Cultivation method for herbicide-resistant, insect-resistant, and stress-tolerant soybeans jingdou 632 and jingdou 633, and use thereof

By introducing glyphosate-resistant, insect-resistant and reverse-resistant genes into the soybean explants, a complex trait transgenic soybean was obtained, which solved the problems of instability and poor resistance of transgenics, and achieved effective prevention and control of weeds, pests, drought and saline.

WO2025067526A1 Publication Date: 2025-04-03INSTITUTE OF CROP SCIENCE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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Patent Information

Application Number
PCT/CN2024/122070
Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
Priority Date
2023-09-28
Filing Date
2024-09-28
Publication Date
2025-04-03

AI Technical Summary

Technical Problem

The existing soybean transgenic technology has transgenic silencing, instability of exogenous genes, low expression, and the obtained regenerated plants are mostly chimera, which affects the progress of soybean genetic transformation.

Method used

The plant recombinant expression vector pGR20 was used to introduce the glyphosate-resistant gene g2m-epsps, insect-resistant gene cry1C and reversible gene GmNFYB1 into the explant of the receptor soybean mercury 02-69, and regenerated plants were obtained through the resistant clump bud induction, elongation bud induction and rooting stages.

Benefits of technology

Transgenic soybeans with complex traits can effectively prevent and control weeds and pests, and have high drought and salt-tolerant ability, solving the problems of instability and weak resistance of transgenics in the existing technology.

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Abstract

A cultivation method for herbicide-resistant, insect-resistant, and stress-tolerant transgenic soybeans, which belongs to the technical field of biology. A plant vector comprising herbicide resistance, insect resistance, and stress tolerance genes is utilized to introduce the glyphosate resistance gene g2m-epsps, the insect resistance gene cyr1C, and the stress tolerance gene GmNFYB1 into receptor soybean Hejiao 02-69, and a herbicide-resistant, insect-resistant, and stress-tolerant transgenic soybean is cultivated.
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