Reporter systems for radionuclide imaging
A novel reporter gene system with a short, easily cloneable construct for PET/SPECT and bioluminescence imaging addresses the limitations of existing PET/SPECT reporter genes, enabling precise tracking of therapeutic cells in vivo.
JP7862313B2Active Publication Date: 2026-05-19ERASMUS UNIV MEDICAL CENT ROTTERDAM ERASMUS MC
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Patent Information
- Application Number
- JP2022558260
- Authority / Receiving Office
- JP · JP
- Patent Type
- Patents
- Current Assignee / Owner
- Priority Date
- 2020-03-25
- Filing Date
- 2021-03-25
- Publication Date
- 2026-05-19
- Estimated Expiration
- 2041-03-25
AI Technical Summary
Technical Problem
Existing PET/SPECT reporter genes for tracking cells in therapies like CAR T-cell and oncolytic virus therapy are large, difficult to clone, and have limited specificity, leading to issues with imaging accuracy and duration.
Method used
A novel reporter gene system using a short, easily cloneable construct that encodes a fusion protein with a transmembrane domain and a split luciferase, allowing for PET/SPECT and bioluminescence imaging by expressing a reporter domain on the cell surface through a high-affinity peptide interaction.
Benefits of technology
Enables highly specific and efficient tracking of therapeutic cells in vivo using PET/SPECT and bioluminescence, providing accurate diagnostic and prognostic imaging for cell therapies.
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Abstract
The present invention provides: (i) a gene expression construct for expressing a reporter gene in a cell, wherein the reporter gene encodes a fusion protein comprising a transmembrane domain fused in-frame to a reporter domain, wherein the transmembrane domain, upon insertion of the fusion protein into the cell membrane, anchors the fusion protein to the cell membrane and simultaneously expresses the reporter domain on the cell surface; and (ii) a reporter system comprising a radiolabeled reporter peptide, wherein the reporter domain comprises a large polypeptide subunit of a split luciferase and the reporter peptide comprises a small peptide subunit of the split luciferase, and both subunits associate by complementation to form a luciferase complex.
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