Bacteria TRIZ Cases

Modular transposon vectors simplify marine bacterial engineering to boost TBP secretion for coral metamorphosis and reef restoration.

Viable methanotrophs plus oxygen agents raise rumen oxygen to cut methane emissions while improving nutritional efficiency.

Overexpressed aspDH in engineered E. coli boosts vitamin B5 fermentation yield while avoiding the pollution and waste burden of chemical synthesis.

A D372N NADP-dependent malic enzyme variant boosts carbon flux and improves L-glutamic acid production in Corynebacterium strains.

A Bifidobacterium breve strain converts linoleic acid into high-purity c9,t11-CLA, overcoming low yield and mixed-isomer limits.

Paenibacillus mucilaginosus activates CFB fly ash by dissolving silicates and reducing gypsum expansion, improving water demand and early strength.

Heat-killed Lactobacillus paracasei GMNL-346 and its supernatant inhibit oral cancer growth and stem cell self-renewal without toxicity to normal cells.

Cytofluorometry replaces slow plate counts to measure freeze-dried bacterial cell viability and cell wall integrity with repeatable precision.

By combining multiple microorganisms, this case shows how gut inflammation can be reduced through immune regulation and endocannabinoid modulation.

Two-stage metabolic control lowers key enzyme levels in stationary phase to relieve malonyl-CoA regulation and boost precursor flux.

Removing functional Tn904 from Agrobacterium strains prevents unwanted T-DNA insertions and antibiotic resistance spread during plant transformation.

Fermenting 2-hydroxy-3,3-dimethyl-4-oxobutanoic acid with bacteria or yeast avoids toxic reagents, high pressure, and excess waste.

Genetic mutations in Bifidobacterium and Lactobacillus improve wet and dry storage survival, cutting overages and production cost.

Enhanced malate thiokinase activity in methanotrophs improves PHB precursor formation and raises PHB production efficiency.

CO2-mediated diamine carbonate formation replaces acid-base salt steps, enabling cleaner free base isolation with higher purity and yield.

Targeted promoter nucleotide substitutions raise microbial glutathione yield, helping lower production cost for industrial use.

Magnetic-field treatment during microbial culture speeds Mn2+ oxidation and helps form biogenic manganese oxides with stronger pollutant adsorption.

Controlled BoNT/A variant ratios and optimized fermentation reduce truncated toxin species, limiting diffusion and improving tissue retention.

A probiotic Lactobacillus fermentum strain reduces fat accumulation, lowers fasting glucose, and improves insulin sensitivity with fewer side effects.

Triple knockout of alr1, alr2, and dat blocks bypass D-alanine synthesis in Staphylococcus, creating a controllable auxotrophic strain.

Air-dried biopolymer microcapsules protect lactic acid bacteria for dough use, improving baked-goods texture and sourdough-like qualities.

A probiotic Bifidobacterium bifidum strain activates GPR120 and boosts ATGL and HSL expression to lower glucose, insulin, and adipose gain.

Thermostable alanine dehydrogenase and glycolysis enhancement enable high-yield, optically pure L-alanine fermentation at 42-55°C.

A specific histidine export protein boosts microbial L-histidine secretion, easing biosynthesis bottlenecks and enabling scalable production.

A chitin-binding fusion hydrolase simplifies enzyme immobilization and cuts impurities, cost, and purification burden in ethylhexylglycerin production.

A polyurethane-degrading Acinetobacter strain breaks urethane N-H and C-N bonds, enabling cleaner waste plastic treatment without harmful by-products.

Synthetic signal peptides improve periplasmic secretion predictability and yield in Escherichia bacteria while supporting protein folding and easier purification.

Introducing alanine dehydrogenase into Corynebacterium enables high-yield L-alanine fermentation from low-cost carbon sources.

Boost PHA output by enhancing ClpB-family chaperone expression, helping transformed microorganisms maintain productivity under stress.

Using Lactobacillus sakei CVL-001 to activate NOD2, this case shows a lower-side-effect approach to controlling inflammatory disease symptoms.

Genome-integrated egtABCDE and a HisG E271K mutation raise ergothioneine yield from glucose while keeping fermentation simple for scale-up.

Biocatalytic dehydroxylation replaces chemical urolithin synthesis, enabling food-grade production with MV-activated enzymes and redox cofactors.

Combining pea fibre with Bifidobacterium longum improves insoluble fibre fermentability, raises short-chain fatty acids, and reduces gas discomfort.

Genetically tuned bacteria keep fixing atmospheric nitrogen in non-legume crops even with external nitrogen, reducing fertilizer dependence.

Waste lignin, biosurfactants, and catholyte improve hydrocarbon fouling removal while supporting more complete recovery from tanks and vessels.

A heat- and acid-tolerant L. coryniformis fermentation degrades Aflatoxin B1 across broad pH and temperature conditions while preserving food safety.

Specific YhhS residue changes improve O-phosphoserine export from microbial cells, lifting precursor supply for cysteine and derivative production.

A dual-promoter Lactococcus lactis vector boosts ASFV antigen expression and secretion, improving protein stability for oral infection prevention.

By integrating PEM1, PEM2, CKI, and CCT, this Bacillus subtilis strain produces CDP-choline from glucose without costly precursors.

Locked-on fimS promoters keep type 1 pilus expression stable in ADAS, improving mammalian cell targeting and biological agent delivery.

Heat-resistant Bacillus isolates keep enzyme activity through feed processing and the gut, improving poultry nutrient uptake and feed efficiency.

Targeted remodeling of plant-associated microbes boosts nitrogen fixation and crop colonization, reducing fertilizer waste and runoff.

Engineered cyanobacteria use plasmid-based metabolic pathways to convert CO2, glucose, or sucrose into ethylene glycol with lower process complexity.

Cheese whey is fermented into lactic acid and polymerized into PLA, cutting whey pollution while avoiding corn-based feedstock use.

Using Class 2 PhaC from Mycobacterium-related genera, this case raises C8+ 3HA content in PHA copolymers to improve low-temperature mechanics.

pepD and fadR mutations help an engineered strain overcome feedback-limited metabolism and raise tryptophan titer to 62.38 g/L.

A V140M biotin-protein ligase variant in Corynebacterium boosts L-glutamic acid production by improving enzyme activity and carbon flux.

Cell-free and engineered-strain biotransformation converts low-cost citramalate or citraconate into high-yield, high-purity L-2-ABA.

By fusing hydrogenase with photosystem I inside modified phototrophic cells, this case improves hydrogen output without the short life of in-vitro systems.

Purified multi-strain bacterial compositions replace fecal transplants to improve treatment consistency, lower infection risk, and restore gut function.