Bacteria Peptides TRIZ Cases
- Microbial HMO Export Engineering for High-Purity Oligosaccharides
Engineered sugar exporters boost secretion of complex HMOs while limiting precursor carryover, enabling cleaner large-scale recovery.
- Protein-Based MnII Sensor for Selective Real-Time Metal Imaging
A LanM-derived FRET sensor detects MnII in real time while avoiding calcium and magnesium interference for cellular metal imaging.
- Beta-Galactosidase Variants for High-Temperature Acidic Lactose Hydrolysis
Engineered beta-galactosidases stay active at low pH and high heat, enabling lactose hydrolysis in UHT and fermented dairy processing.
- Near-Infrared Optical Switch Proteins for Reliable Binding Control
Mutant RpBphP1 and RpPpsR2 proteins weaken dark-state binding and strengthen near-infrared-induced association for reliable photoswitching.
- Beta-Galactosidase Stability Across pH and Heat in Dairy Processing
Acid- and heat-stable beta-galactosidases enable lactose hydrolysis through pH shifts and high-temperature dairy processing.
- Metal-Binding Protein for Selective Rare Earth Separation
Selective metal-binding proteins capture trivalent and tetravalent rare earth ions, enabling cleaner sensing and separation than harsh extraction methods.
- Cysteine-Less Split Inteins for Reducing-Agent-Free Bioconjugation
Engineered cysteine-less split inteins enable reducing-agent-free bioconjugation while preserving extein functionality across broad reaction conditions.
- Engineered Imine Reductases for Stereoselective Reductive Amination
Directed-evolved imine reductases enable one-step, stereoselective synthesis of chiral secondary and tertiary amines from ketones and amines.
- Recombinant Catalase and DPS Vaccines for Catfish Columnaris
Recombinant catalase and DPS proteins replace variable live or bacterin vaccines, improving catfish resistance to Flavobacterium covae infection.
- HMO Export in Engineered Microbial Hosts for Fermentation Purity
Selective YjhB or SetA export moves complex HMOs into culture broth while retaining precursors to improve fermentation purity.
- Amino Acid-Specific ClpS Binders for N-Terminal Leucine Detection
Engineered ClpS sequence variants enhance N-terminal leucine affinity for iterative, high-fidelity protein sequence determination.
- Engineered Imine Reductases for Stereoselective Chiral Amine Synthesis
Directed-evolution imine reductases convert ketones and amines into chiral secondary and tertiary amines with broad substrate scope.
- Sso7d Glycan-Binding Protein Scaffolds for Monosaccharide Selectivity
Directed evolution turns Sso7d into compact glycan-binding proteins for selective monosaccharide and disaccharide recognition.
- Ochrobactrum-mediated plant transformation via VirD2-dependent T-DNA transfer
Ochrobactrum strains mediate polynucleotide transfer to plant cells using VirD2-dependent mechanisms for stable genomic integration.
- Vi Conjugate Vaccine Manufacturing via Carbodiimide Activation
Carbodiimide activation of Vi polysaccharides enables conjugation with CRM197 carriers, establishing immunological memory in infants and young children.
- Lipidated Protein Production in E. coli via Genetic Adaptation
Adapting E. coli strains enables high-level expression of lipidated proteins for vaccine development.
- FAST Tag Reversible Chromophore Binding for Rapid Fluorescent Labeling
A Photoactive Yellow Protein derivative binds fluorogenic chromophores reversibly to enable rapid fluorescent labeling of cellular proteins.
- Allulose Epimerase Variant for High-Temperature Fructose Conversion
Engineered allulose epimerase variants substitute specific amino acids to boost fructose conversion rates.
- Recombinant E. coli Microcystin Production via PPTase
Decoupling synthesis from slow cyanobacterial growth via phosphopantethienyl transferase mediation boosts yield and simplifies purification.
- Optogenetic Membrane Potential Probes via Microbial Rhodopsins
Microbial rhodopsins replace invasive electrodes with optical fluorescence signals to measure membrane potential non-invasively.
- Solid Phase TALE Assembly via Magnetic Bead Anchoring
Magnetic bead anchoring anchors biotinylated DNA fragments for direct verification, eliminating intermediate amplification and reducing plasmid complexity.
- Engineered Heme Protein Catalysts for Carbon-Boron Bond Formation
Directed evolution of heme proteins creates biocatalysts that form carbon-boron bonds with high enantioselectivity.
- Untargeted Metabolomic Screening for Fungal Biosynthetic Gene Clusters
Segmenting untargeted screening rounds and applying feedback loops resolves the trade-off between metabolite identification rates and data analysis complexity.
- Chimeric REMAX Receptors for Xanthomonas Recognition
Chimeric pattern recognition receptors combine REMAX extracellular domains with heterologous signaling tails to detect specific bacterial molecular patterns.
- Bicyclic Peptide Conjugates Targeting Penicillin-Binding Proteins
Conjugating bicyclic ligands to carrier peptides overcomes limited cell entry, boosting killing efficacy against wild-type bacteria.
- Modified Geobacter Pilus Conductivity Diameter
Aromatic amino acid substitution in the pilus monomer boosts conductivity one million-fold while reducing nanowire diameter to 1.5 nm.
- Circadian Clock Modulation for Continuous Gene Expression
Overexpressing KaiA or KaiC proteins switches cyanobacteria from rhythmic cycling to continuous production, resolving low heterologous expression limits.
- PSR1 Transcription Factor Overexpression in Microalgae Lipid Production
Constitutive PSR1 expression uncouples lipid accumulation from nutrient stress, maintaining cell growth while boosting biofuel precursor yields.
- Glutathione Production via Extracellular Transport in E. coli
Engineered E. coli strains export glutathione using specific transporter proteins for direct medium recovery.
- Mutated PPO Polypeptides for Herbicide Tolerance
Mutated PPO polypeptides confer herbicide tolerance through specific amino acid substitutions.