Bacteria TRIZ Cases

Engineered attenuated Gram-negative bacteria use T3SS and targeting switches to stably deliver therapeutic proteins into cancer cells.

Combining Enterococcus faecium SF68 with butyric acid helps restore intestinal uptake under inflammation, improving enterocyte health and animal wellness.

Specific amino acid changes in aldehyde dehydrogenase improve hydroxybutyryl-CoA conversion and raise renewable butanediol intermediate yields.

Electroporated CRISPR RNP, recombinases, and phosphorothioated donor DNA improve recombination in Gram-positive and lactic acid bacteria.

Engineered OMVs display carbonic anhydrase on their surface to control vesicle size, reduce batch variability, and convert CO2 into metal carbonates.

Asymmetric division genetic circuits maintain stem and factory cell ratios, boosting fermentation yield while reducing culture renewal and induction cost.

A Lactobacillus-fermented curdling ingredient speeds milk coagulation, limits acidic off-flavor, and adds bioavailable calcium.

Site-directed argD mutations raise acetylornithine aminotransferase activity, boosting L-arginine or L-citrulline yield in Corynebacterium.

An optimized H. pylori culture medium uses urea, phenol red, and selective additives to speed resistance testing with visible color change.

Lysozyme- or chitinase-treated gram-positive bacterial cell walls boost spiciness and kokumi without relying on higher spice levels.

A fluorescence biosensor plasmid links D-lactic acid output to strain screening, enabling faster gene mining and higher-yield Zymomonas mobilis selection.

An exogenous Class 2 PhaC gene enables microorganisms to make PHA copolymers richer in C8+ 3HA units for better low-temperature mechanics.

Heavy ion mutagenesis and screening produced a stable Xanthomonas campestris strain that raises xanthan yield and solution transparency.

A hydrogel-mediated nanotransfer process bonds lithographic nanopatterns to live cells and tissues without solvents, heat, or pressure.

Weakening GNAT acetyltransferase in Corynebacterium boosts O-acetyl homoserine fermentation for lower-cost L-methionine production.

Anti-CRISPR proteins suppress Cas9 activity across bacterial CRISPR systems, enabling controlled editing and higher virulent phage titers.

Protein variants from Corynebacterium improve IMP export in microorganisms, raising fermentation yield and easing product recovery.

Fluorescent sensor proteins and microbial spores enable fast, reliable oxidative sterilization checks without complex enzymatic assays.

An amino acid substitution at the 86th position of glutamate-cysteine ligase boosts microbial glutathione yield while lowering production cost.

Engineered sucrose phosphorylase replaces complex chemical synthesis with biocatalysis to streamline nucleoside analogue production.