Multispecific antigen-binding molecules having blood coagulation factor viii (FVIII) cofactor function-substituting activity and pharmaceutical formulations containing such molecule as active ingredient

Abstract

To provide multispecific antigen-binding molecules having blood coagulation factor VIII (FVIII) cofactor function-substituting activity and pharmaceutical formulations thereof.SOLUTION: A bispecific antibody has a first antigen-binding site which binds to blood coagulation factor IX (FIX) and / or activated blood coagulation factor IX, and a second antigen-binding site which binds to blood coagulation factor X, where, the FIX activation-inhibiting activity is not elevated and the FVIII cofactor function-substituting activity is elevated. There are also provided pharmaceutical formulations thereof.SELECTED DRAWING: None

Description

[Technical field]

[0001] The present invention relates to a multispecific antigen-binding molecule having a blood coagulation factor VIII (FVIII) cofactor functional substituting activity and a pharmaceutical preparation thereof, and also to an antigen-binding molecule in which the association between the heavy chain and the light chain is controlled, a method for producing an antigen-binding molecule in which the association between the heavy chain and the light chain is controlled, and a method for controlling the association between the heavy chain and the light chain of an antigen-binding molecule. [Background technology]

[0002] Hemophilia A is a bleeding disorder caused by a congenital hypofunction or deficiency of blood coagulation factor VIII (FVIII). For bleeding in patients with hemophilia A, FVIII preparations are usually administered (on-demand administration). In recent years, FVIII preparations have been administered prophylactically to prevent bleeding events (Non-Patent Documents 1 and 2) (prophylactic administration). The half-life of FVIII preparations in blood is about 12 to 16 hours. Therefore, for continuous prevention, FVIII preparations are administered to patients three times a week (Non-Patent Documents 3 and 4). In on-demand administration, FVIII preparations are additionally administered at regular intervals as necessary to prevent rebleeding. Furthermore, FVIII preparations are administered intravenously. Therefore, there has been a strong demand for a drug that is less burdensome to administer than FVIII preparations.

[0003] Occasionally, antibodies (inhibitors) against FVIII develop in hemophilia patients. Inhibitors counteract the effects of FVIII preparations. To treat bleeding in patients with inhibitors (inhibitor patients), bypassing agents are administered. Their mechanism of action is independent of the function of FVIII, namely, its function of catalyzing the activation of blood coagulation factor X (FX) by activated blood coagulation factor IX (FIXa). Therefore, there are cases in which bypassing agents are unable to sufficiently stop bleeding. Therefore, there has been a strong demand for a drug that is not affected by the presence of inhibitors and can replace the function of FVIII.

[0004] As a means for solving these problems, bispecific antibodies that substitute for the function of FVIII and their use have been reported (Patent Documents 1, 2, 3, and 4). Bispecific antibodies against FIXa and FX can substitute for the function of FVIII by positioning both factors in close proximity, thereby exerting FVIII cofactor functional substitution activity (Non-Patent Document 5). It has been reported that the FVIII cofactor functional substitution activity of the antibody can be improved by optimizing the affinity and orientation for FIXa and FX (Non-Patent Document 6). It is also known that the FVIII cofactor functional substitution activity of the antibody is affected by the IgG isotype, disulfide bond pattern, amino acid sequence of the hinge region, and the presence or absence of sugar chains in the Fc region (Non-Patent Document 7). It has been reported that ACE910 (Emicizumab), one of the antibodies and having high FVIII cofactor functional substitution activity, exhibits a hemostatic effect in a monkey hemophilia model (Non-Patent Documents 8 and 9). Furthermore, in clinical trials involving healthy subjects, ACE910 (Emicizumab) was confirmed to have excellent pharmacokinetics (long half-life) and tolerability (Non-Patent Document 10), and in clinical trials involving hemophilia A patients with and without inhibitors, administration of ACE910 (Emicizumab) was found to significantly reduce the number of bleeding episodes compared to before administration of ACE910 (Emicizumab) (Non-Patent Document 11).

[0005] Thus, ACE910 (Emicizumab) has been shown to have an effect of suppressing the number of bleeding episodes in clinical trials. However, the improvement effect of ACE910 (Emicizumab) in the maximum thrombin generation amount (Peak height) in an in vitro thrombin generation test using FVIII-deficient plasma was lower than that of the normal level of FVIII at 100 U / dL (Non-Patent Document 8). Therefore, further enhancement of the efficacy of the drug is desirable. In addition, in consideration of the convenience for patients with hemophilia A, a bispecific antibody having FVIII cofactor functional replacement activity that allows for further reduction in the dosage by improving the specific activity is desirable.

[0006] In general, antibody drugs may act as antigens and induce the production of anti-antibodies (ADA) (Non-Patent Document 12). For hemophilia patients who have developed ADA (anti-ACE910 (Emicizumab) idiotype antibodies), it becomes difficult to administer ACE910 (Emicizumab) continuously. Therefore, a bispecific antibody with FVIII cofactor functional replacement activity that can be administered to such patients is desired.

[0007] ACE910 (Emicizumab) is a bispecific antibody that has been optimized in many ways by introducing a large number of amino acid substitutions into the lead antibody hBS1 obtained by humanizing a bispecific antibody that recognizes FIX and / or FIXa and FX obtained by animal immunization, and has high FVIII cofactor functional replacement activity (Non-Patent Document 6, Patent Document 4). However, in order to enhance the efficacy and improve the specific activity, a bispecific antibody that replaces FVIII has a higher maximum activity (maximum activity of FVIII cofactor functional replacement activity) than ACE910 (Emicizumab) and can exert FVIII cofactor functional replacement activity at a lower concentration than ACE910 (Emicizumab). However, no bispecific antibody has been reported to date that has a significantly higher FVIII cofactor functional replacement activity in terms of maximum activity and concentration compared to ACE910 (Emicizumab) (Patent Documents 4 and 5).

[0008] Several methods have been reported so far as methods for producing IgG-type bispecific antibodies having a human constant region (IgG-type antibodies having a human constant region with binding specificity for antigen A in one arm and binding specificity for antigen B in the other arm). In general, IgG-type bispecific antibodies consist of two types of H chains (i.e., H chains for antigen A and H chains for antigen B) and two types of L chains (i.e., L chains for antigen A and L chains for antigen B). When expressing such an IgG-type bispecific antibody, two types of H chains and two types of L chains are expressed, so that there are ten possible combinations of H2L2. Of these, there is only one combination that has the desired binding specificity (IgG having binding specificity for antigen A in one arm and binding specificity for antigen B in the other arm). Therefore, in order to obtain the desired bispecific antibody, it is necessary to purify one type of desired antibody from the ten types of antibodies, which is extremely inefficient and difficult. As a method for solving this problem, a method has been reported in which amino acid substitutions are made in the CH3 region of the IgG H chain to preferentially secrete IgG with a heterologous combination of an H chain directed against antigen A and an H chain directed against antigen B (Patent Documents 6, 7, 8, 9 and Non-Patent Documents 13 and 14). These methods include a method that utilizes physical obstacles such as a "knob" and a "hole" and a method that utilizes electric charge repulsion. In order to obtain the target molecule more efficiently, a method has been reported in which a common L chain in which the L chain for antigen A and the L chain for antigen B have the same amino acid sequence has been used (Patent Documents 10 and 11). However, the use of a common L chain may significantly reduce the affinity for the antigen, and is not necessarily an optimal method. Therefore, in order for a bispecific antibody to bind to two antigens with strong affinity, it is preferable that only the H chain and L chain for antigen A are associated, and only the H chain and L chain for antigen B are associated. Furthermore, in order to associate the H chain and L chain for each antigen regardless of the variable region, a method has been reported in which amino acid substitutions are made in the CH1 and CL domains, which are constant regions, rather than in the variable region (Patent Documents 7, 12, and 13). However, there is still room for improvement in efficiently producing the desired bispecific antibody. [Prior art documents] [Patent documents]

[0009] [Patent Document 1] WO 2005 / 035754 [Patent Document 2] WO 2005 / 035756 [Patent Document 3] WO 2006 / 109592 [Patent Document 4] WO 2012 / 067176 [Patent Document 5] WO 2017 / 110980 [Patent Document 6] WO 1996 / 027011 [Patent Document 7] WO 2006 / 106905 [Patent Document 8] WO 2009 / 089004 [Patent Document 9] WO 2010 / 129304 [Patent Document 10] WO 98 / 050431 [Patent Document 11] WO 2006 / 109592

Patent document 12

Patent document 13

Non-licensed literature

[0010]

Non-licensed literature 1

Non-licensed Document 2

Non-licensed Document 4

Non-licensed Document 5

Non-licensed Document 6

Non-licensed Document 7

Non-licensed Document 8

Non-licensed literature 9

Non-licensed literature 10

Non-licensed Document 11

Non-licensed Document 12

Non-licensed Document 13

[0011] The present invention has been made in view of the above-mentioned circumstances, and an objective of the present invention is to provide a multispecific antigen-binding molecule that has blood coagulation factor VIII (FVIII) cofactor functional substituting activity, and a pharmaceutical preparation containing the molecule as an active ingredient. Furthermore, the present invention has been made in consideration of the above-mentioned circumstances, and an object of the present invention is to provide an antibody in which the association between the heavy chain and the light chain is controlled, a method for producing an antibody in which the association between the heavy chain and the light chain is controlled, and a method for controlling the association between the heavy chain and the light chain of an antibody. [Means for solving the problem]

[0012] In order to obtain a bispecific antibody with high maximum activity and specific activity of FVIII cofactor functional replacement activity, the inventors obtained a novel light chain with FVIII cofactor functional replacement activity from a human antibody library and a sequence different from that of ACE910 (Emicizumab), and produced a bispecific antibody by introducing amino acid substitutions at various sites in the light chain and the heavy chain of ACE910 (Emicizumab).They found that the increase in FVIII cofactor functional replacement activity was accompanied by an increase in blood coagulation factor IX (FIX) activation inhibitory activity. Therefore, the present inventors conducted extensive research and succeeded in finding a bispecific antibody that does not increase FIX activation inhibitory activity, but increases FVIII cofactor functional substituting activity. The present inventors also selected the heavy chain constant region CH1 and the light chain constant region (CL) as the heavy and light chain regions to be subjected to the control of the association, and conducted extensive research into the control of the association of these CH1 and CL. As a result, they succeeded in finding that undesirable association of CH1 and CL can be suppressed by substituting specific amino acid residues present at the interface between CH1 and CL with amino acid residues that repel each other electrically, and that heteromolecules are formed more efficiently than when the above-mentioned modification of introducing a knob and a hole into CH3 alone is used.

[0013] The present invention is based on such findings, and specifically provides the following [1] to

[25] . [1] A multispecific antigen-binding molecule having a function substituting for a function of blood coagulation factor VIII, comprising a first antigen-binding site that binds to blood coagulation factor IX and / or activated blood coagulation factor IX, and a second antigen-binding site that binds to blood coagulation factor X, the first antigen-binding site comprises a heavy chain variable domain and a light chain variable domain, the heavy chain variable domain (Q499) comprises HVR-H1 comprising the amino acid sequence of SEQ ID NO: 1, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 2, and HVR-H3 comprising the amino acid sequence of SEQ ID NO: 3; The light chain variable domain (QNK131) comprises HVR-L1 comprising the amino acid sequence of SEQ ID NO: 162, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 163, and HVR-L3 comprising the amino acid sequence of SEQ ID NO: 164; and the second antigen-binding site comprises a heavy chain variable domain and a light chain variable domain, the heavy chain variable domain (J327) comprises HVR-H1 comprising the amino acid sequence of SEQ ID NO: 4, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 5, and HVR-H3 comprising the amino acid sequence of SEQ ID NO: 6; The light chain variable domain (JNL095) comprises HVR-L1 comprising the amino acid sequence of SEQ ID NO: 165, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 166, and HVR-L3 comprising the amino acid sequence of SEQ ID NO: 167; In at least one of the HVRs, one or more amino acid residues are replaced with other amino acids, deleted, or inserted; The multispecific antigen-binding molecule. [2] at least one amino acid residue selected from amino acid residues at positions 31, 34, 97, 98, 100, 100a, 100b, and 100e according to the Kabat numbering in the heavy chain variable domain of the first antigen-binding site is substituted with another amino acid or deleted; at least one amino acid residue selected from amino acid residues at positions 26, 27, 30, 31, 32, 53, 55, 92, 93, 95, and 96 (Kabat numbering) in the light chain variable domain of the first antigen-binding site is substituted or inserted with another amino acid; at least one amino acid residue selected from amino acid residues at positions 31, 51, 56, 57, 59, 61, 62, 65, and 102 according to the Kabat numbering in the heavy chain variable domain of the second antigen-binding site is substituted with another amino acid; at least one amino acid residue selected from amino acid residues at positions 24, 26, 27, 29, 30, 31, 32, 50, 92, 94, 95, 95a, and 96 according to the Kabat numbering in the light chain variable domain of the second antigen-binding site is substituted with another amino acid or deleted; The multispecific antigen-binding molecule described in [1]. [3] in the heavy chain variable domain of the first antigen-binding site, the amino acid residue at position 31 according to the Kabat numbering is histidine, the amino acid residue at position 34 is alanine, the amino acid residue at position 97 is aspartic acid, the amino acid residue at position 98 is serine, the amino acid residue at position 100 is aspartic acid or glutamic acid, the amino acid residue at position 100a is aspartic acid or a deletion, the amino acid residue at position 100b is alanine or histidine, or the amino acid residue at position 100e is histidine or isoleucine; in the light chain variable domain of the first antigen-binding site, the amino acid residue at position 26 is threonine, the amino acid residue at position 27 is arginine, the amino acid residue at position 30 is arginine, the amino acid residue at position 31 is arginine, the amino acid residue at position 32 is aspartic acid or glutamic acid, the amino acid residue at position 53 is arginine, the amino acid residue at position 55 is glutamic acid, the amino acid residue at position 92 is arginine, the amino acid residue at position 93 is serine or aspartic acid, the amino acid residue at position 95 is proline, or the amino acid residue at position 96 is glycine, according to the Kabat numbering; in the heavy chain variable domain of the second antigen-binding site, the amino acid residue at position 31 is asparagine, glutamine or histidine, the amino acid residue at position 51 is serine, the amino acid residue at position 56 is threonine or arginine, the amino acid residue at position 57 is valine, the amino acid residue at position 59 is serine, the amino acid residue at position 61 is arginine, the amino acid residue at position 62 is lysine, the amino acid residue at position 65 is asparagine or glutamine, or the amino acid residue at position 102 is valine, according to the Kabat numbering; In the light chain variable domain of the second antigen-binding site, the amino acid residue at position 24 according to the Kabat numbering is threonine, the amino acid residue at position 26 is glutamic acid, the amino acid residue at position 27 is glutamine, the amino acid residue at position 29 is serine, the amino acid residue at position 30 is glutamine, serine or glutamic acid, the amino acid residue at position 31 is arginine, the amino acid residue at position 32 is glutamine or glutamic acid, the amino acid residue at position 50 is glutamine, the amino acid residue at position 92 is alanine, the amino acid residue at position 94 is aspartic acid, the amino acid residue at position 95 is aspartic acid or alanine, the amino acid residue at position 95a is tyrosine or deleted, or the amino acid residue at position 96 is threonine; The multispecific antigen-binding molecule of [1] or [2]. [4] the first antigen-binding site comprises a heavy chain variable domain and a light chain variable domain; In the heavy chain variable domain 1) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 168, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 169, HVR-H3 comprising the amino acid sequence of SEQ ID NO: 170 (QH01); 2) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 171, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 172, HVR-H3 comprising the amino acid sequence of SEQ ID NO: 173 (QH02); 3) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 174, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 175, HVR-H3 comprising the amino acid sequence of SEQ ID NO: 176 (QH03); 4) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 177, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 178, HVR-H3 comprising the amino acid sequence of SEQ ID NO: 179 (QH04); 5) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 180, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 181, HVR-H3 comprising the amino acid sequence of SEQ ID NO: 182 (QH06); or 6) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 183, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 184, HVR-H3 comprising the amino acid sequence of SEQ ID NO: 185 (QH07); and In the light chain variable domain, 1) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 186, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 187, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 188 (QL21); 2) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 189, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 190, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 191 (QL22); 3) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 192, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 193, and HVR-L3 comprising the amino acid sequence of SEQ ID NO: 194 (QL23); 4) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 195, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 196, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 197 (QL24); 5) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 198, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 199, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 200 (QL25); 6) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 201, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 202, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 203 (QL26); 7) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 204, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 205, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 206 (QL28); 8) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 207, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 208, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 209 (QL29); 9) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 210, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 211, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 212 (QL30); 10) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 213, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 214, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 215 (QL31); 11) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 216, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 217, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 218 (QL32); or 12) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 219, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 220, and HVR-L3 (QL33) comprising the amino acid sequence of SEQ ID NO: 221; and the second antigen-binding site comprises a heavy chain variable domain and a light chain variable domain; In the heavy chain variable domain 1) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 222, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 223, and HVR-H3 comprising the amino acid sequence of SEQ ID NO: 224 (JH01); 2) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 225, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 226, and HVR-H3 comprising the amino acid sequence of SEQ ID NO: 227 (JH02); 3) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 228, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 229, and HVR-H3 comprising the amino acid sequence of SEQ ID NO: 230 (JH03); 4) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 231, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 232, and HVR-H3 comprising the amino acid sequence of SEQ ID NO: 233 (JH04); 5) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 234, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 235, and HVR-H3 comprising the amino acid sequence of SEQ ID NO: 236 (JH05); 6) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 237, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 238, HVR-H3 comprising the amino acid sequence of SEQ ID NO: 239 (JH06); 7) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 240, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 241, HVR-H3 comprising the amino acid sequence of SEQ ID NO: 242 (JH07); 8) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 243, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 244, HVR-H3 comprising the amino acid sequence of SEQ ID NO: 245 (JH08); 9) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 246, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 247, HVR-H3 comprising the amino acid sequence of SEQ ID NO: 248 (JH09); 10) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 249, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 250, HVR-H3 comprising the amino acid sequence of SEQ ID NO: 251 (JH10); or 11) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 252, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 253, and HVR-H3 (JH11) comprising the amino acid sequence of SEQ ID NO: 254; and In the light chain variable domain, 1) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 255, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 256, and HVR-L3 comprising the amino acid sequence of SEQ ID NO: 257 (JL01); 2) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 258, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 259, and HVR-L3 comprising the amino acid sequence of SEQ ID NO: 260 (JL02); 3) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 261, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 262, and HVR-L3 comprising the amino acid sequence of SEQ ID NO: 263 (JL03); 4) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 264, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 265, and HVR-L3 comprising the amino acid sequence of SEQ ID NO: 266 (JL04); 5) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 267, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 268, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 269 (JL05); 6) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 270, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 271, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 272 (JL06); 7) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 273, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 274, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 275 (JL07); 8) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 276, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 277, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 278 (JL08); 9) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 279, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 280, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 281 (JL09); 10) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 282, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 283, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 284 (JL10); or 11) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 285, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 286, and HVR-L3 (JL11) comprising the amino acid sequence of SEQ ID NO: 287; The multispecific antigen-binding molecule according to any one of [1] to [3]. [5] The multispecific antigen-binding molecule of [1], wherein the first antigen-binding site comprises a heavy chain variable domain of SEQ ID NO: 45 (Q499) and a light chain variable domain of SEQ ID NO: 13 (QNK131), and the second antigen-binding site comprises a heavy chain variable domain of SEQ ID NO: 46 (J327) and a light chain variable domain of SEQ ID NO: 31 (JNL095), and one or more amino acid residues in at least one variable domain of the heavy chain variable domain or the light chain variable domain are substituted with other amino acids, deleted, or inserted. [6] at least one amino acid residue selected from amino acid residues at positions 31, 34, 39, 97, 98, 100, 100a, 100b, and 100e according to the Kabat numbering in the heavy chain variable domain of the first antigen-binding site is substituted with another amino acid or deleted; in the light chain variable domain of the first antigen-binding site, at least one amino acid residue selected from amino acid residues at positions 26, 27, 30, 31, 32, 38, 45, 53, 55, 60, 70, 76, 79, 80, 83, 85, 92, 93, 95, and 96 according to the Kabat numbering is substituted or inserted with another amino acid, in the heavy chain variable domain of the second antigen-binding site, at least one amino acid residue selected from amino acid residues at positions 28, 31, 39, 51, 56, 57, 59, 61, 62, 65, 67, 73, 82b, and 102 according to the Kabat numbering is substituted with another amino acid; in the light chain variable domain of the second antigen-binding site, at least one amino acid residue selected from amino acid residues at positions 3, 8, 15, 24, 26, 27, 29, 30, 31, 32, 38, 48, 49, 50, 79, 92, 94, 95, 95a, and 96 according to the Kabat numbering is substituted with another amino acid or deleted; The multispecific antigen-binding molecule described in [5]. [7] in the heavy chain variable domain of the first antigen-binding site, the amino acid residue at position 31 according to the Kabat numbering is histidine, the amino acid residue at position 34 is alanine, the amino acid residue at position 39 is glutamic acid, the amino acid residue at position 97 is aspartic acid, the amino acid residue at position 98 is serine, the amino acid residue at position 100 is aspartic acid or glutamic acid, the amino acid residue at position 100a is aspartic acid or a deletion, the amino acid residue at position 100b is alanine or histidine, or the amino acid residue at position 100e is histidine or isoleucine; In the light chain variable domain of the first antigen-binding site, the amino acid residue at position 26 according to the Kabat numbering is threonine, the amino acid residue at position 27 is arginine, the amino acid residue at position 30 is arginine, the amino acid residue at position 31 is arginine, the amino acid residue at position 32 is aspartic acid or glutamic acid, the amino acid residue at position 38 is lysine, the amino acid residue at position 45 is glutamic acid, the amino acid residue at position 53 is arginine, the amino acid residue at position 55 is glutamic acid, and the amino acid residue at position 60 is lysine. the amino acid residue at position 70 is aspartic acid, the amino acid residue at position 76 is asparagine, the amino acid residue at position 79 is glutamic acid, the amino acid residue at position 80 is proline or alanine, the amino acid residue at position 83 is methionine or alanine, the amino acid residue at position 85 is threonine, the amino acid residue at position 92 is arginine, the amino acid residue at position 93 is serine or aspartic acid, the amino acid residue at position 95 is proline, or the amino acid residue at position 96 is glycine; in the heavy chain variable domain of the second antigen-binding site, the amino acid residue at position 28 according to the Kabat numbering is glutamic acid, the amino acid residue at position 31 is asparagine, glutamine or histidine, the amino acid residue at position 39 is lysine, the amino acid residue at position 51 is serine, the amino acid residue at position 56 is threonine or arginine, the amino acid residue at position 57 is valine, the amino acid residue at position 59 is serine, the amino acid residue at position 61 is arginine, the amino acid residue at position 62 is lysine, the amino acid residue at position 65 is asparagine or glutamine, the amino acid residue at position 67 is leucine, the amino acid residue at position 73 is isoleucine, the amino acid residue at position 82b is glutamic acid, or the amino acid residue at position 102 is valine; In the light chain variable domain of the second antigen-binding site, the amino acid residue at position 3 according to the Kabat numbering is glutamic acid, the amino acid residue at position 8 is proline, the amino acid residue at position 15 is leucine, the amino acid residue at position 24 is threonine, the amino acid residue at position 26 is glutamic acid, the amino acid residue at position 27 is glutamine, the amino acid residue at position 29 is serine, the amino acid residue at position 30 is glutamine, serine or glutamic acid, the amino acid residue at position 31 is arginine, and the amino acid residue at position 32 is the amino acid residue at position 38 is glutamic acid, the amino acid residue at position 48 is isoleucine, the amino acid residue at position 49 is tyrosine, the amino acid residue at position 50 is glutamine, the amino acid residue at position 79 is glutamic acid, the amino acid residue at position 92 is alanine, the amino acid residue at position 94 is aspartic acid, the amino acid residue at position 95 is aspartic acid or alanine, the amino acid residue at position 95a is tyrosine or deleted, or the amino acid residue at position 96 is threonine; The multispecific antigen-binding molecule of [5] or [6]. [8] the first antigen-binding site comprises A heavy chain variable domain (QH) comprising the amino acid sequence set forth in SEQ ID NO: 56, SEQ ID NO: 57, SEQ ID NO: 58, SEQ ID NO: 59, or SEQ ID NO: 60; A light chain variable domain (QL) comprising the amino acid sequence set forth in SEQ ID NO:61, SEQ ID NO:62, SEQ ID NO:63, SEQ ID NO:64, SEQ ID NO:65, SEQ ID NO:66, SEQ ID NO:67, SEQ ID NO:68, SEQ ID NO:69, SEQ ID NO:70, SEQ ID NO:71 or SEQ ID NO:72, and the second antigen-binding site being A heavy chain variable domain (JH) comprising the amino acid sequence set forth in SEQ ID NO: 73, SEQ ID NO: 74, SEQ ID NO: 75, SEQ ID NO: 76, SEQ ID NO: 77, SEQ ID NO: 78, SEQ ID NO: 79, SEQ ID NO: 80, SEQ ID NO: 81, SEQ ID NO: 82 or SEQ ID NO: 83; A light chain variable domain (JL) comprising the amino acid sequence set forth in SEQ ID NO: 84, SEQ ID NO: 85, SEQ ID NO: 86, SEQ ID NO: 87, SEQ ID NO: 88, SEQ ID NO: 89, SEQ ID NO: 90, SEQ ID NO: 91, SEQ ID NO: 92, SEQ ID NO: 93 or SEQ ID NO: 94; The multispecific antigen-binding molecule according to any one of [1] to [7]. [9] The multispecific antigen-binding molecule according to any one of [1] to [8], wherein the first antigen-binding site comprises a constant region comprising the amino acid sequence described in the following (1) or (2), and the second antigen-binding site comprises a constant region comprising the amino acid sequence described in the following (1) or (2) that is different from the constant region contained in the first antigen-binding site: (1) SEQ ID NO: 119 as the heavy chain constant region and SEQ ID NO: 100 as the light chain constant region (2) SEQ ID NO: 118 as a heavy chain constant region and SEQ ID NO: 102 as a light chain constant region.

[10] The multispecific antigen-binding molecule of any one of [1] to [9], wherein the multispecific antigen-binding molecule is a multispecific antibody or a bispecific antibody.

[11] A bispecific antibody comprising a first antibody heavy chain and an antibody light chain which bind to blood coagulation factor IX and / or activated blood coagulation factor IX, and a second antibody heavy chain and an antibody light chain which bind to blood coagulation factor X, the bispecific antibody being any one of the following (a) to (v): (a) a bispecific antibody comprising a first antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 120, a first antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 126, a second antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 138, and a second antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 149 (QH01 / QL21 / / JH01 / JL01); (b) a bispecific antibody comprising a first antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 121, a first antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 127, a second antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 138, and a second antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 149 (QH02 / QL22 / / JH01 / JL01); (c) a bispecific antibody comprising a first antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 122, a first antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 128, a second antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 139, and a second antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 150 (QH03 / QL23 / / JH02 / JL02); (d) a bispecific antibody comprising a first antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 122, a first antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 129, a second antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 139, and a second antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 150 (QH03 / QL24 / / JH02 / JL02); (e) a bispecific antibody comprising a first antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 121, a first antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 127, a second antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 140, and a second antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 151 (QH02 / QL22 / / JH03 / JL03); (f) a bispecific antibody comprising a first antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 121, a first antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 127, a second antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 141, and a second antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 152 (QH02 / QL22 / / JH04 / JL04); (g) a bispecific antibody comprising a first antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 121, a first antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 127, a second antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 139, and a second antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 150 (QH02 / QL22 / / JH02 / JL02); (h) a bispecific antibody comprising a first antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 123, a first antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 130, a second antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 139, and a second antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 150 (QH04 / QL25 / / JH02 / JL02); (i) a bispecific antibody comprising a first antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 123, a first antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 131, a second antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 139, and a second antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 150 (QH04 / QL26 / / JH02 / JL02); (j) a bispecific antibody comprising a first antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 123, a first antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 131, a second antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 142, and a second antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 153 (QH04 / QL26 / / JH05 / JL05); (k) a bispecific antibody comprising a first antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 123, a first antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 132, a second antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 142, and a second antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 153 (QH04 / QL28 / / JH05 / JL05); (l) a bispecific antibody comprising a first antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 123, a first antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 132, a second antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 143, and a second antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 154 (QH04 / QL28 / / JH06 / JL06); (m) a bispecific antibody comprising a first antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 123, a first antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 133, a second antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 142, and a second antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 153 (QH04 / QL29 / / JH05 / JL05); (n) a bispecific antibody comprising a first antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 123, a first antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 133, a second antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 143, and a second antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 154 (QH04 / QL29 / / JH06 / JL06); (o) a bispecific antibody comprising a first antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 124, a first antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 134, a second antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 144, and a second antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 155 (QH06 / QL30 / / JH07 / JL07); (p) a bispecific antibody comprising a first antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 123, a first antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 135, a second antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 145, and a second antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 156 (QH04 / QL31 / / JH08 / JL08); (q) a bispecific antibody comprising a first antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 124, a first antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 136, a second antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 144, and a second antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 155 (QH06 / QL32 / / JH07 / JL07); (r) a bispecific antibody comprising a first antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 124, a first antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 136, a second antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 146, and a second antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 157 (QH06 / QL32 / / JH09 / JL09); (s) a bispecific antibody comprising a first antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 124, a first antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 134, a second antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 147, and a second antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 158 (QH06 / QL30 / / JH10 / JL10); (t) a bispecific antibody comprising a first antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 125, a first antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 137, a second antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 148, and a second antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 159 (QH07 / QL33 / / JH11 / JL11); (u) a bispecific antibody that binds to the same epitope in both blood coagulation factor IX and / or activated blood coagulation factor IX and the same epitope in blood coagulation factor X as those recognized by any of the antibodies (a) to (t); (v) A bispecific antibody that competes for binding to an epitope in blood coagulation factor IX and / or activated blood coagulation factor IX recognized by any of the antibodies (a) to (t) and an epitope in blood coagulation factor X, respectively.

[12] An antigen-binding molecule in which the association of a heavy chain and a light chain is controlled, The antigen-binding molecule, in which one or more pairs of amino acid residues selected from the group consisting of the pairs of amino acid residues shown in the following (a) to (c) in the heavy chain and the light chain are amino acid residues that repel each other electrically: (a) an amino acid residue at position 175 (EU numbering) contained in the heavy chain constant region (CH1) and an amino acid residue at position 180 (Kabat numbering) contained in the light chain constant region (CL); (b) an amino acid residue at position 175 (EU numbering) contained in CH1, and an amino acid residue at position 131 (Kabat numbering) contained in CL; (c) amino acid residues at positions 147 and 175 in the EU numbering system contained in CH1, and amino acid residues at positions 131 and 180 in the Kabat numbering system contained in CL.

[13] The antigen-binding molecule of

[12] , further comprising two or more amino acid residues forming an interface between the heavy chain variable region and the light chain variable region that are mutually electrically repulsive.

[14] The antigen-binding molecule of

[13] , wherein the mutually charge-repulsive amino acid residues are one or two pairs of amino acid residues selected from the group consisting of the pairs of amino acid residues shown in (a) or (b) below: (a) an amino acid residue at position 39 (Kabat numbering) contained in a heavy chain variable region, and an amino acid residue at position 38 (Kabat numbering) contained in a light chain variable region; (b) the amino acid residue at position 45 (Kabat numbering) contained in the heavy chain variable region, and the amino acid residue at position 44 (Kabat numbering) contained in the light chain variable region.

[15] The antigen-binding molecule according to any one of

[12] to

[14] , wherein the mutually charge-repulsive amino acid residues are selected from amino acid residues included in either pair (X) or (Y) below: (X) glutamic acid (E), aspartic acid (D), (Y) Lysine (K), Arginine (R), Histidine (H).

[16] The antigen-binding molecule of any of

[12] to

[15] , wherein the antigen-binding molecule is a bispecific antibody.

[17] A method for producing an antigen-binding molecule in which the association of heavy and light chains is controlled, comprising the following steps (1) to (3): (1) modifying nucleic acids encoding a heavy chain constant region (CH1) and a light chain constant region (CL) so that one or more pairs of amino acid residues selected from the group consisting of the pairs of amino acid residues shown in the following (a) to (c) are mutually electrically repelled; (a) an amino acid residue at position 175 in the EU numbering system contained in CH1, and an amino acid residue at position 180 in the Kabat numbering system contained in CL; (b) an amino acid residue at position 175 in the EU numbering system contained in CH1, and an amino acid residue at position 131 in the Kabat numbering system contained in CL; (c) amino acid residues at positions 147 and 175 (EU numbering) in CH1, and amino acid residues at positions 131 and 180 (Kabat numbering) in CL (2) introducing the modified nucleic acid into a host cell and culturing the host cell so that the nucleic acid is expressed; (3) recovering the antigen-binding molecule from the host cell culture.

[18] A method for controlling the association of a heavy chain and a light chain of an antigen-binding molecule, comprising the steps of: A method comprising modifying a nucleic acid so that one or more pairs of amino acid residues selected from the group consisting of the pairs of amino acid residues shown in the following (a) to (c) are amino acid residues that are mutually repulsive in charge: (a) an amino acid residue at position 175 in the EU numbering system contained in CH1, and an amino acid residue at position 180 in the Kabat numbering system contained in CL; (b) an amino acid residue at position 175 (EU numbering) contained in CH1, and an amino acid residue at position 131 (Kabat numbering) contained in CL; (c) amino acid residues at positions 147 and 175 in the EU numbering system contained in CH1, and amino acid residues at positions 131 and 180 in the Kabat numbering system contained in CL.

[19] An isolated nucleic acid encoding the multispecific antigen-binding molecule of any of [1] to [9], the multispecific antibody of

[10] , the bispecific antibody of

[10] ,

[11] , or

[16] , or the antigen-binding molecule of any of

[12] to

[15] .

[20] A host cell comprising the nucleic acid described in

[19] . 〔twenty one〕 A method for producing a multispecific antigen-binding molecule, a multispecific antibody, a bispecific antibody or an antigen-binding molecule, the method comprising culturing the host cell of

[20] so that the multispecific antigen-binding molecule, the multispecific antibody, the bispecific antibody or the antigen-binding molecule is produced. 〔twenty two〕 A pharmaceutical formulation comprising the multispecific antigen-binding molecule of any of [1] to [9], the multispecific antibody of

[10] , the bispecific antibody of

[10] ,

[11] , or

[16] , or the antigen-binding molecule of any of

[12] to

[15] , and a pharma- ceutical acceptable carrier. 〔twenty three〕 The pharmaceutical preparation according to

[22] , which is used for the prevention and / or treatment of bleeding, a disease accompanied by bleeding, or a disease caused by bleeding. 〔twenty four〕 The pharmaceutical preparation according to

[23] , wherein the bleeding, disease accompanied by bleeding, or disease caused by bleeding is a disease that develops and / or progresses due to a decrease in or deficiency of activity of blood coagulation factor VIII and / or activated blood coagulation factor VIII. 〔twenty five〕 The pharmaceutical preparation according to

[24] , wherein the disease which develops and / or progresses due to a decrease in or deficiency in the activity of blood coagulation factor VIII and / or activated blood coagulation factor VIII is hemophilia A, a disease in which inhibitors against blood coagulation factor VIII and / or activated blood coagulation factor VIII have appeared, acquired hemophilia, or von Willebrand's disease.

[0014] The present invention also relates to the following:

[26] The multispecific antigen-binding molecule of any one of [1] to [9], the multispecific antibody of

[10] , the bispecific antibody of

[10] ,

[11] or

[16] , or the antigen-binding molecule of any one of

[12] to

[15] , for use as a pharmaceutical.

[27] The multispecific antigen-binding molecule of any of [1] to [9], the multispecific antibody of

[10] , or the bispecific antibody of

[10] or

[11] for use in the prevention and / or treatment of bleeding, a disease accompanied by bleeding, or a disease caused by bleeding.

[28] The multispecific antigen-binding molecule of any one of [1] to [9], the multispecific antibody of

[10] , or the bispecific antibody of

[10] or

[11] , for use in functional replacement of a FVIII cofactor.

[29] Use of the multispecific antigen-binding molecule of any of [1] to [9], the multispecific antibody of

[10] , or the bispecific antibody of

[10] or

[11] in the manufacture of a pharmaceutical for the treatment of bleeding, a disease accompanied by bleeding, or a disease caused by bleeding.

[30] Use of the multispecific antigen-binding molecule of any of [1] to [9], the multispecific antibody of

[10] , or the bispecific antibody of

[10] or

[11] in the manufacture of a pharmaceutical for replacing a FVIII cofactor function.

[31] A method for treating an individual having bleeding, a disease accompanied by bleeding, or a disease caused by bleeding, comprising administering to the individual an effective amount of the multispecific antigen-binding molecule of any of [1] to [9], the multispecific antibody of

[10] , or the bispecific antibody of

[10] or

[11] .

[32] A method for replacing a FVIII cofactor function in an individual, the method comprising the step of administering to the individual an effective amount of the multispecific antigen-binding molecule of any of [1] to [9], the multispecific antibody of

[10] , or the bispecific antibody of

[10] or

[11] , so as to replace the FVIII cofactor function.

[0015] The present invention also relates to the following:

[33] The method for producing an antigen-binding molecule of

[17] , comprising the step of modifying a nucleic acid in the step (1) so that the amino acid residues that repel each other in terms of charge are selected from amino acid residues included in either group (X) or (Y) below: (X) glutamic acid (E), aspartic acid (D), (Y) Lysine (K), Arginine (R), Histidine (H).

[34] The method for producing an antigen-binding molecule according to

[17] or

[33] , further comprising a step of modifying the nucleic acid in step (1) so that two or more amino acid residues forming an interface between the heavy chain variable region and the light chain variable region are amino acid residues that repel each other in terms of charge.

[35] the method for producing an antigen-binding molecule of

[34] , wherein the mutually charge-repulsive amino acid residues are any pair of amino acid residues selected from the group consisting of the pairs of amino acid residues shown in (a) or (b) below: (a) an amino acid residue at position 39 (Kabat numbering) contained in a heavy chain variable region, and an amino acid residue at position 38 (Kabat numbering) contained in a light chain variable region; (b) the amino acid residue at position 45 (Kabat numbering) contained in the heavy chain variable region, and the amino acid residue at position 44 (Kabat numbering) contained in the light chain variable region.

[36] the method for producing the antigen-binding molecule of

[34] or

[35] , wherein the mutually charge-repulsive amino acid residues are selected from amino acid residues included in either pair (X) or (Y) below; (X) glutamic acid (E), aspartic acid (D), (Y) Lysine (K), Arginine (R), Histidine (H).

[37] The method according to

[18] , wherein the mutually charge-repulsive amino acid residues are selected from amino acid residues included in either of the following groups (X) and (Y): (X) glutamic acid (E), aspartic acid (D), (Y) Lysine (K), Arginine (R), Histidine (H).

[38] The method according to

[18] or

[37] , further comprising: two or more amino acid residues forming an interface between the heavy chain variable region and the light chain variable region that are mutually electrically repulsive.

[39] The method according to

[38] , wherein the mutually charge-repulsive amino acid residues are any one pair of amino acid residues selected from the group consisting of the pairs of amino acid residues shown in the following (a) or (b): (a) an amino acid residue at position 39 (Kabat numbering) contained in a heavy chain variable region, and an amino acid residue at position 38 (Kabat numbering) contained in a light chain variable region; (b) the amino acid residue at position 45 (Kabat numbering) contained in the heavy chain variable region, and the amino acid residue at position 44 (Kabat numbering) contained in the light chain variable region.

[40] The method according to

[38] or

[39] , wherein the mutually charge-repulsive amino acid residues are selected from amino acid residues included in either of the following pairs (X) or (Y): (X) glutamic acid (E), aspartic acid (D), (Y) Lysine (K), Arginine (R), Histidine (H).

[0016] The present invention also relates to the following:

[41] The multispecific antigen-binding molecule of any of [1] to [7], which is described in any of (a) to (v) below: (a) a multispecific antigen-binding molecule comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 56, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 61, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 73, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 84. (QH01 / QL21 / / JH01 / JL01); (b) a multispecific antigen-binding molecule comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 57, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 62, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 73, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 84 (QH02 / QL22 / / JH01 / JL01); (c) a multispecific antigen-binding molecule comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 58, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 63, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 74, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 85 (QH03 / QL23 / / JH02 / JL02); (d) a multispecific antigen-binding molecule comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 58, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 64, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 74, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 85 (QH03 / QL24 / / JH02 / JL02); (e) a multispecific antigen-binding molecule comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 57, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 62, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 75, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 86 (QH02 / QL22 / / JH03 / JL03); (f) a multispecific antigen-binding molecule comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 57, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 62, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 76, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 87 (QH02 / QL22 / / JH04 / JL04); (g) a multispecific antigen-binding molecule comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 57, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 62, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 74, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 85 (QH02 / QL22 / / JH02 / JL02); (h) a multispecific antigen-binding molecule comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 59, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 65, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 74, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 85 (QH04 / QL25 / / JH02 / JL02); (i) a multispecific antigen-binding molecule comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 59, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 66, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 74, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 85 (QH04 / QL26 / / JH02 / JL02); (j) a multispecific antigen-binding molecule comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 59, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 66, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 77, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 88 (QH04 / QL26 / / JH05 / JL05); (k) a multispecific antigen-binding molecule comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 59, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 67, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 77, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 88 (QH04 / QL28 / / JH05 / JL05); (l) a multispecific antigen-binding molecule comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 59, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 67, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 78, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 89 (QH04 / QL28 / / JH06 / JL06); (m) a multispecific antigen-binding molecule comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 59, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 68, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 77, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 88 (QH04 / QL29 / / JH05 / JL05); (n) a multispecific antigen-binding molecule comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 59, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 68, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 78, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 89 (QH04 / QL29 / / JH06 / JL06); (o) a multispecific antigen-binding molecule comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 60, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 69, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 79, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 90 (QH06 / QL30 / / JH07 / JL07); (p) a multispecific antigen-binding molecule comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 59, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 70, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 80, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 91 (QH04 / QL31 / / JH08 / JL08); (q) a multispecific antigen-binding molecule comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 60, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 71, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 79, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 90 (QH06 / QL32 / / JH07 / JL07); (r) a multispecific antigen-binding molecule comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 60, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 71, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 81, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 92 (QH06 / QL32 / / JH09 / JL09); (s) a multispecific antigen-binding molecule comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 60, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 69, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 82, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 93 (QH06 / QL30 / / JH10 / JL10); (t) a multispecific antigen-binding molecule comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 105, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 72, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 83, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 94 (QH07 / QL33 / / JH11 / JL11); (u) a multispecific antigen-binding molecule that binds to the same epitope as both the epitope in blood coagulation factor IX and / or activated blood coagulation factor IX and the epitope in blood coagulation factor X recognized by any of the antibodies (a) to (t); (v) A multispecific antigen-binding molecule that competes for binding to an epitope in blood coagulation factor IX and / or activated blood coagulation factor IX recognized by any of the antibodies (a) to (t) and an epitope in blood coagulation factor X, respectively.

[42] A bispecific antibody according to any of the following (a) to (v), comprising a first antibody heavy chain variable domain and an antibody light chain variable domain which bind to blood coagulation factor IX and / or activated blood coagulation factor IX, and a second antibody heavy chain variable domain and an antibody light chain variable domain which bind to blood coagulation factor X: (a) a bispecific antibody comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 56, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 61, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 73, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 84. (QH01 / QL21 / / JH01 / JL01); (b) a bispecific antibody comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 57, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 62, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 73, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 84. (QH02 / QL22 / / JH01 / JL01); (c) a bispecific antibody comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 58, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 63, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 74, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 85. (QH03 / QL23 / / JH02 / JL02); (d) a bispecific antibody comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 58, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 64, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 74, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 85. (QH03 / QL24 / / JH02 / JL02); (e) a bispecific antibody comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 57, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 62, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 75, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 86. (QH02 / QL22 / / JH03 / JL03); (f) a bispecific antibody comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 57, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 62, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 76, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 87. (QH02 / QL22 / / JH04 / JL04); (g) a bispecific antibody comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 57, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 62, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 74, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 85. (QH02 / QL22 / / JH02 / JL02); (h) a bispecific antibody comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 59, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 65, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 74, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 85. (QH04 / QL25 / / JH02 / JL02); (i) a bispecific antibody comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 59, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 66, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 74, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 85. (QH04 / QL26 / / JH02 / JL02); (j) a bispecific antibody comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 59, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 66, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 77, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 88. (QH04 / QL26 / / JH05 / JL05); (k) a bispecific antibody comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 59, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 67, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 77, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 88. (QH04 / QL28 / / JH05 / JL05); (l) a bispecific antibody comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 59, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 67, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 78, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 89. (QH04 / QL28 / / JH06 / JL06); (m) a bispecific antibody comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 59, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 68, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 77, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 88. (QH04 / QL29 / / JH05 / JL05); (n) a bispecific antibody comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 59, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 68, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 78, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 89. (QH04 / QL29 / / JH06 / JL06); (o) a bispecific antibody comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 60, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 69, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 79, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 90. (QH06 / QL30 / / JH07 / JL07); (p) a bispecific antibody comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 59, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 70, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 80, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 91. (QH04 / QL31 / / JH08 / JL08); (q) a bispecific antibody comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 60, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 71, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 79, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 90. (QH06 / QL32 / / JH07 / JL07); (r) a bispecific antibody comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 60, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 71, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 81, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 92. (QH06 / QL32 / / JH09 / JL09); (s) a bispecific antibody comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 60, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 69, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 82, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 93. (QH06 / QL30 / / JH10 / JL10); (t) a bispecific antibody comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 105, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 72, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 83, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 94. (QH07 / QL33 / / JH11 / JL11); (u) a bispecific antibody that binds to the same epitope in both blood coagulation factor IX and / or activated blood coagulation factor IX and the same epitope in blood coagulation factor X as those recognized by any of the antibodies (a) to (t); (v) A bispecific antibody that competes for binding to an epitope in blood coagulation factor IX and / or activated blood coagulation factor IX recognized by any of the antibodies (a) to (t) and an epitope in blood coagulation factor X, respectively. [Brief description of the drawings]

[0017] [Figure 1] FIG. 1 shows the reactivity of bispecific antibodies having novel L chains with anti-ACE910 (Emicizumab) idiotype antibodies. [Diagram 2] FIG. 1 shows the effect that FIX activation inhibitory activity may have on FX activation. [Diagram 3] FIG. 1 is a plot of the FVIII cofactor functional replacement activity and FIX activation inhibitory activity of novel L chain and H chain modifications. [Figure 4-1] FIG. 1 shows the concentration dependence of FVIII cofactor functional substituting activity of bispecific antibodies combining H-chain and L-chain modifications. [Figure 4-2] FIG. 1 shows the concentration dependence of FVIII cofactor functional substituting activity of bispecific antibodies combining H-chain and L-chain modifications. [Diagram 5] FIG. 1 shows the maximum amount of thrombin generated (peak height) in a thrombin generation test for bispecific antibodies that combine H-chain and L-chain modifications. [Figure 6] FIG. 1 shows ECM binding of the bispecific antibodies produced. [Figure 7] FIG. 1 shows antibody PK of the bispecific antibodies prepared. [Figure 8] FIG. 1 shows the types of mispairs that can occur. [Figure 9-1] FIG. 1 shows CIEX analysis data of the prepared bispecific antibodies. [Figure 9-2]FIG. 1 shows CIEX analysis data of the prepared bispecific antibodies. [Figure 9-3] FIG. 1 shows CIEX analysis data of the prepared bispecific antibodies. [Figure 9-4] FIG. 1 shows CIEX analysis data of the prepared bispecific antibodies. [Figure 9-5] FIG. 1 shows CIEX analysis data of the prepared bispecific antibodies. [Figure 9-6] FIG. 1 shows CIEX analysis data of the prepared bispecific antibodies. [Figure 9-7] FIG. 1 shows CIEX analysis data of the prepared bispecific antibodies. [Figure 9-8] FIG. 1 shows CIEX analysis data of the prepared bispecific antibodies. [Figure 9-9] FIG. 1 shows CIEX analysis data of the prepared bispecific antibodies. [Figure 9-10] FIG. 1 shows CIEX analysis data of the prepared bispecific antibodies. [Figure 9-11] FIG. 1 shows CIEX analysis data of the prepared bispecific antibodies. [Figure 9-12] FIG. 1 shows CIEX analysis data of the prepared bispecific antibodies. [Figure 9-13] FIG. 1 shows CIEX analysis data of the prepared bispecific antibodies. [Figure 9-14] FIG. 1 shows CIEX analysis data of the prepared bispecific antibodies. [Figure 9-15] FIG. 1 shows CIEX analysis data of the prepared bispecific antibodies. [Figure 9-16] FIG. 1 shows CIEX analysis data of the prepared bispecific antibodies. [Figure 9-17] FIG. 1 shows CIEX analysis data of the prepared bispecific antibodies. [Figure 9-18] FIG. 1 shows CIEX analysis data of the prepared bispecific antibodies. [Figure 9-19] FIG. 1 shows CIEX analysis data of the prepared bispecific antibodies. [Figure 9-20]FIG. 1 shows CIEX analysis data of the prepared bispecific antibodies. [Figure 10] FIG. 1 shows the results of measuring the FVIII cofactor functional substituting activity of bispecific antibodies having novel L chains. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0018] I. Definition An "acceptor human framework" for the purposes of this specification is a framework that comprises the amino acid sequence of a light chain variable domain (VL) framework or a heavy chain variable domain (VH) framework derived from a human immunoglobulin framework or a human consensus framework as defined below. An acceptor human framework "derived" from a human immunoglobulin framework or a human consensus framework may comprise those same amino acid sequences or may contain amino acid sequence changes. In some embodiments, the number of amino acid changes is 10 or less, 9 or less, 8 or less, 7 or less, 6 or less, 5 or less, 4 or less, 3 or less, or 2 or less. In some embodiments, the VL acceptor human framework is identical in sequence to the VL human immunoglobulin framework sequence or the human consensus framework sequence.

[0019] "Affinity" refers to the strength of the total non-covalent interactions between one binding site of a molecule (e.g., an antibody) and the molecule's binding partner (e.g., an antigen). Unless otherwise indicated, "binding affinity" as used herein refers to the inherent binding affinity that reflects a 1:1 interaction between members of a binding pair (e.g., an antibody and an antigen). The affinity of a molecule X for its partner Y can generally be expressed by the dissociation constant (Kd). Affinity can be measured by conventional methods known in the art, including those described herein. Specific illustrative and exemplary embodiments for measuring binding affinity are described below.

[0020] An "affinity matured" antibody refers to an antibody with one or more modifications in one or more hypervariable regions (HVRs) that result in improved affinity of the antibody for antigen, compared to a parent antibody that does not possess the modifications.

[0021] The multispecific antigen-binding molecules described herein comprise a first antigen-binding site and a second antigen-binding site capable of specifically binding to at least two different antigens. In one embodiment, the first antigen-binding site and the second antigen-binding site may each have binding activity to blood coagulation factor IX (FIX) and / or activated blood coagulation factor IX (FIXa) and blood coagulation factor X (FX), and examples of such sites include sites necessary for binding to antigens in antibodies, scaffold molecules (antibody-like molecules), peptides, etc., or fragments containing such sites. A scaffold molecule is a molecule that exerts its function by binding to a target molecule, and any polypeptide can be used as long as it is a conformationally stable polypeptide that can bind to at least one target antigen. Examples of such polypeptides include antibody variable regions, fibronectin (WO2002 / 032925), Protein A domain (WO1995 / 001937), LDL receptor A domain (WO2004 / 044011, WO2005 / 040229), ankyrin (WO2002 / 020565), and the like, as well as molecules described in Nygren et al. (Current Opinion in Structural Biology, 7:463-469(1997); Journal of Immunol Methods, 290:3-28(2004)), Binz et al. (Nature Biotech 23:1257-1266(2005)), and Hosse et al. (Protein Science 15:14-27(2006)). Furthermore, peptide molecules capable of binding to target antigens can also be used, as described in Curr Opin Mol Ther. 2010 Aug;12(4):487-95. and Drugs. 2008;68(7):901-12.

[0022] The polypeptide of the present invention generally refers to a peptide or protein having a length of about 10 amino acids or more. Generally, the polypeptide is derived from an organism, but is not particularly limited thereto, and may be, for example, a polypeptide consisting of an artificially designed sequence. The polypeptide may be any of a natural polypeptide, a synthetic polypeptide, a recombinant polypeptide, and the like. Furthermore, fragments of the above polypeptides are also included in the polypeptide of the present invention.

[0023] In one embodiment, the multispecific antigen-binding molecule can include a multispecific antibody that can specifically bind to at least two different antigens, or to two different epitopes of the same antigen. In one embodiment, a multispecific antibody of the present invention is a bispecific antibody (BsAb) (sometimes called a bispecific antibody).

[0024] As used herein, "FVIII cofactor functional replacement activity," "FVIII replacement activity," and "activity replacing the function of FVIII" are used synonymously and refer to the activity of binding to FIX and / or FIXa and FX and promoting the activation of FX (promoting the production of activated blood coagulation factor X (FXa)).

[0025] In one embodiment, the multispecific antigen-binding molecule having FVIII cofactor functional replacement activity is a multispecific antigen-binding molecule that binds to FIX and / or FIXa and FX.

[0026] In one particular embodiment, the multispecific antigen-binding molecule with FVIII cofactor functional replacement activity is a multispecific antibody that binds to FIX and / or FIXa and FX. Also, in another particular embodiment, the multispecific antibody that binds FIX and / or FIXa and FX is a bispecific antibody that binds FIX and / or FIXa and FX.

[0027] The term "bispecific antibody that binds FIX and / or FIXa and FX" refers to a bispecific antibody that is capable of binding FIX and / or FIXa and FX with sufficient affinity such that the antibody is useful as a diagnostic and / or therapeutic agent when targeted to FIX and / or FIXa and FX. In one embodiment, the extent of binding of a bispecific antibody that binds FIX and / or FIXa and FX to an unrelated non-FIX, non-FIXa or non-FX protein is less than about 10% of the binding of the antibody to FIX and / or FIXa and FX as measured (e.g., by radioimmunoassay (RIA)). In certain embodiments, antibodies that bind FIX and / or FIXa and FX have a binding affinity of ≦100 μM, ≦10 μM, ≦1 μM, ≦100 nM, ≦10 nM, ≦1 nM, ≦0.1 nM, ≦0.01 nM, or ≦0.001 nM (e.g., 10 -5 M or less, e.g. 10 -5 M~10 -10 M, for example, 10 -6 M~10 -10 In certain embodiments, the bispecific antibody that binds FIX and / or FIXa and FX binds to an epitope of FIX, FIXa and FX that is conserved between FIX, FIXa and FX, respectively, from different species.

[0028] In the present invention, the term "antibody" is used synonymously with "antigen-binding molecule" in a specific embodiment. In the present invention, the term "antibody" or "antigen-binding molecule" is used in the broadest sense, and includes monoclonal antibodies, polyclonal antibodies, and antibody mutants (chimeric antibodies, humanized antibodies, minibodies (including antibody fragments to which other molecules may be added), multispecific antibodies, etc.) as long as they exhibit the desired antigen-binding activity and biological activity. For example, the "antibody" or "antigen-binding molecule" in the present invention includes a molecule in which a HAS-binding scaffold is added to Fab (only the Fab portion is a normal antibody). In addition, in the present invention, the "antibody" may be either a polypeptide or a heterologous multimer. Preferred antibodies are monoclonal antibodies, chimeric antibodies, humanized antibodies, human antibodies, Fc-fusion antibodies, and minibodies such as antibody fragments. As used herein, an "antibody" refers to a binding protein that comprises an antigen-binding site. The term "binding site" or "antigen-binding site" as used herein denotes the region of an antibody molecule where an antigen actually binds. The term "antigen-binding site" comprises an antibody heavy chain variable domain (VH) and an antibody light chain variable domain (VL) (VH / VL pair).

[0029] An "antibody fragment" refers to a molecule other than a complete antibody that contains a portion of the complete antibody that binds to the antigen to which the complete antibody binds. Examples of antibody fragments include, but are not limited to, Fv, Fab, Fab', Fab'-SH, F(ab') 2 , diabodies, linear antibodies, single-chain antibody molecules (e.g., scFv), and multispecific antibodies formed from antibody fragments.

[0030] An "antibody that binds to the same epitope" as a reference antibody refers to an antibody that blocks the binding of the reference antibody to its own antigen by 50% or more in a competitive assay, and conversely, the reference antibody blocks the binding of the antibody to its own antigen by 50% or more in a competitive assay. Exemplary competitive assays are provided herein.

[0031] The term "chimeric" antibody refers to an antibody in which a portion of the heavy and / or light chain is derived from a particular source or species, while the remainder of the heavy and / or light chain is derived from a different source or species.

[0032] The "class" of an antibody refers to the type of constant domain or constant region present in the antibody's heavy chain. There are five major classes of antibodies: IgA, IgD, IgE, IgG, and IgM. Some of these may be further divided into subclasses (isotypes), e.g., IgG1, IgG2, IgG3, IgG4, IgA1, and IgA2. The heavy chain constant domains that correspond to the different classes of immunoglobulins are called α, δ, ε, γ, and μ, respectively.

[0033] An "effective amount" of an agent (eg, a pharmaceutical formulation) refers to the amount, at dosages and for periods of time necessary, effective to achieve the desired therapeutic or prophylactic result.

[0034] The term "Fc region" is used herein to define a C-terminal region of an immunoglobulin heavy chain that includes at least a portion of the constant region. This term includes native sequence Fc regions and variant Fc regions. In one embodiment, a human IgG heavy chain Fc region extends from Cys226 or from Pro230 to the carboxyl terminus of the heavy chain, except that the C-terminal lysine (Lys447) or glycine-lysine (Gly446-Lys447) of the Fc region may or may not be present. Unless otherwise specified herein, the numbering of amino acid residues in the Fc region or constant region is according to the EU numbering system (also referred to as the EU index) as described in Kabat et al., Sequences of Proteins of Immunological Interest, 5th Ed. Public Health Service, National Institutes of Health, Bethesda, MD 1991.

[0035] "Framework" or "FR" refers to variable domain residues other than hypervariable region (HVR) residues. The FR of a variable domain typically consists of four FR domains: FR1, FR2, FR3, and FR4. Accordingly, the HVR and FR sequences typically appear in VH (or VL) in the following order: FR1-H1(L1)-FR2-H2(L2)-FR3-H3(L3)-FR4.

[0036] The terms "full length antibody," "complete antibody," and "whole antibody" are used interchangeably herein and refer to an antibody having a structure substantially similar to a native antibody structure or having a heavy chain that includes an Fc region as defined herein.

[0037] The terms "host cell," "host cell line," and "host cell culture" are used interchangeably and refer to cells into which exogenous nucleic acid has been introduced, including the progeny of such cells. Host cells include "transformants" and "transformed cells," which include the originally transformed cell and progeny derived from that cell regardless of the number of passages. The progeny may not be completely identical in nucleic acid content to the parent cell and may contain mutations. Also included herein are mutant progeny that have the same function or biological activity as that for which the original transformed cell was screened or selected.

[0038] A "human antibody" is an antibody with an amino acid sequence that corresponds to that of an antibody produced by a human or a human cell, or derived from a human antibody repertoire or other non-human source that uses human antibody coding sequences. This definition of a human antibody specifically excludes humanized antibodies, which contain non-human antigen-binding residues.

[0039] A "human consensus framework" is a framework that represents the most commonly occurring amino acid residues in a selection of human immunoglobulin VL or VH framework sequences. Usually, the selection of human immunoglobulin VL or VH sequences is from a subgroup of variable domain sequences. Usually, the subgroup of sequences is a subgroup in Kabat et al., Sequences of Proteins of Immunological Interest, Fifth Edition, NIH Publication 91-3242, Bethesda MD (1991), vols. 1-3. In one embodiment, for VL, the subgroup is subgroup kappa I according to Kabat et al., supra. In one embodiment, for VH, the subgroup is subgroup III according to Kabat et al., supra.

[0040] A "humanized" antibody refers to a chimeric antibody that contains amino acid residues from non-human HVRs and amino acid residues from human FRs. In certain embodiments, a humanized antibody contains substantially all of at least one, and typically two, variable domains, in which all or substantially all HVRs (e.g., CDRs) correspond to those of a non-human antibody and all or substantially all FRs correspond to those of a human antibody. A humanized antibody may optionally contain at least a portion of an antibody constant region derived from a human antibody. A "humanized form" of an antibody (e.g., a non-human antibody) refers to an antibody that has undergone humanization.

[0041] The term "hypervariable region" or "HVR" as used herein refers to each region of an antibody variable domain that is hypervariable in sequence (the "complementarity determining region" or "CDR") and / or forms structurally defined loops (the "hypervariable loops") and / or contains antigen contact residues (the "antigen contacts"). Typically, antibodies contain six HVRs: three in the VH (H1, H2, H3) and three in the VL (L1, L2, L3). Exemplary HVRs herein include the following: (a) hypervariable loops occurring at amino acid residues 26-32 (L1), 50-52 (L2), 91-96 (L3), 26-32 (H1), 53-55 (H2), and 96-101 (H3) (Chothia and Lesk, J. Mol. Biol. 196:901-917 (1987)); (b) CDRs occurring at amino acid residues 24-34 (L1), 50-56 (L2), 89-97 (L3), 31-35b (H1), 50-65 (H2), and 95-102 (H3) (Kabat et al., Sequences of Proteins of Immunological Interest, 5th Ed. Public Health Service, National Institutes of Health, Bethesda, MD (1991)); (c) antigenic contacts occurring at amino acid residues 27c-36 (L1), 46-55 (L2), 89-96 (L3), 30-35b (H1), 47-58 (H2), and 93-101 (H3) (MacCallum et al. J. Mol. Biol. 262: 732-745 (1996)); and (d) combinations of (a), (b), and / or (c), comprising HVR amino acid residues 46-56 (L2), 47-56 (L2), 48-56 (L2), 49-56 (L2), 26-35 (H1), 26-35b (H1), 49-65 (H2), 93-102 (H3), and 94-102 (H3). Unless otherwise indicated, HVR residues and other residues in the variable domain (eg, FR residues) are numbered herein according to Kabat et al., supra.

[0042] An "immunoconjugate" is an antibody conjugated to one or more heterologous molecules, including but not limited to, cytotoxic agents.

[0043] An "individual" or "subject" is a mammal. Mammals include, but are not limited to, domestic animals (e.g., cows, sheep, cats, dogs, horses), primates (e.g., humans and non-human primates such as monkeys), rabbits, and rodents (e.g., mice and rats). In certain embodiments, the individual or subject is a human.

[0044] An "isolated" antibody is one that has been separated from the components of its original environment. In some embodiments, the antibody is purified to greater than 95% or 99% purity, for example, as measured by electrophoresis (e.g., SDS-PAGE, isoelectric focusing (IEF), capillary electrophoresis) or chromatography (e.g., ion exchange or reverse phase HPLC). For a review of methods for assessing antibody purity, see, for example, Flatman et al., J. Chromatogr. B 848:79-87 (2007).

[0045] An "isolated" nucleic acid refers to a nucleic acid molecule that has been separated from a component of its original environment. Isolated nucleic acid includes a nucleic acid molecule contained in a cell that normally contains the nucleic acid molecule, but where the nucleic acid molecule is present extrachromosomally or in a chromosomal location that is different from its natural chromosomal location.

[0046] "Isolated nucleic acid encoding a bispecific antibody that binds FIX and / or FIXa and FX" refers to one or more nucleic acid molecules encoding the antibody heavy and light chains (or fragments thereof), including nucleic acid molecules carried on a single vector or separate vectors, and including nucleic acid molecules present in one or more locations in a host cell.

[0047] As used herein, the term "monoclonal antibody" refers to an antibody obtained from a population of substantially homogeneous antibodies. That is, the individual antibodies that make up the population are identical and / or bind to the same epitope, except for possible variant antibodies (e.g., variant antibodies including naturally occurring variants or variant antibodies that arise during the manufacture of a monoclonal antibody preparation, which are usually present in small amounts). In contrast to polyclonal antibody preparations, which typically contain different antibodies against different determinants (epitopes), each monoclonal antibody of a monoclonal antibody preparation is directed against a single determinant on an antigen. Thus, the modifier "monoclonal" indicates the character of the antibody as being obtained from a population of substantially homogeneous antibodies, and should not be construed as requiring production of the antibody by any particular method. For example, monoclonal antibodies used in accordance with the present invention may be made by a variety of techniques, including, but not limited to, hybridoma techniques, recombinant DNA techniques, phage display techniques, and methods utilizing transgenic animals containing all or part of the human immunoglobulin loci, and such methods and other exemplary methods for making monoclonal antibodies are described herein.

[0048] "Native antibodies" refer to immunoglobulin molecules with various structures that occur naturally. For example, native IgG antibodies are heterotetrameric glycoproteins of about 150,000 daltons composed of two identical light chains and two identical heavy chains that are disulfide-bonded. From the N-terminus to the C-terminus, each heavy chain has a variable region (VH), also called the variable heavy domain or the heavy chain variable domain, followed by three constant domains (CH1, CH2, and CH3). Similarly, from the N-terminus to the C-terminus, each light chain has a variable region (VL), also called the variable light domain or the light chain variable domain, followed by a constant light (CL) domain. The light chain of an antibody may be assigned to one of two types, called kappa (κ) and lambda (λ), based on the amino acid sequence of its constant domain.

[0049] The term "package insert" is used to refer to instructions typically included in commercial packaging of a therapeutic product that contain information about the indications, usage, dosage, method of administration, concomitant therapy, contraindications, and / or warnings regarding the use of such therapeutic product.

[0050] "Percent (%) amino acid sequence identity" to a reference polypeptide sequence is defined as the percentage of amino acid residues in a candidate sequence that are identical to amino acid residues in the reference polypeptide sequence after aligning the sequences to obtain the maximum percent sequence identity and introducing gaps if necessary, and not considering any conservative substitutions as part of the sequence identity. Alignment for the purpose of determining percent amino acid sequence identity can be achieved by various methods within the skill of the art, for example, using publicly available computer software such as BLAST, BLAST-2, ALIGN, Megalign (DNASTAR) software, or GENETYX (registered trademark) (Genetyx Co., Ltd.). Those skilled in the art can determine appropriate parameters for aligning sequences, including any algorithms required to achieve maximum alignment over the entire length of the sequences being compared.

[0051] The ALIGN-2 sequence comparison computer program is the copyright of Genentech, Inc., and its source code has been filed with user documentation in the US Copyright Office, Washington DC, 20559, where it is registered under US Copyright Registration No. TXU510087. The ALIGN-2 program is publicly available from Genentech, Inc., South San Francisco, California, or may be compiled from the source code. The ALIGN-2 program is compiled for use on UNIX operating systems, including Digital UNIX V4.0D. All sequence comparison parameters are set by the ALIGN-2 program and do not vary. In the context of using ALIGN-2 for amino acid sequence comparison, the % amino acid sequence identity of a given amino acid sequence A to, with, or against a given amino acid sequence B (alternatively, one may say a given amino acid sequence A has or contains a certain % amino acid sequence identity to, with, or against a given amino acid sequence B) is calculated as follows: 100 times the fraction X / Y, where X is the number of amino acid residues scored as identical matches by the sequence alignment program ALIGN-2 in said program's alignment of A and B, and Y is the total number of amino acid residues in B. It will be understood that if the length of amino acid sequence A is not equal to the length of amino acid sequence B, the % amino acid sequence identity of A to B is not equal to the % amino acid sequence identity of B to A. Unless otherwise specified, all % amino acid sequence identity values ​​used herein are obtained using the ALIGN-2 computer program as described in the immediately preceding paragraph.

[0052] The term "pharmaceutical formulation" refers to a preparation in a form such that the biological activity of the active ingredients contained therein can be exerted, and which does not contain additional components that are unacceptably toxic to the subject to which the formulation is administered.

[0053] A "pharmaceutically acceptable carrier" refers to an ingredient in a pharmaceutical formulation, other than an active ingredient, that is non-toxic to a subject. Pharmaceutically acceptable carriers include, but are not limited to, buffers, excipients, stabilizers, or preservatives.

[0054] As used herein, the terms "FIX", "FIXa" and "FX", unless otherwise indicated, refer to any native "FIX", native "FIXa" and native "FX" from any vertebrate source, including mammals such as primates (e.g., humans) and rodents (e.g., mice and rats). The terms encompass "full-length", unprocessed "FIX", "FIXa" and "FX", as well as any form of "FIX", "FIXa" and "FX" that results from processing in the cell. The terms also encompass naturally occurring variants of "FIX", "FIXa" and "FX", such as splice variants and allelic variants.

[0055] As used herein, "treatment" (and its grammatical derivatives, such as "treat", "treating", etc.) refers to a clinical intervention intended to modify the natural course of the individual being treated, and may be performed prophylactically or during the course of a clinical condition. Desirable effects of treatment include, but are not limited to, prevention of disease onset or recurrence, alleviation of symptoms, attenuation of any direct or indirect pathological effects of the disease, prevention of metastasis, reduction in the rate of disease progression, amelioration or palliation of the disease state, and remission or improved prognosis. In some embodiments, the antibodies of the invention are used to delay disease onset or slow disease progression.

[0056] The term "variable region" or "variable domain" refers to the domain of an antibody's heavy or light chain that is involved in binding the antibody to an antigen. The heavy and light chain variable domains (VH and VL, respectively) of natural antibodies usually have a similar structure, with each domain containing four conserved framework regions (FR) and three hypervariable regions (HVR). (See, for example, Kindt et al. Kuby Immunology, 6th ed., WH Freeman and Co., page 91 (2007).) One VH or VL domain may be sufficient to confer antigen-binding specificity. Furthermore, antibodies that bind to a particular antigen may be isolated by screening a complementary library of VL or VH domains, respectively, with a VH or VL domain from an antibody that binds to the antigen. See, e.g., Portolano et al., J. Immunol. 150:880-887 (1993); Clarkson et al., Nature 352:624-628 (1991).

[0057] As used herein, the term "vector" refers to a nucleic acid molecule capable of propagating another nucleic acid to which it is linked. This term includes vectors as self-replicating nucleic acid structures and vectors that are integrated into the genome of a host cell into which it is introduced. A vector can effect expression of a nucleic acid to which it is operatively linked. Such vectors are also referred to herein as "expression vectors."

[0058] II. Compositions and Methods In one aspect, the present invention is based in part on a multispecific antigen-binding molecule with FVIII cofactor functional replacement activity. In a particular embodiment, a multispecific antigen-binding molecule is provided that binds to FIX and / or FIXa and FX. The multispecific antigen-binding molecule of the present invention is useful, for example, for the treatment of bleeding, bleeding disorders, or bleeding disorders.

[0059] A. Exemplary multispecific antigen-binding molecules that bind FIX and / or FIXa and FX In one aspect, the present invention provides an isolated multispecific antigen-binding molecule that binds to FIX and / or FIXa and FX. In a particular embodiment, the multispecific antigen-binding molecule is a bispecific antibody that binds to FIX and / or FIXa and FX, and the bispecific antibody has FVIII cofactor functional replacement activity.

[0060] ACE910 (Emicizumab), a bispecific antibody that binds to FIX and / or FIXa and FX, is the following antibody described in the patent document (WO 2012 / 067176): A bispecific antibody in which a first polypeptide and a third polypeptide form a pair and a second polypeptide and a fourth polypeptide form a pair, wherein the first polypeptide is an H chain comprising the amino acid sequences of HVR-H1, HVR-H2, and HVR-H3 set forth in SEQ ID NOs: 1, 2, and 3 (Q499 H chain CDR), the second polypeptide is an H chain comprising the amino acid sequences of HVR-H1, HVR-H2, and HVR-H3 set forth in SEQ ID NOs: 4, 5, and 6 (J327 H chain CDR), and the third polypeptide and the fourth polypeptide are a common L chain comprising the amino acid sequences of HVR-L1, HVR-L2, and HVR-L3 set forth in SEQ ID NOs: 7, 8, and 9 (L chain CDR of L404). More specifically, this is a bispecific antibody in which a first polypeptide and a third polypeptide form a pair and a second polypeptide and a fourth polypeptide form a pair, wherein the first polypeptide is an H chain comprising the amino acid sequence of the H chain variable region set forth in SEQ ID NO: 45, the second polypeptide is an H chain comprising the amino acid sequence of the H chain variable region set forth in SEQ ID NO: 46, and the third polypeptide and the fourth polypeptide are a common L chain comprising the amino acid sequence of the L chain variable region set forth in SEQ ID NO: 47. More specifically, the present invention relates to a bispecific antibody in which a first polypeptide and a third polypeptide form a pair and a second polypeptide and a fourth polypeptide form a pair, wherein the first polypeptide is an H chain consisting of the amino acid sequence set forth in SEQ ID NO: 10, the second polypeptide is an H chain consisting of the amino acid sequence set forth in SEQ ID NO: 11, and the third polypeptide and the fourth polypeptide are a common L chain consisting of the common L chain set forth in SEQ ID NO: 12 (Q499-z121 / J327-z119 / L404-k).

[0061] The multispecific antigen-binding molecule of the present invention is a multispecific antigen-binding molecule having a function substituting for the function of FVIII, comprising a first antigen-binding site that binds to FIX and / or FIXa and a second antigen-binding site that binds to FX, the first antigen-binding site comprises a heavy chain variable domain and a light chain variable domain, the heavy chain variable domain (Q499) comprises HVR-H1 comprising the amino acid sequence of SEQ ID NO: 1, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 2, and HVR-H3 comprising the amino acid sequence of SEQ ID NO: 3; The light chain variable domain (QNK131) comprises HVR-L1 comprising the amino acid sequence of SEQ ID NO: 162, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 163, and HVR-L3 comprising the amino acid sequence of SEQ ID NO: 164; and the second antigen-binding site comprises a heavy chain variable domain and a light chain variable domain, the heavy chain variable domain (J327) comprises HVR-H1 comprising the amino acid sequence of SEQ ID NO: 4, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 5, and HVR-H3 comprising the amino acid sequence of SEQ ID NO: 6; The light chain variable domain (JNL095) comprises HVR-L1 comprising the amino acid sequence of SEQ ID NO: 165, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 166, and HVR-L3 comprising the amino acid sequence of SEQ ID NO: 167; A multispecific antigen-binding molecule, wherein one or more amino acid residues in at least one of the HVRs are substituted with other amino acids, deleted or inserted.

[0062] In a specific embodiment, the multispecific antigen-binding molecule of the present invention is a multispecific antigen-binding molecule having a function that substitutes for the function of FVIII. In another embodiment, the multispecific antigen-binding molecule of the present invention is a multispecific antigen-binding molecule having reduced reactivity with anti-ACE910 (Emicizumab) idiotype antibody compared to ACE910 (Emicizumab). Here, "reactivity with anti-ACE910 (Emicizumab) idiotype antibody is reduced compared to ACE910 (Emicizumab)" means that when the binding strength (%) between the anti-idiotype antibody and labeled ACE910 is measured using the method described in Example 1, the binding strength is preferably reduced by 10% or more, more preferably reduced by 20% or more, and even more preferably reduced by 30% or more at a concentration of the antibody to be measured of 100 μg / mL. In another aspect, the multispecific antigen-binding molecule has enhanced FVIII cofactor functional substituting activity compared to ACE910 (Emicizumab), and has high activity even at a low antibody concentration. In another embodiment, the multispecific antigen-binding molecule has substantially no FIX activation inhibitory activity and has increased FVIII cofactor functional substituting activity compared to ACE910 (Emicizumab). Here, "has substantially no FIX activation inhibitory activity" means that when the OD value is measured using the method described in the Examples, the decrease in OD value compared to the OD value of the control is within 0.025, preferably within 0.02, and more preferably within 0.01.

[0063] In one embodiment, the heavy chain variable domain of the first antigen-binding site of the multispecific antigen-binding molecule of the present invention is a heavy chain variable domain of the first antigen-binding site in which at least one amino acid residue selected from the amino acid residues at positions 31, 34, 97, 98, 100, 100a, 100b, and 100e according to the Kabat numbering in the heavy chain variable domain of the first antigen-binding site is substituted with another amino acid or deleted. In one embodiment, the light chain variable domain of the first antigen-binding site of the present invention is a light chain variable domain of the first antigen-binding site in which at least one amino acid residue selected from amino acid residues at positions 26, 27, 30, 31, 32, 53, 55, 92, 93, 95, and 96 according to the Kabat numbering in the light chain variable domain of the first antigen-binding site has been substituted or inserted with another amino acid. In one embodiment, the heavy chain variable domain of the second antigen-binding site of the present invention is a heavy chain variable domain of the second antigen-binding site described above in which at least one amino acid residue selected from the amino acid residues at positions 31, 51, 56, 57, 59, 61, 62, 65, and 102 according to the Kabat numbering in the heavy chain variable domain of the second antigen-binding site has been substituted with another amino acid. In one embodiment, the light chain variable domain of the second antigen-binding site of the present invention is a light chain variable domain of the second antigen-binding site in which at least one amino acid residue selected from the amino acid residues at positions 24, 26, 27, 29, 30, 31, 32, 50, 92, 94, 95, 95a, and 96 according to the Kabat numbering in the light chain variable domain of the second antigen-binding site described above is substituted with another amino acid or is deleted.

[0064] In one embodiment, a multispecific antigen-binding molecule of the invention comprises: at least one amino acid residue selected from amino acid residues at positions 31, 34, 97, 98, 100, 100a, 100b, and 100e according to the Kabat numbering in the heavy chain variable domain of the first antigen-binding site is substituted with another amino acid or deleted; at least one amino acid residue selected from amino acid residues at positions 26, 27, 30, 31, 32, 53, 55, 92, 93, 95, and 96 (Kabat numbering) in the light chain variable domain of the first antigen-binding site is substituted or inserted with another amino acid; at least one amino acid residue selected from amino acid residues at positions 31, 51, 56, 57, 59, 61, 62, 65, and 102 according to the Kabat numbering in the heavy chain variable domain of the second antigen-binding site is substituted with another amino acid; at least one amino acid residue selected from amino acid residues at positions 24, 26, 27, 29, 30, 31, 32, 50, 92, 94, 95, 95a, and 96 according to the Kabat numbering in the light chain variable domain of the second antigen-binding site is substituted with another amino acid or deleted; It is a multispecific antigen-binding molecule.

[0065] In one embodiment, the heavy chain variable domain of the first antigen-binding site of the multispecific antigen-binding molecule of the present invention is a heavy chain variable domain of the first antigen-binding site in which the amino acid residue at position 31 is histidine, the amino acid residue at position 34 is alanine, the amino acid residue at position 97 is aspartic acid, the amino acid residue at position 98 is serine, the amino acid residue at position 100 is aspartic acid or glutamic acid, the amino acid residue at position 100a is aspartic acid or deleted, the amino acid residue at position 100b is alanine or histidine, or the amino acid residue at position 100e is histidine or isoleucine, according to the Kabat numbering in the heavy chain variable domain of the first antigen-binding site described above. In one embodiment, the light chain variable domain of the first antigen-binding site of the present invention is a light chain variable domain of the first antigen-binding site in which the amino acid residue at position 26 is threonine, the amino acid residue at position 27 is arginine, the amino acid residue at position 30 is arginine, the amino acid residue at position 31 is arginine, the amino acid residue at position 32 is aspartic acid or glutamic acid, the amino acid residue at position 53 is arginine, the amino acid residue at position 55 is glutamic acid, the amino acid residue at position 92 is arginine, the amino acid residue at position 93 is serine or aspartic acid, the amino acid residue at position 95 is proline, or the amino acid residue at position 96 is glycine, according to the Kabat numbering. In one embodiment, the heavy chain variable domain of the second antigen-binding site of the present invention is a heavy chain variable domain of the second antigen-binding site in which the amino acid residue at position 31, according to the Kabat numbering, is asparagine, glutamine or histidine, the amino acid residue at position 51 is serine, the amino acid residue at position 56 is threonine or arginine, the amino acid residue at position 57 is valine, the amino acid residue at position 59 is serine, the amino acid residue at position 61 is arginine, the amino acid residue at position 62 is lysine, the amino acid residue at position 65 is asparagine or glutamine, or the amino acid residue at position 102 is valine. In one embodiment, the light chain variable domain of the second antigen-binding site of the present invention is a light chain variable domain of the second antigen-binding site in which the amino acid residue at position 24 is threonine, the amino acid residue at position 26 is glutamic acid, the amino acid residue at position 27 is glutamine, the amino acid residue at position 29 is serine, the amino acid residue at position 30 is glutamine, serine or glutamic acid, the amino acid residue at position 31 is arginine, the amino acid residue at position 32 is glutamine or glutamic acid, the amino acid residue at position 50 is glutamine, the amino acid residue at position 92 is alanine, the amino acid residue at position 94 is aspartic acid, the amino acid residue at position 95 is aspartic acid or alanine, the amino acid residue at position 95a is tyrosine or deleted, or the amino acid residue at position 96 is threonine, according to the Kabat numbering.

[0066] In one embodiment, a multispecific antigen-binding molecule of the invention comprises: in the heavy chain variable domain of the first antigen-binding site, the amino acid residue at position 31 according to the Kabat numbering is histidine, the amino acid residue at position 34 is alanine, the amino acid residue at position 97 is aspartic acid, the amino acid residue at position 98 is serine, the amino acid residue at position 100 is aspartic acid or glutamic acid, the amino acid residue at position 100a is aspartic acid or a deletion, the amino acid residue at position 100b is alanine or histidine, or the amino acid residue at position 100e is histidine or isoleucine; in the light chain variable domain of the first antigen-binding site, the amino acid residue at position 26 is threonine, the amino acid residue at position 27 is arginine, the amino acid residue at position 30 is arginine, the amino acid residue at position 31 is arginine, the amino acid residue at position 32 is aspartic acid or glutamic acid, the amino acid residue at position 53 is arginine, the amino acid residue at position 55 is glutamic acid, the amino acid residue at position 92 is arginine, the amino acid residue at position 93 is serine or aspartic acid, the amino acid residue at position 95 is proline, or the amino acid residue at position 96 is glycine, according to the Kabat numbering; in the heavy chain variable domain of the second antigen-binding site, the amino acid residue at position 31 is asparagine, glutamine or histidine, the amino acid residue at position 51 is serine, the amino acid residue at position 56 is threonine or arginine, the amino acid residue at position 57 is valine, the amino acid residue at position 59 is serine, the amino acid residue at position 61 is arginine, the amino acid residue at position 62 is lysine, the amino acid residue at position 65 is asparagine or glutamine, or the amino acid residue at position 102 is valine, according to the Kabat numbering; In the light chain variable domain of the second antigen-binding site, the amino acid residue at position 24 according to the Kabat numbering is threonine, the amino acid residue at position 26 is glutamic acid, the amino acid residue at position 27 is glutamine, the amino acid residue at position 29 is serine, the amino acid residue at position 30 is glutamine, serine or glutamic acid, the amino acid residue at position 31 is arginine, the amino acid residue at position 32 is glutamine or glutamic acid, the amino acid residue at position 50 is glutamine, the amino acid residue at position 92 is alanine, the amino acid residue at position 94 is aspartic acid, the amino acid residue at position 95 is aspartic acid or alanine, the amino acid residue at position 95a is tyrosine or deleted, or the amino acid residue at position 96 is threonine; It is a multispecific antigen-binding molecule.

[0067] In one embodiment, the heavy chain variable domain of the first antigen-binding site of the multispecific antigen-binding molecule of the invention comprises 1) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 168, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 169, HVR-H3 comprising the amino acid sequence of SEQ ID NO: 170 (QH01); 2) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 171, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 172, HVR-H3 comprising the amino acid sequence of SEQ ID NO: 173 (QH02); 3) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 174, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 175, HVR-H3 comprising the amino acid sequence of SEQ ID NO: 176 (QH03); 4) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 177, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 178, HVR-H3 comprising the amino acid sequence of SEQ ID NO: 179 (QH04); 5) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 180, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 181, HVR-H3 comprising the amino acid sequence of SEQ ID NO: 182 (QH06); or 6) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 183, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 184, and HVR-H3 comprising the amino acid sequence of SEQ ID NO: 185 (QH07) and a heavy chain variable domain comprising: In one embodiment, the light chain variable domain of the first antigen-binding site of the multispecific antigen-binding molecule of the invention comprises 1) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 186, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 187, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 188 (QL21); 2) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 189, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 190, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 191 (QL22); 3) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 192, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 193, and HVR-L3 comprising the amino acid sequence of SEQ ID NO: 194 (QL23); 4) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 195, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 196, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 197 (QL24); 5) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 198, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 199, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 200 (QL25); 6) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 201, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 202, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 203 (QL26); 7) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 204, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 205, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 206 (QL28); 8) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 207, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 208, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 209 (QL29); 9) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 210, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 211, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 212 (QL30); 10) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 213, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 214, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 215 (QL31); 11) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 216, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 217, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 218 (QL32); or 12) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 219, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 220, and HVR-L3 comprising the amino acid sequence of SEQ ID NO: 221 (QL33) and a light chain variable domain comprising: In one embodiment, the heavy chain variable domain of the second antigen-binding site of the multispecific antigen-binding molecule of the invention comprises 1) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 222, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 223, and HVR-H3 comprising the amino acid sequence of SEQ ID NO: 224 (JH01); 2) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 225, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 226, and HVR-H3 comprising the amino acid sequence of SEQ ID NO: 227 (JH02); 3) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 228, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 229, and HVR-H3 comprising the amino acid sequence of SEQ ID NO: 230 (JH03); 4) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 231, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 232, and HVR-H3 comprising the amino acid sequence of SEQ ID NO: 233 (JH04); 5) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 234, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 235, and HVR-H3 comprising the amino acid sequence of SEQ ID NO: 236 (JH05); 6) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 237, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 238, HVR-H3 comprising the amino acid sequence of SEQ ID NO: 239 (JH06); 7) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 240, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 241, HVR-H3 comprising the amino acid sequence of SEQ ID NO: 242 (JH07); 8) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 243, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 244, HVR-H3 comprising the amino acid sequence of SEQ ID NO: 245 (JH08); 9) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 246, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 247, HVR-H3 comprising the amino acid sequence of SEQ ID NO: 248 (JH09); 10) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 249, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 250, HVR-H3 comprising the amino acid sequence of SEQ ID NO: 251 (JH10); or 11) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 252, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 253, and HVR-H3 comprising the amino acid sequence of SEQ ID NO: 254 (JH11) and a heavy chain variable domain comprising: In one embodiment, the light chain variable domain of the second antigen-binding site of the multispecific antigen-binding molecule of the invention comprises 1) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 255, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 256, and HVR-L3 comprising the amino acid sequence of SEQ ID NO: 257 (JL01); 2) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 258, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 259, and HVR-L3 comprising the amino acid sequence of SEQ ID NO: 260 (JL02); 3) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 261, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 262, and HVR-L3 comprising the amino acid sequence of SEQ ID NO: 263 (JL03); 4) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 264, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 265, and HVR-L3 comprising the amino acid sequence of SEQ ID NO: 266 (JL04); 5) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 267, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 268, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 269 (JL05); 6) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 270, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 271, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 272 (JL06); 7) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 273, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 274, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 275 (JL07); 8) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 276, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 277, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 278 (JL08); 9) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 279, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 280, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 281 (JL09); 10) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 282, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 283, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 284 (JL10); or 11) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 285, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 286, and HVR-L3 comprising the amino acid sequence of SEQ ID NO: 287 (JL11) and a light chain variable domain comprising:

[0068] In one embodiment, the multispecific antigen-binding molecule of the present invention comprises a first antigen-binding site comprising a heavy chain variable domain and a light chain variable domain, In the heavy chain variable domain 1) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 168, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 169, HVR-H3 comprising the amino acid sequence of SEQ ID NO: 170 (QH01); 2) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 171, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 172, HVR-H3 comprising the amino acid sequence of SEQ ID NO: 173 (QH02); 3) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 174, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 175, HVR-H3 comprising the amino acid sequence of SEQ ID NO: 176 (QH03); 4) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 177, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 178, HVR-H3 comprising the amino acid sequence of SEQ ID NO: 179 (QH04); 5) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 180, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 181, HVR-H3 comprising the amino acid sequence of SEQ ID NO: 182 (QH06); or 6) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 183, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 184, and HVR-H3 (QH07) comprising the amino acid sequence of SEQ ID NO: 185; and In the light chain variable domain, 1) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 186, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 187, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 188 (QL21); 2) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 189, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 190, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 191 (QL22); 3) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 192, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 193, and HVR-L3 comprising the amino acid sequence of SEQ ID NO: 194 (QL23); 4) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 195, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 196, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 197 (QL24); 5) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 198, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 199, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 200 (QL25); 6) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 201, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 202, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 203 (QL26); 7) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 204, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 205, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 206 (QL28); 8) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 207, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 208, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 209 (QL29); 9) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 210, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 211, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 212 (QL30); 10) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 213, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 214, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 215 (QL31); 11) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 216, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 217, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 218 (QL32); or 12) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 219, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 220, and HVR-L3 (QL33) comprising the amino acid sequence of SEQ ID NO: 221; and the second antigen-binding site comprises a heavy chain variable domain and a light chain variable domain; In the heavy chain variable domain 1) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 222, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 223, and HVR-H3 comprising the amino acid sequence of SEQ ID NO: 224 (JH01); 2) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 225, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 226, and HVR-H3 comprising the amino acid sequence of SEQ ID NO: 227 (JH02); 3) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 228, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 229, and HVR-H3 comprising the amino acid sequence of SEQ ID NO: 230 (JH03); 4) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 231, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 232, and HVR-H3 comprising the amino acid sequence of SEQ ID NO: 233 (JH04); 5) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 234, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 235, and HVR-H3 comprising the amino acid sequence of SEQ ID NO: 236 (JH05); 6) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 237, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 238, HVR-H3 comprising the amino acid sequence of SEQ ID NO: 239 (JH06); 7) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 240, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 241, HVR-H3 comprising the amino acid sequence of SEQ ID NO: 242 (JH07); 8) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 243, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 244, HVR-H3 comprising the amino acid sequence of SEQ ID NO: 245 (JH08); 9) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 246, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 247, HVR-H3 comprising the amino acid sequence of SEQ ID NO: 248 (JH09); 10) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 249, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 250, HVR-H3 comprising the amino acid sequence of SEQ ID NO: 251 (JH10); or 11) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 252, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 253, and HVR-H3 (JH11) comprising the amino acid sequence of SEQ ID NO: 254; and In the light chain variable domain, 1) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 255, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 256, and HVR-L3 comprising the amino acid sequence of SEQ ID NO: 257 (JL01); 2) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 258, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 259, and HVR-L3 comprising the amino acid sequence of SEQ ID NO: 260 (JL02); 3) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 261, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 262, and HVR-L3 comprising the amino acid sequence of SEQ ID NO: 263 (JL03); 4) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 264, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 265, and HVR-L3 comprising the amino acid sequence of SEQ ID NO: 266 (JL04); 5) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 267, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 268, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 269 (JL05); 6) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 270, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 271, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 272 (JL06); 7) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 273, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 274, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 275 (JL07); 8) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 276, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 277, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 278 (JL08); 9) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 279, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 280, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 281 (JL09); 10) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 282, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 283, HVR-L3 comprising the amino acid sequence of SEQ ID NO: 284 (JL10); or 11) HVR-L1 comprising the amino acid sequence of SEQ ID NO: 285, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 286, and HVR-L3 (JL11) comprising the amino acid sequence of SEQ ID NO: 287; It is a multispecific antigen-binding molecule.

[0069] In one embodiment of the present invention, the multispecific antigen-binding molecule of the present invention is a multispecific antigen-binding molecule having a function substituting for the function of blood coagulation factor VIII, comprising a first antigen-binding site that binds to blood coagulation factor IX and / or activated blood coagulation factor IX, and a second antigen-binding site that binds to blood coagulation factor X, The first antigen-binding site comprises a heavy chain variable domain of SEQ ID NO: 45 (Q499) and a light chain variable domain of SEQ ID NO: 13 (QNK131), and the second antigen-binding site comprises a heavy chain variable domain of SEQ ID NO: 46 (J327) and a light chain variable domain of SEQ ID NO: 31 (JNL095), and one or more amino acid residues in at least one variable domain of the heavy chain variable domain or the light chain variable domain are substituted with other amino acids or deleted.

[0070] In one embodiment, the heavy chain variable domain of the first antigen-binding site of the present invention is a heavy chain variable domain in which at least one amino acid residue selected from amino acid residues at positions 31, 34, 39, 97, 98, 100, 100a, 100b, and 100e according to the Kabat numbering in the heavy chain variable domain of the first antigen-binding site is substituted with another amino acid or deleted. In one embodiment, the light chain variable domain of the first antigen-binding site of the present invention is a light chain variable domain in which at least one amino acid residue selected from amino acid residues at positions 26, 27, 30, 31, 32, 38, 45, 53, 55, 60, 70, 76, 79, 80, 83, 85, 92, 93, 95, and 96 according to the Kabat numbering in the light chain variable domain of the first antigen-binding site has been substituted or inserted with another amino acid. In one embodiment, the heavy chain variable domain of the second antigen-binding site of the present invention is a heavy chain variable domain in which at least one amino acid residue selected from amino acid residues at positions 28, 31, 39, 51, 56, 57, 59, 61, 62, 65, 67, 73, 82b, and 102, according to the Kabat numbering, is substituted with another amino acid. In one embodiment, the light chain variable domain of the second antigen-binding site of the present invention is a light chain variable domain in which at least one amino acid residue selected from amino acid residues at positions 3, 8, 15, 24, 26, 27, 29, 30, 31, 32, 38, 48, 49, 50, 79, 92, 94, 95, 95a, and 96 according to the Kabat numbering in the light chain variable domain of the second antigen-binding site is substituted with another amino acid or deleted.

[0071] In one embodiment, a multispecific antigen-binding molecule of the invention comprises: at least one amino acid residue selected from amino acid residues at positions 31, 34, 39, 97, 98, 100, 100a, 100b, and 100e according to the Kabat numbering in the heavy chain variable domain of the first antigen-binding site is substituted with another amino acid or deleted; in the light chain variable domain of the first antigen-binding site, at least one amino acid residue selected from amino acid residues at positions 26, 27, 30, 31, 32, 38, 45, 53, 55, 60, 70, 76, 79, 80, 83, 85, 92, 93, 95, and 96 according to the Kabat numbering is substituted or inserted with another amino acid, in the heavy chain variable domain of the second antigen-binding site, at least one amino acid residue selected from amino acid residues at positions 28, 31, 39, 51, 56, 57, 59, 61, 62, 65, 67, 73, 82b, and 102 according to the Kabat numbering is substituted with another amino acid; in the light chain variable domain of the second antigen-binding site, at least one amino acid residue selected from amino acid residues at positions 3, 8, 15, 24, 26, 27, 29, 30, 31, 32, 38, 48, 49, 50, 79, 92, 94, 95, 95a, and 96 according to the Kabat numbering is substituted with another amino acid or deleted; It is a multispecific antigen-binding molecule.

[0072] In one embodiment, the heavy chain variable domain of the first antigen-binding site of the present invention is a heavy chain variable domain in which the amino acid residue at position 31, according to the Kabat numbering, is histidine, the amino acid residue at position 34 is alanine, the amino acid residue at position 39 is glutamic acid, the amino acid residue at position 97 is aspartic acid, the amino acid residue at position 98 is serine, the amino acid residue at position 100 is aspartic acid or glutamic acid, the amino acid residue at position 100a is aspartic acid or deleted, the amino acid residue at position 100b is alanine or histidine, or the amino acid residue at position 100e is histidine or isoleucine. In one embodiment, the light chain variable domain of the first antigen-binding site of the present invention has, according to the Kabat numbering, an amino acid residue at position 26, an amino acid residue at position 27, an amino acid residue at position 30, an amino acid residue at position 31, an amino acid residue at position 32, an amino acid residue at position 38, an amino acid residue at position 45, an amino acid residue at position 53, an amino acid residue at position 55, an amino acid residue at position 60, an amino acid residue at position 61, an amino acid residue at position 62, an amino acid residue at position 63, an amino acid residue at position 64, an amino acid residue at position 65, an amino acid residue at position 66, an amino acid residue at position 67, an amino acid residue at position 68, an amino acid residue at position 69, an amino acid residue at position 70, an amino acid residue at position 71, an amino acid residue at position 72, an amino acid residue at position 73, an amino acid residue at position 74, an amino acid residue at position 75, an amino acid residue at position 76, an amino acid residue at position 77, an amino acid residue at position 78, an amino acid residue at position 79, an amino acid residue at position 80, an amino acid residue at position 81, an amino acid residue at position 82, an amino acid residue at position 83, an amino acid residue at position 84, an amino acid residue at position 85, an amino acid residue at position 86, an amino acid residue at position 87, an amino acid residue at position 88, an amino acid residue at position 89, an amino acid residue at position 90, an amino acid residue at position 91, an amino acid residue at position 92, an amino acid residue at position 93, an amino acid residue at position 94, an amino acid residue at position 95, an amino acid residue at position 96, an amino acid residue at position 97, an amino acid residue at position 98, an the amino acid residue at position 92 is arginine, the amino acid residue at position 93 is serine or aspartic acid, the amino acid residue at position 95 is proline, or the amino acid residue at position 96 is glycine. In one embodiment, the heavy chain variable domain of the second antigen-binding site of the present invention is a heavy chain variable domain in which the amino acid residue at position 28 is glutamic acid, the amino acid residue at position 31 is asparagine, glutamine or histidine, the amino acid residue at position 39 is lysine, the amino acid residue at position 51 is serine, the amino acid residue at position 56 is threonine or arginine, the amino acid residue at position 57 is valine, the amino acid residue at position 59 is serine, the amino acid residue at position 61 is arginine, the amino acid residue at position 62 is lysine, the amino acid residue at position 65 is asparagine or glutamine, the amino acid residue at position 67 is leucine, the amino acid residue at position 73 is isoleucine, the amino acid residue at position 82b is glutamic acid, or the amino acid residue at position 102 is valine, according to the Kabat numbering. In one embodiment, the light chain variable domain of the second antigen-binding site of the present invention has, in the light chain variable domain of the second antigen-binding site, the amino acid residue at position 3 according to the Kabat numbering is glutamic acid, the amino acid residue at position 8 is proline, the amino acid residue at position 15 is leucine, the amino acid residue at position 24 is threonine, the amino acid residue at position 26 is glutamic acid, the amino acid residue at position 27 is glutamine, the amino acid residue at position 29 is serine, the amino acid residue at position 30 is glutamine, serine or glutamic acid, and the amino acid residue at position 31 is arginine. the amino acid residue at position 32 is glutamine or glutamic acid, the amino acid residue at position 38 is glutamic acid, the amino acid residue at position 48 is isoleucine, the amino acid residue at position 49 is tyrosine, the amino acid residue at position 50 is glutamine, the amino acid residue at position 79 is glutamic acid, the amino acid residue at position 92 is alanine, the amino acid residue at position 94 is aspartic acid, the amino acid residue at position 95 is aspartic acid or alanine, the amino acid residue at position 95a is tyrosine or deleted, or the amino acid residue at position 96 is threonine.

[0073] In one embodiment, a multispecific antigen-binding molecule of the invention comprises: in the heavy chain variable domain of the first antigen-binding site, the amino acid residue at position 31 according to the Kabat numbering is histidine, the amino acid residue at position 34 is alanine, the amino acid residue at position 39 is glutamic acid, the amino acid residue at position 97 is aspartic acid, the amino acid residue at position 98 is serine, the amino acid residue at position 100 is aspartic acid or glutamic acid, the amino acid residue at position 100a is aspartic acid or a deletion, the amino acid residue at position 100b is alanine or histidine, or the amino acid residue at position 100e is histidine or isoleucine; In the light chain variable domain of the first antigen-binding site, the amino acid residue at position 26 according to the Kabat numbering is threonine, the amino acid residue at position 27 is arginine, the amino acid residue at position 30 is arginine, the amino acid residue at position 31 is arginine, the amino acid residue at position 32 is aspartic acid or glutamic acid, the amino acid residue at position 38 is lysine, the amino acid residue at position 45 is glutamic acid, the amino acid residue at position 53 is arginine, the amino acid residue at position 55 is glutamic acid, and the amino acid residue at position 60 is lysine. the amino acid residue at position 70 is aspartic acid, the amino acid residue at position 76 is asparagine, the amino acid residue at position 79 is glutamic acid, the amino acid residue at position 80 is proline or alanine, the amino acid residue at position 83 is methionine or alanine, the amino acid residue at position 85 is threonine, the amino acid residue at position 92 is arginine, the amino acid residue at position 93 is serine or aspartic acid, the amino acid residue at position 95 is proline, or the amino acid residue at position 96 is glycine; in the heavy chain variable domain of the second antigen-binding site, the amino acid residue at position 28 according to the Kabat numbering is glutamic acid, the amino acid residue at position 31 is asparagine, glutamine or histidine, the amino acid residue at position 39 is lysine, the amino acid residue at position 51 is serine, the amino acid residue at position 56 is threonine or arginine, the amino acid residue at position 57 is valine, the amino acid residue at position 59 is serine, the amino acid residue at position 61 is arginine, the amino acid residue at position 62 is lysine, the amino acid residue at position 65 is asparagine or glutamine, the amino acid residue at position 67 is leucine, the amino acid residue at position 73 is isoleucine, the amino acid residue at position 82b is glutamic acid, or the amino acid residue at position 102 is valine; In the light chain variable domain of the second antigen-binding site, the amino acid residue at position 3 according to the Kabat numbering is glutamic acid, the amino acid residue at position 8 is proline, the amino acid residue at position 15 is leucine, the amino acid residue at position 24 is threonine, the amino acid residue at position 26 is glutamic acid, the amino acid residue at position 27 is glutamine, the amino acid residue at position 29 is serine, the amino acid residue at position 30 is glutamine, serine or glutamic acid, the amino acid residue at position 31 is arginine, and the amino acid residue at position 32 is the amino acid residue at position 38 is glutamic acid, the amino acid residue at position 48 is isoleucine, the amino acid residue at position 49 is tyrosine, the amino acid residue at position 50 is glutamine, the amino acid residue at position 79 is glutamic acid, the amino acid residue at position 92 is alanine, the amino acid residue at position 94 is aspartic acid, the amino acid residue at position 95 is aspartic acid or alanine, the amino acid residue at position 95a is tyrosine or deleted, or the amino acid residue at position 96 is threonine; Multispecific antigen binding molecules.

[0074] In one embodiment, the heavy chain variable domain of the first antigen-binding site of the multispecific antigen-binding molecule of the invention is a heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO:56, SEQ ID NO:57, SEQ ID NO:58, SEQ ID NO:59, or SEQ ID NO:60. In one embodiment, the light chain variable domain of the first antigen-binding site of the multispecific antigen-binding molecule of the invention is a light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO:61, SEQ ID NO:62, SEQ ID NO:63, SEQ ID NO:64, SEQ ID NO:65, SEQ ID NO:66, SEQ ID NO:67, SEQ ID NO:68, SEQ ID NO:69, SEQ ID NO:70, SEQ ID NO:71 or SEQ ID NO:72. In one embodiment, the heavy chain variable domain of the second antigen-binding site of the multispecific antigen-binding molecule of the invention is a heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO:73, SEQ ID NO:74, SEQ ID NO:75, SEQ ID NO:76, SEQ ID NO:77, SEQ ID NO:78, SEQ ID NO:79, SEQ ID NO:80, SEQ ID NO:81, SEQ ID NO:82 or SEQ ID NO:83. In one embodiment, the light chain variable domain of the second antigen-binding site of the multispecific antigen-binding molecule of the invention is a light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO:84, SEQ ID NO:85, SEQ ID NO:86, SEQ ID NO:87, SEQ ID NO:88, SEQ ID NO:89, SEQ ID NO:90, SEQ ID NO:91, SEQ ID NO:92, SEQ ID NO:93 or SEQ ID NO:94.

[0075] In one embodiment, the multispecific antigen-binding molecule of the invention comprises the first antigen-binding site: A heavy chain variable domain (QH) of SEQ ID NO: 56, SEQ ID NO: 57, SEQ ID NO: 58, SEQ ID NO: 59, or SEQ ID NO: 60; comprising a light chain variable domain (QL) of SEQ ID NO:61, SEQ ID NO:62, SEQ ID NO:63, SEQ ID NO:64, SEQ ID NO:65, SEQ ID NO:66, SEQ ID NO:67, SEQ ID NO:68, SEQ ID NO:69, SEQ ID NO:70, SEQ ID NO:71 or SEQ ID NO:72, and the second antigen-binding site being A heavy chain variable domain (JH) of SEQ ID NO:73, SEQ ID NO:74, SEQ ID NO:75, SEQ ID NO:76, SEQ ID NO:77, SEQ ID NO:78, SEQ ID NO:79, SEQ ID NO:80, SEQ ID NO:81, SEQ ID NO:82 or SEQ ID NO:83; comprising a light chain variable domain (JL) of SEQ ID NO:84, SEQ ID NO:85, SEQ ID NO:86, SEQ ID NO:87, SEQ ID NO:88, SEQ ID NO:89, SEQ ID NO:90, SEQ ID NO:91, SEQ ID NO:92, SEQ ID NO:93 or SEQ ID NO:94; It is a multispecific antigen-binding molecule.

[0076] In one embodiment, the present invention provides a multispecific antigen-binding molecule comprising a first antibody heavy chain variable domain and an antibody light chain variable domain that bind to FIX and / or FIXa, and a second antibody heavy chain variable domain and an antibody light chain variable domain that bind to FX, the multispecific antigen-binding molecule being described in any of (a) to (v) below. (a) a multispecific antigen-binding molecule comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 56, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 61, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 73, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 84. (QH01 / QL21 / / JH01 / JL01); (b) a multispecific antigen-binding molecule comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 57, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 62, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 73, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 84 (QH02 / QL22 / / JH01 / JL01); (c) a multispecific antigen-binding molecule comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 58, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 63, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 74, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 85 (QH03 / QL23 / / JH02 / JL02); (d) a multispecific antigen-binding molecule comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 58, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 64, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 74, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 85 (QH03 / QL24 / / JH02 / JL02); (e) a multispecific antigen-binding molecule comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 57, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 62, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 75, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 86 (QH02 / QL22 / / JH03 / JL03); (f) a multispecific antigen-binding molecule comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 57, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 62, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 76, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 87 (QH02 / QL22 / / JH04 / JL04); (g) a multispecific antigen-binding molecule comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 57, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 62, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 74, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 85 (QH02 / QL22 / / JH02 / JL02); (h) a multispecific antigen-binding molecule comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 59, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 65, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 74, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 85 (QH04 / QL25 / / JH02 / JL02); (i) a multispecific antigen-binding molecule comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 59, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 66, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 74, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 85 (QH04 / QL26 / / JH02 / JL02); (j) a multispecific antigen-binding molecule comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 59, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 66, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 77, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 88 (QH04 / QL26 / / JH05 / JL05); (k) a multispecific antigen-binding molecule comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 59, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 67, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 77, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 88 (QH04 / QL28 / / JH05 / JL05); (l) a multispecific antigen-binding molecule comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 59, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 67, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 78, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 89 (QH04 / QL28 / / JH06 / JL06); (m) a multispecific antigen-binding molecule comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 59, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 68, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 77, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 88 (QH04 / QL29 / / JH05 / JL05); (n) a multispecific antigen-binding molecule comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 59, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 68, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 78, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 89 (QH04 / QL29 / / JH06 / JL06); (o) a multispecific antigen-binding molecule comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 60, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 69, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 79, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 90 (QH06 / QL30 / / JH07 / JL07); (p) a multispecific antigen-binding molecule comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 59, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 70, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 80, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 91 (QH04 / QL31 / / JH08 / JL08); (q) a multispecific antigen-binding molecule comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 60, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 71, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 79, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 90 (QH06 / QL32 / / JH07 / JL07); (r) a multispecific antigen-binding molecule comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 60, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 71, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 81, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 92 (QH06 / QL32 / / JH09 / JL09); (s) a multispecific antigen-binding molecule comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 60, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 69, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 82, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 93 (QH06 / QL30 / / JH10 / JL10); (t) a multispecific antigen-binding molecule comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 105, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 72, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 83, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 94 (QH07 / QL33 / / JH11 / JL11); (u) a multispecific antigen-binding molecule that binds to the same epitope in both FIX and / or FIXa and the same epitope in FX as that recognized by any one of the antibodies (a) to (t); (v) A multispecific antigen-binding molecule that competes for binding to an epitope in FIX and / or FIXa recognized by any of the antibodies (a) to (t) and an epitope in FX, respectively.

[0077] In one embodiment, the heavy chain constant region of the multispecific antigen binding molecule of the present invention is a heavy chain constant region comprising the amino acid sequence set forth in SEQ ID NO:118, or SEQ ID NO:119. In one embodiment, the light chain constant region of the multispecific antigen binding molecule of the invention is a light chain constant region comprising the amino acid sequence set forth in SEQ ID NO: 100, or SEQ ID NO: 102.

[0078] In one embodiment, the multispecific antigen-binding molecule of the present invention is a multispecific antigen-binding molecule, in which the first antigen-binding site comprises a constant region of the following (1) or (2), and the second antigen-binding site comprises a constant region of the following (1) or (2) that is different from the constant region contained in the first antigen-binding site: (1) SEQ ID NO: 119 as the heavy chain constant region and SEQ ID NO: 100 as the light chain constant region (2) SEQ ID NO: 118 as the heavy chain constant region and SEQ ID NO: 102 as the light chain constant region

[0079] In one embodiment, the multispecific antigen-binding molecule of the present invention is a multispecific antibody. In another embodiment, the multispecific antibody of the present invention is a bispecific antibody.

[0080] In one embodiment, the present invention provides a bispecific antibody comprising a first antibody heavy chain variable domain and an antibody light chain variable domain that bind to FIX and / or FIXa, and a second antibody heavy chain variable domain and an antibody light chain variable domain that bind to FX, the bispecific antibody being described in any of (a) to (v) below: (a) a bispecific antibody comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 56, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 61, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 73, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 84. (QH01 / QL21 / / JH01 / JL01); (b) a bispecific antibody comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 57, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 62, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 73, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 84. (QH02 / QL22 / / JH01 / JL01); (c) a bispecific antibody comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 58, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 63, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 74, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 85. (QH03 / QL23 / / JH02 / JL02); (d) a bispecific antibody comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 58, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 64, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 74, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 85. (QH03 / QL24 / / JH02 / JL02); (e) a bispecific antibody comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 57, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 62, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 75, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 86. (QH02 / QL22 / / JH03 / JL03); (f) a bispecific antibody comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 57, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 62, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 76, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 87. (QH02 / QL22 / / JH04 / JL04); (g) a bispecific antibody comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 57, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 62, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 74, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 85. (QH02 / QL22 / / JH02 / JL02); (h) a bispecific antibody comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 59, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 65, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 74, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 85. (QH04 / QL25 / / JH02 / JL02); (i) a bispecific antibody comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 59, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 66, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 74, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 85. (QH04 / QL26 / / JH02 / JL02); (j) a bispecific antibody comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 59, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 66, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 77, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 88. (QH04 / QL26 / / JH05 / JL05); (k) a bispecific antibody comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 59, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 67, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 77, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 88. (QH04 / QL28 / / JH05 / JL05); (l) a bispecific antibody comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 59, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 67, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 78, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 89. (QH04 / QL28 / / JH06 / JL06); (m) a bispecific antibody comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 59, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 68, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 77, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 88. (QH04 / QL29 / / JH05 / JL05); (n) a bispecific antibody comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 59, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 68, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 78, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 89. (QH04 / QL29 / / JH06 / JL06); (o) a bispecific antibody comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 60, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 69, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 79, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 90. (QH06 / QL30 / / JH07 / JL07); (p) a bispecific antibody comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 59, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 70, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 80, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 91. (QH04 / QL31 / / JH08 / JL08); (q) a bispecific antibody comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 60, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 71, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 79, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 90. (QH06 / QL32 / / JH07 / JL07); (r) a bispecific antibody comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 60, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 71, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 81, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 92. (QH06 / QL32 / / JH09 / JL09); (s) a bispecific antibody comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 60, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 69, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 82, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 93. (QH06 / QL30 / / JH10 / JL10); (t) a bispecific antibody comprising a first antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 105, a first antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 72, a second antibody heavy chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 83, and a second antibody light chain variable domain comprising the amino acid sequence set forth in SEQ ID NO: 94. (QH07 / QL33 / / JH11 / JL11); (u) a bispecific antibody that binds to the same epitope in both blood coagulation factor IX and / or activated blood coagulation factor IX and the same epitope in blood coagulation factor X as those recognized by any of the antibodies (a) to (t); (v) A bispecific antibody that competes for binding to an epitope in blood coagulation factor IX and / or activated blood coagulation factor IX recognized by any of the antibodies (a) to (t) and an epitope in blood coagulation factor X, respectively.

[0081] In one embodiment, the present invention provides a bispecific antibody comprising a first antibody heavy chain and an antibody light chain that bind to FIX and / or FIXa, and a second antibody heavy chain and an antibody light chain that bind to FX, the bispecific antibody being described in any of (a) to (v) below. (a) a bispecific antibody comprising a first antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 120, a first antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 126, a second antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 138, and a second antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 149 (QH01 / QL21 / / JH01 / JL01); (b) a bispecific antibody comprising a first antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 121, a first antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 127, a second antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 138, and a second antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 149 (QH02 / QL22 / / JH01 / JL01); (c) a bispecific antibody comprising a first antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 122, a first antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 128, a second antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 139, and a second antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 150 (QH03 / QL23 / / JH02 / JL02); (d) a bispecific antibody comprising a first antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 122, a first antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 129, a second antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 139, and a second antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 150 (QH03 / QL24 / / JH02 / JL02); (e) a bispecific antibody comprising a first antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 121, a first antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 127, a second antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 140, and a second antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 151 (QH02 / QL22 / / JH03 / JL03); (f) a bispecific antibody comprising a first antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 121, a first antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 127, a second antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 141, and a second antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 152 (QH02 / QL22 / / JH04 / JL04); (g) a bispecific antibody comprising a first antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 121, a first antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 127, a second antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 139, and a second antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 150 (QH02 / QL22 / / JH02 / JL02); (h) a bispecific antibody comprising a first antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 123, a first antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 130, a second antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 139, and a second antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 150 (QH04 / QL25 / / JH02 / JL02); (i) a bispecific antibody comprising a first antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 123, a first antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 131, a second antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 139, and a second antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 150 (QH04 / QL26 / / JH02 / JL02); (j) a bispecific antibody comprising a first antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 123, a first antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 131, a second antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 142, and a second antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 153 (QH04 / QL26 / / JH05 / JL05); (k) a bispecific antibody comprising a first antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 123, a first antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 132, a second antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 142, and a second antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 153 (QH04 / QL28 / / JH05 / JL05); (l) a bispecific antibody comprising a first antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 123, a first antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 132, a second antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 143, and a second antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 154 (QH04 / QL28 / / JH06 / JL06); (m) a bispecific antibody comprising a first antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 123, a first antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 133, a second antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 142, and a second antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 153 (QH04 / QL29 / / JH05 / JL05); (n) a bispecific antibody comprising a first antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 123, a first antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 133, a second antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 143, and a second antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 154 (QH04 / QL29 / / JH06 / JL06); (o) a bispecific antibody comprising a first antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 124, a first antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 134, a second antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 144, and a second antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 155 (QH06 / QL30 / / JH07 / JL07); (p) a bispecific antibody comprising a first antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 123, a first antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 135, a second antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 145, and a second antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 156 (QH04 / QL31 / / JH08 / JL08); (q) a bispecific antibody comprising a first antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 124, a first antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 136, a second antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 144, and a second antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 155 (QH06 / QL32 / / JH07 / JL07); (r) a bispecific antibody comprising a first antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 124, a first antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 136, a second antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 146, and a second antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 157 (QH06 / QL32 / / JH09 / JL09); (s) a bispecific antibody comprising a first antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 124, a first antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 134, a second antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 147, and a second antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 158 (QH06 / QL30 / / JH10 / JL10); (t) a bispecific antibody comprising a first antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 125, a first antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 137, a second antibody heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 148, and a second antibody light chain comprising the amino acid sequence set forth in SEQ ID NO: 159 (QH07 / QL33 / / JH11 / JL11); (u) a bispecific antibody that binds to the same epitope in both FIX and / or FIXa and the same epitope in FX as that recognized by any of the antibodies (a) to (t); (v) A bispecific antibody that competes for binding to an epitope in FIX and / or FIXa recognized by any of the antibodies (a) to (t) and an epitope in FX, respectively.

[0082] In one embodiment, the present invention provides a multispecific antigen-binding molecule having a function substituting for a function of blood coagulation factor VIII, comprising a first antigen-binding site that binds to blood coagulation factor IX and / or activated blood coagulation factor IX, and a second antigen-binding site that binds to blood coagulation factor X, the first antigen-binding site comprises a heavy chain variable domain and a light chain variable domain, the heavy chain variable domain (Q499) comprises HVR-H1 comprising the amino acid sequence of SEQ ID NO: 1, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 2, and HVR-H3 comprising the amino acid sequence of SEQ ID NO: 3; The light chain variable domain (QNK131) comprises HVR-L1 comprising the amino acid sequence of SEQ ID NO: 162, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 163, and HVR-L3 comprising the amino acid sequence of SEQ ID NO: 164; and the second antigen-binding site comprises a heavy chain variable domain and a light chain variable domain, the heavy chain variable domain (J327) comprises HVR-H1 comprising the amino acid sequence of SEQ ID NO: 4, HVR-H2 comprising the amino acid sequence of SEQ ID NO: 5, and HVR-H3 comprising the amino acid sequence of SEQ ID NO: 6; The multispecific antigen-binding molecule also provides the above-mentioned, wherein the light chain variable domain (JNL095) comprises HVR-L1 comprising the amino acid sequence of SEQ ID NO: 165, HVR-L2 comprising the amino acid sequence of SEQ ID NO: 166, and HVR-L3 comprising the amino acid sequence of SEQ ID NO: 167.

[0083] In a specific embodiment, the antibodies (a) to (v) above are antibodies having a function that substitutes for the function of FVIII. In another embodiment, the antibodies (a) to (v) are antibodies whose reactivity with anti-ACE910 (Emicizumab) idiotype antibodies is reduced compared to ACE910 (Emicizumab). Here, "whose reactivity with anti-ACE910 (Emicizumab) idiotype antibodies is reduced compared to ACE910 (Emicizumab)" means that, when the binding strength (%) between the anti-idiotype antibody and labeled ACE910 is measured using the method described herein, the binding strength is reduced by preferably 10% or more, more preferably 20% or more, and even more preferably 30% or more when a test substance is added at a concentration of 100 μg / mL. The anti-ACE910 idiotype antibody can be obtained, for example, by the following method. 2 and F(ab') of J327 / L404, which recognizes FX. 2 was administered to rabbits, and anti-Q499 / L404 F(ab') 2 Rabbit serum and anti-J327 / L404 F(ab') 2 Rabbit serum is obtained. The ammonium sulfate fraction of this serum is passed through a human IgG binding column to remove human IgG reactive antibodies. Next, affinity purification is performed using a Q499 / L404 (or J327 / L404) binding column, and then a process of removing J327 / L404 (or Q499 / L404) reactive antibodies is performed using a J327 / L404 (or Q499 / L404) binding column to obtain polyclonal anti-idiotypic antibodies that specifically bind to Q499 / L404 (or J327 / L404). The binding strength of ACE910 (Emicizumab) labeled with an anti-idiotype antibody can be measured, for example, by electrochemiluminescence immunoassay, which allows the binding inhibition by the test substance to be evaluated. When using the anti-idiotype antibody obtained above, the test substance (bispecific antibody) is added to a mixture of anti-Q499 / L404 idiotype antibody, anti-J327 / L404 idiotype antibody, biotin-labeled ACE910 (Emicizumab) and SULFO-TAG-labeled ACE910 (Emicizumab) at a concentration of 0, 1, 3, 10, 30 or 100 μg / mL, and the resulting mixture is incubated overnight under refrigeration. Next, the mixture is added to a 96-well plate, the plate is washed, and the binding strength is measured by electrochemiluminescence. In another embodiment, the antibodies (a) to (v) have enhanced FVIII cofactor functional substituting activity compared to ACE910 (Emicizumab), and have high activity even at a low antibody concentration. In another embodiment, the antibodies (a) to (v) have substantially no FIX activation inhibitory activity and have increased FVIII cofactor functional substituting activity compared to ACE910 (Emicizumab). Here, "having substantially no FIX activation inhibitory activity" means that, when the OD value is measured using the method described herein, the decrease in OD value compared to the OD value of the control is within 0.025, preferably within 0.02, and more preferably within 0.01.

[0084] In addition, the amino acids contained in the amino acid sequences described in the present invention may be modified after translation (for example, modification of N-terminal glutamine to pyroglutamic acid by pyroglutamylation is a modification well known to those skilled in the art), but even if an amino acid is modified after translation in this way, it is naturally included in the amino acid sequences described in the present invention.

[0085] In a further aspect, the present invention provides a multispecific antigen-binding molecule or bispecific antibody that binds to the same epitope as the multispecific antigen-binding molecule or bispecific antibody that binds to FIX and / or FIXa and FX provided herein. For example, in a specific embodiment, a multispecific antigen-binding molecule or bispecific antibody that binds to the same epitope as the multispecific antigen-binding molecule or bispecific antibody described in (a) to (t) above is provided.

[0086] In a further aspect of the invention, the bispecific antibody that binds FIX and / or FIXa and FX according to any of the above embodiments is a monoclonal antibody, including a chimeric, humanized or human antibody. In one embodiment, the bispecific antibody that binds FIX and / or FIXa and FX is, for example, a (scFv) 2 , diabody, or F(ab') 2 In another embodiment, the antibody is an antibody fragment, such as, for example, Complete IgG4 antibody or any other antibody class or isotype as defined herein.

[0087] In a further aspect, a bispecific antibody binding to FIX and / or FIXa and FX according to any of the above embodiments may incorporate, alone or in combination, any of the features described in items 1 to 7 below.

[0088] 1. Antibody affinity In certain embodiments, the antibodies provided herein have a cytotoxicity of ≦100 μM, ≦10 μM, ≦1 μM, ≦100 nM, ≦10 nM, ≦1 nM, ≦0.1 nM, ≦0.01 nM, or ≦0.001 nM (e.g., 10 -5 M or less, e.g. 10 -5 M~10 -10 M, for example 10 -6 M~10 -10 It has a dissociation constant (Kd) of 1 M.

[0089] In one embodiment, Kd is measured by radiolabeled antigen binding assay (RIA). In one embodiment, the RIA is performed using a Fab version of the antibody of interest and its antigen. For example, the solution binding affinity of the Fab for the antigen is determined by measuring the binding affinity of the Fab to the antigen at the lowest concentration ( 125 I) Fab is equilibrated with labeled antigen, and then bound antigen is captured by a plate coated with anti-Fab antibody. (See, e.g., Chen et al., J. Mol. Biol. 293:865-881(1999)). To establish the measurement conditions, MICROTITER® multi-well plates (Thermo Scientific) are coated overnight with 5 μg / ml of capture anti-Fab antibody (Cappel Labs) in 50 mM sodium carbonate (pH 9.6), and then blocked with 2% (w / v) bovine serum albumin in PBS for 2-5 hours at room temperature (approximately 23°C). In non-adsorbent plates (Nunc #269620), 100 pM or 26 pM of [ 125 I]-antigen is mixed with serial dilutions of the Fab of interest (e.g., as in the evaluation of the anti-VEGF antibody, Fab-12, in Presta et al., Cancer Res. 57:4593-4599 (1997)). The Fab of interest is then incubated overnight, although the incubation can be continued for longer (e.g., about 65 hours) to ensure that equilibrium is reached. The mixture is then transferred to a capture plate for incubation at room temperature (e.g., 1 hour). The solution is then removed and the plate is washed 8 times with 0.1% polysorbate 20 (TWEEN-20®) in PBS. Once the plate has dried, 150 μl / well of scintillant (MICROSCINT-20™, Packard) is added and the plate is counted for 10 minutes in a TOPCOUNT™ gamma counter (Packard). The concentration of each Fab that gives 20% or less of maximum binding is selected for use in the competitive binding assay.

[0090] In another embodiment, Kd is measured using a BIACORE® surface plasmon resonance assay. For example, measurements using a BIACORE®-2000 or BIACORE®-3000 (BIAcore, Inc., Piscataway, NJ) are performed at 25° C. using a CM5 chip with approximately 10 response units (RU) of antigen immobilized. In one embodiment, a carboxymethylated dextran biosensor chip (CM5, BIACORE, Inc.) is activated with N-ethyl-N'-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) according to the supplier's instructions. The antigen is diluted to 5 μg / ml (approximately 0.2 μM) with 10 mM sodium acetate, pH 4.8, before being injected at a flow rate of 5 μl / min to achieve approximately 10 response units (RU) of protein binding. After injection of antigen, 1 M ethanolamine is injected to block unreacted groups. For kinetic measurements, two-fold serial dilutions of Fab (0.78 nM to 500 nM) are injected in PBS containing 0.05% polysorbate 20 (TWEEN-20™) surfactant (PBST) at 25° C. with a flow rate of approximately 25 μl / min. The binding rate (k on ) and dissociation rate (k off ) is calculated by simultaneously fitting the association and dissociation sensorgrams with a simple one-to-one Langmuir binding model (BIACORE® Evaluation Software version 3.2). The equilibrium dissociation constant (Kd) is k off / k on The on-rate is calculated as a ratio of 10 to 10 by the surface plasmon resonance assay described above. 6 M -1 s -1If the on-rate exceeds , the on-rate can be determined by using a fluorescence quenching technique to measure the increase or decrease in fluorescence emission intensity (excitation = 295 nm; emission = 340 nm, band pass 16 nm) of 20 nM anti-antigen antibody (Fab form) in PBS, pH 7.2 at 25°C in the presence of increasing concentrations of antigen as measured in a spectrometer (e.g., a stopped-flow spectrophotometer (Aviv Instruments) or an 8000 series SLM-AMINCO™ spectrophotometer (ThermoSpectronic) using a stirred cuvette).

[0091] 2. Antibody fragment In certain embodiments, the antibodies provided herein are antibody fragments. Antibody fragments include, but are not limited to, (scFv) 2 , diabody, or F(ab') 2 Antibody fragments include F(ab') fragments, as well as other fragments described below. For a review of specific antibody fragments, see Hudson et al. Nat. Med. 9:129-134 (2003). For a review of scFv fragments, see, e.g., Pluckthun, in The Pharmacology of Monoclonal Antibodies, vol. 113, Rosenburg and Moore eds., (Springer-Verlag, New York), pp.269-315 (1994); in addition, WO93 / 16185; and U.S. Patent Nos. 5,571,894 and 5,587,458. F(ab') fragments that contain salvage receptor binding epitope residues and have extended in vivo half-lives are also known. 2 For a discussion of fragments, see US Pat. No. 5,869,046.

[0092] Diabodies are antibody fragments with two antigen-binding sites that may be bivalent or bispecific. See, e.g., EP 404,097; WO 1993 / 01161; Hudson et al., Nat. Med. 9:129-134 (2003); Hollinger et al., Proc. Natl. Acad. Sci. USA 90: 6444-6448 (1993).

[0093] Antibody fragments can be produced by a variety of techniques, including, but not limited to, proteolytic digestion of whole antibodies and production by recombinant host cells (e.g., E. coli or phages), as described herein.

[0094] 3. Chimeric and humanized antibodies In certain embodiments, the antibody provided herein is a chimeric antibody. Certain chimeric antibodies are described, for example, in U.S. Patent No. 4,816,567; and Morrison et al., Proc. Natl. Acad. Sci. USA, 81:6851-6855 (1984). In one example, a chimeric antibody comprises a non-human variable region (e.g., a variable region derived from a non-human primate, such as a mouse, rat, hamster, rabbit, or monkey) and a human constant region. In a further example, a chimeric antibody is a "class-switched" antibody whose class or subclass has been changed from that of the parent antibody. Chimeric antibodies also include antigen-binding fragments thereof.

[0095] In certain embodiments, the chimeric antibody is a humanized antibody. Typically, a non-human antibody is humanized to reduce immunogenicity in humans while maintaining the specificity and affinity of the parent non-human antibody. Usually, a humanized antibody comprises one or more variable domains, in which the HVRs (e.g., CDRs (or portions thereof)) are derived from a non-human antibody and the FRs (or portions thereof) are derived from human antibody sequences. The humanized antibody optionally comprises at least a portion of a human constant region. In some embodiments, some FR residues in the humanized antibody are replaced with corresponding residues from a non-human antibody (e.g., the antibody from which the HVR residues are derived), e.g., to restore or improve the specificity or affinity of the antibody.

[0096] Humanized antibodies and methods for making them are reviewed in Almagro and Fransson, Front. Biosci. 13:1619-1633 (2008) and also see, e.g., Riechmann et al., Nature 332:323-329 (1988); Queen et al., Proc. Nat'l Acad. Sci. USA 86:10029-10033 (1989); U.S. Pat. Nos. 5,821,337, 7,527,791, 6,982,321, and 7,087,409; Kashmiri et al., Methods 36:25-34 (2005) (describing specificity determining region (SDR) grafting); Padlan, Mol. Immunol. 28:489-498 (1991) (describing resurfacing); Dall'Acqua et al., Methods 36:43-60 (2005) (describing FR shuffling); and further described in Osbourn et al., Methods 36:61-68 (2005) and Klimka et al., Br. J. Cancer, 83:252-260 (2000) (describing a "guide selection" approach for FR shuffling).

[0097] Human framework regions that may be used for humanization include, but are not limited to: framework regions selected using the "best-fit" method (see Sims et al. J. Immunol. 151:2296 (1993)); framework regions derived from consensus sequences of human antibodies of particular subgroups of light or heavy chain variable regions (see Carter et al. Proc. Natl. Acad. Sci. USA, 89:4285 (1992) and Presta et al. J. Immunol., 151:2623 (1993)); human mature (somatically mutated) framework regions or human germline framework regions (see, e.g., Almagro and Fransson, Front. Biosci. 13:1619-1633 (2008)); and framework regions derived from screening of FR libraries (Baca et al., J. Biol. Chem. 272:10678-10684 (1997)). and Rosok et al., J. Biol. Chem. 271:22611-22618 (1996).

[0098] 4. Human antibodies In certain embodiments, the antibody provided herein is a human antibody. Human antibodies can be produced by various techniques known in the art. Human antibodies are reviewed in van Dijk and van de Winkel, Curr. Opin. Pharmacol. 5: 368-74 (2001) and Lonberg, Curr. Opin. Immunol. 20:450-459 (2008).

[0099] Human antibodies may be prepared by administering an immunogen to a transgenic animal that has been engineered to produce fully human antibodies or complete antibodies with human variable regions in response to antigen challenge. Such animals typically contain all or a portion of a human immunoglobulin locus, which either replaces an endogenous immunoglobulin locus or is present extrachromosomally or randomly integrated into the animal's chromosomes. In such transgenic mice, the endogenous immunoglobulin locus is usually inactivated. For a review of methods for obtaining human antibodies from transgenic animals, see Lonberg, Nat. Biotech. 23:1117-1125 (2005). See also, for example, U.S. Patent Nos. 6,075,181 and 6,150,584, which describe XENOMOUSE™ technology; U.S. Patent No. 5,770,429, which describes HUMAB® technology; U.S. Patent No. 7,041,870, which describes KM MOUSE® technology; and U.S. Patent Application Publication No. 2007 / 0061900, which describes VELOCIMOUSE® technology. The human variable regions from complete antibodies generated by such animals may be further modified, for example, by combining with different human constant regions.

[0100] Human antibodies can also be produced by hybridoma-based methods. Human myeloma and mouse-human heteromyeloma cell lines for the production of human monoclonal antibodies have been described. (See, e.g., Kozbor J. Immunol., 133: 3001 (1984); Brodeur et al., Monoclonal Antibody Production Techniques and Applications, pp.51-63 (Marcel Dekker, Inc., New York, 1987); and Boerner et al., J. Immunol., 147: 86 (1991).) Human antibodies generated via human B-cell hybridoma technology are also described in Li et al., Proc. Natl. Acad. Sci. USA, 103:3557-3562 (2006). Additional methods include those described, for example, in U.S. Patent No. 7,189,826 (describing the production of monoclonal human IgM antibodies from hybridoma cell lines) and Ni, Xiandai Mianyixue, 26(4):265-268 (2006) (describing human-human hybridomas). Human hybridoma technology (trioma technology) is also described in Vollmers and Brandlein, Histology and Histopathology, 20(3):927-937 (2005) and Vollmers and Brandlein, Methods and Findings in Experimental and Clinical Pharmacology, 27(3):185-91 (2005).

[0101] Human antibodies can also be generated by isolating Fv clone variable domain sequences selected from a human-derived phage display library. Such variable domain sequences can then be combined with the desired human constant domains. Techniques for selecting human antibodies from antibody libraries are described below.

[0102] 5. Library-derived Antibodies Antibodies of the invention may be isolated by screening combinatorial libraries for antibodies with the desired activity or activities. For example, various methods are known in the art for generating phage display libraries and screening such libraries for antibodies with the desired binding characteristics. Such methods are reviewed in Hoogenboom et al. in Methods in Molecular Biology 178:1-37 (O'Brien et al., ed., Human Press, Totowa, NJ, 2001) and further described in, e.g., McCafferty et al., Nature 348:552-554;Clackson et al., Nature 352: 624-628 (1991); Marks et al., J. Mol. Biol. 222: 581-597 (1992); Marks and Bradbury, in Methods in Molecular Biology 248:161-175 (Lo, ed., Human Press, Totowa, NJ, 2003); Sidhu et al., J. Mol. Biol. 338(2): 299-310 (2004); Lee et al., J. Mol. Biol. 340(5): 1073-1093 (2004); Fellouse, Proc. Natl. Acad. Sci. USA 101(34):12467-12472 (2004); and Lee et al., J. Immunol. Methods 284(1-2): 119-132(2004).

[0103] In a particular phage display method, repertoires of VH and VL genes are cloned separately by polymerase chain reaction (PCR) and randomly recombined in a phage library, which can be screened for antigen-binding phage as described in Winter et al., Ann. Rev. Immunol., 12: 433-455 (1994). Phages typically display antibody fragments, either as single-chain Fv (scFv) fragments or as Fab fragments. Libraries from immunized sources provide high affinity antibodies to the immunogen without the need to construct hybridomas. Alternatively, naive repertoires can be cloned (e.g., from humans) to provide a single source of antibodies to a wide range of non-self and self antigens without immunization, as described in Griffiths et al., EMBO J, 12: 725-734 (1993). Finally, naive libraries can be generated synthetically by cloning unrearranged V-gene segments from stem cells and using PCR primers that encode the hypervariable CDR3 regions and contain random sequences to achieve rearrangement in vitro, as described in Hoogenboom and Winter, J. Mol. Biol., 227: 381-388 (1992). Patent literature describing human antibody phage libraries includes, for example: U.S. Pat. No. 5,750,373, and U.S. Patent Application Publication Nos. 2005 / 0079574, 2005 / 0119455, 2005 / 0266000, 2007 / 0117126, 2007 / 0160598, 2007 / 0237764, 2007 / 0292936, and 2009 / 0002360.

[0104] Antibodies or antibody fragments isolated from a human antibody library are considered herein to be human antibodies or human antibody fragments.

[0105] 6. Multispecific antibodies In the present invention, the term "multispecific antibody" refers to an antibody capable of specifically binding to a variety of different epitopes. In other words, a multispecific antibody is an antibody having specificity for at least two different epitopes, and includes antibodies that bind to different antigens as well as antibodies that bind to different epitopes on the same antigen. (For example, when the antigen is a heteroreceptor, the multispecific antibody binds to different domains constituting the heteroreceptor, or when the antigen is a monomer, the multispecific antibody binds to multiple sites on the monomeric antigen.) Usually, such molecules bind to two antigens (bispecific antibodies), but they may also have specificity for more than two antigens (e.g., three types). In a specific embodiment, one of the antigens is FIX and / or FIXa, and the other is FX. Bispecific antibodies can be prepared as full-length antibodies or as antibody fragments.

[0106] Techniques for producing multispecific antibodies include, but are not limited to, recombinant co-expression of two immunoglobulin heavy chain-light chain pairs with different specificities (see Milstein and Cuello, Nature 305: 537 (1983), WO93 / 08829, and Traunecker et al., EMBO J. 10: 3655 (1991)), and the knob-in-hole technology (see, e.g., U.S. Pat. No. 5,731,168). Multispecific antibodies can be made by manipulating electrostatic steering effects to create Fc heterodimeric molecules (WO2009 / 089004A1); cross-linking two or more antibodies or fragments (see U.S. Pat. No. 4,676,980 and Brennan et al., Science, 229: 81 (1985)); using leucine zippers to generate antibodies with two specificities (see Kostelny et al., J. Immunol., 148(5):1547-1553 (1992)); using "diabody" technology to create bispecific antibody fragments (see Hollinger et al., Proc. Natl. Acad. Sci. USA, 90:6444-6448 (1993)); and using single-chain Fv (scFv) dimers (Gruber et al., J. Immunol., 152:5368 (1993)). (1994)); and by preparing trispecific antibodies as described, for example, in Tutt et al. J. Immunol. 147: 60 (1991).

[0107] Engineered antibodies with three or more functional antigen-binding sites are also included herein, including "octopus antibodies" (see, eg, US Patent Application Publication No. 2006 / 0025576 A1).

[0108] 7. Antibody Variants In certain embodiments, amino acid sequence variants of the antibodies provided herein are also contemplated. For example, it may be desirable to improve the binding affinity and / or other biological properties of the antibody. Amino acid sequence variants of the antibody may be prepared by introducing appropriate modifications into the nucleotide sequence encoding the antibody or by peptide synthesis. Such modifications include, for example, deletions from, and / or insertions into, and / or substitutions of residues in the amino acid sequence of the antibody. Any combination of deletions, insertions, and substitutions may be made to arrive at the final construct, provided that the final construct possesses the desired characteristics (e.g., antigen binding).

[0109] a) Substitution, insertion, and deletion mutants In certain embodiments, antibody variants are provided that have one or more amino acid substitutions. Target sites for substitutional mutagenesis include HVRs and FRs. Conservative substitutions are shown in Table 1 under the heading of "preferred substitutions". More substantial changes are provided in Table 1 under the heading of "exemplary substitutions" and are detailed below with reference to classes of amino acid side chains. Amino acid substitutions may be introduced into the antibody of interest, and the products may be screened for desired activity, such as, for example, retained / improved antigen binding, reduced immunogenicity, or improved ADCC or CDC.

[0110] [Table 1]

[0111] Amino acids can be divided into groups according to common side chain properties: (1) Hydrophobic: norleucine, methionine (Met), alanine (Ala), valine (Val), leucine (Leu), isoleucine (Ile); (2) neutral hydrophilic: cysteine ​​(Cys), serine (Ser), threonine (Thr), asparagine (Asn), glutamine (Gln); (3) Acidic: aspartic acid (Asp), glutamic acid (Glu); (4) Basic: histidine (His), lysine (Lys), arginine (Arg); (5) Residues that affect chain orientation: glycine (Gly), proline (Pro); (6) Aromatic: tryptophan (Trp), tyrosine (Tyr), phenylalanine (Phe). Non-conservative substitutions refer to the exchange of a member of one of these classes for one from another class.

[0112] One type of substitutional variant involves the substitution of one or more hypervariable region residues of a parent antibody (e.g., a humanized or human antibody). Usually, the resulting variant selected for further study will have a modification (e.g., an improvement) in a particular biological property compared to the parent antibody (e.g., increased affinity, decreased immunogenicity) and / or will substantially retain a particular biological property of the parent antibody. An exemplary substitutional variant is an affinity matured antibody, which may be conveniently generated, for example, using phage display-based affinity maturation techniques (e.g., those described herein). Briefly, one or more HVR residues are mutated, and the mutated antibodies are displayed on phage and screened for a particular biological activity (e.g., binding affinity).

[0113] Modifications (e.g., substitutions) can be made in HVRs, for example, to improve the affinity of the antibody. Such modifications can be made in "hot spots" of HVRs, i.e., residues encoded by codons where mutations occur frequently during the somatic maturation process (see, e.g., Chowdhury, Methods Mol. Biol. 207:179-196 (2008)) and / or residues that contact antigens, and the resulting mutated VH or VL can be tested for binding affinity. Affinity maturation by construction and reselection from secondary libraries is described, for example, in Hoogenboom et al. in Methods in Molecular Biology 178:1-37 (O'Brien et al., ed., Human Press, Totowa, NJ, (2001)). In some embodiments of affinity maturation, diversity is introduced into the variable genes selected for maturation by any of a variety of methods (e.g., error-prone PCR, chain shuffling, or oligonucleotide-directed mutagenesis). A secondary library is then created. This library is then screened to identify any antibody variants with the desired affinity. Another method of introducing diversity involves an HVR-directed approach, where several HVR residues (e.g., 4-6 residues at a time) are randomized. HVR residues involved in antigen binding can be specifically identified, for example, using alanine scanning mutagenesis or modeling. In particular, CDR-H3 and CDR-L3 are often targeted.

[0114] In certain embodiments, substitution, insertion, or deletion may be made within one or more HVRs, as long as such modification does not substantially reduce the ability of the antibody to bind to antigen.For example, conservative modifications (e.g., conservative substitutions as provided herein) that do not substantially reduce binding affinity may be made in HVRs.Such modifications may, for example, be outside the antigen contact residues of HVRs.In certain embodiments of the above mutant VH and VL sequences, each HVR is unmodified or contains only one, two, or three amino acid substitutions.

[0115] A useful method for identifying antibody residues or regions that can be targeted for mutagenesis is that described by Cunningham and Wells (1989) Science, 244:1081-1085, called "alanine scanning mutagenesis". In this method, a residue or group of target residues (e.g., charged residues such as arginine, aspartic acid, histidine, lysine, and glutamic acid) is identified and replaced with neutral or negatively charged amino acids (e.g., alanine or polyalanine), and it is determined whether the antibody-antigen interaction is affected. Further substitutions can be introduced at amino acid positions that show functional sensitivity to this initial substitution. Alternatively or additionally, a crystal structure of the antigen-antibody complex can be analyzed to identify contact points between the antibody and the antigen. Such contact residues and neighboring residues can be targeted as replacement candidates or excluded from replacement candidates. Mutants can be screened to determine whether they contain the desired properties.

[0116] Amino acid sequence insertions include fusions ranging in length from one residue to polypeptides containing 100 or more residues at the amino and / or carboxyl termini, as well as single or multiple amino acid residue insertions within the sequence. An example of a terminal insertion includes an antibody with a methionyl residue at the N-terminus. Other insertional variants of the antibody molecule include the fusion to the N- or C-terminus of the antibody of an enzyme (e.g., for ADEPT) or a polypeptide which increases the plasma half-life of the antibody.

[0117] b) Glycosylation Mutants In certain embodiments, the antibodies provided herein have been modified to increase or decrease the extent to which the antibody is glycosylated. Adding or deleting glycosylation sites to an antibody can be conveniently accomplished by modifying the amino acid sequence to create or remove one or more glycosylation sites.

[0118] If the antibody comprises an Fc region, the carbohydrate attached thereto may be modified. Natural antibodies produced by mammalian cells typically comprise branched, biantennary oligosaccharides, which are usually attached by N-linkage to Asn297 in the CH2 domain of the Fc region. See, e.g., Wright et al. TIBTECH 15:26-32 (1997). Oligosaccharides include, for example, various carbohydrates such as mannose, N-acetylglucosamine (GlcNAc), galactose, and sialic acid, as well as fucose attached to the GlcNAc in the "stem" of the biantennary oligosaccharide structure. In some embodiments, modifications of the oligosaccharides in the antibodies of the invention may be made to generate antibody variants with specific improved properties.

[0119] c) Fc region mutants In certain embodiments, one or more amino acid modifications may be introduced into the Fc region of an antibody provided herein, thereby generating an Fc region variant. The Fc region variant may comprise a human Fc region sequence (e.g., a human IgG1, IgG2, IgG3, or IgG4 Fc region) that contains an amino acid modification (e.g., a substitution) at one or more amino acid positions.

[0120] In certain embodiments, antibody variants with some, but not all, effector functions are also contemplated by the present invention, which make the antibody a desirable candidate for applications where its in vivo half-life is important, but certain effector functions (such as complement and ADCC) are unnecessary or detrimental. In vitro and / or in vivo cytotoxicity assays can be performed to confirm the reduction / lack of CDC and / or ADCC activity. For example, Fc receptor (FcR) binding assays can be performed to confirm that the antibody lacks FcγR binding (and thus likely lacks ADCC activity) while retaining FcRn binding. NK cells, the primary cells mediating ADCC, express only FcγRIII, whereas monocytes express FcγRI, FcγRII, and FcγRIII. Expression of FcR on hematopoietic cells is summarized in Table 3 on page 464 of Ravetch and Kinet, Annu. Rev. Immunol. 9:457-492 (1991). Non-limiting examples of in vitro assays to assess ADCC activity of a molecule of interest are described in U.S. Patent No. 5,500,362 (see, e.g., Hellstrom, I. et al. Proc. Nat'l Acad. Sci. USA 83:7059-7063 (1986)) and Hellstrom, I et al., Proc. Nat'l Acad. Sci. USA 82:1499-1502 (1985); U.S. Patent No. 5,821,337 (see, Bruggemann, M. et al., J. Exp. Med. 166:1351-1361 (1987)). Alternatively, non-radioactive assays may be used (see, e.g., ACT1™ non-radioactive cytotoxicity assay for flow cytometry (CellTechnology, Inc. Mountain View, CA); and CytoTox 96® non-radioactive cytotoxicity assays (Promega, Madison, WI)).Useful effector cells for such assays include peripheral blood mononuclear cells (PBMC) and natural killer (NK) cells. Alternatively or additionally, ADCC activity of the molecule of interest may be assessed in vivo, e.g., in an animal model such as that described in Clynes et al. Proc. Nat'l Acad. Sci. USA 95:652-656 (1998). C1q binding assays may also be performed to confirm that the antibody is unable to bind C1q and thus lacks CDC activity. See, e.g., C1q and C3c binding ELISAs in WO2006 / 029879 and WO2005 / 100402. CDC measurements may also be performed to assess complement activation (see, e.g., Gazzano-Santoro et al., J. Immunol. Methods 202:163 (1996); Cragg, MS et al., Blood 101:1045-1052 (2003); and Cragg, MS and MJ Glennie, Blood 103:2738-2743 (2004)). In addition, FcRn binding and in vivo clearance / half-life determinations may also be performed using methods known in the art (see, e.g., Petkova, SB et al., Int'l. Immunol. 18(12):1759-1769 (2006)).

[0121] Certain antibody variants with increased or decreased binding to FcRs have been described (see U.S. Pat. No. 6,737,056; WO2004 / 056312, and Shields et al., J. Biol. Chem. 9(2): 6591-6604 (2001)).

[0122] Antibodies with increased half-lives and increased binding to the neonatal Fc receptor (FcRn, responsible for the transfer of maternal IgGs to the fetus (Guyer et al., J. Immunol. 117:587 (1976) and Kim et al., J. Immunol. 24:249 (1994)) have been described in U.S. Patent Application Publication No. 2005 / 0014934 A1 (Hinton et al.). These antibodies comprise an Fc region with one or more substitutions therein that increase binding of the Fc region to FcRn. Such Fc variants include those with substitutions at one or more of Fc region residues: 238, 256, 265, 272, 286, 303, 305, 307, 311, 312, 317, 340, 356, 360, 362, 376, 378, 380, 382, ​​413, 424, or 434 (e.g., substitution at Fc region residue 434 (U.S. Patent No. 7,371,826)).

[0123] For other examples of Fc region variants, see also Duncan & Winter, Nature 322:738-40 (1988); U.S. Patent No. 5,648,260; U.S. Patent No. 5,624,821; and WO 94 / 29351.

[0124] d) Cysteine ​​Engineered Antibody Variants In certain embodiments, it may be desirable to create cysteine ​​engineered antibodies (e.g., "thioMAbs") in which one or more residues of an antibody are substituted with a cysteine ​​residue. In certain embodiments, the substituted residues occur at accessible sites of the antibody. By substituting those residues with cysteine, reactive thiol groups are placed at accessible sites of the antibody, which may be used to conjugate the antibody to other moieties (such as drug moieties or linker-drug moieties) to create immunoconjugates as further detailed herein. In certain embodiments, any one or more of the following residues may be substituted with a cysteine: V205 (Kabat numbering) of the light chain; A118 (EU numbering) of the heavy chain; and S400 (EU numbering) of the heavy chain Fc region. Cysteine ​​engineered antibodies may be generated, for example, as described in U.S. Pat. No. 7,521,541.

[0125] e) Antibody derivative In certain embodiments, the antibodies provided herein may be further modified to include additional non-protein moieties that are known in the art and readily available. Moieties suitable for derivatization of antibodies include, but are not limited to, water-soluble polymers. Non-limiting examples of water-soluble polymers include, but are not limited to, polyethylene glycol (PEG), ethylene glycol / propylene glycol copolymers, carboxymethylcellulose, dextran, polyvinyl alcohol, polyvinylpyrrolidone, poly 1,3 dioxolane, poly 1,3,6, trioxane, ethylene / maleic anhydride copolymers, polyamino acids (either homopolymers or random copolymers), and dextran or poly(n-vinylpyrrolidone) polyethylene glycol, polypropylene glycol homopolymer, polypropylene oxide / ethylene oxide copolymer, polyoxyethylated polyols (e.g., glycerol), polyvinyl alcohol, and mixtures thereof. Polyethylene glycol propionaldehyde may be advantageous in manufacturing due to its stability in water. The polymer may be of any molecular weight and may be branched or unbranched. The number of polymers attached to an antibody can vary, and if more than one polymer is attached, they can be the same molecule or different molecules. In general, the number and / or type of polymers used for derivatization can be determined based on considerations such as, but not limited to, the particular property or function of the antibody to be improved, whether the antibody derivative will be used in therapy under defined conditions, etc.

[0126] In another embodiment, a conjugate is provided between an antibody and a non-protein moiety that can be selectively heated by exposure to radiation. In one embodiment, the non-protein moiety is a carbon nanotube (Kam et al., Proc. Natl. Acad. Sci. USA 102: 11600-11605 (2005)). The radiation can be of any wavelength, including but not limited to wavelengths that heat the non-protein moiety to a temperature that is not harmful to normal cells but that kills cells in close proximity to the antibody-non-protein moiety.

[0127] B. Recombinant Methods and Constructs Antibodies can be produced using recombinant methods and constructs, for example as described in U.S. Pat. No. 4,816,567. In one embodiment, an isolated nucleic acid is provided that encodes a multispecific antibody that binds to FIX and / or FIXa and FX as described herein. Such a nucleic acid may encode an amino acid sequence comprising the VL and / or an amino acid sequence comprising the VH of the antibody (e.g., the light and / or heavy chains of the antibody). In a further embodiment, one or more vectors (e.g., expression vectors) comprising such a nucleic acid are provided. In a further embodiment, a host cell comprising such a nucleic acid is provided. In one such embodiment, the host cell comprises (e.g., is transformed with) (1) a vector comprising a nucleic acid encoding an amino acid sequence comprising the VL of the antibody and an amino acid sequence comprising the VH of the antibody, or (2) a first vector comprising a nucleic acid encoding an amino acid sequence comprising the VL of the antibody and a second vector comprising a nucleic acid encoding an amino acid sequence comprising the VH of the antibody. In one embodiment, the host cell is eukaryotic (e.g., a Chinese hamster ovary (CHO) cell) or a lymphoid cell (e.g., a Y0, NS0, Sp2 / 0 cell)). In one embodiment, a method is provided for making a multispecific antibody that binds FIX and / or FIXa and FX, comprising culturing a host cell comprising nucleic acid encoding said antibody as described above under conditions suitable for expression of said multispecific antibody that binds FIX and / or FIXa and FX, and optionally recovering said antibody from the host cell (or host cell culture medium).

[0128] For recombinant production of multispecific antibody molecules that bind to FIX and / or FIXa and FX, nucleic acids encoding the antibodies (such as those described above) are isolated and inserted into one or more vectors for further cloning and / or expression in a host cell. Such nucleic acids may be readily isolated and sequenced using conventional procedures (e.g., using oligonucleotide probes capable of specifically binding to genes encoding the antibody heavy and light chains).

[0129] Suitable host cells for cloning or expressing antibody-encoding vectors include prokaryotic or eukaryotic cells as described herein. For example, antibodies may be produced in bacteria, particularly if glycosylation and Fc effector functions are not required. For expression of antibody fragments and polypeptides in bacteria, see, for example, U.S. Patent Nos. 5,648,237, 5,789,199, and 5,840,523. (See also Charlton, Methods in Molecular Biology, Vol. 248 (BKC Lo, ed., Humana Press, Totowa, NJ, 2003), pp. 245-254, which describes the expression of antibody fragments in E. coli.) After expression, the antibody may be isolated in a soluble fraction from the bacterial cell paste, and may be further purified.

[0130] In addition to prokaryotes, eukaryotic microbes such as filamentous fungi or yeast, including fungal and yeast strains whose glycosylation pathways have been "humanized," resulting in the production of antibodies with partial or fully human glycosylation patterns, are suitable cloning or expression hosts for antibody-encoding vectors. See Gerngross, Nat. Biotech. 22:1409-1414 (2004) and Li et al., Nat. Biotech. 24:210-215 (2006).

[0131] Host cells derived from multicellular organisms (invertebrate and vertebrate) are also suitable for the expression of glycosylated antibodies. Examples of invertebrate cells include plants and insect cells. Numerous baculovirus strains have been identified for conjugation with insect cells, particularly for transformation of Spodoptera frugiperda cells.

[0132] Plant cell cultures can also be used as hosts. See, e.g., U.S. Patent Nos. 5,959,177, 6,040,498, 6,420,548, 7,125,978, and 6,417,429 (describing the PLANTIBODIES™ technology for producing antibodies in transgenic plants).

[0133] Vertebrate cells can also be used as hosts. For example, mammalian cell lines that have been adapted to grow in suspension may be useful. Other examples of useful mammalian host cell lines include SV40 transformed monkey kidney CV1 line (COS-7); human embryonic kidney lines (293 or 293 cells, e.g., as described in Graham et al., J. Gen Virol. 36:59 (1977)); baby hamster kidney cells (BHK); mouse Sertoli cells (TM4 cells, e.g., as described in Mather, Biol. Reprod. 23:243-251 (1980)); monkey kidney cells (CV1); African green monkey kidney cells (VERO-76); human cervical carcinoma cells (HELA); canine kidney cells (MDCK); Buffalo rat hepatocytes (BRL 3A); human lung cells (W138); human hepatocytes (Hep G2); mouse mammary carcinoma (MMT 060562); TRI cells (e.g., as described in Mather et al., Annals NY Acad. Sci. 383:44-68 (1982)). ; MRC5 cells; and FS4 cells. Other useful mammalian host cell lines include Chinese hamster ovary (CHO) cells, including DHFR- CHO cells (Urlaub et al., Proc. Natl. Acad. Sci. USA 77:4216 (1980)); and myeloma cell lines such as Y0, NS0, and Sp2 / 0. For a review of certain mammalian host cell lines suitable for antibody production, see, e.g., Yazaki and Wu, Methods in Molecular Biology, Vol. 248 (BKC Lo, ed., Humana Press, Totowa, NJ), pp. 255-268 (2003).

[0134] C. Assays Multispecific antigen binding molecules that bind FIX and / or FIXa and FX provided herein may be identified, screened, or characterized for physical / chemical properties and / or biological activity by various assays known in the art.

[0135] 1. Binding and other assays In one aspect, the antibodies of the invention are tested for their antigen binding activity by known methods, such as ELISA, Western blot, and the like.

[0136] In another aspect, a competitive assay can be used to identify antibodies that compete with any of the multispecific antibodies that bind to FIX and / or FIXa and FX described herein for binding to FIX and / or FIXa and FX. In certain embodiments, such competing antibodies bind to the same epitope (e.g., linear or conformational epitope) as that bound by the multispecific antibodies that bind to FIX and / or FIXa and FX described herein. Detailed exemplary methods for mapping the epitope to which an antibody binds are provided in Morris (1996) "Epitope Mapping Protocols," in Methods in Molecular Biology vol. 66 (Humana Press, Totowa, NJ).

[0137] In an exemplary competitive assay, immobilized FIX, FIXa or FX is incubated in a solution containing a first labeled antibody (e.g., any antibody described herein) that binds to FIX, FIXa or FX and a second unlabeled antibody that is tested for its ability to compete with the first antibody for binding to FIX, FIXa or FX. The second antibody may be present in a hybridoma supernatant. As a control, immobilized FIX, FIXa or FX is incubated in a solution containing the first labeled antibody but not the second unlabeled antibody. After incubation under conditions that allow binding of the first antibody to FIX, FIXa or FX, excess unbound antibody is removed and the amount of label bound to immobilized FIX, FIXa or FX is measured. If the amount of label bound to immobilized FIX, FIXa or FX is substantially reduced in the test sample compared to the control sample, it indicates that the second antibody competes with the first antibody for binding to FIX, FIXa or FX. See Harlow and Lane (1988) Antibodies: A Laboratory Manual ch.14 (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY).

[0138] 2.Activity measurement method In one aspect, a method for identifying a multispecific antigen-binding molecule that binds to FIX and / or FIXa and FX having biological activity is provided. Biological activity can include, for example, the activity of promoting FXa production. Also provided is a multispecific antigen-binding molecule that has such biological activity in vivo and / or in vitro.

[0139] In certain embodiments, the antibodies of the invention are tested for such biological activities. "FVIII cofactor functional replacement activity" means, for example, the activity of increasing absorbance in an FX activation reaction test using FIXa using a colorimetric method, and the activity of increasing the amount of thrombin generated calculated from a thrombin generation test using hemophilia A plasma.

[0140] Specifically, the FVIII cofactor functional substitution activity using colorimetric assay can be confirmed by evaluation in a measurement system consisting of the polyspecific antigen-binding molecule of the present invention, for example, FIXa, FX, synthetic substrate S-2222 (synthetic substrate for FXa), and phospholipids. For example, it can be measured by the following method. All reactions are carried out at room temperature. 5 μL of an antibody solution diluted with Tris-buffered saline (hereinafter referred to as TBSB) containing 0.1% bovine serum albumin is mixed with 5 μL of 150 ng / mL Human Factor IXa beta (Enzyme Research Laboratories), and then incubated at room temperature for 30 minutes in a 384-well plate. The enzyme reaction in this mixture is started by adding 5 μL of 24.7 μg / mL Human Factor X (Enzyme Research Laboratories), and stopped after 4 minutes by adding 5 μL of 0.5 M EDTA. The color reaction is started by adding 5 μL of a color-developing substrate solution. After a 30-minute color reaction, the change in absorbance at 405 nm was measured using a SpectroMax 340PC384 (Molecular Devices). The solvent for Human Factor IXa beta and Human Factor X was 4.0 μM phospholipid solution (SYSMEX CO.), 1.5 mM CaCl 2The TBSB contains 1.47 mg / mL of S-2222 (SEKISUI MEDICAL), a chromogenic substrate solution, which is dissolved in purified water at 1.47 mg / mL and used in this assay. This measurement system shows a correlation with the severity of disease and clinical symptoms in hemophilia A cases (Rosen S, Andersson M, Blomba¨ck M et al. Clinical applications of a chromogenic substrate method for determination of FVIII activity. Thromb Haemost 1985; 54: 811-23).

[0141] Specifically, the FVIII cofactor functional substitution activity using a thrombin generation test can be evaluated using a measurement system consisting of the polyspecific binding molecule of the present invention and, for example, FVIII-deficient plasma, activated blood coagulation factor XI, phospholipid, Fluo-buffer, and Fluo-Substrate (FluCa-Kit; a synthetic substrate for thrombin). For example, it can be measured by the following method. 8 μL of a bispecific antibody diluted with TBSB is added to 72 μL of FVIII-deficient plasma (George King), and incubated at room temperature for 30 minutes or more. Then, 20 μL of a trigger reagent containing 20 μM phospholipid and 5 ng / mL Human Factor XIa (Enzyme Research Laboratories) is added. Furthermore, 20 μL of a mixed solution of Fluo-Buffer and Fluo-Substrate from FluCa-Kit (Thrombinoscope) is added to start the coagulation reaction. The amount of thrombin generated can be evaluated using a thrombin generation fluorescence measurement and analysis system (Thrombinoscope). The thrombin generation test using hemophilia A plasma shows comprehensive coagulation ability in hemophilia A cases and correlates with clinical symptoms of the disease. (Shima M, Matsumoto T & Ogiwara K. New assays for monitoring haemophilia treatment. Haemophilia 2008; 14: 83-92).

[0142] "FIX activation inhibitory activity" refers to the absorbance reduction in the FIX activation reaction by FXIa using a colorimetric assay. Specifically, it can be performed as follows. 5 μL of the antibody solution diluted with TBSB is mixed with 5 μL of 3 U / mL Human Factor IX (CrisMachine M, Japan Blood Products Organization), and then incubated at room temperature for 30 minutes in a 384-well plate. The enzyme reaction in this mixture is started by adding 5 μL of 90 ng / mL Human Factor XIa (Enzyme Research Laboratories), and after 60 minutes, it is stopped by adding 5 μL of 0.5 M EDTA. The color reaction is started by adding 10 μL of a color substrate solution. After the color reaction for 60 minutes, the change in absorbance at 405 nm is measured using a SpectroMax 340PC384 (Molecular Devices). The solvent for Human Factor IX and Human Factor XIa was 6.0 μM phospholipid solution (SYSMEX CO.), 1.5 mM CaCl 2 The chromogenic substrate solution, Spectrozyme FIXa (Sekisui Diagnostics), was dissolved in purified water to give a 6.7 mM solution, which was then mixed with ethylene glycol in a ratio of 5:8 for use in this assay.

[0143] The binding evaluation of the antibody to ECM (extracellular matrix) can be performed by the following procedure with reference to US20140080153 (WO2012093704). ECM Phenol red free (BD Matrigel #6137013) is diluted to 2 mg / mL with TBS, and 5 μL is dropped into the center of each well of an ECL measurement plate (L15XB-6, MSD high bind) cooled on ice. Then, cover with a plate seal and leave at 4 ° C overnight. The plate with the immobilized ECM is returned to room temperature, and 150 μL of ECL Blocking Buffer (20 mM ACES, 150 mM NaCl, pH 7.4 or pH 5.8 with 0.5% BSA and 0.05% Tween 20) is added to each well, and the plate is left at room temperature for at least 2 hours or at 4 ° C overnight. Next, dilute the antibody sample to 9μg / mL using ACES-T (20mM ACES, 150mM NaCl, pH7.4 or pH5.8 with 0.05% Tween 20). Dilute the secondary antibody to 2μg / mL with ECLDB (ACES with 0.1% BSA and 0.01% Tween 20) and add 25μL of the antibody solution to the round-bottom plate with 50μL of ECLDB dispensed in each well. Remove the ECL Blocking Buffer from the ECM plate containing the ECL Blocking Buffer by inverting, and add 50μL of the antibody solution to this plate. Then, stir at room temperature for 1 hour. After removing the sample by inverting, add 50μL of 0.25% Gluteraldehyde (prepared with ACES-T) to each well and leave at room temperature for 10 minutes. After washing each well three times with PBS-T (PBS with 0.05% Tween 20), add 50 μL of secondary antibody diluted to 1 μg / mL with ECLDB to each well and stir for 1 hour at room temperature in the dark. Then, add 150 μL of READ buffer (MSD) to each well and detect the sulfo-tag luminescence signal with MESO SECTOR S600 (Meso Scale Discovery).

[0144] F. Pharmaceutical Formulations Pharmaceutical formulations of the multispecific antigen-binding molecules that bind FIX and / or FIXa and FX described herein are prepared in the form of a lyophilized formulation or aqueous solution by mixing the multispecific antigen-binding molecules having the desired purity with any one or more pharma- ceutically acceptable carriers (Remington's Pharmaceutical Sciences 16th edition, Osol, A. Ed. (1980)). Pharmaceutically acceptable carriers are generally non-toxic to recipients at the dosages and concentrations employed, and include, but are not limited to, the following: buffers such as phosphate, citrate, and other organic acids; antioxidants, including ascorbic acid and methionine; preservatives (octadecyldimethylbenzyl ammonium chloride; hexamethonium chloride; benzalkonium chloride; benzethonium chloride; phenol, butyl, or benzyl alcohol; alkyl parabens such as methyl or propyl paraben; catechol; resorcinol; cyclohexanol; 3-pentanol; and m-cresol, etc.; small (less than about 10 residues) polypeptides; proteins such as serum albumin, gelatin, or immunoglobulins; hydrophilic polymers such as polyvinylpyrrolidone; amino acids such as glycine, glutamine, asparagine, histidine, arginine, or lysine; monosaccharides, disaccharides, and other carbohydrates, including glucose, mannose, or dextrin; chelating agents such as EDTA; sugars such as sucrose, mannitol, trehalose, sorbitol; salt-forming counterions such as sodium; metal complexes (e.g., Zn-protein complexes); and / or non-ionic surfactants such as polyethylene glycol (PEG). Exemplary pharmacologic carriers herein further include interstitial drug dispersing agents, such as soluble neutral activated hyaluronidase glycoproteins (sHASEGPs) (e.g., human soluble PH-20 hyaluronidase glycoproteins, such as rHuPH20 (HYLENEX®, Baxter International, Inc.)).Certain exemplary sHASEGPs and methods of their use (including rHuPH20) are described in U.S. Patent Application Publication Nos. 2005 / 0260186 and 2006 / 0104968. In one aspect, a sHASEGP is combined with one or more additional glycosaminoglycanases, such as chondroitinases.

[0145] Exemplary lyophilized antibody formulations are described in U.S. Patent No. 6,267,958. Aqueous antibody formulations include those described in U.S. Patent No. 6,171,586 and WO2006 / 044908, the latter formulations including a histidine-acetate buffer.

[0146] The formulations herein may contain more than one active ingredient if necessary for the particular indication being treated. Preferably, they are accompanied by complementary activities that do not adversely affect each other. For example, it is desirable to further provide FVIII, FVII, FIX, TFPI inhibitors, siRNA targeting antithrombin, more specifically Advate, Adynovate, Feiba, NovoSeven, NovoEight, N8-GP, N9-GP, Concizumab, Elocta, Fitusiran. Such active ingredients are present in an amount that is effective for the intended purpose and in a suitable combination. In addition, FVIII, FVII and FIX may include their Fc fusions and PEG fusions.

[0147] The active ingredient may be incorporated into microcapsules (e.g., hydroxymethylcellulose or gelatin microcapsules, and poly(methyl methacrylate) microcapsules, respectively) prepared, for example, by droplet formation (coacervation) techniques or by interfacial polymerization, into colloidal drug delivery systems (e.g., liposomes, albumin microspheres, microemulsions, nanoparticles, and nanocapsules), or into macroemulsions. Such techniques are disclosed in Remington's Pharmaceutical Sciences 16th edition, Osol, A. Ed. (1980).

[0148] Sustained-release preparations may be prepared. Suitable examples of sustained-release preparations include semipermeable matrices of solid hydrophobic polymers containing the multispecific antigen-binding molecules, which matrices are in the form of shaped articles, e.g., films, or microcapsules.

[0149] Preparations to be used for in vivo administration are typically sterile. Sterility is readily accomplished, for example, by filtration through sterile filtration membranes.

[0150] G. Therapeutic Methods and Compositions Any of the multispecific antigen binding molecules that bind FIX and / or FIXa and FX provided herein may be used in therapeutic methods. In one aspect, a multispecific antigen binding molecule that binds FIX and / or FIXa and FX is provided for use as a medicament. In a further aspect, a multispecific antigen binding molecule that binds FIX and / or FIXa and FX is provided for use in the treatment of bleeding, a bleeding disorder, or a bleeding-induced disorder. In a particular embodiment, a multispecific antigen binding molecule that binds FIX and / or FIXa and FX is provided for use in a treatment method. In a particular embodiment, the present invention provides a multispecific antigen binding molecule that binds FIX and / or FIXa and FX for use in a method of treating an individual with bleeding, a bleeding disorder, or a bleeding-induced disorder, comprising administering to the individual an effective amount of a multispecific antigen binding molecule that binds FIX and / or FIXa and FX. In one such embodiment, the method further comprises administering to the individual an effective amount of at least one additional therapeutic agent (e.g., as described below). In a further embodiment, the present invention provides a multispecific antigen binding molecule that binds FIX and / or FIXa and FX for use in replacing FVIII cofactor function. In a particular embodiment, the present invention provides a multispecific antigen-binding molecule that binds FIX and / or FIXa and FX for use in a method of replacing FVIII cofactor function in an individual, the method comprising administering to the individual an effective amount of the multispecific antigen-binding molecule that binds FIX and / or FIXa and FX to replace FVIII cofactor function. An "individual" according to any of the above embodiments is preferably a human. In the present invention, bleeding, a disease accompanied by bleeding, or a disease caused by bleeding is preferably a disease that develops and / or progresses due to a decrease or deficiency in the activity of FVIII and / or activated blood coagulation factor VIII (FVIIIa). Examples of such diseases include, but are not limited to, the above-mentioned hemophilia A, hemophilia B, hemophilia C, diseases in which inhibitors against FVIII / FVIIIa have appeared, acquired hemophilia, von Willebrand's disease, etc.

[0151] In a further aspect, the present invention provides the use of a multispecific antigen binding molecule that binds FIX and / or FIXa and FX in the manufacture or preparation of a medicament. In one embodiment, the medicament is for the treatment of bleeding, a bleeding disorder, or a bleeding-related disorder. In a further embodiment, the medicament is for use in a method of treating bleeding, a bleeding disorder, or a bleeding-related disorder, comprising administering an effective amount of the medicament to an individual having bleeding, a bleeding disorder, or a bleeding-related disorder. In one such embodiment, the method further comprises administering to the individual an effective amount of at least one additional therapeutic agent (e.g., as described below). In a further embodiment, the medicament is for replacing FVIII cofactor function. In a further embodiment, the medicament is for use in a method of replacing FVIII cofactor function in an individual, comprising administering to the individual an effective amount of the medicament to replace FVIII cofactor function. An "individual" according to any of the above embodiments may be a human. In the present invention, bleeding, a disease accompanied by bleeding, or a disease caused by bleeding is preferably a disease that develops and / or progresses due to a decrease or deficiency in the activity of FVIII and / or FVIIIa, such as the above-mentioned hemophilia A, hemophilia B, hemophilia C, diseases in which inhibitors against FVIII / FVIIIa have appeared, acquired hemophilia, von Willebrand's disease, etc., but is not particularly limited to these diseases.

[0152] In a further aspect, the present invention provides a method for treating bleeding, a bleeding disorder, or a bleeding-related disorder. In one embodiment, the method comprises administering to an individual having such bleeding, a bleeding disorder, or a bleeding-related disorder an effective amount of a multispecific antigen binding molecule that binds FIX and / or FIXa and FX. In one such embodiment, the method further comprises administering to the individual an effective amount of at least one additional therapeutic agent (as described below). An "individual" according to any of the above embodiments may be a human.

[0153] In a further aspect, the present invention provides a method for replacing FVIII cofactor function in an individual. In one embodiment, the method comprises administering to the individual an effective amount of a multispecific antigen-binding molecule that binds FIX and / or FIXa and FX to replace FVIII cofactor function. In one embodiment, the "individual" is a human.

[0154] In a further aspect, the present invention provides pharmaceutical formulations (e.g., for use in any of the therapeutic methods described above) comprising any of the multispecific antigen binding molecules that bind FIX and / or FIXa and FX provided herein. In one embodiment, the pharmaceutical formulation comprises any of the multispecific antigen binding molecules that bind FIX and / or FIXa and FX provided herein and a pharma- ceutically acceptable carrier. In another embodiment, the pharmaceutical formulation comprises any of the multispecific antigen binding molecules that bind FIX and / or FIXa and FX provided herein and at least one additional therapeutic agent (e.g., as described below).

[0155] The multispecific antigen-binding molecules of the present invention can be used alone or in combination with other agents in therapy. For example, the multispecific antigen-binding molecules of the present invention can be co-administered with at least one additional therapeutic agent. In certain embodiments, the additional therapeutic agent is, for example, FVIII, FVII, FIX, TFPI inhibitors, siRNA targeting antithrombin, more specifically Advate, Adynovate, Feiba, NovoSeven, NovoEight, N8-GP, N9-GP, Concizumab, Elocta, Fitusiran. In addition, FVIII, FVII and FIX can be Fc fusions or PEG fusions thereof.

[0156] Combination therapy as described above includes combined administration (two or more therapeutic agents in the same or separate formulations) and separate administration, where administration of the multispecific antigen-binding molecule of the present invention may precede, be concurrent with, and / or be subsequent to administration of the additional therapeutic agent. In one embodiment, administration of the multispecific antigen-binding molecule that binds FIX and / or FIXa and FX and administration of the additional therapeutic agent are performed within about 1 month, 2 months, 3 months, 4 months, 5 months, or 6 months, or within about 1, 2, or 3 weeks, or within about 1, 2, 3, 4, 5, or 6 days of each other.

[0157] The multispecific antigen-binding molecules of the present invention (and any additional therapeutic agents) may be administered by any suitable means, including parenteral, pulmonary, and nasal administration, and, if desired for localized treatment, intralesional administration. Parenteral injections include intramuscular, intravenous, intraarterial, intraperitoneal, or subcutaneous administration. Dosing may be by any suitable route, for example, by injection, such as intravenous or subcutaneous injection, depending in part on whether administration is brief or chronic. Various dosing schedules are contemplated herein, including, but not limited to, single or repeated doses over various time periods, bolus administration, and pulse infusion.

[0158] The multispecific antigen-binding molecules of the present invention are formulated, dosed, and administered in a manner consistent with good medical practice. Factors to be considered in this regard include the particular disorder being treated, the particular mammal being treated, the clinical condition of the individual patient, the cause of the disorder, the site of delivery of the agent, the method of administration, the schedule of administration, and other factors known to medical practitioners. The multispecific antigen-binding molecules are optionally, but not necessarily, formulated with one or more agents currently used to prevent or treat the disorder in question. The effective amount of such other agents depends on the amount of multispecific antigen-binding molecules present in the formulation, the type of disorder or treatment, and other factors discussed above. These are typically used in the same dosages and routes of administration as described herein, or about 1 to 99% of the dosages described herein, or in any dosage and by any route as empirically / clinically deemed appropriate.

[0159] For the prevention or treatment of disease, the appropriate dose of the multispecific antigen-binding molecule of the present invention (when used alone or with one or more other additional therapeutic agents) will depend on the type of disease being treated, the type of multispecific antigen-binding molecule, the severity and course of the disease, whether the multispecific antigen-binding molecule is administered for prophylactic or therapeutic purposes, medical history, the patient's clinical history and response to the multispecific antigen-binding molecule, and the discretion of the attending physician. The multispecific antigen-binding molecule is suitably administered to the patient at one time or over a series of treatments. Depending on the type and severity of the disease, for example, about 1 μg / kg to 15 mg / kg (e.g., 0.1 mg / kg to 10 mg / kg) of the multispecific antigen-binding molecule may be the initial candidate dose for administration to the patient, whether by one or more separate administrations or by continuous infusion. One typical daily dose may range from about 1 μg / kg to 100 mg / kg or more, depending on the factors mentioned above. In the case of repeated administration over several days or longer, depending on the circumstances, treatment is usually maintained until a desired suppression of disease symptoms occurs. One exemplary dose of the multispecific antigen-binding molecule is in the range of about 0.05 mg / kg to about 10 mg / kg. Thus, one or more doses of about 0.5 mg / kg, 2.0 mg / kg, 4.0 mg / kg, or 10 mg / kg (or any combination thereof) may be administered to the patient. Such doses may be administered intermittently, for example, every week or every three weeks (e.g., such that the patient receives about 2 to about 20, or, for example, about 6 doses of the multispecific antigen-binding molecule). A high initial loading dose may be administered followed by one or more lower doses. However, other dosing regimens may also be useful. The progress of this therapy is easily monitored by conventional techniques and measurements.

[0160] It will be appreciated that any of the above-mentioned formulations or therapeutic methods may be practiced using an immunoconjugate of the invention instead of or in addition to a multispecific antigen binding molecule that binds FIX and / or FIXa and FX.

[0161] H.Product In another aspect of the present invention, an article of manufacture is provided that includes materials useful for the treatment, prevention, and / or diagnosis of the above-mentioned disorders. The article of manufacture includes a container and a label on the container or a package insert associated with the container. Preferred containers include, for example, bottles, vials, syringes, IV solution bags, and the like. The containers may be formed from a variety of materials, such as glass or plastic. The container may hold the composition alone or in combination with another composition effective for the treatment, prevention, and / or diagnosis of a condition, and may have a sterile access port (e.g., the container may be an intravenous solution bag or vial having a stopper pierceable by a hypodermic needle). At least one active ingredient in the composition is a multispecific antigen-binding molecule of the present invention. The label or package insert indicates that the composition is used to treat the condition of choice. The article of manufacture may further comprise: (a) a first container with a composition comprising the multispecific antigen-binding molecule of the invention contained therein; and (b) a second container with a composition comprising an additional cytotoxic or otherwise therapeutic agent contained therein. The article of manufacture in this aspect of the invention may further comprise a package insert indicating that the composition may be used to treat a particular condition. Alternatively or additionally, the article of manufacture may further comprise a second (or third) container comprising a pharma- ceutically acceptable buffer, such as bacteriostatic water for injection (BWFI), phosphate buffered saline, Ringer's solution, and dextrose solution. It may further comprise other equipment desirable from a commercial or user standpoint, such as other buffers, diluents, filters, needles, and syringes.

[0162] Another aspect of the present invention relates to an antigen-binding molecule in which the association between the heavy chain and the light chain is controlled, a method for producing an antigen-binding molecule in which the association between the heavy chain and the light chain is controlled, and a method for controlling the association between the heavy chain and the light chain of an antigen-binding molecule.

[0163] The antigen-binding molecule of the present invention is an antigen-binding molecule in which the association of heavy and light chains is controlled, the heavy and light chains constituting the antigen-binding molecule are a combination of a desired heavy and light chain, and amino acid residues at specific positions in the heavy-chain constant region (CH1) and light-chain constant region (CL) are amino acid residues that repel each other in terms of charge (have the same type of charge). In the present invention, by changing the amino acid residues at specific positions of CH1 and CL of an undesired heavy chain and light chain combination to amino acid residues that repel each other in terms of charge (having the same charge), it is possible to utilize the repulsion of the charge to prevent the formation of an undesired heavy chain and light chain combination, and as a result, it is possible to form a desired heavy chain and light chain combination.

[0164] In the present invention, the terms "controlling the association" and "the association is controlled" refer to controlling the association to a desired state, and more specifically, refer to controlling the association to prevent the formation of an undesirable association between a heavy chain and a light chain.

[0165] In the present invention, the term "interface" generally refers to the surface of association (interaction), and the amino acid residues forming the interface generally refer to one or more amino acid residues contained in the polypeptide region involved in the association, and more preferably to amino acid residues that approach each other during association and participate in the interaction. Specific examples of such interactions include cases in which amino acid residues that approach each other during association form hydrogen bonds, electrostatic interactions, salt bridges, etc.

[0166] In the present invention, the "amino acid residues forming an interface" refers, in more detail, to amino acid residues contained in a polypeptide region that constitutes the interface. An example of a polypeptide region that constitutes the interface is a polypeptide region that is responsible for selective binding between molecules in an antigen-binding molecule (e.g., an antibody), a ligand, a receptor, a substrate, etc. Specific examples of antigen-binding molecules include a heavy chain constant region, a heavy chain variable region, a light chain constant region, a light chain variable region, etc.

[0167] In the present invention, the "modification" of an amino acid residue specifically refers to substituting an original amino acid residue with another amino acid residue, deleting an original amino acid residue, adding a new amino acid residue, etc., and preferably refers to substituting an original amino acid residue with another amino acid residue.

[0168] In a preferred embodiment of the antigen-binding molecule of the present invention, amino acid residues at predetermined positions in CH1 and CL of an undesired heavy chain and light chain combination before association control are antigen-binding molecules having amino acid residues that repel each other electrically (having the same type of charge). It is considered that by modifying the amino acid residues in the antigen-binding molecule to amino acid residues that repel each other electrically (having the same type of charge), the association between these amino acid residues is inhibited by the repulsive force of the charges.

[0169] Therefore, in the above-mentioned antigen-binding molecule, the amino acid residues to be modified are preferably those that are close to each other during association between the polypeptide regions that form the interface.

[0170] The amino acid residues that are close to each other during association can be found, for example, by analyzing the three-dimensional structure of the polypeptide and examining the amino acid sequence of the polypeptide region that forms an interface during association of the polypeptide. Amino acid residues that are close to each other at the interface are preferred targets for "modification" in the antigen-binding molecules of the present invention.

[0171] Among amino acids, there are known charged amino acids. Lysine (K), arginine (R), and histidine (H) are generally known as positively charged amino acids (positively charged amino acids). Aspartic acid (D), glutamic acid (E), and the like are known as negatively charged amino acids (negatively charged amino acids). In addition, alanine (A), asparagine (N), cysteine ​​(C), glutamine (Q), glycine (G), isoleucine (I), leucine (L), methionine (M), phenylalanine (F), proline (P), serine (S), threonine (T), tryptophan (W), tyrosine (Y), valine (V), and the like are known as uncharged or nonpolar amino acids. Therefore, in the present invention, the amino acids which are mutually repulsive in terms of charge (have the same kind of charge) are: (1) One amino acid is a positively charged amino acid and the other is also a positively charged amino acid (2) One amino acid is a negatively charged amino acid and the other amino acid is also a negatively charged amino acid.

[0172] Amino acid modifications can be carried out by various methods known in the art. These methods include, but are not limited to, site-directed mutagenesis (Hashimoto-Gotoh, T, Mizuno, T, Ogasahara, Y, and Nakagawa, M. (1995) An oligodeoxyribonucleotide-directed dual amber method for site-directed mutagenesis. Gene 152, 271-275; Zoller, MJ, and Smith, M.(1983) Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors.Methods Enzymol. 100, 468-500; Kramer, W, Drutsa, V, Jansen, HW, Kramer, B, Pflugfelder, M, and Fritz, HJ(1984) The gapped duplex DNA approach to oligonucleotide-directed mutation construction. Nucleic Acids Res. 12, 9441-9456; Kramer W, and Fritz Mutations can be performed by methods such as PCR mutagenesis and cassette mutagenesis, etc. (HJ (1987) Oligonucleotide-directed construction of mutations via gapped duplex DNA Methods. Enzymol. 154, 350-367; Kunkel, TA (1985) Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc Natl Acad Sci US A. 82, 488-492), PCR mutagenesis, and cassette mutagenesis.

[0173] Examples of amino acid modification include modification of uncharged or nonpolar amino acids to positively charged amino acids, modification of uncharged or nonpolar amino acids to negatively charged amino acids, modification of positively charged amino acids to negatively charged amino acids, and modification of negatively charged amino acids to positively charged amino acids. Note that modification of uncharged or nonpolar amino acids to other uncharged or nonpolar amino acids, modification of positively charged amino acids to other positively charged amino acids, and modification of negatively charged amino acids to other negatively charged amino acids are also included in the amino acid modification of the present invention.

[0174] In the present invention, one or more amino acid modifications may be added to each of the heavy and light chains, and the number of modifications added to the heavy and light chains may be the same or different.

[0175] In the present invention, the amino acid modification may be such that multiple modifications to positively charged amino acids are added to one of the heavy chain or the light chain, and multiple modifications to negatively charged amino acids are added to the other. Also, multiple modifications to positively charged amino acids and multiple modifications to negatively charged amino acids may be added to the same chain of the heavy chain or the light chain. In this modification, modifications to uncharged amino acids or nonpolar amino acids, and modifications to uncharged amino acids or nonpolar amino acids may be appropriately combined.

[0176] In the modification of the present invention, for example, the amino acids of one chain can be used as is without modification, in which case it is not necessarily necessary to modify both the heavy chain and the light chain, and modification may be made to only one chain.

[0177] The number of amino acid residues to be modified in the antigen-binding molecule of the present invention is not particularly limited, but for example, when modifying an antibody constant region, it is preferable to modify as few amino acid residues as possible so as not to reduce antigen-binding activity or increase immunogenicity. The above "few" refers to, for example, about 1 to 30, preferably about 1 to 20, more preferably 1 to 15, and most preferably 1 to 5.

[0178] The light chain constant region of the antigen-binding molecule of the present invention is preferably a human light chain constant region. The light chain constant region of the antigen-binding molecule includes, for example, IgK (Kappa), IgL1, IgL2, IgL3, IgL6, and IgL7 (Lambda) types of constant regions. The light chain constant region of the antigen-binding molecule of the present invention is not particularly limited. When multiple types of light chains are used, each light chain may be a different type of light chain, for example, Kappa and Lambda. As for the human IgK (Kappa) constant region and the human IgL7 (Lambda) constant region, multiple allotype sequences due to genetic polymorphism are described in Sequences of proteins of immunological interest, NIH Publication No. 91-3242, and any of these may be used in the present invention.

[0179] In order to improve the function or stability of an antigen-binding molecule, the antibody constant region, particularly the heavy chain constant region, may be modified as necessary. Modifications for improving the function of an antigen-binding molecule include, for example, modifications that enhance or weaken the binding between the antigen-binding molecule and Fcγ receptor (FcγR), modifications that enhance or weaken the binding between the antigen-binding molecule and FcRn, and modifications that enhance or weaken the cytotoxic activity (e.g., ADCC activity, CDC activity, etc.) of the antigen-binding molecule. Modifications may also include modifications that improve the heterogeneity of the antigen-binding molecule, and modifications that improve immunogenicity and / or pharmacokinetics.

[0180] Additionally, heterogeneity in the C-terminal sequence of the heavy chain of an IgG antibody has been reported, including the deletion of a C-terminal lysine residue and the amidation of the C-terminal carboxyl group due to the deletion of both glycine and lysine, the two C-terminal amino acids (Anal Biochem. 2007 Jan 1;360(1):75-83.). Therefore, in the present invention, it is preferable to use an IgG in which lysine or lysine and glycine at the C-terminus has been deleted in order to reduce heterogeneity at the C-terminus of the heavy chain.

[0181] Chimeric and humanized antibodies that utilize human-derived sequences have reduced antigenicity in the human body and are therefore considered useful when administered to humans for therapeutic purposes.

[0182] Preferred antigen-binding molecules of the present invention include, for example, heteromultimers having two or more types of CH1 and two or more types of CL. The heteromultimer preferably binds to two or more types of epitopes, and examples thereof include multispecific antibodies.

[0183] A preferred example of the multispecific antibody of the present invention is a bispecific antibody. That is, a preferred embodiment of the antigen-binding molecule of the present invention is, for example, a bispecific antibody composed of two types of heavy chains (a first heavy chain and a second heavy chain) and two types of light chains (a first light chain and a second light chain).

[0184] More specifically, the "bispecific antibody" of the preferred embodiment of the antigen-binding molecule of the present invention is as follows: the "first heavy chain" refers to one of the two heavy chains (H chains) forming an antibody, and the second H chain refers to the other H chain different from the first H chain. In other words, one of the two H chains can be arbitrarily selected as the first H chain and the other as the second H chain. Similarly, the "first light chain" refers to one of the two light chains (L chains) forming a bispecific antibody, and the second L chain refers to the other L chain different from the first L chain, and one of the two L chains can be arbitrarily selected as the first L chain and the other as the second L chain. Usually, the first L chain and the first H chain are derived from the same antibody that binds to a certain antigen (or epitope), and the second L chain and the second H chain are also derived from the same antibody that binds to a certain antigen (or epitope). Here, the L chain-H chain pair formed by the first H chain and L chain is called the first pair, and the L chain-H chain pair formed by the second H chain and L chain is called the second pair. The antigen (or epitope) used in preparing the antibody from which the second pair is derived is preferably different from that used in preparing the antibody from which the first pair is derived. That is, the antigens recognized by the first pair and the second pair may be the same, but it is preferable that they bind to different antigens (or epitopes). In this case, it is preferable that the H chain and L chain of the first pair and the second pair have different amino acid sequences from each other. When the first pair and the second pair bind to different epitopes, the first pair and the second pair may recognize completely different antigens, or may recognize different sites (different epitopes) on the same antigen. Alternatively, one may recognize an antigen such as a protein, peptide, gene, or sugar, and the other may recognize a cytotoxic substance such as a radioactive substance, a chemotherapeutic agent, or a cell-derived toxin. However, if one wishes to produce an antibody having a pair formed by a specific combination of H chain and L chain, the specific H chain and L chain can be arbitrarily determined as the first pair and the second pair.

[0185] Below, the case of an IgG bispecific antibody having two types of heavy chain constant regions CH1 (CH1-A and CH1-B) and two types of light chain constant regions (CL-A and CL-B) will be explained in more detail, but the present invention can be similarly applied to other antibodies.

[0186] When it is desired to obtain a bispecific antibody that recognizes one epitope via the first CH1-A and the first CL-A and binds to another epitope via the second CH1-B and the second CL-B, theoretically, ten types of antibody molecules can be produced by expressing each of the four chains in the production of the antibody.

[0187] In this case, for example, by controlling the association between CH1-A and CL-B and / or CH1-B and CL-A to be inhibited, it is possible to preferentially obtain the desired antibody molecule.

[0188] For example, an example can be given in which the amino acid residues forming the interface between CH1-A and CL-B are modified to amino acid residues having a positive charge, and the amino acid residues forming the interface between CH1-B and CL-A are modified to amino acid residues having a negative charge. With this modification, the unintended association between CH1-A and CL-B is inhibited because the amino acid residues forming the interface are both positively charged, and the association between CH1-B and CL-A is also inhibited because the amino acid residues forming the interface are both negatively charged, and the unintended association between CH1-A and CL-B and the association between CH1-B and CL-A are inhibited because the amino acid residues forming the interface have the same charge. As a result, an antibody in which the intended association between CH1-A and CL-A and the intended association between CH1-B and CL-B have occurred can be efficiently obtained. In addition, the intended association between CH1-A and CL-A is promoted because the amino acid residues forming the interface have different charges, and the intended association between CH1-B and CL-B is also promoted because the amino acid residues forming the interface have different charges. As a result, antibodies exhibiting the desired association can be obtained efficiently.

[0189] In addition, when the amino acid residues forming the interface between CL-A and CH1-B are mutually uncharged amino acids or nonpolar amino acids, an example can be given in which the amino acid residues forming the interface between CH1-A and CL-B are modified to positively charged amino acid residues. This modification inhibits the association of unintended CH1-A with CL-B because both of the amino acid residues forming the interface are positively charged. On the other hand, the intended association of CH1-A with CL-A and the intended association of CH1-B with CL-B are more likely to occur because the amino acid residues forming the interface are amino acids that do not repel each other in terms of charge, compared to when they are amino acids that repel each other in terms of charge. As a result, an antibody in which the intended association of CH1-A with CL-A and the intended association of CH1-B with CL-B has occurred can be efficiently obtained. In this example, when the amino acid residues forming the interface between CL-A and CH1-B are not mutually uncharged amino acids or nonpolar amino acids, they may be modified to be mutually uncharged amino acids or nonpolar amino acids.

[0190] In addition, when the amino acid residues forming the interface between CL-B and CH1-B are uncharged or nonpolar amino acids in CH1-B, one of the amino acid residues forming the interface between CH1-A and CL-A can be modified to an amino acid residue having a positive charge and the other having a negative charge, and a modification is added to CL-B to have the same charge as the modification in which the amino acid residues forming the interface between CL-B and CH1-B are added to CH1-A. With this modification, the intended association between CH1-A and CL-A is promoted due to the combination of positive and negative charges in the amino acid residues forming the interface, while the intended association between CH1-B and CL-B is not inhibited because the amino acid residues forming the interface are amino acids that do not repel each other in terms of charge. As a result, an antibody in which the intended association between CH1-A and CL-A and the intended association between CH1-B and CL-B have occurred can be efficiently obtained. In this example, if the amino acid residues forming the interface between CL-B and CH1-B are not uncharged or nonpolar amino acids in CH1-B, they may be modified to become uncharged or nonpolar amino acids.

[0191] Furthermore, by utilizing the association regulation of the present invention, it is also possible to suppress association between CH1s (CH1-A and CH1-B) or between CLs (CL-A and CL-B). Those skilled in the art can appropriately know the types of amino acid residues that are close to each other at the interface between CH1 and CL when associated with a desired polypeptide whose association is to be controlled by the present invention.

[0192] Furthermore, a person skilled in the art can appropriately obtain sequences that can be used as antibody CH1 or CL in organisms such as humans, monkeys, mice, and rabbits by using public databases, etc. More specifically, amino acid sequence information of CH1 or CL can be obtained by the means described in the Examples below.

[0193] For example, in the bispecific antibody shown in the Examples below, the following combinations can be mentioned as specific examples of amino acid residues that are close (facing or in contact) at the interface between CH1 and CL when associated. Glutamine (Q) at position 175 (EU numbering) of CH1 and threonine (T) or serine (S) at position 180 (Kabat numbering) of the corresponding CL Glutamine (Q) at position 175 (EU numbering) of CH1 and threonine (T) or serine (S) at position 131 (Kabat numbering) of CL that faces (contacts) it Glutamine (Q) at position 175 (EU numbering) of CH1 and serine (S) or threonine (T) at position 131 (Kabat numbering) and serine (S) or threonine (T) at position 180 (Kabat numbering) of the opposing (contacting) CL Lysine (K) at EU numbering position 147 and glutamine (Q) at EU numbering position 175 of CH1, and serine (S) or threonine (T) at Kabat numbering position 131 and serine (S) or threonine (T) at Kabat numbering position 180 of CL that face each other (contact)

[0194] In the present invention, the numbers described as EU numbering are written according to EU numbering (Sequences of proteins of immunological interest, NIH Publication No. 91-3242). In the present invention, the "amino acid residue at position X according to EU numbering" and "amino acid at position X according to EU numbering" (X is any number) can also be read as "amino acid residue corresponding to position X according to EU numbering" and "amino acid corresponding to position X according to EU numbering". As shown in the Examples below, by modifying these amino acid residues and carrying out the method of the present invention, desired antigen-binding molecules can be preferentially obtained.

[0195] In one aspect, the present invention provides an antigen-binding molecule in which the association of a heavy chain and a light chain is controlled, wherein one or more pairs of amino acid residues selected from the group consisting of the pairs of amino acid residues shown in the following (a) to (c) in the heavy chain and the light chain are amino acid residues that are mutually electrically repulsive: (a) an amino acid residue at position 175 in the EU numbering system contained in CH1, and an amino acid residue at position 180 in the Kabat numbering system contained in CL; (b) an amino acid residue at position 175 (EU numbering) contained in CH1, and an amino acid residue at position 131 (Kabat numbering) contained in CL; (c) amino acid residues at positions 147 and 175 in the EU numbering system contained in CH1, and amino acid residues at positions 131 and 180 in the Kabat numbering system contained in CL.

[0196] In the above-mentioned antigen-binding molecule, the "mutually charge-repulsive amino acid residues" or "amino acid residues having the same electric charge" are preferably selected from amino acid residues included in either group (X) or (Y) below, for example: (X) glutamic acid (E) or aspartic acid (D), (Y) lysine (K), arginine (R) or histidine (H).

[0197] In the above-mentioned antigen-binding molecule, specific examples of pairs of amino acid residues that are mutually electrically repulsive include the following pairs of amino acid residues: (a) an amino acid residue at position 175 (EU numbering) contained in CH1, and an amino acid residue at position 180 (EU numbering) contained in CL; (b) an amino acid residue at position 175 (EU numbering) contained in CH1, and an amino acid residue at position 131 (Kabat numbering) contained in CL; (c) amino acid residues at positions 147 and 175 in the EU numbering system contained in CH1, and amino acid residues at positions 131 and 180 in the Kabat numbering system contained in CL; (d) an amino acid residue at position 175 according to the EU numbering system which is contained in CH1, and an amino acid residue at positions 131 and 180 according to the Kabat numbering system which is contained in CL.

[0198] The antigen-binding molecule includes a heavy chain and a light chain, and one or more pairs of amino acid residues selected from the group consisting of the pairs of amino acid residues shown in the following (a1) to (c2) are amino acid residues that repel each other electrically: (a1) the amino acid residue at position 175 (EU numbering) in CH1 is glutamic acid (E) or aspartic acid (D), and the amino acid residue at position 180 (EU numbering) in CL is glutamic acid (E) or aspartic acid (D); (a2) an amino acid residue at position 175 (EU numbering) in CH1 is lysine (K), histidine (H), or arginine (R), and an amino acid residue at position 180 (EU numbering) in CL is lysine (K), histidine (H), or arginine (R); (b1) an amino acid residue in CH1 at position 175 (EU numbering) is glutamic acid (E) or aspartic acid (D), and an amino acid residue in CL at position 131 (EU numbering) is glutamic acid (E) or aspartic acid (D); (b2) an amino acid residue at position 175 (EU numbering) in CH1 is lysine (K), histidine (H), or arginine (R), and an amino acid residue at position 131 (EU numbering) in CL is lysine (K), histidine (H), or arginine (R); (c1) the amino acid residues at positions 147 and 175 (EU numbering) in CH1 are glutamic acid (E) or aspartic acid (D), respectively, and the amino acid residues at positions 131 and 180 (EU numbering) in CL are glutamic acid (E) or aspartic acid (D), respectively; (c2) The amino acid residues at positions 147 and 175 (EU numbering) in CH1 are lysine (K), histidine (H), or arginine (R), respectively, and the amino acid residues at positions 131 and 180 (EU numbering) in CL are lysine (K), histidine (H), or arginine (R), respectively.

[0199] In the above antigen-binding molecule, specific examples of amino acid residues that are electrically repulsive to each other include the following amino acid residues: (a1) the amino acid residue at position 175 (EU numbering) in CH1 is glutamic acid (E) or aspartic acid (D), and the amino acid residue at position 180 (EU numbering) in CL is glutamic acid (E) or aspartic acid (D); (a2) an amino acid residue at position 175 (EU numbering) in CH1 is lysine (K), histidine (H), or arginine (R), and an amino acid residue at position 180 (EU numbering) in CL is lysine (K), histidine (H), or arginine (R); (b1) an amino acid residue in CH1 in which the amino acid residue at position 175 (EU numbering) is glutamic acid (E) or aspartic acid (D), and an amino acid residue in CL in which the amino acid residue at position 131 (EU numbering) is glutamic acid (E) or aspartic acid (D); (b2) an amino acid residue at position 175 (EU numbering) in CH1 is lysine (K), histidine (H), or arginine (R), and an amino acid residue at position 131 (EU numbering) in CL is lysine (K), histidine (H), or arginine (R); (c1) the amino acid residues at positions 147 and 175 (EU numbering) in CH1 are glutamic acid (E) or aspartic acid (D), respectively, and the amino acid residues at positions 131 and 180 (EU numbering) in CL are glutamic acid (E) or aspartic acid (D), respectively; (c2) the amino acid residues at positions 147 and 175 (EU numbering) in CH1 are lysine (K), histidine (H), or arginine (R), respectively, and the amino acid residues at positions 131 and 180 (EU numbering) in CL are lysine (K), histidine (H), or arginine (R), respectively; (d1) an amino acid residue in CH1 at position 175 (EU numbering) is glutamic acid (E) or aspartic acid (D), and an amino acid residue in CL at positions 131 and 180 (EU numbering) is glutamic acid (E) or aspartic acid (D), respectively; (d2) the amino acid residue at position 175 (EU numbering) in CH1 is lysine (K), histidine (H), or arginine (R), and the amino acid residues at positions 131 and 180 (EU numbering) in CL are lysine (K), histidine (H), or arginine (R), respectively;

[0200] In addition to the above, the antigen-binding molecule of the present invention can further apply a technique (WO2013065708) for suppressing undesired association between CH1 and CL by introducing electric charge repulsion at the interface between CH1 and CL. Specifically, the antigen-binding molecule can be one having CH1 and CL, in which one or more pairs of amino acid residues selected from the group consisting of the pairs of amino acid residues shown in the following (a) to (d) are mutually electric-charge repulsive: (a) an amino acid residue at position 147 (EU numbering) contained in the heavy chain constant region (CH1) and an amino acid residue at position 180 (EU numbering) contained in the light chain constant region (CL); (b) an amino acid residue at position 147 (EU numbering) contained in CH1, and an amino acid residue at position 131 (EU numbering) contained in CL; (c) an amino acid residue at position 175 (EU numbering) contained in CH1, and an amino acid residue at position 160 (EU numbering) contained in CL. (d) an amino acid residue at position 213 (EU numbering) contained in CH1, and an amino acid residue at position 123 (EU numbering) contained in CL.

[0201] The antigen-binding molecules of the present invention can further apply a technique for suppressing unintended association between heavy chains by introducing electric charge repulsion at the interface of the second heavy chain constant region (CH2) or the third heavy chain constant region (CH3), a technique for suppressing unintended association between heavy chains and light chains by introducing electric charge repulsion at the interface between the heavy chain variable region and the light chain variable region, and a technique for suppressing unintended association between heavy chains and light chains by modifying amino acid residues that form a hydrophobic core present at the interface between the heavy chain variable region and the light chain variable region to charged polar amino acids (see WO2006 / 106905).

[0202] In the technique of suppressing unintended association between heavy chains by introducing electric charge repulsion into the CH2 or CH3 interface, examples of amino acid residues that contact the interface of other heavy chain constant regions include regions in the CH3 region corresponding to EU numbering positions 356 and 439, EU numbering positions 357 and 370, and EU numbering positions 399 and 409. The numbering of antibody constant regions was based on Kabat et al. (Kabat EA et al. 1991. Sequences of Proteins of Immunological Interest. NIH), and the numbering of heavy chain constant regions is shown in EU numbering.

[0203] More specifically, for example, an antigen-binding molecule comprising two types of heavy chain CH3 regions can be an antigen-binding molecule in which one to three pairs of amino acid residues selected from the pairs of amino acid residues shown in the following (1) to (3) in the first heavy chain CH3 region are mutually charge-repulsive: (1) amino acid residues at positions 356 and 439 (EU numbering) contained in the heavy chain CH3 region; (2) amino acid residues at positions 357 and 370 (EU numbering) contained in the heavy chain CH3 region; (3) Amino acid residues contained in the heavy chain CH3 region, which are amino acid residues at positions 399 and 409 (EU numbering).

[0204] Furthermore, the antibody can be one in which the combination of amino acid residues in a second heavy chain CH3 region different from the first heavy chain CH3 region is selected from the combination of amino acid residues shown in (1) to (3), and one to three combinations of amino acid residues corresponding to the combinations of amino acid residues shown in (1) to (3) that are mutually charge-repulsive in the first heavy chain CH3 region are not charge-repulsive with the corresponding amino acid residues in the first heavy chain CH3 region.

[0205] The amino acid residues described in (1) to (3) above are close to each other when associated. A person skilled in the art can find sites corresponding to the amino acid residues described in (1) to (3) above for a desired heavy chain CH3 region or heavy chain constant region by homology modeling using commercially available software or the like, and can appropriately modify the amino acid residues at those sites.

[0206] In the above-mentioned antigen-binding molecule, "electrically repulsive" or "having the same charge" means, for example, that two or more amino acid residues each have an amino acid residue included in either one of the above groups (X) or (Y).

[0207] In a preferred embodiment, the antigen-binding molecule may have a first heavy chain CH3 region and a second heavy chain CH3 region cross-linked via a disulfide bond.

[0208] In the present invention, the amino acid residues to be subjected to "modification" are not limited to those in the variable region of the antigen-binding molecule or the constant region of the antibody. Those skilled in the art can find amino acid residues that form an interface for a polypeptide mutant or a heterologous multimer by homology modeling or the like using commercially available software, and can modify the amino acid residues at the site so as to control the association. Homology modeling is one of the methods for predicting the three-dimensional structure of a protein using commercially available software. When constructing the structure of a protein with an unknown three-dimensional structure, first, a protein with a determined three-dimensional structure that is highly homologous to the protein is searched for. Next, the structure of the protein with an unknown structure is constructed using the three-dimensional structure as a template, and the structure is further optimized by molecular dynamics or the like, and the three-dimensional structure of the unknown protein is predicted.

[0209] In a technique for suppressing undesired association of heavy and light chains by introducing charge repulsion at the interface between the heavy and light chain variable regions, examples of amino acid residues that contact the interface between the heavy and light chain variable regions (VH) and VL include glutamine (Q) at position 39 (FR2 region) of the Kabat numbering on VH and glutamine (Q) at position 38 (FR2 region) of the Kabat numbering on the opposing (contacting) VL. Further examples of suitable residues include leucine (L) at position 45 (FR2) of the Kabat numbering on VH and proline (P) at position 44 (FR2) of the Kabat numbering on the opposing VL. The numbering of these sites is based on the literature by Kabat et al. (Kabat EA et al. 1991. Sequence of Proteins of Immunological Interest. NIH).

[0210] These amino acid residues are known to be highly conserved in humans and mice (J. Mol. Recognit. 2003; 16: 113-120). Therefore, for the association of VH and VL of antigen-binding molecules other than those shown in the Examples, the association of the variable regions of antigen-binding molecules can also be controlled by modifying the amino acid residues corresponding to the above amino acid residues.

[0211] Specifically, the antigen-binding molecule includes two or more amino acid residues that form the interface between VH and VL, which are mutually charge-repulsive amino acid residues. More specifically, the antigen-binding molecule includes one or two pairs of amino acid residues selected from the group consisting of the pairs of amino acid residues shown in (a) or (b) below: (a) an amino acid residue at position 39 of the Kabat numbering system contained in VH, and an amino acid residue at position 38 of the Kabat numbering system contained in VL; (b) the amino acid residue at position 45 in the Kabat numbering system contained in VH, and the amino acid residue at position 44 in the Kabat numbering system contained in VL.

[0212] The amino acid residues described in (a) or (b) above are close to each other when associated. A person skilled in the art can find sites corresponding to the amino acid residues described in (a) or (b) above for a desired VH or VL by homology modeling using commercially available software or the like, and can appropriately modify the amino acid residues at those sites.

[0213] In the above-mentioned antigen-binding molecule, the "mutually charge-repulsive amino acid residues" are preferably selected from amino acid residues included in either group (X) or (Y) below, for example: (X) glutamic acid (E) or aspartic acid (D), (Y) lysine (K), arginine (R) or histidine (H).

[0214] In a technique for suppressing undesired association of heavy and light chains by modifying amino acid residues that form a hydrophobic core at the interface between VH and VL to charged polar amino acids, suitable examples of amino acid residues that can form a hydrophobic core at the interface between VH and VL include leucine (L) at position 45 (FR2) of the Kabat numbering on VH and proline (P) at the corresponding position 44 (FR2) of the Kabat numbering on VL. The numbering of these sites was determined with reference to Kabat et al. (Kabat EA et al. 1991. Sequence of Proteins of Immunological Interest. NIH).

[0215] In general, the term "hydrophobic core" refers to the portion formed by the aggregation of hydrophobic amino acid side chains inside an associated polypeptide. Examples of hydrophobic amino acids include alanine, isoleucine, leucine, methionine, phenylalanine, proline, tryptophan, and valine. In addition, amino acid residues other than hydrophobic amino acids (e.g., tyrosine) may also be involved in the formation of the hydrophobic core. This hydrophobic core, together with the hydrophilic surface where the side chains of hydrophilic amino acids are exposed to the outside, is the driving force for promoting the association of water-soluble polypeptides. When hydrophobic amino acids from two different domains are present on the molecular surface and exposed to water molecules, the entropy increases and the free energy increases. Therefore, the two domains associate with each other to reduce and stabilize the free energy, and the hydrophobic amino acids at the interface are buried inside the molecule, forming a hydrophobic core.

[0216] It is believed that by modifying the hydrophobic amino acids that form the hydrophobic core when polypeptide association occurs to charge polar amino acids, the formation of the hydrophobic core is inhibited, and as a result, the association of the polypeptide is inhibited.

[0217] Those skilled in the art can determine the presence or absence of a hydrophobic core and the site (region) where the hydrophobic core is formed by analyzing the amino acid sequence of a desired antigen-binding molecule. That is, the antigen-binding molecule of the present invention is, for example, an antigen-binding molecule characterized in that amino acid residues capable of forming a hydrophobic core at an interface are modified to charged amino acid residues. More specifically, examples of the antigen-binding molecule include those in which either one of the following amino acid residues (1) and (2) is charged. The side chains of the amino acid residues shown in the following (1) and (2) are adjacent to each other and can form a hydrophobic core. (1) an amino acid residue at position 45 (Kabat numbering) contained in VH; (2) an amino acid residue at position 44 of the Kabat numbering system contained in VL;

[0218] Preferred examples of the charged amino acid residue in the antigen-binding molecule include glutamic acid (E), aspartic acid (D), lysine (K), arginine (R), and histidine (H). More preferred are glutamic acid (E) and lysine (K).

[0219] The amino acid residues described in (1) and (2) above are generally expressed as follows in humans and mice: (1) Leucine (L), (2) Proline (P) Therefore, in a preferred embodiment of the present invention, these amino acid residues are modified (e.g., substituted with an amino acid having a charge). The types of amino acid residues in (1) and (2) above are not limited to the above amino acid residues, and may be other amino acids corresponding to the amino acids.

[0220] Other known techniques can also be applied to the antigen-binding molecules of the present invention. For example, in order to promote the association between a first VH (VH1) and a first VL (VL1), and / or a second VH (VH2) and a second VL (VL2), an amino acid side chain in the variable region of one H chain can be replaced with a larger side chain (knob) and an amino acid side chain in the opposing variable region of the other H chain can be replaced with a smaller side chain (hole) so that the knob can be positioned in the hole, thereby promoting the association between VH1 and VL1, and / or VH2 and VL2, and as a result, further suppressing the association between VH1 and VL2, and / or VH2 and VL1 (WO1996 / 027011, Ridgway JB et al., Protein Engineering (1996) 9, 617-621, Merchant AM et al. Nature Biotechnology (1998) 16, 677-681).

[0221] For example, in the case of human IgG1, the following modifications can be made: Y349C and T366W to make the amino acid side chain in the CH3 region of one H chain a larger side chain (knob), and D356C, T336S, L368A, and Y407V to make the amino acid side chain in the CH3 region of the other H chain a smaller side chain.

[0222] Other known techniques can also be applied to the antigen-binding molecules of the present invention. By using a strand-exchange engineered domain CH3 in which a part of CH3 of one H chain of an antigen-binding molecule is replaced with a sequence derived from IgA that corresponds to that part, and the complementary part of CH3 of the other H chain is introduced with a sequence derived from IgA that corresponds to that part, the antigen-binding molecule of interest can be efficiently produced by complementary association of CH3 (Protein Engineering Design & Selection, 23; 195-202, 2010).

[0223] Other known techniques can also be applied to the antigen-binding molecules of the present invention. In producing a bispecific antibody, for example, a difference in isoelectric point can be imparted by adding different amino acid modifications to the variable regions of two types of H chains, and the bispecific antibody of interest can be produced by purifying the antibodies by ion exchange chromatography using the difference in isoelectric point (WO2007 / 114325).

[0224] In combination with the above technique, a technique of modifying t...

Claims

[Claim 1] The invention described herein.

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