Method for freezing mixture comprising biological material

By using a mixture of biological material and a carrier medium with matching osmotic pressure, and applying an alternating electric field during freezing, the method addresses the issue of water crystallization damage and energy inefficiency in traditional freezing techniques, achieving effective preservation of biological material.

WO2025062063A1 Publication Date: 2025-03-272 FRESH FINLAND OY
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Patent Information

Application Number
PCT/FI2024/050481
Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
Priority Date
2023-09-18
Filing Date
2024-09-16
Publication Date
2025-03-27

AI Technical Summary

Technical Problem

Freezing biological material often results in water crystallization, which damages the structure of cells and genes, and existing methods using liquid nitrogen or deep freezers are energy-intensive and pose safety risks.

Method used

A method involving a mixture of biological material and a carrier medium with osmotic pressure matching the biological material, subjected to an ambient temperature of -30 to 0 °C under an alternating electric field to prevent water crystallization during freezing.

Benefits of technology

This method effectively prevents water crystallization, thereby preserving the structure of biological material during freezing, and offers a safer and more energy-efficient alternative to traditional freezing methods.

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Abstract

The invention relates to a method for freezing a mixture comprising biological material. The mixture comprises a carrier medium having an osmotic pressure corresponding to the osmotic pressure of the biological material. The method comprises subjecting a sample of the mixture to an ambient temperature of -30 to 0 °C under the influence of an alternating electric field generated between electrodes to obtain a frozen mixture.
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