DNA/RNA Fragmentation TRIZ Cases
- Synthetic Lariat RNA for Nuclease-Resistant Gene Silencing
Lariat RNA structures resist serum nucleases and maintain 70% potency after seven days, solving rapid degradation in traditional siRNA therapies.
- 18-mer RNAi Agent Internal Spacers Nuclease Resistance
Internal spacers in an 18-mer RNA interference agent improve nuclease resistance and activity duration compared to standard siRNA formats.
- SGLT2 dsRNA Agents with Cholesterol Conjugation
Cholesterol-conjugated double-stranded RNA agents silence SGLT2 transcripts while resisting nuclease degradation in vivo.
- LNA Gapmer DUX4 mRNA Suppression for Muscular Dystrophy
LNA gapmers hybridize with DUX4 mRNA to suppress expression, addressing the lack of curative options for Facioscapulohumeral Muscular Dystrophy.
- Antagomir Oligonucleotides for miRNA Inhibition
Lipophilic conjugated oligonucleotides inhibit specific microRNAs while resisting nucleolytic degradation during systemic circulation.
- C3 iRNA Agents for Ocular and Neurodegenerative Disease Treatment
GalNAc-conjugated RNAi compositions silence C3 transcripts, avoiding invasive procedures and side effects like cataracts.
- Single-Cycle Aptamer Selection Using DNase I Digestion
DNase I digestion removes weak binders during single-cycle selection, preserving sequence diversity and reducing process time compared to multi-round SELEX.
- Inhibitory Nucleic Acids Displace PRC2 From Chromatin
Modified oligonucleotides target nuclear lncRNAs to displace PRC2, resolving inefficiency in regulating gene expression for neurodegeneration therapies.
- ACE-tRNA Circular DNA for Premature Termination Codon Readthrough
Circular ACE-tRNAs revert premature termination codons without ototoxicity or missense mutations.
- Root-Preferred Promoter Sb-TIP2-3 for Tissue-Specific Gene Expression
Sb-TIP2-3 promoter sequences drive tissue-specific expression in plant roots, resolving the contradiction between continuous activity and spatial control.
- Chromatin-Associated RNA Fragment Modulating Plant Stress Response
RNA fragment with stem-loop structure induces defense gene expression to prevent bacterial resistance in plants.
- siRNA Design for rpS3 Inhibition via Experimental Screening
Experimental screening of candidate sequences resolves the contradiction between rapid computational design and reliable inhibitory effectiveness.
- HNF4α-P2 Isoform Silencing via siRNA for Liver Failure
siRNA silences HNF4α-P2 isoforms to treat liver failure, addressing ineffective conventional therapies that fail to improve hepatocyte function.
- TREMs Modulate tRNA Pools for Mutant Codon Translation
TREMs pair with mutant codons to increase isoacceptor tRNA abundance, restoring protein translation efficiency despite genetic variations.
- Circular Tau-TfR Aptamers for Blood-Brain Barrier Penetration
Circular Tau-TfR bispecific aptamers cross the blood-brain barrier via transferrin receptor-mediated transcytosis, reducing Tau protein levels in brain tissue.
- Segmented siRNA with 3' Overhangs for Targeted Gene Silencing
Segmented siRNA strands self-assemble into double-stranded structures with 3' overhangs to enhance stability and inhibit target mRNA levels.
- Hairpin RNA Mismatches for Gene Knockdown Efficiency
Recombinant nucleic acid molecules form hairpin structures with specific mismatches to enhance RISC incorporation and gene knockdown.