Assay and method for determining antibody fragments in a sample
A dual detection system on a test strip addresses the challenge of quantifying antibody fragments by using separate zones for fragments and whole antibodies, ensuring accurate total antibody measurement.
WO2026110024A1 Publication Date: 2026-05-28INITO HEALTH INC
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Patent Information
- Application Number
- PCT/IB2025/061783
- Authority / Receiving Office
- WO · WO
- Patent Type
- Applications
- Current Assignee / Owner
- Priority Date
- 2024-11-19
- Filing Date
- 2025-11-18
- Publication Date
- 2026-05-28
AI Technical Summary
Technical Problem
Current lateral flow assays struggle to accurately detect and quantify antibody fragments due to their structural differences from intact antibodies, leading to compromised assay sensitivity and specificity.
Method used
A dual detection system on a test strip with distinct capture zones for antibody fragments and whole antibodies, using immobilized antibodies or binders to generate separate results, allowing comprehensive quantification by aggregating measurements from both zones.
Benefits of technology
Enables accurate and complete measurement of total antibody concentration by accounting for both fragmented and intact populations, overcoming the limitations of conventional assays.
✦ Generated by Eureka AI based on patent content.
Abstract
The present disclosure provides a method for determining the presence or quantity of antibody fragments in a sample by capturing said fragments on a line or zone comprising immobilized antibodies or binders specific to the antibody fragments The method may further comprise detecting whole antibody on a line comprising immobilized binders specific to the whole antibody. The immobilized binders specific to the antibody fragments may be immobilized on a test line of a test strip, and the immobilized binders specific to the whole antibodies may be immobilized on a control line of the test strip. The capturing of the antibody fragments results in generation of a first result, and the binding of the whole antibody results in generation of a second result. The method may determine total antibody concentration by aggregating measurements of antibody fragments and intact antibodies.
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