Methods and compositions for selective modulation of protein expression
mts-siRNA target elements linked to transcribable DNA polynucleotides in transgenic plants enable selective protein expression reduction in male tissues, addressing the challenge of trait-specific regulation in male reproductive tissues.
Patent Information
- Application Number
- JP2025515366
- Authority / Receiving Office
- JP · JP
- Patent Type
- Applications
- Current Assignee / Owner
- Priority Date
- 2022-09-14
- Filing Date
- 2023-09-12
- Publication Date
- 2025-09-19
AI Technical Summary
Existing technologies lack effective methods for selectively regulating protein expression in male reproductive tissues of transgenic plants, particularly for conferring traits like pest resistance and herbicide tolerance, without affecting other plant tissues.
The use of male tissue-specific small interfering RNA (mts-siRNA) target elements operably linked to transcribable DNA polynucleotides, which induce sequence-dependent endonuclease cleavage of mRNA to reduce protein expression specifically in male tissues, such as tassels and pollen, by incorporating sequences with at least 90% identity to SEQ ID NO: 1.
This approach allows for selective regulation of protein expression in male reproductive tissues, enhancing traits like pest resistance and herbicide tolerance in transgenic plants while minimizing impact on other plant parts, thereby improving agronomic characteristics.