Solid supports and methods for strand invasion-based amplification
New grafting primers and clustering workflows address the challenges of primer dimers in nucleic acid amplification on solid supports, achieving faster clustering kinetics and higher sequencing intensity without altering library prep kits.
WO2025072683A1 Publication Date: 2025-04-03ILLUMINA INC
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Patent Information
- Application Number
- PCT/US2024/048868
- Authority / Receiving Office
- WO · WO
- Patent Type
- Applications
- Current Assignee / Owner
- Priority Date
- 2024-08-19
- Filing Date
- 2024-09-27
- Publication Date
- 2025-04-03
AI Technical Summary
Technical Problem
Existing methods for nucleic acid amplification on solid supports face challenges due to primer dimers, which hinder clustering workflows and limit surface primer density without a rapid drop in efficiency.
Method used
The development of new grafting primers that do not form unwanted hetero- and homo-dimers, along with novel clustering workflows, allows for faster clustering kinetics and higher sequencing intensity without requiring changes to library prep kits.
Benefits of technology
These solutions enable improved clustering results by reducing primer dimer formation, allowing for higher surface primer densities, and enhancing sequencing intensity, thus addressing the limitations of current technologies.
✦ Generated by Eureka AI based on patent content.
Abstract
This disclosure related to solid supports and methods for cluster amplification. In some embodiments, methods use a capture polynucleotide for seeding library fragments and lawn primers comprising clustering primer sequences.
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