Bacillus sp. Q72 Extracellular Fluid for Nanoselenium Biosynthesis
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Solution Overview
Problem
Existing biosynthesis methods for nanoselenium involve cumbersome separation steps, low separation efficiency, and high cytotoxicity due to bacterial debris contamination, limiting their application in food, biomedical, and feed additives.
Innovation Solution
A marine-derived nanoselenium-producing strain, Bacillus sp. Q72, is used to directly reduce high concentrations of sodium selenite using its extracellular fluid, resulting in a simple separation process with uniform particle size and low cytotoxicity nanoselenium production.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If simultaneous bacterial fermentation and nanoselenium synthesis is adopted, then nanoselenium can be produced, but the separation process becomes cumbersome and separation efficiency is low
Solution Approach 1:
The patent segments the bacterial culture into two distinct parts: intracellular components (containing bacterial debris) and extracellular components (containing nanoselenium). By using centrifugation to separate these two phases, the complex simultaneous fermentation-synthesis process is divided into manageable separation steps, enabling efficient nanoselenium recovery without cumbersome procedures
Solution Approach 2:
The patent extracts nanoselenium from the bacterial extracellular fluid through centrifugation and filtration. This extraction approach allows the nanoselenium to be separated from bacterial debris and other contaminants, achieving high separation efficiency while maintaining a relatively simple process flow
2Productivity
If simultaneous bacterial fermentation and nanoselenium synthesis is adopted, then nanoselenium can be produced, but bacterial debris contaminates the nanoselenium increasing cytotoxicity
Solution Approach 1:
The patent segments the bacterial culture into intracellular and extracellular phases, with nanoselenium being synthesized and released into the extracellular fluid. This segmentation naturally separates nanoselenium from bacterial debris, which remains in the intracellular phase, thereby reducing contamination and cytotoxicity
Solution Approach 2:
The patent extracts nanoselenium from the extracellular fluid through centrifugation and filtration, effectively removing bacterial debris and other contaminants. This extraction process produces clean nanoselenium with minimal contamination, significantly reducing cytotoxicity while maintaining production efficiency
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method achieves high reduction efficiency, simple separation, and produces nanoselenium with uniform particle size and low cytotoxicity, overcoming the limitations of traditional methods.
Implementation Method 1
using an extracellular fluid of the nanoselenium-producing strain for reducing sodium selenite to prepare the nanoselenium
Implementation Method 2
biosynthesis of nanoparticles has been widely welcomed owing to its cost-effectiveness, accessible source of raw materials, low toxicity and pharmacological potential
Data Source
AI summary
A marine-derived nanoselenium-producing strain and a method for preparing nanoselenium by using the strain are provided in the present disclosure, belonging to the field of nano biomaterials. According to the present disclosure, a Bacillus strain capable of preparing nanoselenium is screened from sediments taken from the Arctic Ocean, named Bacillus sp. Q72, which has been deposit in the China General Microbiological Culture Collection Center, and the deposit number is CGMCC No.26355. The extracellular fluid of this strain is used to directly reduce high concentrations of sodium selenite to obtain selenium nanoparticles.