Method for producing medium composition for suspension culture of adherent cells

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By using water-insoluble polysaccharide nanofibers to carry extracellular matrix as carrier, the suspension of cultured cells without stirring is achieved, and the problem of low cell death and proliferation efficiency in the prior art is solved, simplifying the cell culture process and improving efficiency.

JP7647553B2Active Publication Date: 2025-03-18NISSAN CHEM CORP

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Patent Information

Application Number: JP2021529196
Authority / Receiving Office: JP · JP
Patent Type: Patents
Current Assignee / Owner
Priority Date: 2019-07-04
Filing Date: 2020-07-03
Publication Date: 2025-03-18
Estimated Expiration: 2040-07-03

Application Information

Patent Timeline

04 Jul 2019

Priority

03 Jul 2020

Application

JP2021529196

18 Mar 2025

Publication

JP7647553B2

IPC: C12N5/02

CPC: C12N5/0068; C12N5/0668; C12N5/0663; C12N5/0667; C12N2533/52; C12N2533/54; C12N2533/72; C12N2533/78

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Application Domain

Skeletal/connective tissue cells Mammal material medical ingredients

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AI Technical Summary

Technical Problem

The prior art has problems with low cell death and cell proliferation efficiency in cell culture, especially when using microcarriers. Due to the settlement of microcarriers under static conditions, stirring is required to cause cell collisions, and the cell separation treatment is complicated.

Method used

Water-insoluble polysaccharide nanofibers carry extracellular matrix as carrier. By adding the nanofibers carry extracellular matrix to the culture medium, cells can be suspended and cultured without stirring, and expanded culture by adding fresh culture medium to avoid cell detachment treatment.

Benefits of technology

Efficient suspension culture of cells is achieved, reducing cell death, simplifying the cell culture process, and improving cell proliferation efficiency.

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Abstract

The present invention provides a method for producing a culture medium composition for suspension culturing adherent cells, said method including the following steps:　(i) a step for supporting an extracellular matrix on nanofibers composed of water-insoluble polysaccharides; and (ii) a step for adding the nanofibers supporting the extracellular matrix, obtained in step (i), to a culture medium.

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