Peptidoglycan-Binding Protein Probe for Rapid Bacterial Detection
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Solution Overview
Problem
Current methods for detecting bacteria, particularly in food and hospital environments, are inadequate for quick and specific identification of peptidoglycan-binding bacteria, often requiring additional enzymatic treatments and failing to distinguish between bacteria and yeast.
Innovation Solution
A probe comprising a peptidoglycan-binding protein (PGBP) conjugated with a fluorescent material and a quencher, which generates fluorescence upon binding to peptidoglycan, allowing for specific detection of bacteria without additional enzymatic treatments.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional bacterial detection methods are used, then bacteria can be detected, but additional enzymatic treatments are required and the process is complex
Solution Approach 1:
The invention combines the detection function and enzymatic treatment function into a single probe molecule. The probe contains both the peptidoglycan-binding component (for detection) and the beta-lactamase enzyme component (for enzymatic treatment), eliminating the need for separate treatment steps and reagents.
Solution Approach 2:
The probe is designed to perform multiple functions simultaneously: it can specifically bind to peptidoglycan for detection, hydrolyze beta-lactam antibiotics through beta-lactamase activity, and resist inhibition by beta-lactamase inhibitors. This multi-functionality simplifies the overall detection and treatment process.
2Measurement precision
If conventional detection methods are used, then bacteria can be detected, but it is difficult to distinguish between bacteria and yeast
Solution Approach 1:
The probe is designed with specific local properties that enable selective binding to peptidoglycan structures found in bacteria but absent in yeast. The peptidoglycan-binding domain is engineered to recognize specific chemical groups (such as N-acetylmuramic acid) that are unique to bacterial cell walls, providing high detection specificity.
3Measurement precision
If peptidoglycan-binding protein is used for detection, then specific detection is achieved, but the probe structure becomes complex
Solution Approach 1:
The probe uses a peptidoglycan-binding protein or peptide as an intermediary component that specifically recognizes and binds to peptidoglycan structures. This intermediary enables specific detection while allowing the rest of the probe structure to remain relatively simple, as the binding specificity is provided by the well-characterized peptidoglycan-binding domain.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The probe enables rapid and specific detection of both Gram-negative and Gram-positive bacteria, distinguishing them from yeast, and is suitable for various applications, including the food industry, without the need for additional enzymatic treatments.
Implementation Method 1
A probe comprising a peptidoglycan-binding protein (PGBP) conjugated with a fluorescent material and a quencher, which generates fluorescence upon binding to peptidoglycan
Data Source
AI summary
The present invention relates to a probe for detecting bacteria using a peptidoglycan-binding protein, and a method for preparing the same. Also, the present invention relates to a method for detecting bacteria using the probe.The probe for detecting bacteria according to the present invention can specifically detect bacteria. That is, the probe according to the present invention can clearly distinguish between yeast and bacteria and can detect both Gram-negative and Gram-positive bacteria, and thus is expected to be usable in various fields as a universal probe for detecting bacteria. Further, use of the probe allows bacteria to be detected by identifying only fluorescence development without an additional enzymatic treatment, thereby enabling a simple and quick bacterial detection. In particular, the probe is expected to be effectively usable in the food industry where quick bacterial detection is required.