Bacillus Isolate Feed Composition for Stable Enzyme Activity

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Solution Overview

Problem

Existing animal feed supplements, particularly biosynthetic enzymes, face issues with instability during feed processing and in the animal gut, leading to inefficient processing of anti-nutritional factors and high production costs.

Innovation Solution

Utilization of stable Bacillus isolates, such as Isolate 46 and Isolate 40, which are heat-resistant and produce multiple enzymes to break down anti-nutritional compounds, such as cellulose, xylans, and are added to the feed compositions.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If biosynthetic enzymes are added to animal feed to break down anti-nutritional factors, then nutrient digestibility is improved, but enzyme stability during feed processing and in the gut deteriorates

Engineering Contradiction:

Improveenzyme stabilityVSAvoidnutrient digestibility

Core Design Contradiction:

ReliabilityVSProductivity

Solution Approach 1:

The patent applies preliminary action by pre-coating enzymes with protective materials (such as starch, protein, or synthetic polymers) before feed processing. This protective coating is applied in advance to shield enzymes from denaturation during subsequent heat treatments and to protect them from degradation in the acidic gut environment, thereby maintaining enzyme stability while preserving their digestive function.

Inventive Principle:

Principle #10Preliminary action

Solution Approach 2:

The patent employs parameter changes by modifying the physical and chemical properties of enzymes through coating treatments. The coating process alters the surface characteristics, thermal stability, and pH resistance of enzymes, enabling them to withstand feed processing conditions and gut environment without losing their catalytic activity for breaking down anti-nutritional factors.

Inventive Principle:

Principle #35Parameter changes

2Adaptability or versatility

If biosynthetic enzymes are used to process anti-nutritional factors, then feed ingredient flexibility is improved, but production cost increases

Engineering Contradiction:

Improvefeed ingredient flexibilityVSAvoidproduction cost

Core Design Contradiction:

Adaptability or versatilityVSEase of manufacture

Solution Approach 1:

The patent applies the principle of using cheap protective materials for enzyme coating, such as starch, protein, or other inexpensive polymers. These coating materials provide adequate protection at low cost, making the enzyme supplement economically viable while maintaining feed ingredient flexibility. The coating materials are selected based on cost-effectiveness rather than maximum performance.

Inventive Principle:

Principle #27Cheap short-living objects (Disposable)

Solution Approach 2:

The patent employs universal coating materials that can protect multiple different enzymes against various degradation mechanisms (heat, acid, proteases). The same coating approach can be applied to different enzyme types and feed formulations, providing a versatile solution that maintains ingredient flexibility while controlling production costs through a standardized protection strategy.

Inventive Principle:

Principle #6Universality (Multi-functionality)

3Object-affected harmful factors

If heat treatment is applied during feed processing to ensure safety, then pathogen elimination is improved, but enzyme activity deteriorates

Engineering Contradiction:

Improvepathogen eliminationVSAvoidenzyme activity

Core Design Contradiction:

Object-affected harmful factorsVSReliability

Solution Approach 1:

The patent applies beforehand cushioning by providing a protective coating on enzymes before they are exposed to heat treatment during feed processing. This pre-applied protective layer acts as a cushion or shield that absorbs the thermal stress, preventing direct denaturation of the enzyme structure while still allowing the heat treatment to effectively eliminate pathogens in the feed.

Inventive Principle:

Principle #11Beforehand cushioning (Prior cushioning)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Improves nutritional uptake and reduces the incidence of footpad dermatitis in poultry, enhancing growth rate and feed efficiency while lowering production costs by utilizing naturally occurring, non-GMO Bacillus isolates that remain stable during feed processing and in the gut.

Implementation Method 1

produce multiple enzymes to break down anti-nutritional compounds, such as cellulose, xylans

Methodology Applied

Scientific EffectEnzyme: Enzyme

Data Source

PatentUS20250367243A1Bacillus isolate compositions and methods of using and producing the same

Publication Date: 2025.12.04 THE BOARD OF TRUSTEES OF THE UNIV OF ARKANSAS

AI summary

The present invention provides Bacillus isolates, as well as probiotic and animal feed compositions comprising said isolates, which may be included in the diet of poultry to improve growth performance with reduced energy diets. Methods of using these compositions to improve nutritional uptake or to reduce the incidence of footpad dermatitis in poultry are also provided.