Multispecific t cell receptors
The method generates CD8+ T cells with multispecific TCRs using recombinant cytomegalovirus vectors, addressing the limitations of traditional TCRs by enabling broad antigen recognition and off-the-shelf treatment.
JP2026010019APending Publication Date: 2026-01-21OREGON HEALTH & SCI UNIV
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Patent Information
- Application Number
- JP2025169565
- Authority / Receiving Office
- JP · JP
- Patent Type
- Applications
- Current Assignee / Owner
- Priority Date
- 2019-12-16
- Filing Date
- 2025-10-07
- Publication Date
- 2026-01-21
AI Technical Summary
Technical Problem
Existing T cell receptors (TCRs) are limited by their specificity to a single peptide-MHC combination, requiring individual patient treatment and being ineffective against peptide mutations, and there is no method to generate TCRs with multiple specificities.
Method used
A method to generate CD8+ T cells with a multispecific TCR by administering recombinant cytomegalovirus vectors, identifying and isolating TCRs with desired specificities, and transfecting them into T cells to recognize multiple MHC/xenoantigen-derived peptide complexes.
Benefits of technology
Enables the generation of T cells with broad antigen recognition capabilities, overcoming limitations of traditional TCRs by allowing off-the-shelf treatment and effective targeting of diverse antigens.
✦ Generated by Eureka AI based on patent content.
Abstract
CD8 + T cells comprising multispecific T-cell receptors and methods for making the same are provided.SOLUTION: A method of generating CD8 + T-cells comprising multispecific T-cell receptors (TCRs), the method comprising: (a) administering to a subject a recombinant cytomegalovirus (CMV) vector comprising a first heterologous antigen-encoding nucleotide sequence; (b) identifying a first CD8 + TCR from a first set of the CD8 + T-cells; (c) administering to the subject a second heterologous antigen-encoding nucleotide sequence; (d) isolating one or more CD8 + T-cells from a second set of the CD8 + T-cells; (e) identifying a second CD8 + TCR from the second set of CD8 + T cells; (f) transfecting a third set of CD8 + T cells with an expression vector; and (g) selecting one or more of the third CD8 + TCRs having the highest avidity for a particular peptide of interest.SELECTED DRAWING: None
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