Method for preparing antigen-delivery particle

By using a lysate containing a lysate solution containing a lysant, and by diluting the precipitated antigen components, the problem of difficulty in purification of antigen components in cell lysates is solved, and efficient and simple separation and purification of antigen components is achieved, improving the immune effect of the vaccine.

WO2025059964A1 Publication Date: 2025-03-27SUZHOU ERSHENG BIOPHARMACEUTICAL CO LTD
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Patent Information

Application Number
PCT/CN2023/120280
Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
Filing Date
2023-09-21
Publication Date
2025-03-27

AI Technical Summary

Technical Problem

It is difficult to efficiently isolate and purify antigenic components in cell lysates, especially in the preparation of cancer vaccines, and the purification methods of antigenic components are complex and inefficient.

Method used

One method is adopted to first lyse cells and/or tissues using a lysate containing a lysate, and then dilute the lysate to reduce the solubility of the protein and polypeptides, so that they precipitate, and then collect the precipitated portions by centrifugation for separation and purification.

Benefits of technology

This method easily and efficiently separates and purifies antigen components, avoids interference from substances such as sugars and DNA, improves the purity and load of antigen components, and enhances the immune effect of nanoparticles or microparticles.

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Abstract

The present invention relates to a method for preparing an antigen delivery particle. The method comprises firstly lysing cells and / or tissue by using a dissolving solution containing a dissolving agent; then dissolving the lysate component by using the dissolving solution; then diluting the dissolving solution in which the lysate is dissolved by using pure water or an aqueous solution, and thus allowing the precipitation of the protein polypeptide component due to the decrease in the content of the dissolving agent; performing centrifuging, and then dissolving the precipitated component for the second time by using the dissolving solution to obtain an antigen component resulting from separation and purification; and then loading the separated and purified antigen component onto the interior and / or surface of a nanoparticle or a microparticle, which nanoparticle or microparticle is the antigen-delivery particle. The antigen-delivery particle can be directly used as a vaccine, or can be used for activating other immune cells to prepare a cell vaccine, or can be used for activating other immune cells to assist with detecting, sorting and amplifying specific cells. The preparation method of the present invention is simple, rapid and low in consumption, and has a low solution viscosity during centrifugation so that a precipitate can be obtained without high-speed centrifugation.
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