Method for producing target protein

JP2025039879A5Active Publication Date: 2025-10-24NAGASE VIITA CO LTD
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Patent Information

Application Number
JP2025006726
Authority / Receiving Office
JP · JP
Patent Type
Applications
Current Assignee / Owner
Filing Date
2025-01-17
Publication Date
2025-10-24
Estimated Expiration
2040-06-12

AI Technical Summary

Technical Problem

Existing methods for producing target proteins using vectors in host cells often require antibiotic resistance genes, which can lead to contamination and environmental concerns, and typically involve special genetic manipulation or specific sequences that are not always necessary.

Method used

A method for producing target proteins that involves culturing cells transformed with a vector that includes a gene for the target protein but does not include an antibiotic resistance gene, a recognition sequence of a recombinase, and a gene essential for cell survival, such as a ribosomal recycling factor or a translation initiation factor.

Benefits of technology

This method allows for stable maintenance of the vector without special genetic manipulation on the host cell and without using antibiotic resistance genes, thereby eliminating the risk of contamination and environmental impact.

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Abstract

To provide a method for producing target protein while stably maintaining a vector without performing special genetic engineering to a host cell and without using a drug-resistant gene or the like.SOLUTION: A method for producing target protein including a process of culturing a cell transformed by a vector, in which the vector includes a gene of target protein, and does not include an antibiotic-resistant gene, a recognition sequence of recombinase, and a gene necessary for survival of a cell.SELECTED DRAWING: None
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