Bacteria TRIZ Cases

Specific amino acid deletions in alpha-amylase maintain enzyme activity during storage while preserving liquid composition properties.

Recombinant Escherichia coli strains biosynthesize tunable PHB-co-MCL copolymers from diverse substrates.

A polysaccharide carrier combined with specific amino acids stabilizes microorganisms during processing.

Optimized promoter sequences and stable genetic integration in engineered Escherichia coli boost 1,5-pentanediamine yield to 100-300 g/L.

Recombinant microorganism redirects acetyl-CoA flux to butyryl-CoA via gene deletion and overexpression.

SecM stalling selects stable scFv antibodies in the cytoplasm, resolving folding issues caused by reducing conditions.

A peptidoglycan-binding protein probe conjugated with a fluorescent material and quencher generates fluorescence upon binding to bacterial cell walls.

Knocking out LexA and acyl-ACP synthetase boosts fatty acid secretion, overcoming low productivity limits in biofuel production.

Pseudomonadales host cells direct recombinant crisantaspase to the periplasm using native signal sequences for high-level soluble production.

A single regulatory sequence drives both selectable marker and protein of interest in genetically modified bacterial cells.

Culturing Serratia microorganisms with optimized carbon sources resolves low productivity issues by enabling reliable alpha-hydromuconic acid production.

Adding glycerol, manganese, and uridine to culture media controls galactosylation levels to improve therapeutic efficacy consistency.

Segmented amine-quaternary ammonium buffers maintain stable pH from 2 to 13 while minimizing toxicity and metal-binding interactions.

Substituting histidine at position 407 desensitizes homoserine dehydrogenase to feedback inhibition, boosting fermentation yields.

A nutrient medium containing violuric acid and beef infusion supports simultaneous bacterial growth.

Lactobacillus paracasei GMNL-32 and L. reuteri strains inhibit inflammatory markers to treat autoimmune disease complications without side effects.

Isolated amidase from KNK AM 38 strain performs (R)-selective hydrolysis of racemic tropic acid amide without auxiliary reagents, ensuring high optical purity.

Multivariate statistical analysis identifies key metabolic fluxes affecting substance production.

A mutant nitrile hydratase Cal. t Nhase-A20V exhibits enhanced thermal stability and substrate tolerance for industrial biocatalysis.

Specific amino acid insertions in Taq DNA polymerase enhance reverse transcription activity and primer extension efficiency for long cDNA templates.

A continuous system using biological acid and modified hydrophobic nano silica to dissolve minerals and improve rock wettability.

Engineered microbes express heterologous transporters to resolve insufficient export efficiency and boost 2′ fucosyllactose production yields.

Purified polypeptides and recombinant microorganisms inhibit Staphylococcus aureus protease production through quorum sensing interference.

Two-stage pH cultivation selects acid-tolerant bacteria, preventing gas production and off-flavor while ensuring high viability.

Bacillus subtilis and Bacillus licheniformis strains stabilize rumen pH by converting lactate to acetate, preventing acidosis in dairy cattle.

Adding a flocculant aggregates lysed Bacillus cells, enabling dead-end microfiltration that improves flow rates and reduces filtrate turbidity.

Replacing ammonium chloride with ammonium hydroxide lowers ionic strength to resolve pH imbalances and boost ethanol space time yield.

A mixed lactic acid bacteria culture fluid with six strains and vitamin B1 inhibits rapid acidification to maintain kimchi flavor during storage.

Bead beating and enzyme cocktails overcome recalcitrant cell walls, increasing gDNA yield and diversity for agricultural microbiome analysis.

A phytoene synthase variant with an alanine to valine substitution at position 133 increases XMP or GMP production yields in Corynebacterium stationis.

A pro-polypeptide construct fused to the N-terminus of a target polypeptide enhances expression yields in prokaryotic cells.

Phosphorylated amino acid derivatives resolve solubility and stability bottlenecks, enabling high-concentration feed media for extended cell growth.

Bacillus coagulans spores in grain compositions survive high-temperature processing while retaining viability.

Optimizing cobalt concentration in the culture medium resolves production instability and ensures high carotenoid yield.

Modifying the ddh gene promoter enhances diaminopimelate dehydrogenase activity, resolving low enzyme levels that limit L-lysine production yields.

Targeted amino acid substitutions in region 66-98 extend biological half-life while maintaining receptor binding activity.

A Bacillus subtilis plasmid encodes Escherichia coli heat labile enterotoxin B-subunit using a secretory signal peptide.

Deleting edd and eda genes removes the Entner-Doudoroff pathway bottleneck, increasing L-tryptophan yield by 21-32% while maintaining glucose consumption.

Overexpressing SugR and knocking out nagA, gamA, and ldh redirects carbon flux away from by-products, resolving low production efficiency.

A Bacillus pumilus strain ferments glucose to produce tetramethylpyrazine.

Optimized promoters and glycerol kinase in recombinant Bacillus subtilis reduce protein synthesis pressure to reach 10.4 g/L N-acetylneuraminic acid.

Beneficial bacterial metabolites reduce Staphylococcus aureus growth and inflammation while enhancing skin barrier function to treat dysregulated microbiota.

Site-directed mutagenesis of ATP phosphoribosyltransferase overcomes histidine feedback inhibition to boost microbial fermentation productivity.

Combining Myxococcus xanthus with Bacillus amyloliquefaciens extends fermentation duration and increases biosurfactant concentration beyond pure culture limits.

BioCat-P inoculants solubilize insoluble soil phosphorus via organic acids, reducing synthetic fertilizer dependency and runoff pollution.

Heat-inactivated Parabacteroides goldsteinii resolves treatment complexity by preserving therapeutic efficacy while eliminating live organism risks.