In one embodiment, the (specific) host cell protein is phospholipase B-like 2 (PLBL2).

[0053] It has been found that the low-conductivity aqueous solution may contain certain buffer substances, such as small amounts of Tris or potassium phosphate. In one embodiment, the low-conductivity aqueous solution contains tris(hydroxymethyl)aminomethane (Tris). In one embodiment, the low-conductivity aqueous solution contains about 0.1 mM to about 10 mM Tris. In one embodiment, the low-conductivity aqueous solution contains about 0.5 mM to about 6.5 mM Tris. In one embodiment, the low-conductivity aqueous solution contains about 2 mM Tris. In one embodiment, the low-conductivity aqueous solution contains potassium phosphate. In one embodiment, the low-conductivity aqueous solution contains about 0.05 mM to about 5 mM potassium phosphate. In one embodiment, the low-conductivity aqueous solution contains about 0.05 mM to about 2 mM potassium phosphate. In one embodiment, the low-conductivity aqueous solution contains about 0.5 mM potassium phosphate.

[0054] It has been found that the effect of reducing the host cell protein content is remarkable when the low-conductivity aqueous solution has a certain pH. In one embodiment, the low-conductivity aqueous solution has a pH of about 7 or more. In one embodiment, the low-conductivity aqueous solution has a pH of about 7.5 or more. In one embodiment, the low-conductivity aqueous solution has a pH of about 7 to about 9.5. In one embodiment, the low-conductivity aqueous solution has a pH of about 7.5 to about 8.5. In one embodiment, the low-conductivity aqueous solution has a pH of about 8. In one embodiment, the low-conductivity aqueous solution has a pH of about 9.

[0055] It has been found that the effect of reducing the content of host cell proteins can also be achieved when the pH of the low-conductivity aqueous solution is about 8.5 or more and the low-conductivity aqueous solution has a conductivity value of about 1.2 mS / cm or less. In one embodiment, the low-conductivity aqueous solution has a pH of about 8.5 or more and a conductivity value of about 1.2 mS / cm or less. In one embodiment, the low-conductivity aqueous solution has a pH of about 8.5 or more and a conductivity value of about 1 mS / cm or less. In one embodiment, the low-conductivity aqueous solution has a pH of about 8.5 or more and the low-conductivity aqueous solution contains about 55 mM Tris or less. In one embodiment, the low-conductivity aqueous solution has a pH of about 8.5 or more and the low-conductivity aqueous solution contains about 30 mM Tris or less.

[0056] In one embodiment, the low conductivity aqueous solution has a conductivity value of about 0.5 mS / cm or less in the pH range from pH 7 to less than pH 8.5, and a conductivity value of about 1.2 mS / cm or less at pH values ​​of 8.5 or greater.

[0057] It has been found that the use and methods reported herein can reduce the content of host cell proteins such as PLBL2 to a certain level compared to the loading of PLBL2 before a purification step such as an affinity chromatography step. In one embodiment, the content of PLBL2 is reduced at least 20-fold. In one embodiment, the content of PLBL2 is reduced at least 40-fold. In one embodiment, the content of PLBL2 is reduced at least 50-fold. In one embodiment, the content of PLBL2 is reduced at least 90-fold. In one embodiment, the content of PLBL2 is reduced at least 100-fold. In some cases, the level of reduction is even higher. In some embodiments, the content of PLBL2 is reduced at least 200-fold. In some embodiments, the content of PLBL2 is reduced at least 250-fold. In some embodiments, the content of PLBL2 is reduced at least 300-fold. In some embodiments, the content of PLBL2 is reduced at least 400-fold. In some embodiments, the content of PLBL2 is reduced at least 1000-fold. In one embodiment, the content of PLBL2 is reduced by at least 50%. In one embodiment, the content of PLBL2 is reduced by at least 66%. In one embodiment, the content of PLBL2 is reduced by at least 80%. In one embodiment, the content of PLBL2 is reduced by at least 90%. In one embodiment, the content of PLBL2 is reduced by at least 95%. In some embodiments, the content of PLBL2 is reduced to less than 10 ng / mg of antibody. In some embodiments, the content of PLBL2 is reduced to less than 5 ng / mg of antibody. In some embodiments, the content of PLBL2 is reduced to less than 2 ng / mg of antibody.

[0058] In the methods and uses reported herein, further washing steps with medium conductivity aqueous solutions and / or high conductivity aqueous solutions can be used. In one embodiment, a low conductivity aqueous solution washing step is performed after or before a high conductivity aqueous solution washing step. In one embodiment, the high conductivity aqueous solution has a conductivity value of about 20 mS / cm or more. In one embodiment, the high conductivity aqueous solution has a conductivity value of about 20 mS / cm to about 100 mS / cm. In one embodiment, between the low conductivity aqueous solution washing step and the high conductivity aqueous solution washing step, an intermediate washing step with a medium conductivity aqueous solution is performed. In one embodiment, the medium conductivity aqueous solution has a conductivity value of more than 0.5 mS / cm to less than 20 mS / cm.

[0059] It has been found that the host cell protein reduction effect can be improved when the high conductivity aqueous solution or the medium conductivity aqueous solution further contains an amino acid. In one embodiment, the high conductivity aqueous solution or the medium conductivity aqueous solution contains an amino acid. In one embodiment, the high conductivity aqueous solution or the medium conductivity aqueous solution contains histidine or arginine. In one embodiment, the high conductivity aqueous solution or the medium conductivity aqueous solution contains histidine. In one embodiment, the high conductivity aqueous solution or the medium conductivity aqueous solution contains histidine and Tris.

[0060] The methods and uses reported herein may comprise one or more further chromatography steps. In one embodiment, at least one additional chromatography method / step is performed. In one embodiment, an additional ion exchange chromatography method / step is performed. In one embodiment, an additional anion exchange chromatography method / step is performed. In one embodiment, an additional anion exchange chromatography method / step and an additional cation exchange chromatography method / step are performed.

[0061] It has been found that the use of a hydrophobic interaction chromatography step may be omitted. In one embodiment, the use or method does not involve a hydrophobic interaction chromatography method / step.

[0062] One aspect reported herein is the use of low-conductivity aqueous solutions having a conductivity value of about 0.5 mS / cm or less and a pH of about 7 or greater to reduce the content of PLBL2 or clusterin in the wash steps of Protein A chromatography used to purify IgG4 or IgG1 isotype antibodies, such as human IgG4 or human IgG1 antibodies.

[0063] One aspect is the use of a low conductivity aqueous solution having a conductivity value of about 0.5 mS / cm or less and a pH of about 7 or more to reduce the content of PLBL2 or clusterin in the washing step of protein A chromatography used to purify human IgG4 isotype antibodies or human IgG1 isotype antibodies. In some embodiments, the antibody is an IgG4 isotype antibody, such as an antibody against P-selectin, or a bispecific antibody against factor IXa and factor X, or an antibody against IL-13, or an antibody against amyloid beta. In some embodiments, the antibody is an IgG1 isotype antibody, such as an antibody against influenza B, or an antibody against VEGF-A, or an antibody against CD22, or a bispecific antibody against HER3 and EGFR, or an antibody against amyloid beta, or an antibody against Her2, or a bispecific antibody against Ang2 and VEGF-A, or a bispecific antibody against carcinoembryonic antigen (CEA) and CD3.

[0064] In one aspect, the present disclosure provides a method for producing a method for manufacturing a semiconductor device comprising: (a) culturing a cell comprising a nucleic acid encoding a human IgG4 isotype antibody or a human IgG1 isotype antibody; (b) recovering human IgG4 isotype antibodies or human IgG1 isotype antibodies from the cells or culture medium; (c) contacting a human IgG4 isotype antibody or a human IgG1 isotype antibody with a Protein A affinity chromatography material; (d) washing the Protein A affinity chromatography material with a low conductivity aqueous solution; (e) recovering the human IgG4 isotype antibodies or the human IgG1 isotype antibodies from the affinity chromatography material. Including, thereby producing human IgG4 isotype antibodies or human IgG1 isotype antibodies, 1. A method for producing a human IgG4 or IgG1 isotype antibody, comprising: The method further comprises the steps of: providing a solution of low conductivity aqueous solution having a conductivity value of about 0.5 mS / cm or less and a pH of about 7 or greater.

[0065] In one aspect, the present disclosure provides a method for producing a method for manufacturing a semiconductor device comprising: (a) culturing a cell comprising a nucleic acid encoding a human IgG4 isotype antibody or a human IgG1 isotype antibody; (b) recovering human IgG4 isotype antibodies or human IgG1 isotype antibodies from the cells or culture medium; (c) contacting a human IgG4 isotype antibody or a human IgG1 isotype antibody with a Protein A affinity chromatography material; (d) washing the Protein A affinity chromatography material with a low conductivity aqueous solution; (e) recovering the human IgG4 isotype antibodies or the human IgG1 isotype antibodies from the affinity chromatography material. Including, thereby producing human IgG4 isotype antibodies or human IgG1 isotype antibodies, 1. A method for producing a human IgG4 or IgG1 isotype antibody, comprising: The low-conductivity aqueous solution has a conductivity value of about 0.5 mS / cm or less and a pH of about 7 or greater; The human IgG4 isotype antibody is an antibody against P-selectin, or a bispecific antibody against factor IXa and factor X, or an antibody against IL-13, or an antibody against amyloid beta, and the human IgG1 isotype antibody is an antibody against influenza B, or an antibody against VEGF-A, or an antibody against CD22, or a bispecific antibody against HER3 and EGFR, or an antibody against amyloid beta, or an antibody against Her2, or a bispecific antibody against Ang2 and VEGF-A, or a bispecific antibody against carcinoembryonic antigen (CEA) and CD3. The method is provided.

[0066] In one aspect, the present disclosure provides a method for producing a method for manufacturing a semiconductor device comprising: (a) providing a sample containing a human IgG4 isotype antibody or a human IgG1 isotype antibody; (b) purifying human IgG4 isotype antibodies or human IgG1 isotype antibodies by a Protein A affinity chromatography method / step comprising washing the Protein A affinity chromatography material with a low conductivity aqueous solution. 1. A method for purifying human IgG4 or human IgG1 isotype antibodies from a sample, comprising: The method provides that the low conductivity aqueous solution has a conductivity value of about 0.5 mS / cm or less and a pH of about 7 or greater.

[0067] In one aspect, the present disclosure provides a method for producing a method for manufacturing a semiconductor device comprising: (a) providing a sample containing a human IgG4 isotype antibody or a human IgG1 isotype antibody; (b) purifying human IgG4 isotype antibodies or human IgG1 isotype antibodies by a Protein A affinity chromatography method / step comprising washing the Protein A affinity chromatography material with a low conductivity aqueous solution. 1. A method for purifying human IgG4 or human IgG1 isotype antibodies from a sample, comprising: The low-conductivity aqueous solution has a conductivity value of about 0.5 mS / cm or less and a pH of about 7 or greater; The human IgG4 isotype antibody is an antibody against P-selectin, or an antibody against factor IXa and factor X, or an antibody against IL-13, or an antibody against amyloid beta, and the human IgG1 isotype antibody is an antibody against influenza B, or an antibody against VEGF-A, or an antibody against CD22, or an antibody against HER3 and EGFR, or an antibody against amyloid beta, or an antibody against Her2, or a bispecific antibody against Ang2 and VEGF-A, or a bispecific antibody against carcinoembryonic antigen (CEA) and CD3. The method is provided.

[0068] In one aspect, the present disclosure provides a method for producing a method for manufacturing a semiconductor device comprising: (a) culturing cells containing nucleic acids encoding human IgG4 isotype antibodies; (b) recovering human IgG4 isotype antibodies from the cells or culture medium; (c) contacting a human IgG4 isotype antibody with a Protein A affinity chromatography material; (d) washing the Protein A affinity chromatography material with a low conductivity aqueous solution; (e) recovering human IgG4 isotype antibodies from the affinity chromatography material. Including, Thereby producing human IgG4 isotype antibodies. 1. A method for producing a human IgG4 isotype antibody, comprising: The low-conductivity aqueous solution has a conductivity value of about 0.5 mS / cm or less and a pH of about 7 or greater; The human IgG4 isotype antibody is directed against factors IXa and X. The method is provided.

[0069] In one aspect, the present disclosure provides a method for producing a method for manufacturing a semiconductor device comprising: (a) providing a sample containing a human IgG4 isotype antibody; (b) purifying human IgG4 isotype antibodies by a Protein A affinity chromatography method / step comprising washing the Protein A affinity chromatography material with a low conductivity aqueous solution; 1. A method for purifying human IgG4 isotype antibodies from a sample, comprising: The low-conductivity aqueous solution has a conductivity value of about 0.5 mS / cm or less and a pH of about 7 or greater; The human IgG4 isotype antibody is directed against factors IXa and X. The method is provided.

[0070] The terms "anti-P-selectin antibody" and "antibody that binds to P-selectin" or "antibody against P-selectin" refer to an antibody that has the ability to bind to P-selectin with sufficient affinity such that the antibody serves as a diagnostic and / or therapeutic agent in targeting P-selectin. In one embodiment, the extent of binding of the anti-P-selectin antibody to an unrelated non-P-selectin protein is less than about 10% of the binding of the antibody to P-selectin, as measured, for example, by ELISA or surface plasmon resonance. In certain embodiments, the anti-P-selectin antibody binds to an epitope of P-selectin that is conserved among P-selectins from different species. The above also applies to terms such as "antibody against factor IXa and factor X" or "antibody against IL-13" or "antibody against amyloid beta".

[0071] In some embodiments, the antibody against P-selectin is inlacumab (IgG4 isotype) as described in WO2005 / 100402 or SEQ ID NOs:07-12. In some embodiments, the antibody is a bispecific antibody against factor IXa and factor X, such as the anti-FIXa / X antibody (IgG4 isotype) as described in WO2012 / 067176. In some embodiments, the antibody is an antibody against Her2, such as trastuzumab (IgG1 isotype) as described in WO1992 / 022653. In some embodiments, the antibody is a bispecific antibody against angiopoietin 2 (Ang2) and vascular endothelial growth factor A (VEGF-A), such as vanucizumab (IgG1 isotype) as described in WO2011 / 117329 or SEQ ID NOs:01-04. In some embodiments, the antibody is an antibody against amyloid beta, such as gantenerumab (IgG1 isotype) as described in WO2003 / 070760 or SEQ ID NO:05-06, or crenezumab (IgG4 isotype). In some embodiments, the antibody is an antibody against CD22, an antibody against IL13 (e.g., lebrikizumab), a bispecific antibody against Her3 and EGFR (e.g., duligotuzumab), an antibody against VEGF-A (e.g., bevacizumab), and an antibody against influenza B. The terms VEGF or VEGF-A may be used interchangeably herein.

[0072] As used herein, the terms "bind" or "specifically bind" refer to the binding of an antibody to an epitope of an antigen in an in vitro assay, preferably in a surface plasmon resonance assay (SPR, BIAcore, GE-Healthcare, Uppsala, Sweden). The affinity of the binding is measured using the terms ka (rate constant for the association of the antibody with the antibody / antigen complex), k d (dissociation constant), and K D (k d / k a ) is defined as binding or specifically binding. -7Binding affinity (K D )

[0073] The term "antibody" is used herein in the broadest sense and encompasses a variety of antibody structures, including but not limited to monoclonal antibodies, polyclonal antibodies, multispecific antibodies (e.g., bispecific antibodies), and antibody fragments, so long as they exhibit the desired antigen-binding activity.

[0074] "Antibody fragment" refers to a molecule other than an intact antibody that contains a portion of the intact antibody that binds to the antigen to which the intact antibody binds. Examples of antibody fragments include, but are not limited to, Fv, Fab, Fab', Fab'-SH, F(ab')2; diabodies; linear antibodies; single-chain antibody molecules (e.g., scFv); and multispecific antibodies formed from antibody fragments. Fab fragments are antibody fragments obtained by papain digestion of (full-length / complete) antibodies.

[0075] A "bispecific antibody" is an antibody having two different antigen-binding specificities. As used herein, the term "bispecific" antibody refers to an antibody having at least two binding sites, each of which binds to a different epitope.

[0076] The term "chimeric" antibody refers to an antibody in which a portion of the heavy and / or light chain is derived from a particular source or species, while the remaining portions of the heavy and / or light chain are derived from a different source or species.

[0077] The "class" of an antibody refers to the type of constant domain or constant region carried by its heavy chain. There are five major classes of antibodies, namely IgA, IgD, IgE, IgG, and IgM, some of which can be further divided into subclasses (isotypes), e.g., IgG1, IgG2, IgG3, IgG4, IgA1, and IgA2. The heavy chain constant domains that correspond to the different classes of immunoglobulins are called α, δ, ε, γ, and μ, respectively.

[0078] The term "human IgG isotype antibody" refers to an antibody that comprises a constant region derived from the human wild-type IgG isotype, i.e. it may comprise a constant region derived from the human IgG isotype, for example with a mutation, for example the P329G mutation (numbering according to Kabat).

[0079] The term "human IgG4 isotype antibody" refers to an antibody comprising a constant region derived from the human wild-type IgG4 isotype, i.e. it may comprise a constant region derived from the human IgG4 isotype, for example with mutations such as the P329G mutation and / or the S228P, L235E mutations (numbering according to Kabat).

[0080] The term "Fc region" is used herein to define a C-terminal region of an immunoglobulin heavy chain that contains at least a portion of the constant region. This term encompasses native sequence Fc regions and variant Fc regions. In one embodiment, a human IgG heavy chain Fc region extends from Cys226 or Pro230 of the heavy chain to the carboxyl terminus, with the exception that the C-terminal lysine (Lys447) or the C-terminal glycyl-lysine dipeptide (Gly446Lys447) of the Fc region may or may not be present. Unless otherwise specified, the numbering of amino acid residues in the Fc region or constant region is according to the EU numbering system, also referred to as the EU index, as described in Kabat, EA et al, Sequences of Proteins of Immunological Interest, 5th ed., National Institutes of Health Public Health Service, Bethesda, MD (1991), NIH Publication 91-3242.

[0081] "Framework" or "FR" refers to variable domain residues other than hypervariable region (HVR) residues. The FR of a variable domain generally consists of four FR domains, namely FR1, FR2, FR3, and FR4. Thus, the HVR and FR sequences generally appear in the following order in a VH (or VL): FR1-H1(L1)-FR2-H2(L2)-FR3-H3(L3)-FR4.

[0082] The terms "host cell", "host cell line" and "host cell culture" are used interchangeably and refer to cells into which exogenous nucleic acid has been introduced, including the progeny of such cells. Host cells include "transformants" and "transformed cells", including the primary transformed cell and progeny derived therefrom, regardless of the number of passages. Progeny may not be completely identical in nucleic acid content to the parent cell and may contain mutations. Mutant progeny that have the same function or biological activity as screened or selected in the originally transformed cell are included herein. The term "cell" includes cells used for expression of nucleic acids. In one embodiment, the host cell is a CHO cell (e.g., CHO K1, CHO DG44), or a BHK cell, or an NS0 cell, or an SP2 / 0 cell, or an HEK293 cell, or an HEK293EBNA cell, or a PER.C6® cell, or a COS cell. In another embodiment, the cell is a CHO cell, or a BHK cell, or a PER.C6® cell. As used herein, the term "cell" encompasses the subject cell and its progeny.

[0083] The term "washing" refers to the application of a solution to an affinity chromatography material to remove non-specifically bound polypeptide and non-polypeptide compounds from the chromatography material, particularly to remove host cell proteins and host cell DNA. The term "washing" does not include the elution of bound material from the affinity chromatography material.

[0084] For the recovery and purification of proteins, various methods have been established and are widely used, such as affinity chromatography with microbial proteins (e.g., Protein A or Protein G affinity chromatography), affinity chromatography with recombinant proteins as ligands (e.g., single-chain Fv, e.g., Kappa select), ion exchange chromatography (e.g., cation exchange (carboxymethyl resin), anion exchange (aminoethyl resin) and mixed mode exchange), thiophilic adsorption (e.g., with beta-mercaptoethanol ligands and other SH ligands), hydrophobic interaction or aromatic adsorption chromatography (e.g., with phenyl-sepharose, aza-arenophilic resin, or m-aminophenylboronic acid), metal chelate affinity chromatography (e.g., with Ni(II)-affinity material and Cu(II)-affinity material), size exclusion chromatography, and electrophoretic methods (gel electrophoresis, capillary electrophoresis, etc.). These methods can be combined independently in various embodiments, as reported herein.

[0085] The term "Protein A" refers to a Protein A polypeptide, whether obtained from natural sources or produced synthetically.

[0086] The term "Protein A chromatography material" refers to an inert solid phase to which Protein A is covalently attached.

[0087] In one embodiment, the Protein A chromatography material is selected from MabSelectSure, ProSep vA, Mab Capture A, ProSep Ultra Plus, Mab Select, Mab Select Xtra, Poros A, or ProSep A.

[0088] The term "high conductivity aqueous solution" refers to an aqueous solution having a high conductivity value, which may be about 20 mS / cm or greater.

[0089] The term "medium conductivity aqueous solution" refers to an aqueous solution having an intermediate conductivity value, which can be greater than 0.5 mS / cm and less than 20 mS / cm.

[0090] The term "low conductivity aqueous solution" refers to an aqueous solution having a low conductivity value. The conductivity value may be about 0.5 mS / cm or less. If the pH is about 8.5 or more, the conductivity value may be about 1.2 mS / cm or less. The conductivity value may be measured by standard methods known to those skilled in the art.

[0091] The following examples and sequences are provided to aid the understanding of the present invention, the true scope of which is set forth in the appended claims. It is understood that modifications can be made in the procedures set forth without departing from the spirit or scope of the invention.

[0092] Specific Aspects of the Invention 1. The use of low conductivity aqueous solutions to reduce the content of host cell proteins in the wash steps of affinity chromatography. 2. The use of embodiment 1, wherein affinity chromatography is used to purify human IgG isotype antibodies. 3. The use of embodiment 2, wherein affinity chromatography is used to purify human IgG4 isotype antibodies or human IgG1 isotype antibodies. 4. Use of embodiment 3, wherein affinity chromatography is used to purify a human IgG4 isotype antibody or a human IgG1 isotype antibody having no / exactly one glycosylation site in its Fab fragment (at position Asn297 according to the Kabat numbering). 5. The use of embodiment 4, wherein the low-conductivity aqueous solution has a conductivity value of about 0.5 mS / cm or less. 6. The use of embodiment 5, wherein the low conductivity aqueous solution has a conductivity value of about 0.03 μS / cm to about 0.5 mS / cm. 7. The use of embodiment 5, wherein the low conductivity aqueous solution has a conductivity value of about 0.05 μS / cm to about 0.35 mS / cm. 8. The use of any one of embodiments 5-7, wherein the low-conductivity aqueous solution is not deionized water. 9. The use of any one of the previous embodiments, wherein the affinity chromatography is Protein A affinity chromatography or Protein G affinity chromatography or single chain Fv ligand (KappaSelect) affinity chromatography. 10. The use of embodiment 9, wherein the affinity chromatography is Protein A affinity chromatography. 11. The use of embodiment 10, wherein the Protein A affinity chromatography is selected from the group comprising MabSelectSure affinity chromatography, ProSep vA affinity chromatography, Poros Mab Capture A affinity chromatography, ProSep Ultra Plus affinity chromatography, MabSelect SuRe LX, MabSelect, Eshmuno A, Toyopearl AF-rProtein A-650F; Toyopearl AF-rProtein A HC-650HF). 12. The use of any one of the previous embodiments, wherein the host cell protein is a Chinese Hamster Ovary (CHO) host cell protein. 13. The use of embodiment 12, wherein the host cell protein is a phospholipase. 14. The use of embodiment 13, wherein the host cell protein is phospholipase A, phospholipase B, phospholipase C, or phospholipase D. 15. The use according to embodiment 12, 13 or 14, wherein the host cell protein is phospholipase B-like 2 (PLBL2). 16. The use of embodiment 12, wherein the host cell protein is phospholipase B-like 2 (PLBL2) or clusterin. 17. The use of any one of the previous embodiments, wherein the low conductivity aqueous solution contains tris(hydroxymethyl)aminomethane (tris). 18. The use of embodiment 17, wherein the low-conductivity aqueous solution comprises about 0.1 mM to about 10 mM Tris. 19. The use of embodiment 18, wherein the low-conductivity aqueous solution comprises about 0.1 mM to about 8 mM Tris. 20. The use of embodiment 19, wherein the low-conductivity aqueous solution comprises about 0.5 mM to about 6.5 mM Tris. 21. The use of embodiment 20, wherein the low conductivity aqueous solution comprises about 2 mM Tris. 22. The use of any one of embodiments 17 to 21, wherein the low-conductivity aqueous solution contains potassium phosphate. 23. The use of embodiment 22, wherein the low-conductivity aqueous solution comprises about 0.05 mM to about 5 mM potassium phosphate. 24. The use of embodiment 23, wherein the low-conductivity aqueous solution comprises about 0.05 mM to about 2 mM potassium phosphate. 25. The use of embodiment 24, wherein the low conductivity aqueous solution comprises about 0.5 mM potassium phosphate. 26. The use of any one of the previous embodiments, wherein the low-conductivity aqueous solution has a pH of about 7 or greater. 27. The use of embodiment 26, wherein the low-conductivity aqueous solution has a pH of about 7.5 or higher. 28. The use of embodiment 27, wherein the low-conductivity aqueous solution has a pH of about 7 to about 9.5. 29. The use of embodiment 28, wherein the low-conductivity aqueous solution has a pH of about 7.5 to about 8.5. 30. The use of embodiment 29, wherein the low-conductivity aqueous solution has a pH of about 8. 31. The use of any one of the previous embodiments, wherein a low conductivity aqueous solution washing step is performed after or before a high conductivity aqueous solution washing step. 32. The use of embodiment 31, wherein a low conductivity aqueous solution washing step is performed after a high conductivity aqueous solution washing step. 33. The use of embodiment 31, wherein the high conductivity aqueous solution has a conductivity value of about 20 mS / cm or more. 34. The use of embodiment 33, wherein the high conductivity aqueous solution has a conductivity value of about 20 mS / cm to about 100 mS / cm. 35. The use of embodiment 31, wherein an intermediate washing step with a medium conductivity aqueous solution is performed between the low conductivity aqueous solution washing step and the high conductivity aqueous solution washing step. 36. The use of embodiment 35, wherein the medium conductivity aqueous solution has a conductivity value of from more than 0.5 mS / cm to less than 20 mS / cm. 37. The use of any one of embodiments 33 to 36, wherein the high conductivity or medium conductivity aqueous solution comprises an amino acid. 38. The use of embodiment 37, wherein the high conductivity or medium conductivity aqueous solution comprises histidine. 39. The use of embodiment 37, wherein the high conductivity or medium conductivity aqueous solution comprises histidine and tris. 40. The use of any one of the preceding embodiments, wherein at least one additional chromatography method / step is performed. 41. The use of embodiment 40, wherein an additional ion exchange chromatography method / step is performed. 42. The use of embodiment 41, wherein an additional anion exchange chromatography method / step is performed. 43. The use of embodiment 40, wherein an additional cation exchange chromatography method / step is performed. 44. The use of embodiment 40, wherein an additional anion exchange chromatography method / step and an additional cation exchange chromatography method / step are performed. 45. The use of embodiment 40, without a hydrophobic interaction chromatography method / step. 46. ​​The use of any one of the previous embodiments, wherein the human IgG4 isotype antibody is an antibody against P-selectin, or an antibody against Factor IXa and Factor X, or an antibody against IL-13, or an antibody against amyloid beta. 47. The use of any one of embodiments 1 to 45, wherein the human IgG1 isotype antibody is an antibody against influenza B, or an antibody against VEGF-A, or an antibody against CD22, or an antibody against HER3 and EGFR, or an antibody against amyloid beta, or an antibody against Her2, or an antibody against Ang2 and VEGF-A, or an antibody against carcinoembryonic antigen (CEA) and CD3. 48. (a) culturing a cell containing a nucleic acid encoding a human IgG isotype antibody; (b) recovering human IgG isotype antibodies from the cells or culture medium; (c) contacting a human IgG isotype antibody with an affinity chromatography material; (d) washing the affinity chromatography material with a low conductivity aqueous solution; (e) recovering human IgG isotype antibodies from the affinity chromatography material. Including, thereby producing human IgG isotype antibodies, Methods for producing human IgG isotype antibodies. 49. (a) culturing a cell containing a nucleic acid encoding a human IgG4 isotype antibody; (b) recovering human IgG4 isotype antibodies from the cells or culture medium; (c) contacting a human IgG4 isotype antibody with an affinity chromatography material; (d) washing the affinity chromatography material with a low conductivity aqueous solution; (e) recovering human IgG4 isotype antibodies from the affinity chromatography material. Including, Thereby producing human IgG4 isotype antibodies. A method for producing human IgG4 isotype antibodies. 50. The method of embodiment 48, wherein the human IgG isotype antibody is a human IgG4 isotype antibody or a human IgG1 isotype antibody. 51. The method of embodiment 48, wherein the human IgG isotype antibody is a human IgG4 isotype antibody or a human IgG1 isotype antibody that has no / exactly one glycosylation site in its Fab fragment (at position Asn297 according to the Kabat numbering). 52. The method of embodiment 48, wherein the low-conductivity aqueous solution has a conductivity value of about 0.5 mS / cm or less. 53. The method of embodiment 52, wherein the low-conductivity aqueous solution has a conductivity value of about 0.03 μS / cm to about 0.5 mS / cm. 54. The method of embodiment 53, wherein the low-conductivity aqueous solution has a conductivity value of about 0.05 μS / cm to about 0.35 mS / cm. 55. The method of embodiment 54, wherein the low-conductivity aqueous solution is not deionized water. 56. The method of any one of aspects 48 to 55, wherein the affinity chromatography is Protein A affinity chromatography or Protein G affinity chromatography or single chain Fv ligand (KappaSelect) affinity chromatography. 57. The method of embodiment 56, wherein the affinity chromatography is Protein A affinity chromatography. 58. The method of embodiment 56, wherein the Protein A affinity chromatography is selected from the group comprising MabSelectSure affinity chromatography, ProSep vA affinity chromatography, Poros Mab Capture A affinity chromatography, ProSep Ultra Plus affinity chromatography, MabSelect SuRe LX, MabSelect, Eshmuno A, Toyopearl AF-rProtein A-650F; Toyopearl AF-rProtein A HC-650HF). 59. The method of embodiment 48 or 49, wherein the content of host cell proteins is reduced. 60. The method of any one of embodiments 59, wherein the host cell protein is a Chinese Hamster Ovary (CHO) host cell protein. 61. The method of embodiment 60, wherein the host cell protein is a phospholipase. 62. The method of embodiment 61, wherein the host cell protein is phospholipase A, phospholipase B, phospholipase C, or phospholipase D. 63. The method of any one of aspects of aspect 62, wherein the host cell protein is phospholipase B-like 2 (PLBL2). 64. The method of embodiment 60, wherein the host cell protein is phospholipase B-like 2 (PLBL2) or clusterin. 65. The method of any one of embodiments 48-64, wherein the low-conductivity aqueous solution contains tris(hydroxymethyl)aminomethane (tris). 66. The method of embodiment 65, wherein the low-conductivity aqueous solution comprises about 0.1 mM to about 10 mM Tris. 67. The method of embodiment 66, wherein the low-conductivity aqueous solution comprises about 0.1 mM to about 8 mM Tris. 68. The method of embodiment 67, wherein the low-conductivity aqueous solution comprises about 0.5 mM to about 6.5 mM Tris. 69. The method of embodiment 68, wherein the low conductivity aqueous solution comprises about 2 mM Tris. 70. The method of any one of embodiments 65-69, wherein the low-conductivity aqueous solution contains potassium phosphate. 71. The method of embodiment 70, wherein the low-conductivity aqueous solution comprises about 0.2 mM to about 5 mM potassium phosphate. 72. The method of embodiment 71, wherein the low-conductivity aqueous solution comprises about 0.05 mM to about 2 mM potassium phosphate. 73. The method of embodiment 72, wherein the low-conductivity aqueous solution comprises about 0.5 mM potassium phosphate. 74. The method of any one of embodiments 48-73, wherein the low-conductivity aqueous solution has a pH of about 7 or greater. 75. The method of embodiment 74, wherein the low-conductivity aqueous solution has a pH of about 7.5 or greater. 76. The method of embodiment 75, wherein the low-conductivity aqueous solution has a pH of about 7 to about 9.5. 77. The method of embodiment 76, wherein the low-conductivity aqueous solution has a pH of about 7.5 to about 8.5. 78. The method of embodiment 77, wherein the low-conductivity aqueous solution has a pH of about 8. 79. The method of embodiment 48 or 49, further comprising washing the affinity chromatography material with a high conductivity aqueous solution before or after washing the affinity chromatography material with the low conductivity aqueous solution. 80. The method of embodiment 79, further comprising washing the affinity chromatography material with a high conductivity aqueous solution before washing the affinity chromatography material with the low conductivity aqueous solution. 81. The method of embodiment 79, further comprising washing the affinity chromatography material with a high conductivity aqueous solution and / or a medium conductivity aqueous solution before or after washing the affinity chromatography material with the low conductivity aqueous solution. 82. The method of embodiment 79, further comprising washing the affinity chromatography material with a high conductivity aqueous solution and / or a medium conductivity aqueous solution before washing the affinity chromatography material with the low conductivity aqueous solution. 83. The method of any one of embodiments 79-82, wherein the high conductivity aqueous solution has a conductivity value of about 20 mS / cm or more. 84. The method of embodiment 83, wherein the high conductivity aqueous solution has a conductivity value of about 20 mS / cm to about 100 mS / cm. 85. The method of any one of embodiments 81-82, wherein the medium conductivity aqueous solution has a conductivity value from greater than 0.5 mS / cm to less than 20 mS / cm. 86. The method of any one of embodiments 79 to 85, wherein the high conductivity or medium conductivity aqueous solution comprises an amino acid. 87. The method of embodiment 86, wherein the high conductivity or medium conductivity aqueous solution comprises histidine. 88. The method of embodiment 86 or 87, wherein the high conductivity or medium conductivity aqueous solution comprises histidine and tris. 89. The method of embodiment 48 or 49, wherein at least one additional chromatography method / step is performed after step (e). 90. The method of embodiment 89, wherein an additional ion exchange chromatography method / step is performed after step (e). 91. The method of embodiment 90, wherein an additional anion exchange chromatography method / step is performed after step (e). 92. The method of embodiment 90, wherein an additional cation exchange chromatography method / step is performed after step (e). 93. The method of embodiment 90, wherein an additional anion exchange chromatography method / step and an additional cation exchange chromatography method / step are performed after step (e). 94. The method of embodiment 48 or 49, which does not involve a hydrophobic interaction chromatography method / step. 95. The method of any one of embodiments 49 to 94, wherein the human IgG4 isotype antibody is an antibody against P-selectin, or an antibody against factors IXa and X, or an antibody against IL-13, or an antibody against amyloid beta. 96. The method of any one of embodiments 48 or 50 to 94, wherein the human IgG1 isotype antibody is an antibody against influenza B, or an antibody against VEGF-A, or an antibody against CD22, or a bispecific antibody against HER3 and EGFR, or an antibody against amyloid beta, or an antibody against Her2, or a bispecific antibody against Ang2 and VEGF-A, or an antibody against carcinoembryonic antigen (CEA) and CD3. 97. (a) providing a sample containing human IgG isotype antibodies; (b) purifying human IgG isotype antibodies by an affinity chromatography method / step comprising washing the affinity chromatography material with a low conductivity aqueous solution. 1. A method for purifying human IgG isotype antibodies from a sample, comprising: 98. The method of embodiment 97, wherein the human IgG isotype antibody is a human IgG4 isotype antibody or a human IgG1 isotype antibody. 99. The method of embodiment 98, wherein the human IgG isotype antibody is a human IgG4 isotype antibody or a human IgG1 isotype antibody having no / exactly one glycosylation site in its Fab fragment (at position Asn297 according to the Kabat numbering). 100. The method of any one of embodiments 97-99, wherein the low-conductivity aqueous solution has a conductivity value of about 0.5 mS / cm or less. 101. The method of embodiment 100, wherein the low-conductivity aqueous solution has a conductivity value of about 0.03 μS / cm to about 0.5 mS / cm. 102. The method of embodiment 101, wherein the low-conductivity aqueous solution has a conductivity value of about 0.05 μS / cm to about 0.35 mS / cm. 103. The method of embodiment 102, wherein the low-conductivity aqueous solution is not deionized water. 104. The method of any one of embodiments 97-104, wherein the affinity chromatography is Protein A affinity chromatography or Protein G affinity chromatography or single chain Fv ligand (KappaSelect) affinity chromatography. 105. The method of embodiment 104, wherein the affinity chromatography is Protein A affinity chromatography. 106. The method of embodiment 105, wherein the Protein A affinity chromatography is selected from the group comprising MabSelectSure affinity chromatography, ProSep vA affinity chromatography, Poros Mab Capture A affinity chromatography, ProSep Ultra Plus affinity chromatography, MabSelect SuRe LX, MabSelect, Eshmuno A, Toyopearl AF-rProtein A-650F; Toyopearl AF-rProtein A HC-650HF). 107. The method of any one of embodiments 97 to 106, wherein the content of host cell proteins is reduced. 108. The method of embodiment 107, wherein the host cell protein is a Chinese Hamster Ovary (CHO) host cell protein. 109. The method of embodiment 108, wherein the host cell protein is a phospholipase. 110. The method of embodiment 109, wherein the host cell protein is phospholipase A, phospholipase B, phospholipase C, or phospholipase D. 111. The method of any one of aspects of aspect 110, wherein the host cell protein is phospholipase B-like 2 (PLBL2). 112. The method of embodiment 107, wherein the host cell protein is phospholipase B-like 2 (PLBL2) or clusterin. 113. The method of any one of embodiments 97-112, wherein the low-conductivity aqueous solution contains tris(hydroxymethyl)aminomethane (tris). 114. The method of embodiment 113, wherein the low-conductivity aqueous solution comprises about 0.1 mM to about 10 mM Tris. 115. The method of embodiment 114, wherein the low-conductivity aqueous solution comprises about 0.1 mM to about 8 mM Tris. 116. The method of embodiment 115, wherein the low-conductivity aqueous solution comprises about 0.5 mM to about 6.5 mM Tris. 117. The method of embodiment 116, wherein the low-conductivity aqueous solution comprises about 2 mM Tris. 118. The method of any one of embodiments 113-117, wherein the low-conductivity aqueous solution contains potassium phosphate. 119. The method of embodiment 118, wherein the low-conductivity aqueous solution comprises about 0.2 mM to about 5 mM potassium phosphate. 120. The method of embodiment 119, wherein the low-conductivity aqueous solution comprises about 0.2 mM to about 2 mM potassium phosphate. 121. The method of embodiment 120, wherein the low-conductivity aqueous solution comprises about 0.5 mM potassium phosphate. 122. The method of any one of embodiments 97-121, wherein the low-conductivity aqueous solution has a pH of about 7 or greater. 123. The method of embodiment 122, wherein the low-conductivity aqueous solution has a pH of about 7.5 or greater. 124. The method of embodiment 123, wherein the low-conductivity aqueous solution has a pH of about 7 to about 9.5. 125. The method of embodiment 124, wherein the low-conductivity aqueous solution has a pH of about 7.5 to about 8.5. 126. The method of embodiment 125, wherein the low-conductivity aqueous solution has a pH of about 8. 127. The method of any one of embodiments 97-126, further comprising washing the affinity chromatography material with a high conductivity aqueous solution before or after washing the affinity chromatography material with the low conductivity aqueous solution. 128. The method of embodiment 127, further comprising washing the affinity chromatography material with a high conductivity aqueous solution before washing the affinity chromatography material with the low conductivity aqueous solution. 129. The method of embodiment 127, further comprising washing the affinity chromatography material with a high conductivity aqueous solution and / or a medium conductivity aqueous solution before or after washing the affinity chromatography material with the low conductivity aqueous solution. 130. The method of embodiment 127, further comprising washing the affinity chromatography material with a high conductivity aqueous solution and / or a medium conductivity aqueous solution before washing the affinity chromatography material with the low conductivity aqueous solution. 131. The method of any one of embodiments 127-130, wherein the high conductivity aqueous solution has a conductivity value of about 20 mS / cm or more. 132. The method of embodiment 131, wherein the high conductivity aqueous solution has a conductivity value of about 20 mS / cm to about 100 mS / cm. 133. The method of embodiment 129 or 130, wherein the medium conductivity aqueous solution has a conductivity value of from greater than 0.5 mS / cm to less than 20 mS / cm. 134. The method of any one of embodiments 127 to 133, wherein the high conductivity aqueous solution or the medium conductivity aqueous solution comprises an amino acid. 135. The method of embodiment 134, wherein the high conductivity or medium conductivity aqueous solution comprises histidine. 136. The method of embodiment 134, wherein the high conductivity or medium conductivity aqueous solution comprises histidine and tris. 137. The method of embodiment 97 or 98, wherein at least one additional chromatography method / step is performed after step (b). 138. The method of embodiment 137, wherein an additional ion exchange chromatography method / step is performed after step (b). 139. The method of embodiment 138, wherein an additional anion exchange chromatography method / step is performed after step (b). 140. The method of embodiment 138, wherein an additional cation exchange chromatography method / step is performed after step (b). 141. The method of embodiment 138, wherein an additional anion exchange chromatography method / step and an additional cation exchange chromatography method / step are performed after step (b). 142. The method of any one of embodiments 97 to 141, which does not involve a hydrophobic interaction chromatography method / step. 143. The method of embodiment 97 or 98, wherein the human IgG4 isotype antibody is an antibody against P-selectin, or an antibody against factors IXa and X, or an antibody against IL-13, or an antibody against amyloid beta. 144. The method of embodiment 97 or 98, wherein the human IgG1 isotype antibody is an antibody against influenza B, or an antibody against VEGF-A, or an antibody against CD22, or a (bispecific) antibody against HER3 and EGFR, or an antibody against amyloid beta, or an antibody against Her2, or a bispecific antibody against Ang2 and VEGF-A, or a bispecific antibody against carcinoembryonic antigen (CEA) and CD3. 145. Use of a low conductivity aqueous solution having a conductivity value of about 0.5 mS / cm or less to reduce the content of host cell proteins in the wash step of Protein A chromatography used to purify human IgG4 isotype antibodies or human IgG1 isotype antibodies. 146. (a) culturing a cell containing a nucleic acid encoding a human IgG4 isotype antibody or a human IgG1 isotype antibody; (b) recovering human IgG4 isotype antibodies or human IgG1 isotype antibodies from the cells or culture medium; (c) contacting a human IgG4 isotype antibody or a human IgG1 isotype antibody with a Protein A chromatography material; (d) washing the Protein A chromatography material with a low-conductivity aqueous solution having a conductivity value of about 0.5 mS / cm or less; (e) recovering human IgG4 isotype antibodies or human IgG1 isotype antibodies from the Protein A chromatography material. Including, thereby producing human IgG4 isotype antibodies or human IgG1 isotype antibodies, A method for producing human IgG4 or human IgG1 isotype antibodies. 147. (a) providing a sample containing a human IgG4 isotype antibody or a human IgG1 isotype antibody; (b) purifying human IgG4 isotype antibodies or human IgG1 isotype antibodies by a Protein A chromatography method / step comprising washing the Protein A chromatography material with a low-conductivity aqueous solution having a conductivity value of about 0.5 mS / cm or less. 1. A method for purifying a human IgG4 isotype antibody or a human IgG1 isotype antibody from a sample, comprising: 148. The use of any one of embodiments 5 to 7, wherein the low conductivity aqueous solution is deionized water. 149. The method of embodiment 54, wherein the low-conductivity aqueous solution is deionized water.

[0093] Description of sequence listing SEQ ID NO:01 <vegf>The variable heavy domain VH SEQ ID NO:02 <vegf>The variable light chain domain VL SEQ ID NO:03 <ang-2>The variable heavy domain VH SEQ ID NO:04 <ang-2>The variable light chain domain VL SEQ ID NO:05 Variable heavy domain VH of anti-amyloid beta antibody (IgG1 isotype) SEQ ID NO:06 Variable light domain VL of anti-amyloid beta antibody (IgG1 isotype) SEQ ID NO:07 Anti-P-selectin antibody variable heavy chain domain VH1 SEQ ID NO:08 Anti-P-selectin antibody variable heavy chain domain VH2 SEQ ID NO:09 Anti-P-selectin antibody variable heavy chain domain VH3 SEQ ID NO:10 Variable light chain domain VL1 of anti-P-selectin antibody SEQ ID NO:11 Variable light chain domain VL2 of anti-P-selectin antibody SEQ ID NO:12 Variable light chain domain VL3 of anti-P-selectin antibody EXAMPLES

[0094] Example 1 material and method antibody The invention is illustrated using several exemplary antibodies, including: an antibody against P-selectin (anti-P-selectin antibody; inlacumab; IgG4 isotype) as described in WO 2005 / 100402 or SEQ ID NO:07 to SEQ ID NO:12; a bispecific antibody against factor IXa and factor X (anti-FIXa / X antibody; IgG4 isotype) as described in WO 2012 / 067176; an antibody against Her2; a bispecific antibody against Ang2 and VEGF-A (anti-Ang2 / VEGF-A antibody; vanucizumab; IgG1 isotype) as described in WO 2011 / 117329 or SEQ ID NO:01 to SEQ ID NO:04; an antibody against amyloid beta (anti-amyloid beta antibody; gantenerumab; IgG1 isotype) as described in WO 2003 / 070760 or SEQ ID NO:05 to SEQ ID NO:06. As described in the examples below, multiple IgG1 and IgG4 antibodies are also included herein.

[0095] Methods for detection of total host cell protein (HCP), phospholipase B-like 2 protein (PLBL2) and clusterin (a) CHO HCP assay Residual CHO HCP content in process samples is measured by electrochemiluminescence immunoassay (ECLIA) on a cobas e 411 immunoassay analyzer (Roche Diagnostics).

[0096] The assay is based on the sandwich principle using a polyclonal anti-CHO HCP antibody from sheep.

[0097] First incubation: Chinese hamster ovary host cell proteins (CHO HCPs) from 15 μL of sample (neat and / or diluted) and biotin-conjugated polyclonal CHO HCP-specific antibody form a sandwich complex that becomes bound to streptavidin-coated microparticles via biotin-streptavidin interaction.

[0098] Second incubation: After addition of a polyclonal CHO HCP-specific antibody labeled with a ruthenium complex (tris(2,2'-bipyridyl)ruthenium(II) complex), a ternary complex is formed on the microparticles.

[0099] The reaction mixture is aspirated into a measurement cell, where the microparticles are magnetically captured on the surface of an electrode. Unbound material is then removed in a washing step. Application of a voltage to the electrode then induces the emission of chemiluminescence, which is measured by a photomultiplier tube.

[0100] Finally, the concentration of CHO HCP in the test sample is calculated from a standard curve of known concentrations of CHO HCP.

[0101] (b) CHO PLBL2 assay Residual Chinese hamster ovary (CHO) phospholipase B-like 2 protein (PLBL2) content in process samples is measured by electrochemiluminescence immunoassay (ECLIA) on a cobas e 411 immunoassay analyzer (Roche Diagnostics).

[0102] The assay is based on the sandwich principle using a monoclonal anti-CHO PLBL2 antibody from mouse.

[0103] In the first incubation step, CHO PLBL2 from 30 μL of sample (neat and / or diluted), biotin-labeled monoclonal CHO PLBL2-specific antibody, and monoclonal CHO PLBL2-specific antibody labeled with a ruthenium complex (tris(2,2'-bipyridyl)ruthenium(II) complex) form a sandwich complex.

[0104] In the second step, after addition of streptavidin-coated microparticles, the ternary complex becomes bound to the solid phase via the interaction of biotin and streptavidin.

[0105] The reaction mixture is aspirated into a measurement cell, where the microparticles are magnetically captured on the surface of an electrode. Unbound material is then removed in a washing step. Application of a voltage to the electrode then induces chemiluminescence, which is measured by a photomultiplier tube.

[0106] Finally, the concentration of CHO PLBL2 in the test sample is calculated from a standard curve of CHO PLBL2 with known concentrations.

[0107] (c) Clusterin assay Residual clusterin content in process samples is measured by a commercially available assay from Merck Millipore (GyroMark HT Kit GYRCLU-37K), which was used according to the manufacturer's instructions.

[0108] Briefly, the assay involves the sequential (1) Binding of rat clusterin biotinylated capture antibody to a streptavidin-coated affinity column on a Bioaffy 1000 nL CD; (2) capture of rat clusterin molecules from the sample by anti-clusterin antibodies; (3) binding of a second dye-labeled anti-clusterin detection antibody to the captured molecules; (4) Quantification of rat clusterin using Gyrolab Evaluator It is a sandwich ELISA based on

[0109] Example 2 Purification of anti-P-selectin antibodies (IgG4 isotype) by protein A chromatography Antibody: Anti-P-selectin General Chromatography Conditions Column resin: Protein A material "Mab Select SuRe" (GE-Healthcare) Diameter: 1 cm, Height: 20.1 cm, CV: 15.79 ml Equipment: Akta Avant 150 Flow velocity: 300cm / hour at all stages

[0110] After equilibration of the Protein A affinity column (step 1), a solution containing anti-P-selectin antibody was applied to the column. Initial loading of PLBL2 measured in the solution containing anti-P-selectin antibody: 335 ng PLBL2 / mg antibody. Initial loading of clusterin measured in the solution containing anti-P-selectin antibody: 2874.8 ng clusterin / mg antibody. Initial loading of CHOP measured in the solution containing anti-P-selectin antibody: 100971 ng CHOP / mg antibody.

[0111] The chromatographic steps were carried out according to the following general scheme. Stage 1: Equilibration: Step 2: Loading of antibody-containing solution Phase 3: Cleaning I Phase 4: Cleaning II Stage 5: Cleansing III Phase 6: Wash IV (additional wash) Step 7: Elution

[0112] After elution from the Protein A affinity column, proteins were measured by size exclusion chromatography (SEC) and spectrophotometric (OD) analysis.

[0113] SEC: Resin: TSK 3000(Tosoh) Column: 300×7.8mm Flow rate: 0.5ml / min Buffer: 200 mM potassium phosphate containing 250 mM potassium chloride, adjusted to pH 7.0 Wavelength: 280nm OD: Ratio coefficient: 1.54 Wavelength: 280nm minus 320nm

[0114] Specific Buffer Conditions for Protein A Chromatography (Anti-P-Selectin Antibody) (a) Control (wash with equilibration buffer only) Step 1: Equilibration: 25 mM Tris, 25 mM NaCl, pH 7.0 Phase 2: Loading Stage 3: Wash I: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 4: Wash II: 25 mM Tris, 25 mM NaCl, pH 7.0 Stage 5: Cleaning III: --- Phase 6: Cleansing IV: --- Step 7: Elution: 50 mM acetic acid, pH 4.0

[0115] (b) Low conductivity wash (with Tris buffer only) Step 1: Equilibration: 25 mM Tris, 25 mM NaCl, pH 7.0 Phase 2: Loading Stage 3: Wash I: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 4: Wash II: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 5: Wash III: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 6: Wash IV: 2 mM Tris, pH 8.0 Step 7: Elution: 50 mM acetic acid, pH 4.0

[0116] (c) Low Conductivity Cleaning (only with potassium phosphate (KP)) Step 1: Equilibration: 25 mM Tris, 25 mM NaCl, pH 7.0 Phase 2: Loading Stage 3: Wash I: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 4: Wash II: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 5: Wash III: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 6: Wash IV: 0.5 mM potassium phosphate, pH 8.0 Step 7: Elution: 50 mM acetic acid, pH 4.0

[0117] (d) High Conductivity Wash (with Tris buffer only) Step 1: Equilibration: 25 mM Tris, 25 mM NaCl, pH 7.0 Phase 2: Loading Stage 3: Wash I: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 4: Wash II: 700 mM Tris, pH 7.2 Step 5: Wash III: 25 mM Tris, 25 mM NaCl, pH 7.0 Phase 6: Cleansing IV: --- Step 7: Elution: 50 mM acetic acid, pH 4.0

[0118] (e) Low conductivity wash (with Tris buffer only; pH ​​6.0) Step 1: Equilibration: 25 mM Tris, 25 mM NaCl, pH 7.0 Phase 2: Loading Stage 3: Wash I: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 4: Wash II: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 5: Wash III: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 6: Wash IV: 2 mM Tris, pH 6.0 Step 7: Elution: 50 mM acetic acid, pH 4.0

[0119] (f) High conductivity wash (with histidine (His) / Tris buffer only) Step 1: Equilibration: 25 mM Tris, 25 mM NaCl, pH 7.0 Phase 2: Loading Stage 3: Wash I: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 4: Wash II: 200mM His / 1000mM Tris, pH 7.0 Step 5: Wash III: 25 mM Tris, 25 mM NaCl, pH 7.0 Phase 6: Cleansing IV: --- Step 7: Elution: 50 mM acetic acid, pH 4.0

[0120] (g) Low conductivity Tris + high conductivity His / Tris Step 1: Equilibration: 25 mM Tris, 25 mM NaCl, pH 7.0 Phase 2: Loading Stage 3: Wash I: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 4: Wash II: 200mM His / 1000mM Tris, pH 7.0 Step 5: Wash III: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 6: Wash IV: 2 mM Tris, pH 8.0 Step 7: Elution: 50 mM acetic acid, pH 4.0

[0121] (h) Low conductivity potassium phosphate (KP) + high conductivity histidine (His) / Tris Step 1: Equilibration: 25 mM Tris, 25 mM NaCl, pH 7.0 Phase 2: Loading Stage 3: Wash I: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 4: Wash II: 200mM His / 1000mM Tris, pH 7.0 Step 5: Wash III: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 6: Wash IV: 0.5 mM potassium phosphate, pH 8.0 Step 7: Elution: 50 mM acetic acid, pH 4.0

[0122] (i) Low conductivity TRIS + high conductivity TRIS Step 1: Equilibration: 25 mM Tris, 25 mM NaCl, pH 7.0 Phase 2: Loading Stage 3: Wash I: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 4: Wash II: 700 mM Tris, pH 7.2 Step 5: Wash III: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 6: Wash IV: 2 mM Tris, pH 8.0 Step 7: Elution: 50 mM acetic acid, pH 4.0

[0123] (j) Low conductivity Tris; pH 6.0+ high conductivity Tris Step 1: Equilibration: 25 mM Tris, 25 mM NaCl, pH 7.0 Phase 2: Loading Stage 3: Wash I: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 4: Wash II: 700 mM Tris, pH 7.2 Step 5: Wash III: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 6: Wash IV: 2 mM Tris, pH 6.0 Step 7: Elution: 50 mM acetic acid, pH 4.0

[0124] result: TIFF2025031829000001.tif125147

[0125] Example 3 Purification of anti-amyloid beta antibodies (IgG1 isotype) by protein A chromatography The general conditions were as described in Example 2. Antibody: anti-amyloid beta. Initial loading of PLBL2 measured in a solution containing anti-amyloid beta antibodies: 2019.7ng PLBL2 / mg antibody. Initial loading of CHOP measured in a solution containing anti-amyloid beta antibodies: 578908ng CHOP / mg antibody.

[0126] Specific buffer conditions for Protein A chromatography (a) Control (wash with equilibration buffer only) Step 1: Equilibration: 25 mM Tris, 25 mM NaCl, pH 7.0 Phase 2: Loading Stage 3: Wash I: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 4: Wash II: 25 mM Tris, 25 mM NaCl, pH 7.0 Stage 5: Cleaning III: --- Phase 6: Cleansing IV: --- Step 7: Elution: 50 mM acetic acid, pH 4.0

[0127] (b) Low conductivity wash (with Tris buffer only) Step 1: Equilibration: 25 mM Tris, 25 mM NaCl, pH 7.0 Phase 2: Loading Stage 3: Wash I: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 4: Wash II: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 5: Wash III: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 6: Wash IV: 2 mM Tris, pH 8.0 Step 7: Elution: 50 mM acetic acid, pH 4.0

[0128] (c) High conductivity wash (with Tris buffer only) Step 1: Equilibration: 25 mM Tris, 25 mM NaCl, pH 7.0 Phase 2: Loading Stage 3: Wash I: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 4: Wash II: 700 mM Tris, pH 7.2 Step 5: Wash III: 25 mM Tris, 25 mM NaCl, pH 7.0 Phase 6: Cleansing IV: --- Step 7: Elution: 50 mM acetic acid, pH 4.0

[0129] (d) Low conductivity Tris + high conductivity His / Tris Step 1: Equilibration: 25 mM Tris, 25 mM NaCl, pH 7.0 Phase 2: Loading Stage 3: Wash I: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 4: Wash II: 200mM His / 1000mM Tris, pH 7.0 Step 5: Wash III: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 6: Wash IV: 2 mM Tris, pH 8.0 Step 7: Elution 50 mM acetic acid, pH 4.0

[0130] TIFF2025031829000002.tif61147

[0131] Example 4 Purification of anti-Her2 antibody (IgG1 isotype) by protein A chromatography The general conditions were as described in Example 2. Antibody: Anti-Her2 Initial loading of PLBL2 measured in a solution containing anti-Her2 antibody: 1662.5 ng PLBL2 / mg antibody. Initial loading of CHOP measured in a solution containing anti-Her2 antibody: 727070 ng CHOP / mg antibody.

[0132] Specific buffer conditions for Protein A chromatography (a) Control (wash with equilibration buffer only) Step 1: Equilibration: 25 mM Tris, 25 mM NaCl, pH 7.0 Phase 2: Loading Stage 3: Wash I: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 4: Wash II: 25 mM Tris, 25 mM NaCl, pH 7.0 Stage 5: Cleaning III: --- Phase 6: Cleansing IV: --- Step 7: Elution: 50 mM acetic acid, pH 4.0

[0133] (b) Low conductivity wash (with Tris buffer only) Step 1: Equilibration: 25 mM Tris, 25 mM NaCl, pH 7.0 Phase 2: Loading Stage 3: Wash I: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 4: Wash II: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 5: Wash III: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 6: Wash IV: 2 mM Tris, pH 8.0 Step 7: Elution: 50 mM acetic acid, pH 4.0

[0134] (c) High conductivity wash (with Tris buffer only) Step 1: Equilibration: 25 mM Tris, 25 mM NaCl, pH 7.0 Phase 2: Loading Stage 3: Wash I: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 4: Wash II: 700 mM Tris, pH 7.2 Step 5: Wash III: 25 mM Tris, 25 mM NaCl, pH 7.0 Phase 6: Cleansing IV: --- Step 7: Elution: 50 mM acetic acid, pH 4.0

[0135] (d) Low conductivity Tris + high conductivity His / Tris Step 1: Equilibration: 25 mM Tris, 25 mM NaCl, pH 7.0 Phase 2: Loading Stage 3: Wash I: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 4: Wash II: 200mM His / 1000mM Tris, pH 7.0 Step 5: Wash III: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 6: Wash IV: 2 mM Tris, pH 8.0 Step 7: Elution: 50 mM acetic acid, pH 4.0

[0136] TIFF2025031829000003.tif61147

[0137] Example 5 Purification of a bispecific anti-Ang2 / VEGF-A antibody (IgG1 isotype) by protein A chromatography The general conditions were as described in Example 2. Antibody: Anti-Ang2 / VEGF-A Initial loading of PLBL2 measured in a solution containing bispecific anti-Ang2 / VEGF-A antibody: 919.7 ng PLBL2 / mg antibody. Initial loading of CHOP measured in a solution containing anti-Ang2 / VEGF-A: 682304 ng CHOP / mg antibody.

[0138] Specific buffer conditions for Protein A chromatography (a) Control (wash with equilibration buffer only) Step 1: Equilibration: 25 mM Tris, 25 mM NaCl, pH 7.0 Phase 2: Loading Stage 3: Wash I: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 4: Wash II: 25 mM Tris, 25 mM NaCl, pH 7.0 Stage 5: Cleaning III: --- Phase 6: Cleansing IV: --- Step 7: Elution: 50 mM acetic acid, pH 4.0

[0139] (b) Low conductivity wash (with Tris buffer only) Step 1: Equilibration: 25 mM Tris, 25 mM NaCl, pH 7.0 Phase 2: Loading Stage 3: Wash I: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 4: Wash II: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 5: Wash III: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 6: Wash IV: 2 mM Tris, pH 8.0 Step 7: Elution: 50 mM acetic acid, pH 4.0

[0140] (c) High conductivity wash (with Tris buffer only) Step 1: Equilibration: 25 mM Tris, 25 mM NaCl, pH 7.0 Phase 2: Loading Stage 3: Wash I: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 4: Wash II: 700 mM Tris, pH 7.2 Step 5: Wash III: 25 mM Tris, 25 mM NaCl, pH 7.0 Phase 6: Cleansing IV: --- Step 7: Elution: 50 mM acetic acid, pH 4.0

[0141] (d) Low conductivity Tris + high conductivity His / Tris Step 1: Equilibration: 25 mM Tris, 25 mM NaCl, pH 7.0 Phase 2: Loading Stage 3: Wash I: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 4: Wash II: 200mM His / 1000mM Tris, pH 7.0 Step 5: Wash III: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 6: Wash IV: 2 mM Tris, pH 8.0 Step 7: Elution: 50 mM acetic acid, pH 4.0

[0142] TIFF2025031829000004.tif61147

[0143] Example 6 Purification of bispecific anti-FIXa / X antibody (IgG4 isotype) by protein A chromatography The purification of anti-FIXa / X antibodies was tested in two different chromatographic setups.

[0144] Setup 1 The general conditions were as described in Example 2. Antibody: Anti-FIXa / X Initial loading of PLBL2 measured in a solution containing anti-FIXa / X antibody: 557ng PLBL2 / mg antibody. Initial loading of CHOP measured in a solution containing anti-FIXa / X: 387377ng CHOP / mg antibody.

[0145] Specific buffer conditions for Protein A chromatography (a) High conductivity wash (with Tris buffer only) Step 1: Equilibration: 25 mM Tris, 25 mM NaCl, pH 7.0 Phase 2: Loading Stage 3: Wash I: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 4: Wash II: 700 mM Tris, pH 7.2 Step 5: Wash III: 25 mM Tris, 25 mM NaCl, pH 7.0 Phase 6: Cleansing IV: --- Step 7: Elution: 50 mM acetic acid, pH 4.0

[0146] (b) Low conductivity Tris + high conductivity His / Tris Step 1: Equilibration: 25 mM Tris, 25 mM NaCl, pH 7.0 Phase 2: Loading Stage 3: Wash I: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 4: Wash II: 200mM His / 1000mM Tris, pH 7.0 Step 5: Wash III: 25 mM Tris, 25 mM NaCl, pH 7.0 Step 6: Wash IV: 2 mM Tris, pH 8.0 Step 7: Elution: 50 mM acetic acid, pH 4.0

[0147] TIFF2025031829000005.tif39147

[0148] Configuration 2 General Chromatography Conditions Column resin: Protein A material "Mab Select SuRe" (GE-Healthcare) Diameter: 1 cm, Height: 20.1 cm, CV: 15.79 ml Equipment: Akta Avant 150 Flow velocity: 300cm / hour at all stages

[0149] After equilibration of the Protein A affinity column (step 1), a solution containing anti-FIXa / X antibodies was applied to the column.

[0150] Initial loading of PLBL2 measured in a solution containing anti-FIXa / X antibody: 557 ng PLBL2 / mg antibody.

[0151] The chromatographic steps were carried out according to the following general scheme. Stage 1: Equilibration: Step 2: Loading of antibody-containing solution Phase 3: Cleaning I Phase 4: Cleaning II Phase 5: Wash III (additional wash) Step 6: Elution

[0152] Specific buffer conditions for Protein A chromatography (a) High Conductivity Wash (NaSO4 buffer only) Step 1: Equilibration: 20mM NaPO4, pH 7.5 Phase 2: Loading Stage 3: Wash I: 450 mM NaSO4, 20 mM NaAc, pH 4.8 Stage 4: Wash II: 20 mM NaPO4, pH 7.5 Stage 5: Cleaning III: --- Step 6: Elution: 35 mM acetic acid, pH 4.0

[0153] (b) Low conductivity wash (Tris 1 mM) + high conductivity wash (with NaSO4) Step 1: Equilibration: 20mM NaPO4, pH 7.5 Phase 2: Loading Stage 3: Wash I: 450 mM NaSO4, 20 mM NaAc, pH 4.8 Stage 4: Wash II: 20 mM NaPO4, pH 7.5 Step 5: Wash III: 1 mM Tris, pH 8.0 Step 6: Elution: 50 mM acetic acid, pH 4.0

[0154] (c) Low conductivity wash (Tris 2 mM) + high conductivity wash (with NaSO4) Step 1: Equilibration: 20mM NaPO4, pH 7.5 Phase 2: Loading Stage 3: Wash I: 450 mM NaSO4, 20 mM NaAc, pH 4.8 Stage 4: Wash II: 20 mM NaPO4, pH 7.5 Step 5: Wash III: 2 mM Tris, pH 8.0 Step 6: Elution: 35 mM acetic acid, pH 4.0

[0155] (d) Low conductivity wash (Tris 4 mM) + high conductivity wash (with NaSO4) Step 1: Equilibration: 20mM NaPO4, pH 7.5 Phase 2: Loading Stage 3: Wash I: 450 mM NaSO4, 20 mM NaAc, pH 4.8 Stage 4: Wash II: 20 mM NaPO4, pH 7.5 Step 5: Wash III: 4 mM Tris, pH 8.0 Step 6: Elution: 50 mM acetic acid, pH 4.0

[0156] (e) Low conductivity wash (Tris 6 mM) + high conductivity wash (with NaSO4) Step 1: Equilibration: 20mM NaPO4, pH 7.5 Phase 2: Loading Stage 3: Wash I: 450 mM NaSO4, 20 mM NaAc, pH 4.8 Stage 4: Wash II: 20 mM NaPO4, pH 7.5 Step 5: Wash III: 6 mM Tris, pH 8.0 Step 6: Elution: 50 mM acetic acid, pH 4.0

[0157] (f) Low conductivity wash (Tris 4 mM, pH 7.8) + high conductivity wash (with NaSO4) Step 1: Equilibration: 20mM NaPO4, pH 7.5 Phase 2: Loading Stage 3: Wash I: 450 mM NaSO4, 20 mM NaAc, pH 4.8 Stage 4: Wash II: 20 mM NaPO4, pH 7.5 Step 5: Wash III: 4 mM Tris, pH 7.8 Step 6: Elution: 50 mM acetic acid, pH 4.0

[0158] (g) Low conductivity wash (Tris 4 mM, pH 8.2) + high conductivity wash (with NaSO4) Step 1: Equilibration: 20mM NaPO4, pH 7.5 Phase 2: Loading Stage 3: Wash I: 450 mM NaSO4, 20 mM NaAc, pH 4.8 Stage 4: Wash II: 20 mM NaPO4, pH 7.5 Step 5: Wash III: 4 mM Tris, pH 8.2 Step 6: Elution: 50 mM acetic acid, pH 4.0

[0159] (h) Low conductivity wash (Tris 2 mM) + high conductivity wash (with histidine (His) / Tris 1M) Step 1: Equilibration: 20mM NaPO4, pH 7.5 Phase 2: Loading Step 3: Wash I: 200mM His / 1000mM Tris, pH 7.0 Stage 4: Wash II: 20 mM NaPO4, pH 7.5 Step 5: Wash III: 2 mM Tris, pH 8.0 Step 6: Elution: 35 mM acetic acid, pH 4.0

[0160] (i) Low conductivity wash (Tris 2 mM) + high conductivity wash (histidine (His) / Tris 0.85M) Step 1: Equilibration: 20mM NaPO4, pH 7.5 Phase 2: Loading Step 3: Wash I: 200 mM His / 850 mM Tris, pH 7.0 Stage 4: Wash II: 20 mM NaPO4, pH 7.5 Step 5: Wash III: 2 mM Tris, pH 8.0 Step 6: Elution: 50 mM acetic acid, pH 4.0

[0161] (j) Low conductivity wash (Tris 2 mM) + high conductivity wash (histidine (His) / Tris 0.7M) Step 1: Equilibration: 20mM NaPO4, pH 7.5 Phase 2: Loading Step 3: Wash I: 200mM His / 700mM Tris, pH 7.0 Stage 4: Wash II: 20 mM NaPO4, pH 7.5 Step 5: Wash III: 2 mM Tris, pH 8.0 Step 6: Elution: 50 mM acetic acid, pH 4.0

[0162] (k) Low conductivity wash (Tris 2 mM) + high conductivity wash (histidine (His) / Tris 0.55M) Step 1: Equilibration: 20mM NaPO4, pH 7.5 Phase 2: Loading Step 3: Wash I: 200 mM His / 550 mM Tris, pH 7.0 Stage 4: Wash II: 20 mM NaPO4, pH 7.5 Step 5: Wash III: 2 mM Tris, pH 8.0 Step 6: Elution: 50 mM acetic acid, pH 4.0

[0163] TIFF2025031829000006.tif136147

[0164] Example 7 General Procedures / Conditions: Mock cell culture medium Null harvested cell culture fluid was produced using non-transfected CHO-DP12 cells grown in serum-free medium. Fermentation was performed at 2 L scale using a representative cell culture process. At the end of 14 days of fermentation, the cell culture fluid was harvested by centrifugation and sterile filtration. This harvested cell culture fluid (HCCF) was then stored at -70°C until further experimentation.

[0165] Purified PLBL2 Recombinant CHO PLBL2 with a C-terminal hexahistidine tag was expressed by transient transfection at a 35 L scale and purified from harvested cell culture fluid as previously described (Vanderlaan et al., 2015). Purified PLBL2 was then formulated in PBS and stored at -70 °C until further study.

[0166] purified antibody Recombinant humanized antibodies were expressed in CHO cells and purified using column chromatography to ensure that the PLBL2 concentration was less than 20 ng / mg. Prior to the start of each study, each antibody was buffer exchanged into PBS using a PD-10 desalting column (GE Healthcare).

[0167] Preparation of loading material for Protein A chromatography To standardize the populations and abundance of host cell proteins in the Protein A load between antibodies, purified antibodies were diluted to the same concentration in PBS and spiked into HCCF from non-producing cell lines to give a final antibody titer of 5 g / L. Controls were also prepared in which PBS was added in place of purified antibody to assess non-specific host cell protein binding to the Protein A resin in the absence of antibody.

[0168] Packed Bed Column Chromatography All packed bed column chromatography experiments were performed using a MabSelect SuRe (GE Healthcare) Protein A resin column with 0.66 cm internal diameter × 20 cm bed height. For each purification, the column was first equilibrated with 25 mM Tris, 25 mM NaCl, pH 7.7 (equilibration buffer) for 3 column volumes (CV). Protein A load was then applied to a target loading density of 30 g antibody / L resin, after which the column was washed with 3 CV of equilibration buffer, 3 CV of different types of wash buffer, and again with 3 CV of equilibration buffer. The antibody was then eluted at low pH using 0.1–0.15 M acetic acid, and collection of eluate pools was started from 0.5 OD at the beginning of the elution peak and pooling was stopped after 2.8 CV. For the control experiment with PBS-spiked null HCCF, a mock eluate pool of 2.8 CV was obtained starting 1 CV after the beginning of the elution phase until 3.8 CV. At the end of each run, each Protein A eluate was then titrated to pH 5.0 using 1.5 M Tris base. The column was then sanitized with 0.1 M sodium hydroxide solution. The volumetric flow rate was 20 CV / h for all phases except for the loading phase, the first equilibration wash phase, and the elution phase, where the flow rate was 15 CV / h.

[0169] (A) Purification of an exemplary antibody (IgG4 isotype), Antibody A, by Protein A chromatography. Specific wash buffer conditions for purifying ANTIBODY A (IgG4 isotype) using the general procedure of Example 7 (outlined above): (a) 0.4 M potassium phosphate, pH 7.0 (b) 25 mM Tris, 25 mM NaCl, pH 7.7 (c) 0.75M Arg-HCl, pH 7.0 (d) 0.6 M NaCl, pH 7.0 (e) Deionized water

[0170] result: TIFF2025031829000007.tif89128

[0171] (B) Purification of an exemplary antibody (IgG1 isotype), Antibody B, by Protein A chromatography. Specific wash buffer conditions for purifying Antibody B (IgG1 isotype) using the general procedure in Example 7: (a) Deionized water

[0172] result: TIFF2025031829000008.tif46128

[0173] (C) Purification of an exemplary antibody (IgG4 isotype), Antibody C, by Protein A chromatography. Specific wash buffer conditions for purifying Antibody C (IgG4 isotype) using the general procedure in Example 7: (a) 0.4 M potassium phosphate, pH 7.0 (b) 25 mM Tris, 25 mM NaCl, pH 7.7 (c) 0.75M Arg-HCl, pH 7.0 (d) 0.6 M NaCl, pH 7.0 (e) Deionized water

[0174] result: TIFF2025031829000009.tif89128

[0175] (D) Purification of an exemplary antibody (IgG1 isotype), Antibody D, by Protein A chromatography. Specific wash buffer conditions for purifying Antibody D (IgG1 isotype) using the general procedure in Example 7: (a) Deionized water

[0176] TIFF2025031829000010.tif46128

[0177] (E) Purification of an exemplary antibody (IgG1 isotype), Antibody E, by Protein A chromatography. Specific wash buffer conditions for purifying Antibody E (IgG1 isotype) using the general procedure in Example 7: (a) 0.4 M potassium phosphate, pH 7.0 (b) 31 mM Tris, pH 8.5 (c) 55 mM Tris, pH 9.0 (d) Deionized water

[0178] result: TIFF2025031829000011.tif72128

[0179] (F) Purification of an exemplary antibody (IgG1 isotype), Antibody F, by Protein A chromatography. Specific wash buffer conditions for purifying Antibody F using the general procedure in Example 7: (a) 25 mM Tris, pH 9.0

[0180] result: TIFF2025031829000012.tif39128

[0181] Sequence information SEQUENCE LISTING <110> F. Hoffmann-La Roche AG Genentech, Inc. <120> Method for the reduction of host cell proteins in affinity Chromatography <150> US 62 / 208,523 <151> 2015-08-21 <160> 12 <170> PatentIn version 3.5 <210> 1 <211> one two three <212> PRT <213> Artificial <220> <223> variable heavy chain domain VH of <vegf>bevacizumab <400> 1 Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 1 5 10 15 Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 20 25 30 Gly Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 35 40 45 Gly Trp Ile Asn Thr Tyr Thr Gly Glu Pro Thr Tyr Ala Ala Asp Phe 50 55 60 Lys Arg Arg Phe Thr Phe Ser Leu Asp Thr Ser Lys Ser Thr Ala Tyr 65 70 75 80 Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95 Ala Lys Tyr Pro His Tyr Tyr Gly Ser Ser His Trp Tyr Phe Asp Val 100 105 110 Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 115 120 <210> 2 <211> 107 <212> PRT <213> Artificial <220> <223> variable light chain domain VL of <vegf>bevacizumab <400> 2 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Ser Ala Ser Gln Asp Ile Ser Asn Tyr 20 25 30 Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Val Leu Ile 35 40 45 Tyr Phe Thr Ser Ser Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 50 55 60 Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 65 70 75 80 Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Ser Thr Val Pro Trp 85 90 95 Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 100 105 <210> 3 <211> 128 <212> PRT <213> Artificial <220> <223> variable heavy chain domain VH of <ang-2>E6Q <400> 3 Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 1 5 10 15 Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Gly Tyr 20 25 30 Tyr Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 35 40 45 Gly Trp Ile Asn Pro Asn Ser Gly Gly Thr Asn Tyr Ala Gln Lys Phe 50 55 60 Gln Gly Arg Val Thr Met Thr Arg Asp Thr Ser Ile Ser Thr Ala Tyr 65 70 75 80 Met Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys 85 90 95 Ala Arg Ser Pro Asn Pro Tyr Tyr Tyr Asp Ser Ser Gly Tyr Tyr Tyr 100 105 110 Pro Gly Ala Phe Asp Ile Trp Gly Gln Gly Thr Met Val Thr Val Ser 115 120 125 <210> 4 <211> 108 <212> PRT <213> Artificial <220> <223> variable light chain domain VL of < ANG-2> E6Q <400> 4 Gln Pro Gly Leu Thr Gln Pro Pro Ser Val Ser Val Ala Pro Gly Gln 1 5 10 15 Thr Ala Arg Ile Thr Cys Gly Gly Asn Asn Ile Gly Ser Lys Ser Val 20 25 30 His Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Val Leu Val Val Tyr 35 40 45 Asp Asp Ser Asp Arg Pro Ser Gly Ile Pro Glu Arg Phe Ser Gly Ser 50 55 60 Asn Ser Gly Asn Thr Ala Thr Leu Thr Ile Ser Arg Val Glu Ala Gly 65 70 75 80 Asp Glu Ala Asp Tyr Tyr Cys Gln Val Trp Asp Ser Ser Ser Asp His 85 90 95 Tyr Val Phe Gly Thr Gly Thr Lys Val Thr Val Leu 100 105 <210> 5 <211> 126 <212> PRT <213> Homo sapiens <400> 5 Gln Val Glu Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 1 5 10 15 Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 20 25 30 Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 35 40 45 Ser Ala Ile Asn Ala Ser Gly Thr Arg Thr Tyr Tyr Ala Asp Ser Val 50 55 60 Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 65 70 75 80 Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95 Ala Arg Gly Lys Gly Asn Thr His Lys Pro Tyr Gly Tyr Val Arg Tyr 100 105 110 Phe Asp Val Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 115 120 125 <210> 6 <211> 108 <212> PRT <213> Homo sapiens <400> 6 Asp Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly 1 5 10 15 Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Ser Ser Ser 20 25 30 Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu 35 40 45 Ile Tyr Gly Ala Ser Ser Arg Ala Thr Gly Val Pro Ala Arg Phe Ser 50 55 60 Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Glu 65 70 75 80 Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Leu Gln Ile Tyr Asn Met Pro 85 90 95 Ile Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 100 105 <210> 7 <211> 124 <212> PRT <213> Homo sapiens <400> 7 Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 1 5 10 15 Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 20 25 30 Asp Met His Trp Val Arg Gln Ala Thr Gly Lys Gly Leu Glu Trp Val 35 40 45 Ser Gly Ile Thr Thr Ala Gly Asp Thr Tyr Tyr Pro Gly Ser Val Lys 50 55 60 Gly Arg Phe Thr Ile Ser Arg Glu Asn Ala Lys Asn Ser Leu Tyr Leu 65 70 75 80 Gln Met Asn Ser Leu Arg Ala Gly Asp Thr Ala Val Tyr Tyr Cys Ala 85 90 95 Arg Gly Arg Ile Ser Met Asp Arg Gly Val Lys Asn Asn Trp Phe Asp 100 105 110 Pro Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 115 120 <210> 8 <211> 124 <212> PRT <213> Homo sapiens <400> 8 Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Arg Pro Gly Gly 1 5 10 15 Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asn Tyr 20 25 30 Asp Met His Trp Val Arg Gln Ala Thr Gly Lys Gly Leu Glu Trp Val 35 40 45 Ser Ala Ile Thr Ala Ala Gly Asp Ile Tyr Tyr Pro Gly Ser Val Lys 50 55 60 Gly Arg Phe Thr Ile Ser Arg Glu Asn Ala Lys Asn Ser Leu Tyr Leu 65 70 75 80 Gln Met Asn Ser Leu Arg Ala Gly Asp Thr Ala Val Tyr Tyr Cys Ala 85 90 95 Arg Gly Arg Tyr Ser Gly Ser Gly Ser Tyr Tyr Asn Asp Trp Phe Asp 100 105 110 Pro Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 115 120 <210> 9 <211> 124 <212> PRT <213> Homo sapiens <400> 9 Gln Pro Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 1 5 10 15 Ser Val Lys Val Ser Cys Lys Val Ser Gly Asn Thr Leu Thr Glu Leu 20 25 30 Ser Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Met 35 40 45 Gly Gly Phe Asp Pro Glu Asn Gly Glu Ala Ile Tyr Ala Gln Lys Phe 50 55 60 Gln Gly Arg Val Thr Met Thr Ala Asp Thr Ser Thr Asp Thr Ala Tyr 65 70 75 80 Met Asp Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95 Ala Thr Asp Leu Ala Gly Gly Ser Asp Phe Tyr Tyr Tyr Gly Leu Asp 100 105 110 Val Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 115 120 <210> 10 <211> 107 <212> PRT <213> Homo sapiens <400> 10 Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly 1 5 10 15 Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Ser Ser Tyr 20 25 30 Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile 35 40 45 Tyr Asp Ala Ser Asn Arg Ala Thr Gly Ile Pro Ala Arg Phe Ser Gly 50 55 60 Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Glu Pro 65 70 75 80 Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Arg Asn Asn Trp Pro Leu 85 90 95 Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 100 105 <210> 11 <211> 107 <212> PRT <213> Homo sapiens <400> 11 Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly 1 5 10 15 Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Ser Ser Tyr 20 25 30 Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile 35 40 45 Tyr Asp Ala Ser Asn Arg Ala Thr Gly Ile Pro Ala Arg Phe Ser Gly 50 55 60 Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Glu Pro 65 70 75 80 Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Arg Ser Asn Trp Pro Leu 85 90 95 Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 100 105 <210> 12 <211> 107 <212> PRT <213> Homo sapiens <400> 12 Ala Ile Gln Leu Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Gly Ile Ser Ser Ala 20 25 30 Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 35 40 45 Tyr Asp Ala Ser Ser Leu Glu Ser Gly Val Pro Ser Arg Phe Ser Gly 50 55 60 Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 65 70 75 80 Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Phe Asn Ser Tyr Pro Tyr 85 90 95 Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 100 105 < / vegf> < / vegf> < / vegf> < / vegf>

Claims

1. 1. Use of a high conductivity aqueous solution having a conductivity value of 20 mS / cm or greater to reduce the content of host cell proteins in the wash step of Protein A chromatography used to purify human IgG1 isotype antibodies, wherein the host cell protein is phospholipase B-like 2 (PLBL2).

2. 2. The use of claim 1, wherein a low-conductivity aqueous solution washing step is performed after or before a high-conductivity aqueous solution washing step, the low-conductivity aqueous solution having a conductivity value of 0.5 mS / cm or less.

3. 3. The use according to claim 1 or 2, wherein the high conductivity aqueous solution comprises histidine.

4. 4. The use according to any one of claims 1 to 3, wherein the human IgG1 isotype antibody is an antibody against influenza B, or an antibody against VEGF-A, or an antibody against CD22, or a bispecific antibody against HER3 and EGFR, or an antibody against amyloid beta, or an antibody against Her2, or a bispecific antibody against Ang2 and VEGF-A, or a bispecific antibody against carcinoembryonic antigen (CEA) and CD3.

5. (a) culturing cells containing nucleic acids encoding human IgG1 isotype antibodies; (b) recovering human IgG1 isotype antibodies from the cells or culture medium; (c) contacting a human IgG1 isotype antibody with a Protein A chromatography material; (d) washing the Protein A chromatography material with a high conductivity aqueous solution having a conductivity value of 20 mS / cm or greater, wherein the amount of host cell protein is reduced, and the host cell protein is phospholipase B-like 2 (PLBL2); (e) recovering human IgG1 isotype antibodies from the Protein A chromatography material Including, thereby producing human IgG1 isotype antibodies, A method for producing human IgG1 isotype antibodies.

6. (a) providing a sample containing human IgG1 isotype antibodies, the sample containing host cell proteins; (b) purifying a human IgG1 isotype antibody by a Protein A chromatography method / step comprising washing the Protein A chromatography material with a high conductivity aqueous solution having a conductivity value of 20 mS / cm or greater, wherein the amount of host cell protein is reduced, and the host cell protein is phospholipase B-like 2 (PLBL2). A method for purifying human IgG1 isotype antibodies from a sample, comprising:

7. further comprising washing the affinity chromatography material with a low conductivity aqueous solution and / or a medium conductivity aqueous solution before or after washing the Protein A chromatography material with a high conductivity aqueous solution; the low-conductivity aqueous solution has a conductivity value of 0.5 mS / cm or less; The medium conductivity aqueous solution has a conductivity value of from greater than 0.5 mS / cm to less than 20 mS / cm.

7. The method of claim 5 or 6.

8. 8. The method of claim 7, wherein the high conductivity or medium conductivity aqueous solution comprises histidine.

9. 9. The method of any one of claims 5 to 8, wherein the human IgG1 isotype antibody is an antibody against influenza B, or an antibody against VEGF-A, or an antibody against CD22, or a bispecific antibody against HER3 and EGFR, or an antibody against amyloid beta, or an antibody against Her2, or a bispecific antibody against Ang2 and VEGF-A, or a bispecific antibody against carcinoembryonic antigen (CEA) and CD3.