Identification of active coronavirus infection by targeting the negative RNA strand
The method targets the negative RNA strand of coronaviruses using specific primers to differentiate infectious from non-infectious viruses, enhancing detection accuracy and containment efforts.
WO2025144655A9 Publication Date: 2025-08-21HOWARD UNIVERSITY
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Patent Information
- Application Number
- PCT/US2024/060772
- Authority / Receiving Office
- WO · WO
- Patent Type
- Applications
- Current Assignee / Owner
- Priority Date
- 2023-12-28
- Filing Date
- 2024-12-18
- Publication Date
- 2025-08-21
AI Technical Summary
Technical Problem
Current methods struggle to distinguish between replicating and non-replicating coronaviruses, particularly SARS-CoV-2, complicating containment efforts and quarantine decisions, as individuals may test positive for weeks without being infectious.
Method used
A method targeting the negative RNA strand of coronaviruses, using specific primers (e.g., SEQ ID NO: 1 and SEQ ID NO: 2) to differentiate between infectious and non-infectious viruses by amplifying the negative-sense RNA strand, which is essential for viral replication.
Benefits of technology
Enables early detection of active coronavirus infections, distinguishing replicating from non-replicating viruses, thereby improving containment strategies and reducing transmission.
✦ Generated by Eureka AI based on patent content.
Abstract
A method for detecting a coronavirus in a sample, the method containing i) isolating RNA from a sample; ii) contacting the isolated RNA with a first primer in the presence of a reverse transcriptase and dNTP under conditions permissive for cDNA synthesis, thereby generating cDNA; iii) contacting the cDNA with forward and reverse primers in the presence of a DNA polymerase and dNTP under condition permissive for cDNA amplification, thereby generating an amplified product, and iv) identifying the amplified product, thereby detecting the presence or absence of the virus, wherein the first primer binds to or hybridizes with a negative-strand RNA molecule produced by the virus.
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