Bacteria TRIZ Cases

Novel sporeforming probiotic strains produce NSPase enzymes to hydrolyze non-starch polysaccharides in fish diets.

Native gene knockouts in E. coli eliminate competing pathways, enabling high-titer succinic acid production in minimal salt media without complex nutrients.

Halophilic microbes degrade aniline and formate in hypersaline wastewater, enabling chloralkali process reuse without dilution.

Date palm extract broth replaces expensive soy peptones to lower production costs while sustaining microbial growth.

A recombinant Corynebacterium microorganism utilizes enhanced NCgl2522 activity to export putrescine.

Diluting bacterial populations isolates single carboxysomes for individual activity measurement using fluorescent enzyme labels.

Engineered Rhodopseudomonas palustris TIE-1 strains overexpress exogenous RuBisCo genes to synthesize polyhydroxybutyrate bioplastics.

A bacterial biopreparation uses alkaline extracts from poultry waste to support microbial growth without special storage needs.

Engineered microbes synthesize isopropanol alongside 1,4-butanediol through integrated metabolic pathways.

Recombinant lactic acid bacteria co-culture expressing bacteriocins controls microbial contamination during fermentation.

Lactobacillus plantarum postbiotics promote smooth muscle contraction and repair injured pelvic floor tissues.

Site-directed mutagenesis of nitrile hydratase improves thermal stability and substrate tolerance, resolving enzyme activity loss at high temperatures.

Microorganisms reduce ferric iron to solubilize nickel and cobalt, lowering costs and environmental impact.

Vacuum drying process stabilizes lactic acid bacteria cells with protective agents to maintain viability during storage.

A novel Streptococcus salivarius strain achieves high adhesion capacity to HEp-2 epithelial cells for effective upper respiratory tract colonization.

Segmented bacterial models resolve information loss from complex clinical isolates, enabling precise identification of antibiotic resistance mechanisms.

Segmented oligonucleotide pairs enable specific detection of the EF-2001 strain without cross-reacting with related bacteria.

Probiotic strains eliminate P. acnes infections without triggering antibiotic resistance or rebound sebum production via bacteriocin activity.

Reducing chamber pressure lowers the water boiling point, enabling spray drying of lactic acid bacteria at temperatures below 60°C to prevent thermal death.

Engineered microbes convert carbon sources into methacrylic acid, eliminating hazardous cyanide byproducts from chemical synthesis.

CBD-fused inclusion bodies concentrate bait and prey proteins, resolving low detection levels and false positives in living cells.

A reaction medium containing esterase substrates detects Streptococcus agalactiae through specific enzymatic hydrolysis reactions.

A novel promoter region drives high gene expression levels in Corynebacterium host cells.

An expression vector encoding the sPDZD2 tumor suppressor protein inhibits cancer cell proliferation and induces apoptosis in target tissues.

Microfluidic device creates oxygen gradient via permeable membrane for intestinal epithelium and vascular endothelium co-culture.

Inactivated Lactobacillus salivarius CJLS1511 cells degrade neutral lipids and adsorb endotoxins, resolving antibiotic resistance while improving immune status.

A microbial protein biomass mixture hydrates, emulsifies with oil, and homogenizes to form an egg replacement product.

Selected lactic acid bacteria degrade dietary purine in the digestive tract, preventing hyperuricemia without drug side effects.

Segmenting culture into growth and expression phases reduces host cell stress while increasing recombinant protein productivity.

Expressing proteases and lipases in Cupriavidus necator resolves the contradiction between high protein content and poor digestibility for feed applications.

Chimeric peptide links targeting and lytic modules to destroy EGFR-expressing tumors while sparing normal tissue.

Reducing aconitase activity via targeted mutations resolves the trade-off between bacterial growth and L-lysine production efficiency.

A microbial consortium degrades 1,2-dichloroethane to ethylene under anaerobic conditions.

A branched-chain amino acid permease variant with an alanine-to-threonine substitution enhances L-valine producing ability in Corynebacterium glutamicum.

Extracellular fluid from marine Bacillus sp. Q72 reduces sodium selenite, bypassing complex separation steps and bacterial debris contamination.

Eubacterium hallii bacteria produce pseudovitamin B12 in the intestine, bypassing compromised gastrointestinal absorption pathways.

UV-induced DagB gene mutation in Streptomyces coelicolor eliminates harmful enzyme activity to increase neoagarotetraose and neoagarohexaose yields.

Engineered microorganisms utilize enhanced dipeptide transporting proteins to export synthesized molecules from the cell interior.

Lactobacillus sp. KCCM 11826P replaces calcium binders, avoiding hypercalcemia and gastrointestinal side effects while controlling blood phosphorus.

Anionic exchange resins and chitosan recover high-purity heparosan from E. coli K5, resolving contamination risks in industrial manufacturing.

Hydrophobic organic phase contact enables recombinant cells to maintain aerobic metabolism and cell growth during alanine production.

Engineered microorganisms produce human milk oligosaccharides using inducible lysis and mutant lactose permease to increase yield.

A marker composition uses metabolic pathway genes to select living modified organisms without antibiotics.

Overexpressing formate dehydrogenase in Corynebacterium boosts NADH and ATP levels to drive putrescine biosynthesis.

Replacing native promoters in microbial cells boosts sucrose mutase activity and isomaltulose formation rates.

Engineering cell membranes increases surface area and vesicle formation, overcoming intracellular accumulation limits to boost hydrophobic material yields.

Biological cells autonomously mineralize metal ions into crosslinked shells, boosting compressive strength for artificial bone applications.

Microporous packing substrates in a multi-stage reactor support diverse microorganisms, resolving inefficiency in treating complex industrial wastewater.

Specific promoter sequences resolve low expression levels to enhance lipid production efficiency in Mortierella fungi.